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Changes in Chemical Composition and Biological Activities of , Oriental Crude Drugs by Food. Processing Techniques (II) - Changes in Paeonol Contents in Roasted Moutan Cortex - (식품학적 가공에 의한 생약의 성분 및 활성 변화 (II) - Roasting 처리에 의한 목단피 (Moutan Cortex) 에탄올 추출물 중 Paeonol의 함량변화 -)

  • Jeon, So-Young;Kim, Eun-Kyung;Kwak, Hye-Min;Kim, Ja-Young;Lim, Jung-Hyun;Chung, Shin-Kyo;Song, Kyung-Sik
    • Korean Journal of Pharmacognosy
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    • v.35 no.4 s.139
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    • pp.388-392
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    • 2004
  • The paeonol content in roasted Moutan Cortex was increased about three times compared to that of untreated one. The paeonol content reached at its maximum level $(454.3\;{\mu}g/mg\;ethanol\;extract)$ after roasting at $190^{\circ}C$ for 30 minutes. Roasting processing did not affect on the DPPH radical scavenging activity of Moutan extracts.

Quantitative Analysis of Chrysin and Pinocembrin in Korean Propolis (국산 프로폴리스 내 Chrysin과 Pinocembrin의 정량분석)

  • Kim, Se Gun;Hong, In Pyo;Woo, Soon Ok;Jang, Hye Ri;Han, Sang Mi
    • Korean Journal of Pharmacognosy
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    • v.48 no.1
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    • pp.88-92
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    • 2017
  • In the present study, we carried out quantitative analysis of chrysin and pinocembrin in Korean propolis by ultra performance liquid chromatography (UPLC) equipped with diode array detector. The separation was done using BEH C18 ($2.1{\times}50mm$, $1.7{\mu}m$) column with a mobile phase consisting of MeCN and 0.1% $H_3PO_4$ at 280 nm. The chromatographic method was validated for specificity, limit of detection, limit of quantification, linearity, precision, and accuracy. A quantitative analysis exhibited that the contents of the two compounds in Korean propolis collected from 8 inland areas except Jeju-do ranged from 3.1-46.0 mg/g. These results will be valuable as basic data for standardization of Korean propolis.

Effects of Heavy Metals on Plant Growths and Pigment Contents in Arabidopsis thaliana

  • Baek, Seung-A;Han, Taejun;Ahn, Soon-Kil;Kang, Hara;Cho, Myung Rae;Lee, Suk-Chan;Im, Kyung-Hoan
    • The Plant Pathology Journal
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    • v.28 no.4
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    • pp.446-452
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    • 2012
  • The effect of heavy metals on seedling growth and pigment levels was studied in Arabidopsis using essential (Cu, Mn, and Zn) and non-essential metals (Pb and Hg). Generally increasing the concentrations of the metals resulted in a gradual decrease in root and shoot lengths, a decrease in chlorophylls, an increase in anthocyanins and a fluctuation in carotenoid content depending on the metal types. The toxicity of the metals decreased in the following order: Cu > Hg > Pb > Zn > Mn. Among the five metals, Cu was exceptionally toxic and the most potent inducer of anthocyanins. Pb induced the smallest quantity of anthocyanins but it was the strongest inducer of carotenoids. It suggests that the Cu-stressed Arabidopsis may use anthocyanins as its main antioxidants while the Pb-stressed Arabidopsis use carotenoids as its main protectants. All of the five metals induced an accumulation of anthocyanins. The consistent increase in anthocyanin content in the metal-stressed Arabidpsis indicates that anthocyanins play a major role in the protection against metal stresses.

