• Journal of Periodontal and Implant Science
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• 제30권4호
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• pp.793-803
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• 2000

Procedures for treatment of molar furcation invasion defects range from open flap debridement, apically repositioned flap surgery, hemisection, tunneling or extraction, to regenerative therapies using bone grafting or guided tissue regenerative therapy, or a combination of both. Several clinical evaluations using regenerative techniques have reported the potential for osseous repair of treated furcation invasions. Regenerative treatment of maxillary molars are more difficult due to the multiple root anatomy and multiple furcation entrances therefore, purpose of this study was to evaluated histologically self-curing glass-ionomer cement and light-curing glass-ionomer cement as a barrier in the treatment of a bi-furcated maxillary premolar. Five adult beagle dogs were used in this experiment. With intrasulcular and crestal incision, mucoperiosteal flap was elevated. Following decortication with 1/2 high speed round bur, degree II furcation defect was made on maxillary third(P3), forth(P4) and fifth(P5) premolar. 2 month later experimental group were self-curing glassionomer cement and light-curing glassionomer cement. After 4, 8 weeks, the animals were sacrificed by vascular perfusion. Tissue block was excised including the tooth and prepared for light microscope with Gomori's trichrome staining. Results were as follows. 1. In all experiment group, there were not epithelial down growth and glass ionomer cement were encapsulated connective tissue. 2. In 4 weeks experiment I group slighly infiltrated inflammatory cells but not disturb the new bone or new cementum formation. 3. In 8 weeks, experiment groups I, II were encapsulated fine connective tissue. 4. Therefore glass-ionomer cement filling to the grade III maxillary furcations with multiple root anatomy and multiple furcation entrances were possible clinical methods and this technique is useful method for Maxillary furcation involvement.

• Journal of Periodontal and Implant Science
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• 제30권4호
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• pp.803-814
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• 2000

Current acceptable methods of promoting periodontal regeneration are basis of removal of diseased soft tissue, root treatment, guided tissue regeneration, graft materials, biological mediators. Platelet Rich Plasma have been reported as a biological mediator which regulate activities of wound healing progress including cell proliferation, migration, and metabolism. The purpose of this study is to evaluate the possibility of using the Platelet Rich Plasma as a regeneration promoting agent for furcation involvement defect. Five adult beagle dogs were used in this experiment. With intrasulcular and crestal incision, mucoperiosteal flap was elevated. Following decortication with 1/2 high speed round bur, degree II furcation defect was made on mandibular third(P3), forth(P4) and fifth(P5) premolar. 2 month later experimental group were PRP plus bovine bone and bovine bone only. After 4, 8 weeks, the animals were sacrificed by perfusion technique. Tissue block was excised including the tooth and prepared for light microscope with Gomori's trichrome staining. At 4 weeks after surgery, there were rapid osteogenesis phenomenon on the defected area of the Platelet Rich Plasma plus bovine bone group and early trabeculation pattern was made with new osteoid tissue produced by activated osteoblast. Bone formation was almost completed to the fornix of furcation by 4 weeks after surgery. In conclusion, Platelet Rich Plasma can promote rapid osteogenesis during early stage of periodontal tissue regeneration.

• Journal of Periodontal and Implant Science
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• 제27권3호
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• pp.633-645
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• 1997

The recent trend of research and development on guided tissue regeneration focuses on the biodegradable membranes, which eliminate the need for subsequent surgical removal. They have demonstrated significant and equivalent clinical improvements to the ePTFE membranes. This study evaluate guided tissue regeneration wound healing in surgically induced intrabony periodontal defects following surgical treatment with a synthetic biodegradable membranes, made from a copolymer of glycolide and lactide, in 8 beagle dogs. After full thickeness flap reflection, exposed buccal bone of maxillary and mandibular canine and premolar was removed surgically mesiodistally and occlusoapically at $6mm{\times}6mm$ in size for preparation of periodontal defects. In experimental sites a customized barrier was formed and fitted to cover the defect. Flap was replaced slightly coronal to CEJ and sutured. Plaque control program was initiated and maintained until completion of the study. In 4, 8, 16 and 24 weeks after surgery, the animals were sacrificed and then undecalcified specimens were prepared for histologic evaluation. Histologic examination indicated significant periodontal regeneration characterized by new connective tissue attachment, cementum formation and bone formation. These membranes showed good biocompatibility throughout experiodontal period. The barriers had been completely resorbed with no apparent adverse effect on periodontal wound healing at 24 weeks. These results implicated that present synthetic biodegradable membrane facilitated guided tissue regeneration in periodontal defect.

