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Electrochemical Studies of Immobilized Laccases on the Modified-Gold Electrodes

  • Yoon Chang-Jung;Kim Hyug-Han
    • Journal of the Korean Electrochemical Society
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    • v.7 no.1
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    • pp.26-31
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    • 2004
  • The direct electrochemical studies of four laccases (plant and fungal laccases) have been investigated on a gold electrode functionalized with a new tether of 2.2'-dithiosalicylic aldehyde. Results from these studies indicate that the redox potential of the active site of plant laccase from Rhus vernificera is shifted to a more negative value(255 mV versus SCE) than that of fungal laccase from Pyricularia oryzae (480 mV versus SCE). Mechanistic studies indicate that the reduction of type-1 Cu precedes the reduction of type-2 and type-3 Cu ions when the electrode is poised initially at different potentials. Also a new tether, 2.2'-dithiosalicylic aldehyde, has been used to study the redox properties of two laccases (LCCI and Lccla) covalently attached to a gold electrode. An irreversible peak at 0.47V vs. SCE is observed in the cyclic voltammorams of LCCI. In contrast, the cyclic voltammograms of LCCIa contain a quasi-reversible peak at 0.18V vs. SCE and an irreversible peak at 0.50V vs. SCE. We find that the replacement of the eleven amino acids a the C-terminus with a single cysteine residue $(i.e., \;LCCI{\rightarrow}LCCIa)$ influences the rate of heterogeneous electron transfer between an electrode and the copper containing active sites $(K_{het}\;for\;LCCI=1.0\times10^{-2}\;s^{-1}\;and\;K_{het}\;for\;LCCI_a= 1.0\;times10^{-1}\;s^{-1}\'at\;0.18V\;versus\;SCE\;and\;4.0\times10^{-2}\;s^{-1}\;at\;0.50V\; versus\;SCE)$. These results show for the first time that the change of the primary structure of a protein via site-directed mutagenesis influences both the redox potentials of the copper ions in the active site and the rate of heterogeneous electron transfer.

The Comparison on the Compression Measurement Value of Medical Compression Stockings (수입 의료용 압박스타킹의 압력 측정치 비교)

  • Do, Wol-Hee;Kim, Nam-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.37 no.8
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    • pp.1060-1074
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    • 2013
  • This study measured and analyzed pressure at each measurement part of imported compression stockings sold in Korea to provide basic information to establish a pressure standard and grade ranking. This study used 40 medical compression stockings imported from 6 countries. Pressure measurements were taken at 11 points: front side and back side of ankle, end-point of the gastrocnemius muscle, front, inner side, back, and outer side of calf, back side of below knew girth, inner side, and outer side of mid-thigh girth, and inner side of thigh girth. AMI 3037-10 and AMI 3037-2 were used for measurements taken inside an environmental chamber at a temperature of $21^{\circ}C$ and a relative humidity (RH) of 65%. For the measurements, 11 air pack sensors were attached to a wooden model leg (Hohenstein) and three measurements were taken at each measurement point in three minutes. The average of these measurements was used for analysis. The findings of this study were as follows. As for the front side of the ankle, of the 40 products, 14 products (6 USA, 2 Swiss, 3 Italian, and 2 Taiwanese) were within the pressure range indicated on the product label; however, no German products fell within the pressure range. A total of 8 products (5 USA, 1 Swiss, 1 Italian, and 1 German) were gradient compression type; however, no Japanese or Taiwanese product were of this type. The majority of products had the highest pressure at the end-point of the gastrocnemius muscle. Only 3 products, 1 USA (Jobst Opaque 30-40mmHg), 1 Swiss (Sigvaris Cotton 34-46mmHg) and 1 Italian (Jobstocking 25-32mmHg), had measurements that met the indicated standard pressure, were a gradient compression type, and met the overall standard for compression stockings.

