• Title/Summary/Keyword: antibacterial property

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Analysis of Physical and Antibacterial Properties of Functional Silicone Hydrogel Ophthalmic Lenses Containing Graphene Groups

  • Su-Mi Shin;Hye-In Park;A-Young Sung
    • Korean Journal of Materials Research
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    • v.33 no.1
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    • pp.1-7
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    • 2023
  • The physical and antibacterial properties of ophthalmic lenses fabricated by copolymerization with hydrogel monomers using two types of graphene were measured, and their usability as contact lens materials was analyzed. For polymerization, silicone monomers, including SID-OH, 3-(methacryloxy)propyl tris(trimethylsiloxy)silane, and decamethylcyclopentasiloxane, were used, and N,N-dimethylacetamide, ethylene glycol dimethacrylate as a crosslinking agent, and azobisisobutyronitrile as an initiator were added. Also, graphene oxide nanoparticle (GON) and graphene nanoplate (GNP) were used as an additive, and the physical properties of the lenses fabricated after copolymerization were evaluated. The fabricated lenses satisfied the basic physical properties of general hydrogel contact lenses and showed the characteristics of lenses with high water content, and the disadvantage of very weak durability, due to low tensile strength. However, it was confirmed that the tensile strength and antibacterial properties were greatly improved by adding GON and GNP. With GON, the oxygen permeability and refractive index of the fabricated lenses were slightly improved. Therefore, it was determined that the graphene materials used in this study can be used in various ways as a contact lens material.

Development and Utilization of Eco-friendly Products based on Hemp Fabrics (대마 기반 친환경 의류 제품의 개발 및 활용에 관한 연구)

  • Su-Hyun Kim;Hee-Sook Kim
    • Fashion & Textile Research Journal
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    • v.25 no.1
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    • pp.62-71
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    • 2023
  • Recently, interest in natural fabric materials that are not harmful has increased, and hemp is being studied as a new eco-friendly product. This study produced hemp fabric with improved flexibility and increased antibacterial properties by blending it with Hanji yarn. Various weaving methods were proposed to overcome the rough physical properties of hemp, and the functions of the developed products were evaluated through antibacterial tests. The mixing ratios of hemp and Hanji yarns was 50% hemp: 50% Hanji weft, 70% hemp: 30% Hanji weft, 30% hemp: 70% Hanji weft, and 100% hemp. Overall, the higher the ratio of Hanji yarn, the higher the fastness property, and the higher the ratio of hemp yarn, the higher the flexibility of the fabric, which was evaluated to be comfortable to wear. The 99.9% antibacterial properties of hemp products were considered to contribute to maintaining the health of modern people. Owing to its high intensity and high air permeability, it is considered highly usable in the production of children's clothing with a lot of activity. It was evaluated as an advantage that the disadvantage of hemp, which was limited as a material for summer clothing, was broadened to use for all seasons due to the fusion of Hanji. Otherwise, low consumer satisfaction as an outdoor wear is a disadvantage because hemp products had low elasticity and wrinkles.

Antibacterial Property of Ecklonia cava Extract against Marine Bacterial Pathogens (해양 유해세균에 대한 감태 추출물의 항균특성)

  • Kim, Ji-Hoon;Kim, Se-Bong;Hwang, Hye-Jin;Kim, Young-Mog;Lee, Myung-Suk
    • Journal of Food Hygiene and Safety
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    • v.31 no.5
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    • pp.380-385
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    • 2016
  • The object of this study was to evaluate antibacterial activity of edible seaweed extracts against marine bacterial pathogens including Edwardsiella tarda, Streptococcus iniae, Streptococcus parauberis, Vibrio anguillarum, Vibiro harveyi and Vibrio scophthalm, which are associated with human or fish infectious disease. Ecklonia cava methanolic extract showed a strong and broad spectrum antibacterial activity against marine bacterial pathogens used in this study. Among solvent-soluble fractions of the E. cava extract, the ethyl acetate (EtOAc) soluble fraction showed the strongest antibacterial activity against marine bacterial pathogens tested in this study with MIC in the range of $128-256{\mu}g/mL$. Furthermore, HPLC analysis revealed that the soluble fraction contains abundant dieckol, a phlorotannin compound, compared to other solvent soluble fractions, suggesting that phlorotannins including dieckol would be a key antibacterial agent against marine bacterial pathogens.