• 한국동물생명공학회지
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• 제38권1호
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• pp.38-46
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• 2023

In this study, an experiment was conducted in order to determine what cryopreservatives (CPVs) were more effective in supporting the motility and viability of sperm from experimental animals. The sperm of mice, rats, beagle dogs, and rabbits were frozen using different CPVs, including DMSO, TYB, and Sperm CryoProtec. The results from freezing the sperm of each laboratory animal in Sperm CryoProtec showed a high level of sperm motility and viability in sperm samples from mice, rats, and beagle dogs melted at the end of the first week. For rabbits, a high level of motility was observed in sperm thawed during the first week, whereas a high level of viability was observed in sperm thawed during the second week. The results of analysis of sperm motility and viability using different CPVs according to laboratory animals showed a significantly higher level of sperm motility (26.28%) and viability (36.20%) for mice in Sperm CryoProtec and the lowest levels of motility and viability were observed in DMSO (p < 0.05). Significantly higher levels of motility (27.94%) and viability (37.94%) were observed for rats in Sperm CryoProtec compared with TYB, which showed the lowest levels of motility and viability (p < 0.05). The study findings described above suggest that the selection of appropriate cryopreservatives is required for each experimental animal. This is because there are differences in the levels of sperm motility and viability of experimental animals depending on the CPVs that are typically used for freezing human sperm, including Sperm CryoProtec and TYB.

• 한국임상수의학회지
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• 제32권5호
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• pp.422-425
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• 2015

이번 연구는 비글 개에서 medetomidine(D), medetomidine, tiletamine/zolazepam(DZ) 합제, medetomidine, tiletamine/zolazepam, tramadol(DZT) 합제를 이용한 진정/마취 후 심근 기능을 평가하였다. 10 마리의 건강한 성견 비글(체중 $8.6{\pm}1.0$ kg)를 이번 연구에 사용하였다. M-mode 심장초음파를 사용하여 심박수(HR), 구획단축률(%FS), 좌심실 박출기계수(%LVEF), 박출량(SV), 심박출량(CO), 수축기 좌심실 내강 직경 (LVIDs), 이완기 좌심실 내강 직경(LVIDd)을 마취 전, 그리고 마취 후 10분 간격으로 60분 동안 측정하였다. HR, %FS, %LVEF, SV, CO 는 D, DZ 합제, DZT 합제 로 진정된 동안 심각하게 감소하였다. 이번 실험에 사용된 마취 프로토콜은 사용 가능할 정도의 진정/마취가 이뤄졌지만, 심혈관계 압박의 정도가 심했고 지속적이었고, 따라서, 어떠한 상황에서도 생체지수에 대한 모니터링이 동반되어야 한다. 본 마취 프로토콜은 심장질환을 가지고 있어서 심맥관계 억압의 가능성이 높은 환자에서는 주의해서 사용해야 한다.

