• Title/Summary/Keyword: bio-pesticide

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Insecticidal Effect of Neem Cake Extracts on Cabbage Pests, Aphis gossypii and Pluetella xylostella

  • Lee. HoYong;Kim, Won-Rok;Min, Bong Hee
    • Korean Journal of Environmental Biology
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    • v.22 no.4
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    • pp.501-506
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    • 2004
  • In organic agriculture, choose of effective and cheap bio-pesticide is very important. The authors developed an insecticidal extract from neem cake, waste of neem oil from kernel, and applied as a bio-pesticide. Bio-pesticide neem cake extracts experiment on cabbage pest was carried out at Wonju Agricultural Technology and Extension Center from 11 March to 30 May 2003. There were six treatments with three replications, using completely randomized design. Treatments involved three and six sprays of synthetic pyrethroid pesticide cypermethrin 10 EC at the dilution rate of 2.2 mL $L^{-1}$ of distilled water and four, five and six sprays of bio-pesticide neem at the dilution rate of 13.3 mL $L^{-1}$ of distilled water, and untreated control. For each treatment, designated sprayings were done at 7 days interval. Pre-spray data showed that the plants in all the experimental plots were already infested with aphid (Aphis gossypii), and diamondback moth (Pluetella xylostella). The results indicated that all neem pesticide treatments were more effective in insecticidal activity than the untreated control and the chemical treatments in controlling aphids and diamondback moth. Among the three neem treatments, there were no significant differences between them.

Estimating Concentrations of Pesticide Residue in Soil from Pepper Plot Using the GLEAMS Model

  • Jin, So-Hyun;Yoon, Kwang-Sik;Shim, Jae-Han;Choi, Woo-Jung;Choi, Dong-Ho;Kim, Bo-Mi;Lim, Sang-Sun;Jung, Jae-Woon;Lee, Kyoung-Sook;Hong, Su-Myeong
    • Korean Journal of Environmental Agriculture
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    • v.30 no.4
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    • pp.357-366
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    • 2011
  • BACKGROUND: Mathematical model such as GLEAMS have been developed and successfully applied to upland fields to estimate the level of pesticide residues in soil. But, the GLEAMS model rarely applied to the Korean conditions. METHODS AND RESULTS: To evaluate pesticide transport in soil residue using the GLEAMS model from pepper plot, Alachlor, Endosulfan, Cypermethrin and Fenvalerate were applied for standard and double rate. Soil sampling was conducted and decaying patterns of pesticides were investigated. Observed climate data such as temperature and irrigation amount were used for hydrology simulation. The observed pesticide residue data of 2008 were used for parameter calibration, and validation of GLEAMS model was conducted with observed data of 2009. After calibration, the $K_{oc}$ (Organic carbon distribution coefficient) and WSHFRC (Washoff fraction) parameters were identified as key parameters. The simulated concentrations of the pesticides except Fenvalerate were sensitive to $K_{oc}$ parameter. Overall, soil residue concentrations of Alachlor, Cypermethrin and Fenvalerate were fairly simulated compared to those of Endosulfan. The applicability of the GLEAMS model was also confirmed by statistical analysis. CONCLUSION(s): GLEAMS model was eligible for evaluation of pesticide soil residue for Alachlor, Cypermethrin and Fenvalerate.

Establishing and Operating a Test Bench for Assessment of Pesticide Drift by Aerial Application (항공 살포에 의한 농약 비산 측정 및 평가를 위한 시험 농경지 구축 및 운영)

  • Jinseon Park;Se-Yeon Lee;Lak-Yeong Choi;Daniel Kenidh Favour;Se-woon Hong
    • Journal of Bio-Environment Control
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    • v.32 no.4
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    • pp.423-433
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    • 2023
  • As aerial application increasing, with social concerning in pesticide drift rises, so this study attempts to establish a test bench that can repeatedly and continuously evaluate this. To this end, this study first analyze ISO 22866 and ASABE S561.1 among the international standard test methods related to pesticide fugitive evaluation. A test bench was established at the Naju practice field of Chonnam National University in accordance with international standards, and field tests were carried out (ISO 22866, ASABE S561.1) to verify effectiveness. A test bench that established in this study and a pesticide drift recovery protocol by aerial application can improve the experimental environment where field experiments were complex and it was difficult to achieve the same conditions. In addition, it will be possible to construct a database of pesticide drift that takes into account various factors that affect pesticide drift substances, which is expected to improve the reliability of the data, as well as quantitative evaluation of pesticide drift in the air.