Effect of extraction conditions on chemical composition and antioxidant properties of mulberry fruit

  • Lee, Sora;Koo, Bonwoo;Ju, Wan-Taek;Kim, Hyun-Bok;Kweon, HaeYong;Lee, Ji Hae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.42 no.2
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    • pp.25-32
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    • 2021
  • Mulberry fruit (Morus alba L.) contains phytochemicals, including 1-deoxynojirimycin, quercetin-glucoside, kaempferol-glucoside, and anthocyanins, which have antioxidant effects. In this study, mulberry fruit extract was prepared at various temperatures (25-100℃) and water/ethanol solvent concentrations (0%-100% ethanol). Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism (CD) data indicated that the content of bioactive compounds such as polyphenols and flavonoids was lower in 100% ethanolic extracts than in 30%-50% ethanolic extracts. Radical scavenging activity determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-di-3-ethylbenzthiazoline sulfonic acid (ABTS) assays was highly correlated with polyphenol and flavonoid content. In conclusion, 30%-50% ethanolic extracts contained the highest contents of bioactive compounds and exhibited high levels of radical scavenging activity. These findings may inform the use of mulberry fruit extract as a functional food.

Effects of Air Pollution on Precipitation and Living Organisms in Seoul Area(3. Contents of S, Pb and Cd in Litters of Ginko biloba) (서울 地域의 大氣汚染이 降水와 生物에 미치는 영향 3. 地域別 은행나무 낙엽의 S,Pd 및 Cd 의 함량)

  • Chang, Nam-Kee;Lee, Kyung-Hyung
    • The Korean Journal of Ecology
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    • v.13 no.3
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    • pp.165-172
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    • 1990
  • The contents of Pb and Cd in litters of Ginko biloba collected from thirty-four areas in Seoul were measured and analyzed for among relative air pollutions. S contents in litters of G. biloba were 0.88% at Hyehwadong, 0.98% at Yongsan and 0.95% at Ch angdong. Mean value were 0.65% in 0-10 km areas from Kwanghwamun, but were 0.47% in 10-15 km areas. In S contents, the correlation between 0-5 km area and 10-15 km area from Kwanghwamun was high significant, but correlation between 0-5 km area and 5-10 km area from Kwanghwamun had no significance. Pb contents were 118.95 ppm at Pildong, 112.22 ppm at Ulchiro 3-ga and 105.55 ppm at Bus terminal (Banpo). In Pb contents the correlation between 0-5 km area and 10-15 km aea from kwanghwamun was high significant, but the correlation between 0-5 km area and 5-10 km area from Kwanghwamun had no significance. In Cd contents. Cd contents were high in Youido, Shinch on, Kongdokdong, Haengdangdong, Kirum, Ch ongnyangni and Imundong.

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On Lead Contamination of Roadside Trees in Jinju City (진주시 가로수의 연(Pb) 오염에 관하여)

  • 성민웅
    • Journal of Plant Biology
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    • v.19 no.4
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    • pp.107-110
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    • 1976
  • This study was carried out to investigate lead contamination in the leaves of roadside trees and the cause of yellow-brown symptom of leaves in Jinju and Daegu city. After the leaves were collected and dried in 105$^{\circ}C$ drying oven from September to October in 1974, the samples were used for analysis of the lead contents in 1976. The roadside trees in the two cities were mostly the four kinds of Ginkgo biloba L., Thuja orientalis L., Platanus orientalis L. var. acerifolia AIT. and Prunus pseudo-cerasus $LINDL{\times}Prunus$ subhirtella MIQ. The lead contents in the leaves of roadside trees in Daegu city was higher than that of Jinju city. The lead contamination of the evergreen trees of roadside was higher than that of the deciduous trees. In Jinju city the site of the highest contents of lead in the leaves was revealed with Jinju station(0.73ppm), the next site was revealed with the road(0.16ppm) between Namgang bridge and Jinju middle school, and the lowest site was generally beside the roads of suburbs (0.07ppm). Because of the low contents of lead in the leaves, it was found that the yellow-brown symptoms of leaves were not caused by the lead toxicity.