Ring-type Heart Rate Sensor and Monitoring system for Sensor Network Application (센서 네트워크 응용을 위한 반지형 맥박센서와 모니터링 시스템)

  • Jang, In-Hun;Sim, Kwee-Bo
    • Journal of the Korean Institute of Intelligent Systems
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    • v.17 no.5
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    • pp.619-625
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    • 2007
  • As low power, low cost wireless communication technology like Bluetooth, Zigbee, RFID has been put to practical use together with the wellbeing trend, the concern about ubiquitous health care has been greatly increased and u-Health is becoming one of the most important application in the sensor network field. Especially, development of the medical services to be able to cope with a state of emergency for solitary senior citizens and the aged in silver town is very meaningful itself and their needs are also expected to continuously increase with a rapid increase in an aging population. In this paper we demonstrate the feasibility of extracting accurate heart rate variability (HRV) measurements from photoelectric plethysmography(PPG) signals gathered by a ring type pulse oximeter sensor attached to the finger. For this, we made 2 types of ring sensor, that is reflective and pervious type, and developed the remote monitoring system which is able to collect HR data from ring sensor, analyze and cope with a state of emergency.

Lineage of Horse Bridle Kept in Yatsushiro Shrine in Kamishima (가미시마 야츠시로 신사(神島八代神社) 소장 재갈의 계보)

  • Shimizu, Yasuji
    • Korean Journal of Heritage: History & Science
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    • v.49 no.4
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    • pp.156-179
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    • 2016
  • Yatsushiro Shrine on the island of Kamishima located in Ise Bay, Japan, contains many cultural artifacts with ancient mirrors in significant numbers. Also included among the artifacts are horse gags and reins that are clearly of the same lineage as the horse harnesses from the unified Shilla era and Goryeo era in Korean history. Type classification and position establishment were carried out on the horse reins kept in the Yatsushiro shrine, including items such as those mentioned previously. Type A horse harness can be classified into 3 types. Based on this classification scheme, the harness type in the Yatsushiro shrine was found to belong to the most recent period. The blacksmith workshop that made the harness was producing iron wares and bronze wares, and it possessed metal forging and metal casting technologies. Note, however, that it was primarily a workshop where iron wares were made. The parts that were visible from the outside when the harness was attached were made from composite bronze, and the reins and bridle linkage were made from iron. Such integration of bronze ware production techniques and iron ware production techniques was an attempt at enhancing the practicality and embellishments on the harness.

Assessment of stem cell viability in the initial healing period in rabbits with a cranial bone defect according to the type and form of scaffold

  • Kang, Seung-Hwan;Park, Jun-Beom;Kim, InSoo;Lee, Won;Kim, Heesung
    • Journal of Periodontal and Implant Science
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    • v.49 no.4
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    • pp.258-267
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    • 2019
  • Purpose: Increased bone regeneration has been achieved through the use of stem cells in combination with graft material. However, the survival of transplanted stem cells remains a major concern. The purpose of this study was to evaluate the viability of transplanted mesenchymal stem cells (MSCs) at an early time point (24 hours) based on the type and form of the scaffold used, including type I collagen membrane and synthetic bone. Methods: The stem cells were obtained from the periosteum of the otherwise healthy dental patients. Four symmetrical circular defects measuring 6 mm in diameter were made in New Zealand white rabbits using a trephine drill. The defects were grafted with 1) synthetic bone (${\beta}$-tricalcium phosphate/hydroxyapatite [${\beta}-TCP/HA$]) and $1{\times}10^5MSCs$, 2) collagen membrane and $1{\times}10^5MSCs$, 3) ${\beta}-TCP/HA+collagen$ membrane and $1{\times}10^5MSCs$, or 4) ${\beta}-TCP/HA$, a chipped collagen membrane and $1{\times}10^5MSCs$. Cellular viability and the cell migration rate were analyzed. Results: Cells were easily separated from the collagen membrane, but not from synthetic bone. The number of stem cells attached to synthetic bone in groups 1, 3, and 4 seemed to be similar. Cellular viability in group 2 was significantly higher than in the other groups (P<0.05). The cell migration rate was highest in group 2, but this difference was not statistically significant (P>0.05). Conclusions: This study showed that stem cells can be applied when a membrane is used as a scaffold under no or minimal pressure. When space maintenance is needed, stem cells can be loaded onto synthetic bone with a chipped membrane to enhance the survival rate.

