• 생약학회지
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• 제23권3호
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• pp.142-145
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• 1992

MeOH extract of twenty medicinal herbs were screened for their effects against protein kinase C (PKC) using bleb-forming assay and PKC enzyme assay. Smilax china and Sanguisorba officinalis showed potent anti-PKC activity. Campsis grandiflora and Galla Halepensis showed moderate inhibitory effect on PKC.

• 한국환경보건학회지
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• 제33권1호
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• pp.21-29
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• 2007

Paeonia lactiflora was stepwise extracted with hexane, chloroform, ethyl acetate, butanol and water. Anti-microbial activity of each extract was investigated. Methanol extract of P. lactiflora revealed anti-microbial activity against S. mutans, C. albicans, and S. aureus. Also, hexane fraction revealed anti-bacterial activity against S. mutans and ethyl acetate fraction acted as potent anti-microbial agent on C. albicans and S. aureus. The relative growth ratio(RGR) of hexane fraction of P. lactiflora against S. mutans were determined as 77.8% in concentration of 0.125 mg/ml, 98.46% in 0.25 mg/ml and 100% in 0.5 mg/ml. The ethyl acetate fraction of P. lactiflora revealed RGR against C. albicans as 52.5% in concentration of 0.125 mg/ml, 60.83% in 0.25 mg/ml and 78.33% in 0.5 mg/ml. It indicate that increasing concentration increase RGR. The measured minimal inhibitory concentration(MIC) of hexane fraction on S. mutans KCTC 5316 strain was 0.5 mg/ml and MIC of ethyl acetate fraction on C. albicans KCTC 7270 was 2.0 mg/ml. The experiment of inhibition to growth of KB roll(oral squamous cell carcinoma) result 61.9% in butanol, 76.7% in hexane extract of P. lactiflora. The hexane extract exhibit potent inhibition effect to the growth of KB cell. These results suggest that the hexane extract of Paeonia lactiflora has antimicrobial activity against S. mutans and has preventive effect to dental caries in addition to potent inhibition to KB cell growth.

• Natural Product Sciences
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• 제23권4호
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• pp.258-264
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• 2017

This study analyzed the seeds of Zizyphus jujuba var. inermis commonly used as a remedy in traditional Chinese medicine, in order to determine its various biologically active compounds. Through process 3-pentadecylcatechol, ${\rho}$-menth-8-ene, and ${\gamma}$-bisabolene were isolated and identified for the first time which are urushiol, monoterpenoidal, and sesquiterpenoidal compounds, respectively. Also, found were another sesquiterpenoidal compounds, vomifoliol, and four steroidal compounds, ${\beta}$-sitosterol, stigmasterol, stigmasta-5,23-dien-$3{\beta}$-ol, and stigmast-4-en-3-one. In addition, fourteen triterpenoidal compounds were isolated and identified. These were lupeol, betulinic acid, betulinaldehyde, alphitolic acid, 3-O-cis-${\rho}$-coumaroyl-alphitolic acid, 3-O-trans-${\rho}$-coumaroyl-alphitolic acid, 2-O-cis-${\rho}$-coumaroyl-alphitolic acid, 2-O-trans-${\rho}$-coumaroyl-alphitolic acid, zizyberanalic acid, ceanothic acid, oleanolic acid, maslinic acid, 3-O-cis-${\rho}$-coumaroyl-maslinic acid, and 3-O-trans-${\rho}$-coumaroyl-maslinic acid. The structures were identified by comparing of the spectroscopic experiments, NMR and MS, and then compared that reported data, respectively. Three extracts of water, methanol, and chloroform from the seeds showed a weak anti-proliferative effect, anti-microbial activity, and anti-oxidant effect, respectively.

• KSBB Journal
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• 제32권1호
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• pp.9-15
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• 2017

Chamomile (Matricaria chamomilla), a member of the Asteraceae family, is a well-known for medicinal plant and can be found in India and Europe. Chamomile is an effective sedative and various medical effects. But, the effects of acne treatment by chamomile were not investigated. Therefore, we assessed the anti-oxidant effects, anti-microbial activity and anti-inflammatory effects by chamomile extracts in HaCaT keratinocyte cells. Anti-oxidant effects of chamomile extracts were investigated by DPPH assay. Also, results of MTT assay was demonstrated that chamomile extracts did not have a cytotoxic effect in HaCaT cells. To assess the antimicrobial activity, we determined formation of inhibition zone of Propionibacterium acnes by extracts from chamomile. Tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) induces production of inflammatory cytokines such as interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6 and IL-8 and expression of COX-2. Chamomile extracts could inhibit TNF-${\alpha}$-induced mRNA expression levels of IL-$1{\beta}$, IL-6, IL-8 and COX-2 gene. These results demonstrated the possibility of chamomile for prevention and treatment of skin inflammatory diseases such as acne.

