Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Antibacterial Property of Ecklonia cava Extract against Marine Bacterial Pathogens

해양 유해세균에 대한 감태 추출물의 항균특성

  • Kim, Ji-Hoon (Department of Food Science and Technology, Pukyong National University) ;
  • Kim, Se-Bong (Department of Food Industrial Engineering, Pukyong National University) ;
  • Hwang, Hye-Jin (Department of Food Science and Technology, Pukyong National University) ;
  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University) ;
  • Lee, Myung-Suk (Department of Microbiology, Pukyong National University)
  • 김지훈 (부경대학교 식품공학과) ;
  • 김세봉 (부경대학교 식품산업공학과) ;
  • 황혜진 (부경대학교 식품공학과) ;
  • 김영목 (부경대학교 식품공학과) ;
  • 이명숙 (부경대학교 미생물학과)
  • Received : 2016.06.26
  • Accepted : 2016.09.03
  • Published : 2016.10.28
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Abstract

The object of this study was to evaluate antibacterial activity of edible seaweed extracts against marine bacterial pathogens including Edwardsiella tarda, Streptococcus iniae, Streptococcus parauberis, Vibrio anguillarum, Vibiro harveyi and Vibrio scophthalm, which are associated with human or fish infectious disease. Ecklonia cava methanolic extract showed a strong and broad spectrum antibacterial activity against marine bacterial pathogens used in this study. Among solvent-soluble fractions of the E. cava extract, the ethyl acetate (EtOAc) soluble fraction showed the strongest antibacterial activity against marine bacterial pathogens tested in this study with MIC in the range of $128-256{\mu}g/mL$. Furthermore, HPLC analysis revealed that the soluble fraction contains abundant dieckol, a phlorotannin compound, compared to other solvent soluble fractions, suggesting that phlorotannins including dieckol would be a key antibacterial agent against marine bacterial pathogens.

사람과 어류에게 감염증을 유발하는 Edwardsiella sp., Streptococcus sp. 및 Vibrio sp. 의 해양 유해세균에 대한 해조류 추출물의 항균 활성을 조사하였다. 연구에 사용된 4종의 식용 해조류 중에서 감태 MeOH 추출물이 본 연구에서 사용 된 6종의 모든 해양 유해세균에 대해 넓은 범위의 항균 활성을 나타내었다. 감태의 MeOH 추출물의 유기용매 분획 추출물들 중에서, EtOAc 분획 추출물이 가장 높은 항균활성을 나타내었으며 6종의 해양 유해세균에 대하여 $128{\mu}g/mL$에서 $256{\mu}g/mL$의 MIC 값을 나타내었다. 또한, HPLC 분석에 의해 감태 EtOAc 분획 추출물에 phlorotannin 화합물인 dieckol이 다량 존재하고 있는 것이 확인 되었다. 결론적으로, 감태 추출물의 phlorotannin 화합물이 여러 유해세균에 대한 강한 항균활성을 나타내는 것처럼, 해양 유해세균에 대해서도 강한 항균활성을 나타내는 것으로 판단된다.

Keywords

References

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