Physical and Biological Performance Evaluation of Disinfection Systems for Transportation Vehicles against AI Virus

  • Chung, Hansung;Choi, Kwanghoon;Kim, Sungkwan;Kim, Sukwon;Lee, Kyungwoo;Choe, Nonghoon
    • Journal of Microbiology and Biotechnology
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    • v.31 no.7
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    • pp.956-966
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    • 2021
  • To prevent the outbreak of infectious diseases that inflict huge economic and social losses, domestic livestock farms and related facilities have introduced automatic and semiautomatic disinfectant solution-spraying systems for vehicles. However, the facility standards and specifications vary by manufacturer, and no scientific performance evaluation has been conducted. The puropose of this study is to develop physical and biological evaluation methods. Physical and biological appraisals were conducted using two types of disinfection facilities (tunnel- and U-type) and two types of vehicles (passenger car, truck). Water-sensitive paper was used to evaluate the physical performance values for the disinfection facilities. In addition, to assess their biological performance, carriers containing low-pathogenic avian influenza virus were attached to vehicles, and the viral reduction was measured after the vehicles moved through the facility. The tunnel-type had rates of coverage in the range of 70-90% for the passenger car and 60-90% for the truck. At least 4-log virus reduction after spraying for 1-5 min was shown for both vehicles. For the U-type facility evaluation, the coverage rates were in the range of 60-90% for the passenger car and at least 90% for the truck. More than 4-log viral reduction was estimated within a spraying time of 5 min. To reduce viruses on the surface of vehicles by at least 4 log within a short period, the disinfectant solution should cover at least 71% of the pathogens. In conclusion, we were able to assess the physical and biological performance criteria for disinfection facilities aboard transportation vehicles.

Towed Underwater LDV Measurement of the Interaction of a Wire-Type Stimulator and the Boundary Layer on a Flat Plate (예인수조 LDV를 이용한 평판 경계층과 와이어 타입 난류촉진장치의 상호작용에 관한 연구)

  • Park, Jongyeol;Seo, Jeonghwa;Rhee, Shin Hyung
    • Journal of the Society of Naval Architects of Korea
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    • v.58 no.4
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    • pp.243-252
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    • 2021
  • The present study aims to investigate the interaction of a wire-type turbulence stimulator and the laminar boundary layer on a flat plate by flow field measurement. For the towing tank tests, a one-dimensional Laser Doppler Velocimetry (LDV) attached on a two-axis traverse was used to measure the streamwise velocity component of the boundary layer flow in zero pressure gradient, disturbed by a turbulence stimulator. The wire diameter was 0.5 and 1.0 mm according to the recommended procedures and guidelines suggested by the International Towing Tank Conference. Turbulence development by the stimulator was identified by the skin friction coefficient, mean and Root Mean Square (RMS) of the streamwise velocity. The laminar boundary layer with the absence of the wire-type stimulator was similar to the Blasius solution and previous experimental results. By the stimulator, the mean and RMS of the streamwise velocity were increased near the wall, showing typical features of the fully developed turbulent boundary layer. The critical Reynolds number was reduced from 2.7×105 to 1.0×105 by the disturbances caused by the wire. As the wire diameter and the roughness Reynolds number (Rek) increased, the disturbances by the stimulator increased RMS of the streamwise velocity than turbulent boundary layer.

Behavior of a Shape Memory Alloy Actuator with Composite Strip and Spring (복합재료 스트립과 스프링을 갖는 형상기억합금 작동기의 거동)

  • Heo, Seok;Hwang, Do-Yeon;Choi, Jae-Won;Park, Hoon-Cheol;Goo, Nam-Seo
    • Composites Research
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    • v.22 no.2
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    • pp.37-42
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    • 2009
  • This paper presents an experimental approach to design a bending-type actuator by using a shape memory alloy wire (SMA), composite strip, and spring. The SMA wire is attached to two edges of the bent strip to apply pre-stress to the SMA wire. The spring is used to provide recovery force right after actuation of the SMA wire. To investigate thermo-mechanical characteristics of the SMA wire, a series of DSC tests have been conducted and tensile tests under various levels of pre-stress and input power have been performed. Based on the measured properties of the SMA wire, bending-type actuators are designed and tested for different combination of strip, number of springs, and input power. It has been found that a bending-type actuator with a proper combination shows fast actuation performance and low power consumption.

