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Study on Anti-inflammatory and Anti-microbial Effect of Pinus rigida Mill. inner Bark Extracts as a Cosmetic Material  

Jang, Min-Jung (Department of Cosmeceutical Science, Daegu Haany University)
Kim, Young-Hun (Department of Cosmeceutical Science, Daegu Haany University)
An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
Lee, Chang-Eeon (Department of Cosmeceutical Science, Daegu Haany University)
Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University)
Kim, Sea-Hyun (Department of Forest Genetic Resources, Korea Forest Research Institute)
Lee, Byung-Guen (Department of Forest Resources, Yeungnam University)
Lee, Do-Hyung (Department of Forest Resources, Yeungnam University)
Publication Information
Journal of Korean Society of Forest Science / v.97, no.3, 2008 , pp. 215-220 More about this Journal
Abstract
Recently, there has been a great deal of interest in the applications of plant-based extracts to both cosmetic and medicinal industries. The objective of this study was to investigate the anti-inflammatory and antimicrobial effect of P. rigida extracts by water and ethyl acetate. Anti-inflammatory and anti-microbial effect of P. rigida extracts by water and EtOAc were investigated by using nitrite scavenging ability, nitric oxide production and anti-microbial ability. In the test of nitrite scavenging ability, P. rigida extracts by water and EtOAc showed 88.7% and 99% at 100 ppm concentration, respectively. The cell viability was measured using the MTT assay at 24 hours after P. rigida extracts as shown in over 80%. Anti-inflammatory effect was examined in LPS stimulated RAW 264.7 cells. NO productions in LPS and P. rigida extracts stimulated group were decreased in a concentration and were dependent on time as compared with LPS stimulated. The water extracts showed the highest inhibition at the 100 ppm concentration. In anti-microbial activity test, the water extract with 3.0 mg/disc resulted in the clear zone of 14 mm, and ethyl acetate with that of 15 mm for Staphylococcus aureus. However, P. rigida extracts didn't show any growth inhibitory effect on Esherichia coli. These results indicate that the extracts of P. rigida have anti-inflammatory activities as a cosmeceuticals.
Keywords
Pinus rigida Anti-inflammatory and anti-microbial; NO production; LPS;
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