• Title/Summary/Keyword: bi-level

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Studies on the Induction of Transformation and Multiplication in Orchid Plants.(III) Expression of Gene Transferred into Orchid Protoplasts by Electroporation (난과식물의 형질전환 유도 및 다량증식에 관한 연구. III. Electroporation에 의해서 자란의 원형질체로 도입된 유전자의 발현)

  • 이정석;황성진김영준황백
    • KSBB Journal
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    • v.6 no.4
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    • pp.385-388
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    • 1991
  • Embryogenic cell suspension cultures of B. striata were established as transferred selected embryogenic callus into liquid medium. Protoplasts isolated from embryogenic cell suspensions were electroporated in buffered solutions containing plasmid DNA of pBI121. Transient GUS (beta-glucuronidase) activity measurement and selection for kanamycin resistent showed that expression of foreign genes and stable transformation were achieved. GUS transient gene expression was increased by increasing DNA concentration of pBI121 plasmid and affected by the level of the applied voltage. An optimal level of GUS activity was obtained after electroporation with a pulse of 200-300 voltage/1180 uF. Protoplast viability was up to the 80% at the optimal voltage.

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A Comparative Study on Country of Origin Discrepancy and Brand Authenticity (원산지정보 불일치와 브랜드 진정성에 관한 비교연구)

  • Inwon Kang;Shanshan Liang
    • Korea Trade Review
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    • v.47 no.4
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    • pp.161-176
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    • 2022
  • Most companies are achieving economic benefits by producing products in the form of bi-national products, but there are also negative consequences such as reduced brand power, so strategic bi-national product planning and branding is essential. In order to actively reflect these practical needs, this study conducted a comprehensive and easy-to-generalize evaluation of Bi-national products that has been somewhat underdeveloped in previous studies. To this end, the selection and evaluation criteria of the products to be investigated were differentiated. This is to classify the products by place of use and the purpose of use so that the majority of bi-national products can be included, and then use the combination to derive the specific products to be evaluated for bi-national products. The evaluation of the product identified the phenomenon of psychological conflict among consumers due to the country of origin discrepancy at the level of decreasing brand authenticity. The above research design is an evaluation method that can encompass bi-national products as a whole, rather than an evaluation of a specific product or product line that is mainly reported in previous studies, and it is also very easy to apply to practice because it allows us to more carefully identify changes in psychological attitudes.

Dynamic Origin-Destination Demand Estimation Using Traffic Data of VDS and AVI (VDS 및 AVI 자료를 이용한 고속도로 동적OD 추정)

  • Kim, Ju-Young;Lee, Seung-Jae;Lee, Young-Ihn;Son, Bong-Soo
    • Journal of Korean Society of Transportation
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    • v.23 no.7 s.85
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    • pp.125-136
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    • 2005
  • The goal of this paper is to develop freeway Origin-Destination (OD) demand estimation model using VDS and AVI data. The formulation of methodology proposed in this paper includes traffic flow technique to be able to remove the bi-level problem and optimal solution algorithm using a kalman filter algorithm. The proposed dynamic OD estimation model use ilk and off-ramp volumes collected from VDS and partial OD collected from AVI data to raise the accuracy of dynamic OD estimation. The proposed model is evaluated by using the real-time data of SOHAEAN freeway, South Korea. The result of the proposed dynamic OD estimation model based on VDS and AVI data is better than that of based on VDS data. The more AVI systems are equipped at on and off-ramp, the more excellent result of estimation accuracy is expected.

Diffraction Characteristics for Optical Bio-Sensor of Bi-level Grating with Mushroom Profile (버섯형 이중 격자구조의 광 바이오센서에 대한 회절 특성)

  • Ho, Kwang-Chun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.22 no.2
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    • pp.129-134
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    • 2022
  • The resonant bio-sensor of bi-level grating structure with mushroom profile has been designed for operating in the near-infrared (NIR) wavelength range under transverse electric (TE) polarization. The rigorous modal transmission-line theory (MTLT) is applied to determine the optical characteristics, and the reflection resonance of the grating structure is analyzed by varying their geometrical parameters. The numerical result shows that an excited sharp Fano resonance (FR), which does not occur in single layer grating, is demonstrated. The relationship between structure parameters of bi-level grating and the reflectance spectrum in order to guarantee the appearance of FR in the designed structure is fully investigated. An optical bio-sensor with a potential sensitivity of 112.9~214.3 deg/RIU and 447 nm/RIU is designed based on the proposed structure. The proposed mushroom profile may serve as a powerful sample for the design of optical bio-sensors with a wide range of applications.