Antibacterial Function of Fabrics Dyed with Extract from Chamaecyparis obtusa Leaves against Methicillin-resistant Staphylococcus aureus (편백나무 잎 추출액을 이용한 천연염색포의 항생제 내성균주에 대한 항균성)

  • Choi, Na Young;Kim, Ji-Hee
    • Fashion & Textile Research Journal
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    • v.19 no.3
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    • pp.331-336
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    • 2017
  • Bacteria exist everywhere and continuously come into contact with daily surroundings and humans. Super bacterium methicillin-resistant Staphylococcus aureus, resistant to methicillin, has recently appeared. The morbidity and rate of death associated with super bacteria infection has increased. This study investigated the antibacterial activity of fabrics naturally dyed with Chamaecyparis obtusa leaves extract against methicillin-resistant Staphylococcus aureus. Fabrics were left for 15 min in a natural dyeing solution prepared by extraction from C. obtusa leaves using 11.3% (o.w.f) with a fixed liquor ratio of 1:22 at $40^{\circ}C$. The dyeing process was conducted using three different mordants; subsequently, the K/S value of the dyed fabrics increased in the order of None < Cu < Fe < Al. The color fastness property of the fabrics to washing, dry-cleaning, and rubbing was found to be excellent and ranked in the 4-5 grade. The color fastness to light of natural dyeing is low in most cases and has the problem that the dye color soon becomes bleached. Yet, in most cases cloth dyed with retinispora leaves, the color fastnezz to light was good with a third to fourth grade. Non-mordant fabrics, aluminum mordants, and copper mordants also showed better antibacterial properties (99.9% reduction) against methicillin-resistant Staphylococcus aureus, compared to the control fabrics. The dyed fabrics showed the same antibacterial activity even after three washes. The results highlight the strong potential of fabrics naturally dyed with C. obtusa-extract as a medicinal material with excellent antibacterial function against methicillin-resistant Staphylococcus aureus.

Study on the Change in Physical and Functional Properties of Paper by the Addition of Chitosan (키토산 섬유를 첨가한 종이의 물성 및 기능성의 변화에 관한 연구)

  • Park, Seong-Cheol;Kang, Jin-Ha;Lim, Hyun-A
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.42 no.5
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    • pp.37-46
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    • 2010
  • This study was carried out to develop new application field and obtain the basic data of mixed paper with wood pulp and chitosan fiber for producing functional paper. Two types of wood pulp, such as SwBKP and HwBKP, were mixed with chitosan fiber. Physical and optical properties, water vapor absorption, air permeability, antibacterial activity and ash were measured. And the surface morphology of manufactured paper was observed using SEM. The results are as follows. It was revealed that density, breaking length, burst index, tear index, folding endurance and brightness were reduced but water vapor absorption and air permeability were on the rise in the structural view of SwBKP according to increasing the chitosan fiber ratio. Those HwBKP added chitosan fiber were great not only in the strength but also water vapor absorption and air permeability except for brightness. The water vapor absorption was lower and the air permeability was higher in the HwBKP added various chitosan fiber ratios than those with no chitosan fiber. It is estimated that these properties were related with various mixed rate of chitosan fiber. Particularly, air permeability was strongly dependent on the mixed rate of chitosan fiber. The chitosan fiber has superior antibacterial property, comparing with wood fiber. Adding chitosan fiber to the wood pulp was found to have an excellent antibacterial activity, more than 90%. The ashes were determined within 0.5%. Special bonds between chitosan fiber and wood pulp was observed by SEM and it means that the chitosan fiber were combined equally in the interior of wood pulp. In conclusion, mixing wood pulp with chitosan fiber can not only improves the quality of paper but also extend the usage of paper as a functional paper by using inherent property of chitosan. After all, production of functional paper added chitosan fiber is expected for new valuable industry of paper.