A Comparative Study on the Retention of Implant Overdenture According to the Shape and the Number of Magnetic Attachment (자성 어태치먼트의 형태와 수에 따른 하악 임플란트 피개의치의 유지력에 대한 비교 연구)

  • Seo, Min-Ji;Lee, Joon-Seok;Cho, In-Ho
    • Journal of Dental Rehabilitation and Applied Science
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    • v.24 no.2
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    • pp.169-181
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    • 2008
  • The aim of this study was to compare the retention and stability of implant overdenture according to the shape and the number of magnetic attachment. The experimental groups were designed for the number of implants(1, 2, 4) and shape of magnetic attachments(flat, cushion, dome type) resulting in 9 subgroups. 45 attachments were tested attached to $Br{\aa}nemark$ system implants which were planted on a mandibular model. Each attachment was composed of the magnet assembly embedded in a overdenture sample and the abutment keeper screwed into the implants. Dislodging tensile forces were applied to the overdenture samples using an Instron(cross-head speed 50.80mm/min) in 3 directions simulating function: vertical, oblique, and anterior-posterior. The loading was repeated 10 times in each direction for 45 samples. The values of maximum dislodging force of each subgroup were processed statistically using SPSS V. 12.0 at the 0.05 level of significance. The results of this study were as follows: 1. Flat type magnetic overdenture was the most retentive when subjected to vertically directed forces and dome type was the lest retentive when subjected to obliquely directed forces(p<0.05). 2. In case of planting one implant, flat type had a higher vertically retentive force than anterior-posteriorly retentive force. In case of planting two implants, flat type and dome type had a higher vertically retentive force and in case of planting four implants, flat type and cushion type had a higher vertically retentive force than anterior-posteriorly retentive force(p<0.05). 3. The incremental number of dental implant, without regards to the three types of magnetic attachment shapes, showed higher retention of overdenture(p<0.05). From the results, if a patient need much more retention of implant overdenture, flat type magnetic overdenture would be a good treatment. In case of the bruxism where excessive lateral forces are already present, dome type could be expected to produce better results. In case of planting one implant, flat type is more stable than the other shape of magnet and in case of two implant, flat type and dome type are more stable and in case of four implants, flat type and cushion type are more stable. Planting more than two implants and using flat type magnetic attachment would provide better retention and stability of implant overdenture

The Facade Improvement of Complexed Commercial Building Considering Open Signboard - Focused on Commercial district in Chnagwon - (옥외광고물 설치를 고려한 복합상업건물 입면개선 - 창원시 일반상업지구를 중심으로 -)

  • Yu, Jin-Sang;Seo, You-Seok
    • Archives of design research
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    • v.20 no.3 s.71
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    • pp.191-202
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    • 2007
  • In these days, open signboard system is controlled by administrative policy and law, but there is no consideration for different types of building. As such, this study aims to propose planning criteria for streetside commercial buildings, such as elevation and mass design of buildings, layout of signboard attached to the building elevation with consideration for streetscape. In mass planning for streetside commercial buildings, the building type with front open space keeps lower open signboard density than the building type directly leading to the street. It is desirable that open signboard of lower floor part is attached by a horizontal type, open signboard of low medium floor part by a projected vertical type, open signboard of high medium floor part and roof part with a minumum attachment of open signboard. As for elevation planning relative to open signboard, it is desirable that an irregular wall type is more useful than a regular wall type to control open signboard. And in all cases, horizontal element facade has a handicap to control the quantity of signboard. If the building has a corner, the piloti should be used in the corner of lower story for smooth circulation of pedestrians and emphasizing the transparency of elevation. Specially, in the case of a round corner, the corner should be emphasized by the composition of high transparent mass.

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