Antioxidant Activities of Methanol Extracts from Root Parts of Korean Salad Plants

  • Chon, Sang-Uk;Kim, Tae-Soon;Shin, Ji-San;Boo, Hee-Ock
    • Korean Journal of Plant Resources
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    • v.21 no.6
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    • pp.413-419
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    • 2008
  • Phenolics level, total flavonoid content, and antioxidant were determined from the methanol extracts of the eight medicinal plants using roots. Total phenolics were found as the highest levels in the methanol extracts of Arctium lappa, and followed by Youngia sonchifolia and Cirsium japonicum. Total amount of the each phenol compounds were detected in C. japonicum extracts ($319.2\;mg\;kg^{-1}$) as the greatest component, and followed by A. lappa ($96.3\;mg\;kg^{-1}$) and Y. sonchifolia ($22.9\;mg\;kg^{-1}$). Total flavonoid content showed the highest amount in methanol extracts from A. lappa (68.1 mg $100\;g^{-1}$) and followed by Y. sonchifolia (11.2 mg $100\;g^{-1}$) Lycoris radiate extracts (87.2%) had the highest nitrite scavenging activity and followed by A. lappa (81.5%) and Y. sonchifolia (77.5%). Methanol extracts of A. lappa at 25 mg $100\;g^{-1}$ exhibited the highest DPPH radical scavenging activity by 90.8%, even though less activity than synthetic antioxidants Vitamin C or butylated hydroxytoluene (BHT). Level of polyphenols was highly correlated with anti oxidative activity ($r^2$ = 0.85). The results suggest that several medicinal plants selected had the potent biological activities, and that their activities were differential depending on plant species.

The Effect of Physical Therapy on Functional Change and Related Factors in Stroke Patients (뇌졸중환자의 물리치료경과에 따른 기능변화와 관련요인)

  • Lee Seung-Ju;Yeh Min-Hae;Chun Byung-Yeol
    • The Journal of Korean Physical Therapy
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    • v.10 no.1
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    • pp.7-21
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    • 1998
  • An analysis of 101 stroke patients who were enrolled in 10 hospitals of Cities of Pusan, Taegu, and Andong from November 1, 1996 to April 31. 3997 was conducted using the modified Barthel Index(BI) and the adapted PULSES profile index(PS) to evaluate their function. Patients were examined at the following intervals: Initial assessment, one month after initial. at discharge, and one month after discharge. The mean BI score of patients initial assessment was 27.18, and that of PS was 17.54. There were statistically significant between initial score and one month after initial (21.39: p<0.001), at discharge(37.47: p<0.001), one month after discharge(46.49: p<0.001). PS scores were also improved .significantly(-2.62, -4.52. and -6.26(p<0.001). And the score between dischange and one month after discharge was significant (9.01: p<0.001) and in PS score(-1.73: p<0.001). Age and BE score were significantly associated with the improved in BI score between initial and discharge(T3-T1)(p<0.05). Below age forty the Bower initial BI score showed significantly higher improvement(T3-T1) after physical therapy(p<0.05). Initial Bl score, patients' attitude for physical therapy after discharge, age, and surgical operation were significantly associated with the improvement of BI score between initial and one month after discharge(T4-T1)(p<0.05). The lower initial BI score, patients' positive attitude for physical therapy after discharge, below age forty. and no surgical operation showed significantly higher improvement(p<0.05). BI score at discharge, side of hemiparesis and religion were significantly associated with the improvement of BI score between at discharge and one month after discharge(T4-T3) (p<0.05). The lower BI score at discharge. left aide of hemiparesis, with religion showed significantly higher improvement (p<0.05). Age, initial PS score were significantly associated with the improved in PS score between initial and discharge(T3-T1)(p<0.05). The higher initial PS score and below age forty showed significantly higher improvement(T3-T1)(p<0.05). Initial PS score, patient' attitude for physical therapy after discharge, age, educational level, physical therapy hour after discharge, and surgical operation were significantly associated with the improvement of PS score between initial and one month after discharge(T4-T1)(p<0.05). The higher initial PS score, patients' positive attitude for physical therapy after discharge, below age forty, higher education, the shorter physical therapy hour, and no surgical operation showed significantly higher improvement(T4-T1)(p<0.05). PS score at discharge, educational level, patient' attitude far physical therapy after discharge, physical therapy hour after discharge, and gender were significantly associated with the improvement of PS score between discharge and une month after discharge(T4-T3) (p<0.05), The higher PS non at discharge, higher education, patients' positive attitude for Physical therapy after discharge, the shorter physical therapy hour, and male showed significantly higher improvement (T4-T3)(p<0.05). In conclusion, initial BI score and age were significantly associated with BI score improvement and initial PS score, age, and educational level were also significantly associated with PS score improvement in stoke patients.

