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http://dx.doi.org/10.1007/s13206-018-2404-z

Development of Colorimetric Paper Sensor for Pesticide Detection Using Competitive-inhibiting Reaction  

Kim, Hyeok Jung (Department of Optometry, Seoul National University of Science and Technology (Seoultech))
Kim, Yeji (Department of Optometry, Seoul National University of Science and Technology (Seoultech))
Park, Su Jung (Department of Optometry, Seoul National University of Science and Technology (Seoultech))
Kwon, Chanho (Research Institute, Biomax Co., Ltd.)
Noh, Hyeran (Department of Optometry, Seoul National University of Science and Technology (Seoultech))
Publication Information
BioChip Journal / v.12, no.4, 2018 , pp. 326-331 More about this Journal
Abstract
Contamination by pesticides is an everincreasing problem associated with fields of environmental management and healthcare. Accordingly, appropriate treatments are in demand. Pesticide detection methods have been researched extensively, aimed at making the detection convenient, fast, cost-effective, and easy to use. Among the various detecting strategies, paper-based assay is potent for real-time pesticide sensing due to its unique advantages including disposability, light weight, and low cost. In this study, a paper-based sensor for chlorpyrifos, an organophosphate pesticide, has been developed by layering three sheets of patterned plates. In colorimetric quantification of pesticides, the blue color produced by the interaction between acetylcholinesterase and indoxyl acetate is inhibited by the pesticide molecules present in the sample solutions. With the optimized paper-based sensor, the pesticide is sensitively detected (limit of detection =8.60 ppm) within 5min. Furthermore, the shelf life of the device is enhanced to 14 days after from the fabrication, by treating trehalose solution onto the deposited reagents. We expect the paper-based device to be utilized as a first-screening analytic device for water quality monitoring and food analysis.
Keywords
Pesticide; Paper microfluidic; Colorimetric sensing; Three-dimensional paper device;
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