Development of Colorimetric Paper Sensor for Pesticide Detection Using Competitive-inhibiting Reaction

  • Kim, Hyeok Jung;Kim, Yeji;Park, Su Jung;Kwon, Chanho;Noh, Hyeran
    • BioChip Journal
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    • v.12 no.4
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    • pp.326-331
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    • 2018
  • Contamination by pesticides is an everincreasing problem associated with fields of environmental management and healthcare. Accordingly, appropriate treatments are in demand. Pesticide detection methods have been researched extensively, aimed at making the detection convenient, fast, cost-effective, and easy to use. Among the various detecting strategies, paper-based assay is potent for real-time pesticide sensing due to its unique advantages including disposability, light weight, and low cost. In this study, a paper-based sensor for chlorpyrifos, an organophosphate pesticide, has been developed by layering three sheets of patterned plates. In colorimetric quantification of pesticides, the blue color produced by the interaction between acetylcholinesterase and indoxyl acetate is inhibited by the pesticide molecules present in the sample solutions. With the optimized paper-based sensor, the pesticide is sensitively detected (limit of detection =8.60 ppm) within 5min. Furthermore, the shelf life of the device is enhanced to 14 days after from the fabrication, by treating trehalose solution onto the deposited reagents. We expect the paper-based device to be utilized as a first-screening analytic device for water quality monitoring and food analysis.

Analysis of Pesticides in Herbal Medicine by QuEChERS and GC-MS/MS (QuEChERS 전처리와 GC-MS/MS를 활용한 생약의 잔류농약 분석 가능성 연구)

  • Seo, Go Eun;Kim, A Young;Pyo, Byoung Sik;Lee, Kyoung in
    • Korean Journal of Pharmacognosy
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    • v.51 no.3
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    • pp.207-216
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    • 2020
  • The analysis method for qualitative analysis of the screening method that can be performed prior to the quantitative analysis of individual pesticide was reviewed in order to meet the safety standards that are being strengthened in the field of pesticide residue testing of herbal medicines. Among the residual pesticides presented in the Korean Pharmacopoeia, 56 pesticides, excluding 15 pesticides that need to be individually analyzed, were selected for analysis using QuEChERS preprocessing and GC-MS/MS, which are used in the existing agricultural products field. For each pesticide, the detection limit level of 0.001-0.005 mg/kg and the quantitative limit level of 0.002-0.017 mg/kg were confirmed. In the recovery test in which the standard was treated at a concentration of 0.02 mg/kg, it was confirmed that the proportion of pesticides satisfying the recovery of 70-120% was 85.7-96.4% for each herbal medicine, so it was confirmed that it was a level that could be reviewed by the screening method.

Synthesis and fungicidal activity of new ${\beta}$-methoxyacrylate derivatives having thio-enol side chain (티오엔을 곁가지를 가진 메톡시아크릴레이트 화합물의 합성 및 살균활성 연구)

  • Lee, Hyeon-Kyu;Kim, Ji-A;Choi, Eun-Bok;Park, Chwang-Siek;Choi, Gyung-Ja
    • The Korean Journal of Pesticide Science
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    • v.9 no.2
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    • pp.132-139
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    • 2005
  • New ${\beta}$-methoxyacrylate derivatives 1-4 having thio-enol side chain were prepared and subjected to in vivo screening for fungicidal activity against phytopathogenic fungi and many of them showed good fungicidal activities against especially rice blast and wheat leaf rust at 100 ppm.

Enhanced Biodegradation of Lindane Using Oil-in-Water Bio-Microemulsion Stabilized by Biosurfactant Produced by a New Yeast Strain, Pseudozyma VITJzN01