Antibacterial properties of traditional ceramic glazes containing copper oxide (산화구리를 함유하는 전통 세라믹 유약의 항균특성에 관한 연구)

  • Kim, Ung-Soo;Choi, Jung-Hoon;No, Hyung-Goo;Han, Kyu-Sung;Kim, Jin-Ho;Hwang, Kwang-Taek
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.29 no.6
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    • pp.372-378
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    • 2019
  • Traditional ceramic glazes formulated with copper oxide (CuO) exhibited antibacterial properties on Staphylococcus aureus (Gram Positive) and Escherichia coli (Gram Negative). All the ceramic glazes containing CuO showed antibacterial behavior when fired in reducing atmosphere. However, some of copper glazes presented antibacterial behavior and had no antibacterial properties at all when sintered in an oxidizing atmosphere. To elucidate the antibacterial mechanism, ceramic glazes were studied for phase and microstructure analysis, dissolution behavior and surface zeta potential. Metallic copper was precipitated in the glaze layer when sintered in reducing atmosphere. Less than 0.05 ppm of Cu ion was dissolved from glazes. Ca ion was most dissolved among all the samples. Glaze surface was highly negatively charged when CuO was added over 3 wt.% regardless of the sintering atmosphere. The antibacterial behavior of ceramic glazes seemed to be directly related to the dissolution behavior of cations, but the antibacterial behavior of oxidized specimens was not explained by the dissolution behavior. Surface potential of ceramic glazes appeared to play an auxiliary role in antibacterial properties.

Adsorption and Antibacterial Properties of Metal Treated Activated Carbon (금속 처리된 활성탄소의 흡착과 항균특성)

  • Oh, Won-Chun;Kim, Bum-Soo;Lee, Young-Hoon;Kim, Jong-Gyu;Kim, Myung-Kun;Ko, Young-Shin
    • Analytical Science and Technology
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    • v.11 no.4
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    • pp.266-270
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    • 1998
  • The Ag-treated activated carbon was prepared by the impregnation of Ag on the home made activated carbon. We investigated the nitrogen adsorption property, surface structure, and antibacterial activity of the carbon. The BET surface areas of Ag impregnated activated carbon are distributed to $740-1112.2m^2/g$ region. The results of nitrogen adsorption property show that BET surface areas move gradually to lower value with increasing $AgNO_3$ mole concentration. From the SEM results, we observed window blocking effect for micropores of external surface of adsorbent by Ag impregnation. Escherichia coli which is a kind of colon bacillus was used as bacteria for antibacterial test. From these results, we also observed that activity increase gradually to larger range with increasing $AgNO_3$ mole ratio.

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Structural and Functional Characterization of CRAMP-18 Derived from a Cathelicidin-Related Antimicrobial Peptide CRAMP

  • Park, Kyong-Soo;Shin, Song-Yub;Hahm, Kyung-Soo;Kim, Yang-Mee
    • Bulletin of the Korean Chemical Society
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    • v.24 no.10
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    • pp.1478-1484
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    • 2003
  • CRAMP was identified from a cDNA clone derived from a mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptide. Tertiary structure of CRAMP in TFE/$H_2O$ (1 : 1, v/v) solution has been determined by NMR spectroscopy previously and consists of two amphipathic $\alpha-helices$ from Leu4 to Lys10 and from Gly16 to Leu33. These two helices are connected by a flexible region from Gly11 to Gly16. Analysis of series of fragments composed of various portion of CRAMP revealed that an 18-residue fragment with the sequence from Gly16 to Leu33 (CRAMP-18) was found to retain antibacterial activity without cytotoxicity. The effects of two Phe residues at positions 14 and 15 of CRAMP-18 on structure, antibacterial activity, and interaction with lipid membranes were investigated by $Phe^{14,15}$ ${\rightarrow}$ Ala substitution (CRAMP-18-A) in the present study. Substitution of Phe with Ala in CRAMP-18 caused a significant reduction on antibacterial and membrane-disrupting activities. Tertiary structures of CRAMP-18 in 50% TFE/$H_2O$ (1 : 1, v : v) solution shows amphipathic ${\alpha}$-helix, from $Glu^2{\;}to{\;}Leu^{18}$, while CRAMP-18-A has relatively short amphipathic ${\alpha}$-helix from $Leu^4{\;}to{\;}Ala^{15}$. These results suggest that the hydrophobic property of $Phe^{14}{\;}and{\;}Phe^15$ in CRAMP-18 is essential for its antibacterial activity, ${\alpha}$-helical structure, and interactions with phospholipid membranes.