• 한방안이비인후피부과학회지
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• 제18권1호
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• pp.135-153
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• 2005

Herbal mixture water extract of (Phellodendron amurense, Scuellaria baiklensis, Spphora flavescens, Lithospermum erythrorhizon, Mellaphis chinesis, Alumite, Zanthoxylum schinifolium, Glycyrrhiza uralensis), which exhibit several beneficial effects including acne and skin diseases, was tested for anti-microbial activity and anti-inflammation effects. The herbal mixture extract showed antimicrobial activity against Stapylococcus epidermis, Propionibacterium acne, and Malassezia furfur. The growth of Stapylococcus epidermis, Propionibacterium acne, and Malassezia furfur was inhibited allergy and LPS induced cyclooxygenase-2(COX-2)gene expression in RAW24.7macrophage. The results indicated th ear swelling and histamine release induced by compound 48/80 were dose-dependently reduced, ranging 11-38% and 11-56%, respectively. Furthermore the extract inhibited the expression of LPS-induced COX-2 proteins and mRNAs without an appreciable cytotoxic effects on RAW264.7 cells. The cytotoxicity of the extract using M7T assay showed the cytotoxicity of 7 and 18% against L929 cell line. Based on these results, it is concluded that the herbal mixture water extract can be applied to the acne and skin diseases therapy.

• 한방안이비인후피부과학회지
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• 제18권1호
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• pp.154-171
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• 2005

Herbal mixture water extract of (Rheum coreanum Scutellaria baikalensis, Phellodendron amurese), which exhibit several beneficial effects including acne and skin diseases, was tested for anti-microbial activity and anti-inflammation effects. The herbal mixture extract showed antimicrobial activity against Stapylococcus epidermis and Propionbacterium acne. The growth of Stapylococcus epidermis and Propionibacterium acne was inhibited completely by addition of 1.0% of the extract. Also in the present study we examined the mixture extract on compound 48/80 induced allergy and LPS induced cyclooxygenase-2(COX-2) gene expression in RAW264.7 macrophage. The results indicated the ear swelling and histamine release induced by compound 48/80 were dose-dependently reduced, ranging 18-36% and 10-61%, respectively. Furthermore the extract inhibited the expression of LPS-induced COX-2 proteins and mRNAs without an appreciable cytotoxic effects on RAW264.1 cells. The cytotoxicity of the extract using MTT assay showed the cytotoxicity of 6% and 13% against L929 cell line. Based on these results, it is concluded that the herbal mixture water extract can be applied to this acne and skin diseases therapy.

• 생명과학회지
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• 제30권8호
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• pp.695-700
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• 2020

본 연구에서는 물푸레나무 껍질인 진피로부터 Syringin을 효과적으로 분리, 정제하고 항산화 및 항균 활성을 평가하여 기능성 소재로써의 가능성을 탐구하고자 하였다. Syringin은 DPPH radical 소거활성 평가에서 50 ㎍/ml의 적은 농도로도 BHT보다 높은 활성을 나타내었으며 ABTS radical 소거활성 평가에서는 모든 농도에서 BHT의 활성과 비슷한 정도의 활성을 나타냈다. PF 측정에서는 Syringin의 농도가 증가함에 따라 활성이 다소 높아지는 듯했으나 증가폭이 크지 않았고 모든 농도에서 1.2 PF 이상의 활성이 나타나 항산화력이 있다고 판단하였다. TBARs 활성 측정에서는 모든 농도에서 BHT의 활성보다 낮지만 농도 의존적으로 항산화력이 증가하는 것을 확인할 수 있었다. 그람양성, 그람음성, 진균에 대한 생육 억제 활성 평가에서는 L. monocytogenes KCTC 13064, S. aureus KCTC 1916, E. coli KCTC 2571, H. pylori HPKCTC B0150의 네 균주에 대한 생육억제환의 크기가 Syringin의 농도에 의존적으로 증가하였고 C. albicans ATCC 10231에 대해서는 생육억제환이 관찰되지 않았다. 위의 결과를 종합한 결과 Syringin의 수용성 물질의 항산화 활성은 지용성 물질의 항산화 활성보다 우수하고 진균을 제외한 그람양성 및 그람음성 균주에 대한 성장을 억제함으로써 항균 활성을 나타낸다고 판단하였다. 본 연구를 토대로 Syringin의 항산화 및 항세균 기작에 대한 연구와 다른 생리활성 작용, 인체 적용성에 대한 연구를 추가로 진행한다면 천연 유래의 안전한 기능성 소재로 활용 가능할 것으로 사료된다.

