• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1603-1611
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• 2009

High-cell-density cultivation of yeast was investigated using the agricultural waste products corn steep liquor (CSL) and molasses. The Saccharomyces cerevisiae KV-25 cell mass was significantly dependent on the ratio between C and N sources. The concentrations of molasses and CSL in the culture medium were statistically optimized at 10.25% (v/v) and 16.87% (v/v), respectively, by response surface methodology (RSM). Batch culture in a 5-l stirred tank reactor using the optimized medium resulted in a cell mass production of 36.5 g/l. In the fed-batch culture, the feed phase was preceded by a batch phase using the optimized medium, and a very high dried-cell-mass yield of 187.63 g/l was successfully attained by feeding a mixture of 20% (v/v) molasses and 80% (v/v) CSL at a rate of 22 ml/h. In this system, the production of cell mass depended mainly on the agitation speed, the composition of the feed medium, and the glucose level in the medium, but only slightly on the aeration rate.

• 한국미생물·생명공학회지
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• 제11권1호
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• pp.33-38
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• 1983

에타놀을 생산하는 Flocculent 균주는 균체순환을 위한 외부적인 장치 없이도 발효조 내부 자체에서 고농도의 균체를 유지시킬 수 있는 장점이 있다. 본 연구에서는 Flocculation 특성을 가지고 있는 Saccharomyces uvarum을 사용하여 Glucose 배지에서 회분식 및 연속식 발효특성을 고찰하였고 에타놀 발효의 수학적 모델을 만들었다. 이를 이용하여 여러 온도 및 pH에 따른 발효특성을 비교 검토한 결과 균체성장과 에탄올 생산의 최적온도는 33~35$^{\circ}C$ 이었고 pH 에 대한 영향은 그리 크지 않았으나 pH5가 최적 조건이었다. 이러한 환경적 최적 조건하에서 Semi-batch 발효에 의한 에타놀 최고 생산성은 12g/$\ell$/h이었다.

• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.972-981
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• 2009

In this paper, a fed-batch cultivation process in recombinant Escherichia coli BL21(DE3) bacteria, for the production of human soluble catechol-O-methyltransferase (hSCOMT), is presented. For the first time, a straightforward model is applied in a recombinant hSCOMT expression system and distinguishes an initial cell growth phase from a protein production phase upon induction. Specifically, the kinetic model predicts biomass, substrate, and product concentrations in the culture over time and was identified from a series of fed-batch experiments designed by testing several feed profiles. The main advantage of this model is that its parameters can be identified more reliably from distinct fed-batch strategies, such as glycerol pulses and exponential followed by constant substrate additions. Interestingly, with the limited amount of data available, the proposed model accomplishes satisfactorily the experimental results obtained for the three state variables, and no exhaustive process knowledge is required. The comparison of the measurement data obtained in a validation experiment with the model predictions showed the great extrapolation capability of the model presented, which could provide new complementary information for the COMT production system.

• 자원리싸이클링
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• 제10권2호
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• pp.27-33
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• 2001

무전해 Ni-Cu-P폐 도금액의 재사용에 대해 소정의 조건에서 조사하였다. 아연화처리한 후 니켈 촉매의 처리는 니켈 촉매처리를 하지 않았을 때 보다 도금시간이 연장되었다 Batch type에서 새로 제조한 도금액에 폐 도금액을 50% 첨가하여도 무전해 Ni-Cu-P 폐 도금액의 재사용이 가능하였다. 새로 제조한 도금액에 소모된 도금액의 성분을 연속적으로 보충하여 도금하면(Continuous type), 보충하지 않았을 경우(B기ch type)보다 도금시간이 10배 연장되었다. 새로 제조한 도금액에 폐 도금액 50%를 첨가하여 소모된 도금액의 성분을 연속적으로 보충할 경우(Continuous type)의 도금시간은 보충하지 않았을 경우(Batch type)의 도금시간 보다 3.7배 연장되었다. 도금층의 불량과 급격한 도금속도의 감소는 도금층의 Ni과 Cu의 성분 변화에 큰 영향을 미쳤다.

• 한국환경과학회지
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• 제20권1호
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• pp.35-47
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• 2011

The effect of the variation of aeration time on the removal of organics, nitrogen and phosphorus using synthetic wastewater was investigated in sequencing batch reactors (SBRs) which included DNPAOs and DNGAOs. The cycling times in four SBRs were adjusted to 12 hours and then included different aerobic times as 1 hr, 2.5 hr, 4 hr and 5.5 hr, respectively. Four SBR systems have been operated and investigated for over 40 days. Average TOC removal efficiencies were about 71 % in all SBRs. The $NH_4^+$-N removal efficiency was increased as the increase of aeration time. After changing aeration time, the total nitrogen removal efficiencies of SBRs were shown as 35 %, 85 %, 75 % and 65 %, respectively. Higher phosphorus release and uptake were occurred as the decrease of the aeration time. After all, the overall phosphorus removal efficiency decreased and the deterioration of phosphorus removal was occurred when aeration time was over 4 hr. Denitrification in aerobic conditions was observed, which showed the presence of DNPAOs and DNGAOs. In batch experiments, PAOs were shown as the most important microorganisms for the phosphorus removal in this experiment, and the role of DNGAOs was higher than that of DNAPOs for the nitrogen removal.