• 한국임상수의학회지
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• 제28권6호
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• pp.591-594
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• 2011

9개월령의 암컷 말티즈견과 1년령의 암컷 비글견이 지속성 심잡음을 주증으로 내원 하였다. 두 환축은 2차원 심초음파 및 심혈관조영술을 통해 동맥관개존증 (PDA)으로 진단되었다. 평가된 환축들의 동맥관 사이즈는 비교적 큰 편이었으며, 혈관 폐색장치 중 Amplatzer$^{(R)}$ vascular plug를 통한 동맥관의 폐쇄술이 시도 되었다. 말티즈 견의 경우, Amplatzer$^{(R)}$ vascular plug를 이용하여 동맥관의 완전폐쇄가 이루어 졌으며, 폐쇄직후 지속성 심잡음이 사라졌다. 비글견의 경우, Amplatzer$^{(R)}$ vascular plug 삽입 후 심잡음이 줄어들긴 했지만, 심혈관 조영검사에서 잔류혈류가 관찰되었다. 시술의 안전성 및 시술 후 합병증은 두 환자 모두에서 지속적으로 모니터링 되었다. 본 2 증례는, Amplatzer$^{(R)}$ vascular plug를 이용한 성공적인 동맥관개존증 폐쇄술에 대한 국내 첫 증례보고이다.

• 한국임상수의학회지
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• 제29권6호
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• pp.447-454
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• 2012

본 실험에서는 propofol과 remifentanil 병용 마취법 사용 시 안압 및 혈액학적 변화를 측정하고 isoflurane 마취 결과와 비교하였다. 건강한 14마리의 비글견을 7마리씩 2군 (PRP군, ISF군)으로 나누었으며 PRP군은 마취 도입 10분 전에 acepromazine (0.05 mg/kg, IV)으로 전마취제 투여하고 atracurium 0.1 mg/kg 투여 후, propofol (5 mg/kg, IV)으로 마취 유도하였다. 마취 유지에는 propofol (0.2 mg/kg/min)과 remifentanil ($0.5{\mu}g/kg/min$)을 사용하였다. ISF군에서는 propofol (5~7 mg/kg, IV)로 마취를 유도하고, isoflurane 흡입마취법으로 마취를 유지하였다. 초기 isoflurane 농도를 3%으로 유지하다가 마취가 안정된 후 1.9%로 낮추어 유지 하였다. 모든 군에서 간헐적인 100% 양압 호흡을 사용해 $CO_2$는 38에서 45 mmHg사이를 유지하고 $SpO_2$는 95에서 100사이를 유지하였다. 총 마취 시간은 90분이었으며 안압, 혈압, 심박수를 각각 5, 10, 15, 30, 45, 60, 75, 90에 측정을 하였다. 실험 결과 propofol과 remifentanil 병용마취법이 isoflurane 흡입마취법 보다 안정적으로 안압과 혈압을 낮출 수 있었다.

• 한국임상수의학회지
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• 제29권3호
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• pp.237-241
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• 2012

대장통과시간은 소화기관 질환의 병태생리에 따른 유형분류와 치료계획을 설정하는데 매우 중요한 역할을 수행한다. 사람에게는 만성변비환자나 장운동에 문제가 있는 환자를 분류하기도 한다. 본 연구는 국내에서 개발된 비투과 표지자 Kolomark를 이용한 개에서 대장의 통과시간을 측정하여 정상범위에 대한 기준을 제시하고자 한다. 건강한 Beagle 11마리(암컷 10마리, 수컷 1마리)를 대상으로 콜로마크를 이용하여 2, 4, 8, 12, 24, 48시간마다 대장에 대한 잔류 개수와 통과 개수를 측정하였다. 대장에서 시간에 따른 콜로마크의 잔류량은 유의하였다. 콜로마크는 투여 후 4시간 후에 대장에 나타나기 시작하였고 8시간 후부터 잔류량의 유의한 차이가 확인되었다. 12시간 경과 후 대장내 콜로마크가 평균적으로 가장 많이 관찰되었다. 투여 후 24시간 후에는 약간의 잔류량이 확인 되었지만 48시간이 지난 후에는 대장 밖으로 모두 배출되었다. 콜로마크를 섭취후 배출까지 평균배출시간은 28.36시간이었다. 이번 연구를 통하여 콜로마크를 통한 개에서 대장통과시간의 정상 값을 확립하였으며 이러한 결과를 바탕으로 향후 변비환자의 치료 및 생리학적 검사를 보다 적극적으로 이용하는데 기초자료가 되리라 판단된다.

