• Journal of Nutrition and Health
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• 제46권2호
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• pp.126-136
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• 2013

This study was conducted in order to investigate the association between hypertension and oxidative stress-related parameters and to evaluate these parameters in subclinical hypertensive patients and normotensive subjects living in Korea. We attempted to determine whether oxidative stress-related parameters would differ between two groups of 227 newly-diagnosed, untreated (systolic blood pressure (BP) ${\geq}$ 130 mmHg and diastolic BP ${\geq}$ 85 mmHg) and 130 normotensive subjects (systolic BP < 120 mmHg and diastolic BP < 80 mmHg). General characteristics of the subjects were collected using a simple questionnaire. From subjects' blood, degree of DNA damage in lymphocytes, the activities of erythrocyte superoxide dismutase, catalase, and glutathione peroxidase, level of plasma total radical-trapping antioxidant potential (TRAP), glutathione, and anti-oxidative vitamins, as well as plasma lipid profiles and conjugated diene (CD) were analyzed. Evaluation of the associations of oxidative stress-related parameters with blood pressure of the subjects was performed using Pearson partial correlation and multivariate logistic regression analysis after adjusting for confounding factors. Several oxidative stress-related parameters were higher in subclinical hypertensive patients than in normotensive subjects. Plasma levels of ${\alpha}$-tocopherol, ${\beta}$-carotene, TRAP, and activity of GSH-px were significantly lower in subclinical hypertensive patients than in normotensive subjects. Increased levels of DNA damage, lipid peroxidation, triglyceride, total cholesterol, and LDL-cholesterol were observed in subclinical hypertensive patients. These results confirm an association between blood pressure and oxidative stress-related parameters and suggest that the pathogenic role of oxidative stress in hypertension might be significant.

• 동의생리병리학회지
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• 제28권5호
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• pp.537-547
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• 2014

The object of this study was to observe the effect of Banhasasim-tang (BHSST) on the HCl/EtOH-induced gastric ulcer in mice. Three different dosages of BHSST extract (400, 200 and 100 mg/kg) were once orally administered 1 hr before HCl/EtOH mixture treatment. One hour after HCl/EtOH mixture single oral treatment, the changes on the gross hemorrhagic lesion scores, fundic histopathology, gastric nitrate/nitrite contents, lipid peroxidation and antioxidant defense system (catalase and superoxide dismutase (SOD) activities) were observed, and compared with that of ranitidine 100 mg/kg. As results of all three different dosages of BHSST extract treatment in the HCl/EtOH-induced gastric ulcer mice, significant and dose-dependent decreased gastric damages. BHSST extracts also increased gastric nitrate/nitrite contents and strengthened the antioxidant defense systems, and increased the activities of catalase and SOD, respectively. BHSST extracts 200 mg/kg showed similar anti-ulcerative effect as compared with ranitidine 100 mg/kg, in this experiment. BHSST has favorable protective effects against to the HCl/EtOH-induced gastric damages, through the strengthening of the body antioxidant defense systems. These gastroprotective effects of BHSST against HCl/EtOH-induced gastric ulcer considered as results of complicated synergic effects of their 8 kinds of herbal components, but exact synergic or individual herbal effects are difficult to discuss in this study. Therefore, more detail synergic effects between 8 kinds of individual herbal component of BHSST should be tested with screening of active anti-inflammatory chemical ingredients, in future.

• Preventive Nutrition and Food Science
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• 제13권4호
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• pp.269-275
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• 2008

Dandelion (Taraxacum officinale) has been widely used as an anti-inflammatory agent in oriental medicine. In the current study, we investigated the protective effect, and the possible mechanism, of dandelion extracts against ethanol-induced acute hepatotoxicity in C57BL/6 mice. Dandelion water and ethanol extract was administered at 2 g/kg body weight (BW) once daily for 7 consecutive days, whereas control and ethanol groups received water by gavage. Ethanol (50% ethanol; 6 g/kg BW) was administered 12 hr before sacrificing the mice in order to generate liver injury. Significantly increased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities as well as liver triglyceride (TG) and cholesterol levels were attenuated by dandelion supplementation. In addition, dandelion extracts not only enhanced alcohol dehydrogenase (ADH) and anti-oxidative enzyme activities, but reduced lipid peroxidation. Cytochrome P450 2E1 (CYP 2E1), one of the critical enzymes xenobiotic metabolism, expression was lower with ethanol treatment but restored by dandelion supplementation. These results were confirmed by improved histopathological changes in fatty liver and hepatic lesions induced by ethanol. In conclusion, dandelion could protect liver against ethanol administration by attenuating of oxidative stress and inflammatory responses.