• 한국지반환경공학회 논문집
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• 제4권3호
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• pp.61-67
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• 2003

축산폐수를 처리 시 우선 탈수 후 고상은 퇴비화, 액상을 연속회분식반응기(Sequencing batch reactor, SBR)로 이용하여 처리하는 시스템을 구상하였다. 영양염류 제거를 위한 SBR 공정의 안정적인 운전을 위한 운전모드 결정 실험을 수행하였다. K시 공공축산폐수처리장의 원심분리기에서 나온 유출수를 사용한 실험에서 질소를 제거하기 위한 적정 fill ratio는 1/12, SRT는 15일, 폭기/비폭기 주기는 2시간/1시간이었다. 탈질을 위하여 주입한 외부탄소원으로는 메탄올을 사용하였고 single feeding 방법과 step feeding 방법을 사용하였다. 이 결과 step feeding 방법을 사용시 더 효과적으로 유기물을 사용 탈질효율을 증가시킬 수 있었다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1999년도 제14차 학술회의논문집
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• pp.57-60
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• 1999

An optimal control technique designed for simultaneous tracking and quality control for batch processes. The proposed technique is designed by transforming quadratic-criterion based iterative learning control(Q-ILC) into linear quadratic control problem. For real-time quality inferential control, the quality is modeled by linear combination of control input around target qualify and then the relationship between quality and control input can be transformed into time-varying linear state space model. With this state space model, the real-time quality inferential control can be incorporated to LQ control Problem. As a consequence, both the quality variable as well as other controlled variables can progressively reduce their control error as the batch number increases while rejecting real-time disturbances, and finally reach the best achievable states dictated by a quadratic criterion even in case that there is significant model error Also the computational burden is much reduced since the most computation is calculated in off-line. The Proposed control technique is applied to a semi-batch reactor model where series-parallelreactions take place.

• 한국수소및신에너지학회논문집
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• 제24권2호
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• pp.99-106
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• 2013

The variations of performance and metabolites at an early stage were investigated for the successful start-up technology in continuous fermentative hydrogen production. Unsuccessful start-up was observed when the operation mode was changed from batch to continuous mode after the yield was reached to 0.5 mol $H_2$/mol $hexose_{added}$ by batch mode. $H_2$ production continued till 12 hours accompanied by butyrate production, but did not last with propionate production increase. It was suspected that the failure was due to the regrowth of propionic acid bacteria during batch mode which were inhibited by heat-shock but not completely killed. Thus, successful start-up was tried by early switchover from batch to continuous operation; continuous operation was started after the $H_2$ yield was reached to 0.2 mol $H_2$/mol $hexose_{added}$ by batch mode. Although $H_2$ production rate decreased at an early stage, stable $H_2$ yield of 0.8 mol $H_2$/mol $hexose_{added}$ was achieved after 10 days by lowering down propionate production. And it was also concluded that the reason for $H_2$ production decrease at an early stage was due to alcohol production by self detoxification mechanism against VFAs accumulation.

• KSBB Journal
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• 제18권5호
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• pp.418-423
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• 2003

발효조의 냉각수 공급 제어 신호와 냉각수의 유출입 지점의 온도를 온라인으로 측정하여 이 값들로부터 냉각량을 추정할 수 있는 실험식을 얻었다. 회분식 배양을 통하여 냉각량이 온라인으로 측정될 수 있음을 확인하였고 기질의 재투입시 냉각량의 변화를 통하여 유가배양의 제어변수로 활용할 수 있음을 점검하였다. 미리 프로그램된 목표 냉각량의 증가곡선에 따라 냉각량이 증가되면서 세포가 자라도록 유가배양을 실시하였다. 본 연구를 통하여 냉각량의 온라인 측정방법을 제시하였고 이를 유가배양 제어변수로 활용하여 세포의 성장속도를 조절할 수 있음을 보였다.

• 한국염색가공학회지
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• 제28권3호
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• pp.175-182
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• 2016

To investigate dyeing behaviors in accordance with dye structure in cold pad batch dyeing of cotton knit, monochlorotriazine-vinylsulfone bifunctional dyes and monofluorotriazine-vinylsulfone bifunctional dyes were prepared. The spectral property and solubility of the dyes were tested and compared with the imported dyes. In addition, exhaustion behaviors of individual and mixed dye solutions were measured to examine the influence of dye structure on dyeing behavior in cold pad batch dyeing. The substantivity, fixation, migration index and half dyeing time were also calculated for further analysis of dyeing behavior of the prepared dyes. As a result, both dyes exhibited the superior solubilities and satisfactory light absorption properties. Also, monofluorotriazine-vinylsulfone bifunctional dyes showed moderate sensitivity to alkalinity and proper kinetic index values compared with the monochlorotriazine-vinylsulfone bifunctional dyes and the imported dyes. The results indicated monofluorotriazine-vinylsulfone bifunctional structure of the dye is suitable for cold pad batch dyeing.