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Effect of Anaerobic Treatment on Carbohydrate-Hydrolytic Enzyme Activities and Free Amino Acid Contents in Barley Malt

  • Yun, Song-Joong;Choi, Kyeong-Gu;Kim, Jin-Key
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.1
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    • pp.19-22
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    • 1998
  • Effects of acute anoxia on carbohydrate hydrolytic enzyme activities and free amino acid contents in malt were examined. Malts were prepared with barley grains germinated for 7 days which contained the highest levels of amylolytic and(1-3,1-4)-$\beta$-glucanase activities. $\alpha$-Amylase and $\beta$-amylase activities in malts were not significantly affected by anoxia for 5 or 10 h.(1-3,1-4)-$\beta$-Glucanase activity, however, decreased about 7 to 10% by anoxia for 5 or 10 h. Alanine and $\gamma$-aminobutyric acid content changed drastically. Alanine contents in malts increased by 2.2- and 2-fold, and $\gamma$-aminobutyric acid contents by 1.4- and 1.9-fold under anoxia for 5 and 10 h, respectively.

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Changes in Protein Contents and Activities of Proteolytic Enzymes in Medicago sativa During Regrowth

  • Kim, Tae-Hwan
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.357-363
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    • 1994
  • An expreiment with non-nodulating alfalfa (Medicago sativa L.) plants was designed to investigate the changes in protein contents and the activities of proteolytic enzymes during a regrowth period of 24 d. Shoot removal caused a depression of root growth and significantly reduced protein contents in roots. An initial decline of root proteins for the first 10 d was followed by a rapid recovery from d 11 to 24. The major increase of regrowing shoot weight occurred also from d 11. The activities of aminopeptidase and endoprotease slightly decreased in regrowing leaves, while protein contents remains stable after shoot removal. Roots exhibited source behaviour with a rapid increase of endoprotease activities for the first 10 d of regrowth; about a 370% increase over the initial level was observed. Increase in endoprotease activity in roots coincided with the time of protein remobilization after shoot removal, indicating the important role of endoproteases in protein degradation.

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Effects of Nitrogen Nutrient on the Yield, Protein, Amino acid, Chlorophyll, Carotene, RNA, and DNA Contents in Rye-Grasses (Rye-grass류의 물질생산, 단백질, aminotks, 엽록소, Carotene, RNA 및 DNA의 함량에 미치는 질소의 영향)

  • 장남기
    • Journal of Plant Biology
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    • v.16 no.1_2
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    • pp.30-38
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    • 1973
  • To study the response to plant growth by the environmental factors, the effects of application of nitrogen on changes in the yield, crude protein, amino acids, chlorophyll, carotene, total phosphorus, acid-soluble phosphorus, phospholipids, RNA, and DNA were investigated with westerworlds 9Lolium sublatum) and perennial rye-grasses (Lolium perenne). The amounts of dry weight, crude protein, amino acids, chlorophyll, carotene, total phosphorus, acid-soluble phosphorus, phospholipids, RNA and DNA of both rye-grasses increased with adequately increasing nitrogen, and reached a maximum with an adequate application of nitrogen. The relationships between yields and crude protein contents, crude protein and RNA contents, and yields and RNA contents of westerworlds and perennial rye-grasses were found to be positively correlated, respectively. Therefore, in general, the response to plant growth by the environmental factors such as nitrogen nutrient may be summarized as follows: Environmental factors\longrightarrowDNA\longrightarrowRNA\longrightarrowProtein\longrightarrowPlant growth

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Study of initial decomposition of the littera of platanus orientalis in stream ecosystem (溪流 生態系에 負荷된 버즘나무 落葉의 初期 分解)

  • Shim, Kue-Cheol;Lee, Sun-Kyung;Lee, Jung-Eun;Chang, Nam-Kee
    • The Korean Journal of Ecology
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    • v.15 no.4
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    • pp.387-394
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    • 1992
  • Decomposition in the litteras of platanus orientalis was studied by month in the dam of seoul national university. total amounts of the litter had the tendency to decrease with the laps of the time. Those of crude protein and phosphate contents, however, increased much more than initial amounts. It was throught that the growth of aquatic microbes gave rise to increase them. Dry weight, organic matter and holocellulose tended to decreased gradually. But, the amount of calcium loss increased rapidly since march and then the amoung of potassium and sodium decreased slowly. It was thought that this changing pattern was ascribed to water temperature, the growth of aquaqtic microbes and the leaching of soluble parts by stream current.

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