Variation of Antimicrobial Peptide in the Extract of the Hard-shelled Mussel Mytilus coruscus Depending on Boiling (가열 유무에 따른 참담치(Mytilus coruscus) 추출물 내의 항균 펩타이드 변화)

  • Lee, Ji-Eun;Seo, Jung-Kil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.6
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    • pp.875-885
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    • 2022
  • This study was performed to confirm the optimal extraction method for antimicrobial peptides from the Hard-shelled mussel. Extractions were performed with two processes including 1% HAc/boiling and 1% HAc/non-boiling methods and used extracts for the comparison of the antimicrobial activity, protease stability, action mechanism, AU-PAGE (acid-urea PAGE), and HPLC chromatograms. 1% HAc/boiling extract showed potent antibacterial activities both against Gram-positive and negative bacterium but 1% HAc/non-boiling extract showed antibacterial activity only against Gram-positive bacteria. Treatment of 1% HAc/boiling extract with proteases retained almost antibacterial activity against B. subtilis, but abolished significant antibacterial activity against E. coli D31. Only 1% HAc/boiling extract showed two discrete clearing antibacterial zones including slow migrating and rapid migrating zones. Both extracts showed strong DNA-binding ability but did not show bacterial membrane permeabilizing ability. In comparison of the chromatogram obtained from C18 or cation-exchange HPLC, the eluted peaks from 1% HAc/boiling extract showed high hydrophobic property or absorbance compared to 1% HAc/non-boiling extract, respectively. The concentration of the purified antimicrobial peptide was also higher in 1% HAc/boiling extract than in 1% HAc/non-boiling extract. Our results suggest that the effective extraction condition for antimicrobial peptides from marine invertebrate is boiling process in a weak acetic acid solution (1%).

Properties of LDPE Composite Films Using Polyurushiol (YPUOH) for Functional Packaging Applications (폴리우루시올(YPUOH)을 이용한 기능성 패키징용 LDPE 복합필름의 특성분석에 관한 연구)

  • Jung, Suyeon;Kim, Dowan;Seo, Jongchul
    • Applied Chemistry for Engineering
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    • v.26 no.1
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    • pp.23-28
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    • 2015
  • Lacquer sap extracted from lacquer trees exhibits good thermal stabilities and antimicrobial properties. To apply these superior properties to functional packaging, polyurushiol (YPUOH) powders were prepared and blended into LDPE (low density polyethylene) to prepare three different LDPE/YPUOH composite films via a twin screw extruder system. Their morphology, thermal and antimicrobial properties as well as barrier properties of the LDPE/YPUOH composite films were thoroughly investigated to find out applicablities of the films as functional packaging materials. Although the interfacial interaction between LDPE and YPUOH was relatively weak, LDPE/YPUOH composite films exhibited good dispersion of YPUOH in LDPE, resulting in the enhanced thermal stability with YPUOH loading. Due to the good antibacterial property of as-prepared YPUOH, LDPE/YUOH composite films also showed an excellent antibacterial activity (R) of 99.9% against E. coli. Furthermore, the moisture barrier property of LDPE/YPUOH composite films increased with increasing YPUOH contents. Incorporating the relatively low amount of YPUOH in LDPE resulted in the apparent enhancement in thermal stabilities, antibacterial and moisture barrier properties, which made them promising candidates as a functional filler for packaging materials.