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Grain Boundary Trap Levels in ZnO-based Varistor (ZnO계 바리스터의 입계포획준위)

  • Kim, Myung-Chul;Park, Soon-Ja
    • Korean Journal of Materials Research
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    • v.2 no.1
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    • pp.12-18
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    • 1992
  • The trap levels of ZnO-based varistor are obtained by Isothermal Capacitance Transient Spectroscopy method. Here ICTS measuring system consists of YHP 4192A Impedance Analyzer and a personal computer for the data acquisition. Between $-40^{\circ}C$ and $60^{\circ}C$, the grain boundary trap levels of 0.48 and 0.94eV were detected for $ZnO-Bi_2O_3-MnO$ system. The hole omission spectra are observed in the case of the addition of CoO into the $ZnO-Bi_2O_3$ system, while the electron emission spectra are detected in the case of the addition of MnO. The nonlinear resistance coefficient $\alpha$ increases with the decrease of the dormer concentration. Finally, the trap level density of $ZnO-Bi_2O_3-MnO$ system is found to decrease with the amount of CoO, while $\alpha$ is found to increase with the amount of CoO.

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Bi-Directional Multi-Level Converter for an Energy Storage System

  • Han, Sang-Hyup;Kim, Heung-Geun;Cha, Honnyong;Chun, Tae-Won;Nho, Eui-Cheol
    • Journal of Power Electronics
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    • v.14 no.3
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    • pp.499-506
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    • 2014
  • This paper proposes a 3 kW single-phase bi-directional multi-level converter for energy storage applications. The proposed topology is based on the H-bridge structure with four switches connected to the DC-link. A simple phase opposition disposition PWM method that requires only one carrier signal is also suggested. The switching sequence to balance the capacitor voltage is considered. The topology can be extended to a nine-level converter or a three-phase system. The operating principle of the proposed converter is verified through a simulation and an experiment.

High Efficiency H-Bridge Multilevel Inverter System Using Bidirectional Switches (양방향 스위치를 이용한 고효율 H-Bridge 멀티레벨 인버터 시스템)

  • Lee, Hwa-Chun;Hwang, Jung-Goo;Kim, Sun-Pil;Choi, Woo-Seok;Lee, Sang-Hyeok;Park, Seong-Mi;Park, Sung-Jun
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.28 no.10
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    • pp.16-26
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    • 2014
  • This paper proposes new 13-level inverter topology and DC/DC converter buck-boost structure topology for multilevel, compounding uni-directional and bi-directional switches, and proposes high-efficient multilevel inverter system in which the proposed two PCS(Power Conditioning System) was connected in series. In proposed multilevel inverter of forming a output 13-level phase voltage by using total 18 switching parts, Then bi-directional switch has a characteristic of reducing conduction loss and controlling the reactive power effectively by separating electrically from the neutral point. DC/DC converter for supplying in dependent 3 DC voltage to the proposed multi-level inverter generates 180-degree phase shifted PWM by the symmetrically combined structure of 2 buck-boost converter and twice switching frequency efficiency can be obtained, meanwhile, the converter can step up/down the output voltage and 20% output can be generated comparing the input voltage. This proposed system is verified with the simulation and laboratory test.

Strategy for Providing Optimal VMS Travel Time Information Using Bi-Level Programming (Bi-Level 프로그래밍 기법을 이용한 최적의 VMS 통행시간 정보제공 전략)

  • Baik, Nam Cheol;Kim, Byung Kwan;Lee, Sang Hyup
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.4D
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    • pp.559-564
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    • 2006
  • The purpose of this study is to minimize negative effect of VMS travel time information service by sensitivity analysis, which forecasts the change in link traffic volume. As a result, strategies for providing travel information that can change driving patterns for minimizing travel time were found. The framework for analysis is recently expanded with the application of game theory. According to the experiment, the algorithm generated for travel time information service reduces total travel time and yields travel patterns that is very close to the system optimization. Also, this study found that the route the travel time service information is provided about could play the important role.