  • Abdul Salam, Jaseetha;Das, Nilanjana
    • Journal of Microbiology and Biotechnology
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    • v.23 no.11
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    • pp.1598-1609
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    • 2013
  • Organochlorine pesticide residues continue to remain as a major environmental threat worldwide. Lindane is an organochlorine pesticide widely used as an acaricide in medicine and agriculture. In the present study, a new lindane-degrading yeast strain, Pseudozyma VITJzN01, was identified as a copious producer of glycolipid biosurfactant. The glycolipid structure and type were elucidated by FTIR, NMR spectroscopy, and GC-MS analysis. The surface activity and stability of the glycolipid was analyzed. The glycolipids, characterized as mannosylerythritol lipids (MELs), exhibited excellent surface active properties and the surface tension of water was reduced to 29 mN/m. The glycolipid was stable over a wide range of pH, temperature, and salinity, showing a very low CMC of 25 mg/l. Bio-microemulsion of olive oil-in-water (O/W) was prepared using the purified biosurfactant without addition of any synthetic cosurfactants, for lindane solubilization and enhanced degradation assay in liquid and soil slurry. The O/W bio-microemulsions enhanced the solubility of lindane up to 40-folds. Degradation of lindane (700 mg/l) by VITJzN01 in liquid medium amended with bio-microemulsions was found to be enhanced by 36% in 2 days, compared with degradation in 12 days in the absence of bio-microemulsions. Lindane-spiked soil slurry incubated with bio-microemulsions also showed 20-40% enhanced degradation compared with the treatment with glycolipids or yeast alone. This is the first report on lindane degradation by Pseudozyma sp., and application of bio-microemulsions for enhanced lindane degradation. MEL-stabilized bio-microemulsions can serve as a potential tool for enhanced remediation of diverse lindane-contaminated environments.

A Study on the Development of High Density Ozone Water Cleaning System for Herb Medicine (고농도 오존수를 이용한 한약재 세척기기에 대한 연구)

  • Kim, Jeong-Ho;Bang, Byeong-Hun;Lee, Sang-Hwa;Kim, Sung-Hee
    • Journal of Acupuncture Research
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    • v.29 no.2
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    • pp.9-14
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    • 2012
  • Objectives : This study was designed to evaluate the pesticide residue of herb medicine through high density ozone water cleaning system. Materials and Methods : We purchased ginseng(Panax ginseng) on market and sprayed pesticides (Diazinon) on ginseng. We analyzed pesticide residue according to washing methods(untreated, ozone water cleaning, ultrasonic cleaning, Water cleaning). As a result of each washing methods, in ozone water cleaning method pesticide residue was much less remained than in Ultrasonic Cleaning method and water cleaning method. Conclusions : High density ozone water cleaning is the best method for removing pesticides of herbal medicine.

Synthesis and Herbicidal Activities of Hydantoin Derivatives Possessing Amide Subgroup (아미드 치환체를 갖는 히단토인계 화합물의 합성과 제초활성 연구)

  • Ko, Young-Kwan;Chung, Keun-Hoe;Ryu, Jae-Wook;Woo, Jae-Chun;Koo, Dong-Wan;Choi, Jung-Sub;Kim, Jun-Young;Kim, Tae-Joon;Kwon, Oh-Yeon;Chung, Bong-Jin;Kim, Dae-Whang
    • The Korean Journal of Pesticide Science
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    • v.10 no.2
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    • pp.153-156
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    • 2006
  • As an ongoing research program for the development of environmentally friendly new herbicide, several hydantoin derivatives 5a - 5i possessing amide subgroup were synthesized and shown to have interesting herbicidal activities exhibiting symptoms as Protoporphyrinogen IX oxidase inhibitor under postemergence upland greenhouse screening. Among derivatives tested, compound 5h showed superior herbicidal activity against upland problem weed, digitaria sanguinalis and aeschynomene indica to reference compound fluthiacet-methyl.

Synthesis and Herbicidal Activities of Tetrahydroindazole Derivatives Possessing Hydroxyalkyl Subgroup (하이드록시알킬 치환체를 갖는 테트라하이드로인다졸계 화합물의 합성과 제초활성 연구)

  • Ko, Young-Kwan;Chung, Kun-Hoe;Ryu, Jae-Wook;Woo, Jae-Chun;Koo, Dong-Wan;Choi, Jung-Sup;Kim, Jun-Young;Kim, Su-Ho;Kim, Tae-Joon;Choi, In-Young;Seok, Mee-Young;Choi, Jun-Hyuk;Chung, Bong-Jin
    • The Korean Journal of Pesticide Science
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    • v.12 no.2
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    • pp.201-205
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    • 2008
  • As an ongoing research program for the development of environmental friendly new soil-surface treatment herbicide, several tetrahydroindazole derivatives 6a - 6h possessing hydroxyalkyl subgroup were synthesized and shown to have interesting herbicidal activities exhibiting sumptoms as protoporphyrinogen IX oxidase inhibitor under pre-emergence upland greenhouse screening. Among derivatives tested, compound 6f showed superior herbicidal activity against problem weeds.