BioChip Journal

The Korean BioChip Society (한국바이오칩학회)
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Development of Colorimetric Paper Sensor for Pesticide Detection Using Competitive-inhibiting Reaction

  • Kim, Hyeok Jung (Department of Optometry, Seoul National University of Science and Technology (Seoultech)) ;
  • Kim, Yeji (Department of Optometry, Seoul National University of Science and Technology (Seoultech)) ;
  • Park, Su Jung (Department of Optometry, Seoul National University of Science and Technology (Seoultech)) ;
  • Kwon, Chanho (Research Institute, Biomax Co., Ltd.) ;
  • Noh, Hyeran (Department of Optometry, Seoul National University of Science and Technology (Seoultech))
  • Received : 2018.04.16
  • Accepted : 2018.06.25
  • Published : 2018.12.20
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Abstract

Contamination by pesticides is an everincreasing problem associated with fields of environmental management and healthcare. Accordingly, appropriate treatments are in demand. Pesticide detection methods have been researched extensively, aimed at making the detection convenient, fast, cost-effective, and easy to use. Among the various detecting strategies, paper-based assay is potent for real-time pesticide sensing due to its unique advantages including disposability, light weight, and low cost. In this study, a paper-based sensor for chlorpyrifos, an organophosphate pesticide, has been developed by layering three sheets of patterned plates. In colorimetric quantification of pesticides, the blue color produced by the interaction between acetylcholinesterase and indoxyl acetate is inhibited by the pesticide molecules present in the sample solutions. With the optimized paper-based sensor, the pesticide is sensitively detected (limit of detection =8.60 ppm) within 5min. Furthermore, the shelf life of the device is enhanced to 14 days after from the fabrication, by treating trehalose solution onto the deposited reagents. We expect the paper-based device to be utilized as a first-screening analytic device for water quality monitoring and food analysis.

Keywords

Acknowledgement

Supported by : Ministry of SMEs and Startups(MSS)

References

  1. Schreinemachers, P. & Tipraqsa, P. Agricultural pesticides and land use intensification in high, middle and low income countries. Food Policy 37, 616-626 (2012). https://doi.org/10.1016/j.foodpol.2012.06.003
  2. Popp, J., Peto, K. & Nagy, J. Pesticide productivity and food security: a Review. Agron. Sustainable Dev. 33, 243-255 (2013). https://doi.org/10.1007/s13593-012-0105-x
  3. Jeyaratnam, J. Health problems of pesticide usage in the Third World. Br. J. Ind. Med. 42, 505-506 (1985).
  4. Ecobichon, D.J. Pesticide use in developing countries. Toxicology 160, 27-33 (2001). https://doi.org/10.1016/S0300-483X(00)00452-2
  5. Eddleston, M. et al. Pesticide poisoning in the developing world - a minimum pesticides list. The Lancet 360, 1163-1167 (2002). https://doi.org/10.1016/S0140-6736(02)11204-9
  6. Martinez Andres, W., Phillips Scott, T., Butte Manish, J. & Whitesides George, M. Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew. Chem. Int. Ed. 46, 1318-1320 (2007). https://doi.org/10.1002/anie.200603817
  7. Chen, G.-H. et al. Detection of mercury (II) ions using colorimetric gold nanoparticles on paper-based analytical devices. Anal. Chem. 86, 6843-6849 (2014). https://doi.org/10.1021/ac5008688
  8. Jokerst, J.C. et al. Development of a paper-based analytical device for colorimetric detection of select foodborne pathogens. Anal. Chem. 84, 2900-2907 (2012). https://doi.org/10.1021/ac203466y
  9. Jayawardane, B.M., Wei, S., McKelvie, I.D. & Kolev, S.D. Microfluidic paper-based analytical device for the determination of nitrite and nitrate. Anal. Chem. 86, 7274-7279 (2014). https://doi.org/10.1021/ac5013249
  10. Peters, K.L. et al. Simultaneous colorimetric detection of improvised explosive compounds using microfluidic paper-based analytical devices (${\mu}PADs$). Anal. Methods 7, 63-70 (2015). https://doi.org/10.1039/C4AY01677G
  11. Wu, Y., Sun, Y., Xiao, F., Wu, Z. & Yu, R. Sensitive inkjet printing paper-based colormetric strips for acetylcholinesterase inhibitors with indoxyl acetate substrate. Talanta 162, 174-179 (2017). https://doi.org/10.1016/j.talanta.2016.10.011
  12. Hossain, S.M.Z., Luckham, R.E., McFadden, M.J. & Brennan, J.D. Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. Anal. Chem. 81, 9055-9064 (2009). https://doi.org/10.1021/ac901714h
  13. Hossain, S.M.Z. et al. Development of a bioactive paper sensor for detection of neurotoxins using piezoelectric inkjet printing of sol-gel-derived bioinks. Anal. Chem. 81, 5474-5483 (2009). https://doi.org/10.1021/ac900660p
  14. Badawy, M.E. & El-Aswad, A.F. Bioactive paper sensor based on the acetylcholinesterase for the rapid detection of organophosphate and carbamate pesticides. Int. J. Anal. Chem. http://dx.doi.org/10.1155/2014/536823 (2014).
  15. Jahanshahi-Anbuhi, S. et al. Creating fast flow channels in paper fluidic devices to control timing of sequential reactions. Lab Chip 12, 5079-5085 (2012). https://doi.org/10.1039/c2lc41005b
  16. Nouanthavong, S., Nacapricha, D., Henry, C.S. & Sameenoi, Y. Pesticide analysis using nanoceria-coated paper-based devices as a detection platform. Analyst 141, 1837-1846 (2016). https://doi.org/10.1039/C5AN02403J
  17. Kavruk, M., Ozalp, V.C. & Oktem, H.A. Portable bioactive paper-based sensor for quantification of pesticides. J. Anal. Methods Chem. http://dx.doi.org/10.1155/2013/932946 (2013).
  18. Chang, J., Li, H., Hou, T. & Li, F. Paper-based fluorescent sensor for rapid naked-eye detection of acetylcholinesterase activity and organophosphorus pesticides with high sensitivity and selectivity. Biosens. Bioelectron. 86, 971-977 (2016). https://doi.org/10.1016/j.bios.2016.07.022
  19. Cotson, S. & Holt, S.J. IV. Kinetics of aerial oxidation of indoxyl and some of its halogen derivatives. Proc. R. Soc. Lond. B. Biol. Sci. 148, 506 (1958). https://doi.org/10.1098/rspb.1958.0042
  20. Pohanka, M., Hrabinova, M., Kuca, K. & Simonato, J.-P. Assessment of acetylcholinesterase activity using indoxylacetate and comparison with the standard Ellman's method. Int. J. Mol. Sci. 12, 2631-2640 (2011). https://doi.org/10.3390/ijms12042631
  21. Pesticides Database Search: 17 - Chlorpyrifos. www.fao.org/fao-who-codexalimentarius/codex-texts/dbs/pestres/pesticide-detail/en/?p_id=17 (2013).
  22. Han, Y. et al. Effects of sugar additives on protein stability of recombinant human serum albumin during lyophilization and storage. Arch. Pharmacal Res. 30, 1124 (2007). https://doi.org/10.1007/BF02980247

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