• 대한치과보철학회지
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• 제45권3호
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• pp.362-374
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• 2007

Statement of problem. The intial stability for osseointegration of implant has been an interesting factor. Especially, in the case of poor bone quality or immediately loaded implant, various strategies have been developed focusing on the surface of materials to improve implant fixation to bone. The microscopic properties of implant surfaces play a major role in the osseous healing of dental implants. Purpose. The aims of this study are to perform a histologic and histomorphometric comparison of the healing characteristics of three different surfaces and the comparison of resonance frequency analysis (RFA) values measured by $Osstell^{TM}$ and perio-test values (PTV) measured by Periotest. Material and methods. A total of 24 screw titanium implants (Dentium Co., Seoul, Korea) with 6mm in length and 3.4mm in diameter, were placed in the mandible of 4 beagle dogs. Implants were divided into three groups following the surface treatment methods: Group I is machined(control group). Group II is anodically oxidized. Group III is coated 500nm in thickness with hydroxyapatite(HA) by ion beam assisted deposition(IBAD) on the anodized oxidization. Bone blocks from 2 dogs were caught after 3 weeks of covered healing and another blocks from 2 dogs after 6 weeks. RFA values and PTV were measured right after insertion and at 3 and 6weeks. Histomorphometric analysis was made with Kappa Image Base System to calculate bone-to-implant contact (BIC) and bone area inside the threads. Pearson's correlation analyses were performed to evaluate the correlation between RFA and PTV, BIC and bone area ratio of three different surfaces at 3 and 6 weeks. Results. 1) In all surface treatment methods, the RFA values decreased and the PTV values increased until 6 weeks in comparison to initial values. 2) At 3 weeks, no significant difference was found from bone-to-implant contact ratio and bone area ratio of three different surface treatment methods(P>0.05). However, at 6 weeks, different surface treatment methods showed significantly different bone-toimplant contact ratio and bone area ratio(P<0.05). 3) In the implants with the IBAD on the anodic oxidization, significant difference was found between the 3 weeks and the 6 weeks bone area ratio(P<0.05). 4) Correlation was found between the RFA values and the bone area ratio at 3 and 6 weeks with significant difference(P<0.05). Conclusions. These results indicate that the implants with the IBAD on the anodic oxidization may have a high influence on the initial stability of implant.

• 한국임상수의학회지
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• 제22권1호
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• pp.21-25
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• 2005

This study was performed to assess therapeutic effect of the tibial plateau leveling osteotomy (TPLO) in dogs with experimentally transected cranial cruciate ligaments (CrCL). Nine healthy adult Beagle dogs were transected left CrCL under general anesthesia. The dogs were assigned to TPLO and non-TPLO control groups. The TPLO procedures for correcting the CrCL rupture in the left stifle of dogs were performed under sterile conditions. Before TPLO procedures, all dogs were screened by orthopedic and radiographic examinations. Dogs were lameness free for the previous three months, and when examined at the walk and trot on a hard surface, in a straight line and on a circle. Lateral and craniocaudal radiographs were done to confirm the soundness of the both knee joint in dogs and not detectable lesions were diagnosed. The dogs were intravenously injected with a 10 mci/kg of 99mTechnetium-methylene diphosphonate (99mTc-MDP) under general anesthesia. Scintigraphs were obtained using a large field of view gamma camera equipped a parallel-hole, low-energy about 3 hours after intravenous injection of 99mTc-MDP. Before CrCL transection and 4, 8, and 12 weeks after the procedures, scintigraphy were conducted. Bone uptake of the left stifle joint increased after the procedures in all dogs. When the bone uptake from the TPLO procedure was compared with that of the control, there was a significant difference (p < 0.05). At 12 weeks after the TPLO procedure, the dogs showed normal anatomical posture and gait. It is concluded that TPLO procedure was effective in reconstruct of the stifle joint in dogs with CrCL rupture.