• 한국산림과학회지
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• 제100권2호
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• pp.136-141
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• 2011

본 연구는 산돌배나무 잎에서 추출한 분획물의 항암 및 항균효과를 조사하여 화장품소재로 적용 가능성을 확인하기 위하여 수행하였다. 산돌배나무 잎을 채취하여 건조시킨 후 분쇄하여 60% acetone-$H_{2}O$(6:4, v/v)로 추출하고 아세톤 가용부를 분획하여 동결건조 시켰다. 아세톤 및 물 가용부를 Sephadex LH-20으로 충진한 칼럼에서 MeOH을 용출용매로 사용하여 칼럼크로마토그래피를 실시하고 또한 MeOH를 전개용매로 사용한 MIC-gel을 이용했다. 단리된 화합물들은 silica-gel TLC로 확인하였다. 항암효과는 마우스 피부암 세포(B16F10)를 이용한 MTT assay로 측정했다. 산돌배나무 잎으로부터 얻어진 분획물의 암세포 생장 저해율은 80% 이상의 저해률을 보였다. 항균활성 실험에서 산돌배나무 잎의 분획물은 그람양성균인 Staphylococcus aureus, Staphylococcus epidermidis에 대하여 0.25 mg/disc에서 1.3 cm, 2 cm의 생육 저해환을 나타내었다. 그람음성균인 Escherichia coli 에 대하여 0.5 mg/disc에서 각 분획물들은 1.1 cm~1.5 cm의 생육 저해환을 나타내었다. 이상의 결과로 미루어 보아 산돌배나무 잎의 아세톤 분획물은 항암 및 항균효과를 가진 천연소재임을 확인할 수 있었다.

• 한국산림과학회지
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• 제97권3호
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• pp.215-220
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• 2008

최근 식물을 이용한 추출물이 화장품과 의약품에 많이 이용되고 있다. 이에 본 연구에서는 수목의 미활용 부위인 리기다소나무 내수피의 화장품소재로써 가능성을 검토하고자 연구하였다. 이를 위해 리기다소나무 내수피 추출물을 이용하여 아질산염 소거능, 항염효과, 항균효과를 분석하여 다음과 같은 결론을 얻었다. 아질산염 소거능 실험결과 100ppm에서 물가용부는 88.7%, EtOAc가용부는 99%로 대조군인 vitamin C와 유사한 소거작용을 나타내어 리기다소나무 내수피 추출물의 뛰어난 아질산염 소거능을 확인할 수 있었다. 시료의 NO 저해활성이 세포독성으로 인한 cell population의 저하에서 기인하는 것인지 관찰하기위해 MTT assay를 통해 cell의 생존율을 측정한 결과 80% 이상의 세포 생존율을 나타내어 이것을 토대로 NO 저해활성의 실험을 진행하였다. LPS로 활성화된 Raw 264.7 세포의 배양액 중에 생성된 nitrite양을 griess시약을 사용하여 NO생성 저해를 측정하였다. 물가용부와 EtOAc가용부 모든 농도에서 12시간부터 24시간까지 NO생성을 저해하였으며, 물가용부는 100ppm의 낮은 농도에서 뛰어난 NO생성 저해능을 나타내었다. 항균효과 측정 실험에서 그람양성균인 S. aureus에 대하여 물가용부 3mg/disc에서 14mm의 생육 저해환을 나타내었으며, EtOAc가용부는 3mg/disc에서 15mm의 생육 저해환을 나타내었다. 효모인 C. albicans에 대해서는 EtOAc가용부 3mg/disc에서 15mm의 생육 저해환을 나타내었으며, S. epidermidis와 그람음성균인 E. coli에서는 생육 저해환을 나타내지 않았다. 본 연구의 결과에서는 리기다소나무 내수피 추출물의 항염 및 항균효과와 천연 소재로서 화장품 산업에 활용 가능성을 확인할 수 있었다.

• 생약학회지
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• 제48권3호
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• pp.219-225
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• 2017

We investigated the anti-oxidative effect of the blueberry duke (Vaccinium corymbosum L., Ericaceae) ethanol extract in Caenorhabditis elegans model. The ethanol extract of blueberry duke showed relatively significant DPPH radical scavenging and superoxide quenching activities. To prove antioxidant activity of the extract, we checked the activities of superoxide dismutase (SOD), catalase, intracellular ROS, and oxidative stress tolerance in C. elegans. In addition, to verify if the increased stress tolerance of C. elegans by treating with the extract was due to regulation of stress-response genes, we checked SOD-3 expression using a transgenic strain. As a consequence, the blueberry duke ethanol extract increased SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Besides, blueberry duke ethanol extract-treated CF1553 worms showed higher SOD-3::GFP intensity.