• 농업생명과학연구
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• 제45권6호
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• pp.183-191
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• 2011

In vitro 항산화 활성을 갖는 율피 추출물의 향장 소재 특성을 조사하였다. 율피로부터의 다양한 추출물 중 총 페놀성 화합물 함량은 60% 메탄올 추출물 및 에틸아세테이트 분획물이 각각 164.82 및 191.14 mg/g으로 가장 높은 것으로 나타났다. 또한, 상기 두 분획물에 의한 in vitro 항산화 활성(DPPH radical scavenging activity, lipid peroxidation 저해)은 농도 의존적으로 증가한다는 것을 확인하였다. 상기 두 분획물은 UV-B(290-320 nm) 영역에서 강한 UV 흡수도를 보여주었다. 특히, 율피 유래 두 분획물은 $1000{\mu}g/mL$에서 42.88 및 33.02%의 elastase 저해 활성을 나타내어 우수한 주름 개선 특성을 가지는 것을 보여주었다. 아울러, 율피로부터의 60% 메탄올 추출물 및 에틸아세테이트 분획물은 피부 상재균에 대하여서도 항균 활성을 지니는 것으로 나타났다. 이러한 결과들은 율피에서 추출될 수 있는 물질이 향장 산업소재로서 활용될 수 있는 가능성을 제시하는 것으로 판단된다.

• KSBB Journal
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• 제18권4호
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• pp.282-288
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• 2003

본 연구에서는 항염증 및 항산화 활성을 갖는 유용한 물질 을 검색하기 위해 40여 가지 생약재를 에탄올 추출하여 H HAase 억제 및 라디칼 소거 활성을 측정하였다 .. HAase 억제 활성을 측정한 결과, I mg/mL의 농도에서 황기, 두충, 오미 자, 황금, 오가피, 목과, 목단피, 산사가 50% 이상의 저해활 성을 나타내었고, 이들에 대하여 n-hexane, chloroform, ethyl a acetate, water로 순차적으로 용매분획하여 활성을 측정한 결 과, 모든 시료의 ethyl acetate와 water 분획에서 높은 활성을 보였다 항산화 활성은 DPPH 라디칼에 대한 전자공여능과 F Fenton 반응에 의해 발생한 hydroxyl radical에 대한 소거능으 로 평가하였다 .. DPPH 라디칼에 대한 전자공여능은 복분자, 목단피, 백작약, 차전자, 마가목 에탄올 추출물이 I mg{mL 놓도에서 90% 이상으로 대조군으로 이용한 BHA와 Q - -tocopherol과 유사한 활성을 나타내었다 .. 2-deoxyribose 산화 법에 따른 hydroxyl 라디칼 소거활성에서 대부분의 에탄올 추출물 시료에서 모두 높은 활성을 나타내었다. 활성올 갖는 시료에 대하여 지질과산화 억제 활성을 측정한 결과 linoleic a acid와 phosphatidylcholine liposome 기질 모두에 대하여 오미 자 추출물은 가장 높은 억제 활성을 나타내었다.

• 대한한의학방제학회지
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• 제16권2호
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• pp.183-191
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• 2008

Acetaminophen (N-acety1-p-aminophenol, paracetamol) is widely used as an over-the-counter analgesic and antipyretic drug. Intake of a over dose of acetaminophen may result in severe hepatic necrosis. In this study, we investigated the liver damage in mice using single dose (300 mg/kg) of acetaminophen and the possible protective effects of administration (50-200 mg/kg body weight) of SB-Ex on acetaminophen-induced liver damage in mice. The alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities were determined in the plasma of mice. The effect of SB-Ex on lipid peroxidation product thiobarbituric reacting substances (TBARS) and some antioxidant enzymes superoxide dismutase (SOD), catalase, d-aminolevulinate dehydratase (${\sigma}$-ALA-D) activities, and gluthathione peroxidase (GPx), were also evaluated in the mouse liver homogenate. Acetaminophen caused liver damage as evident by statistically significant increased in plasma activities of AST and ALT. There were general statistically significant losses in the activities of SOD, catalase, ${\sigma}$-ALA-D, and GPx and an increase in TBARS in the liver of acetaminophen-treated group compared with the control group. However, SB-Ex was able to counteract these effects. These results suggest that SB-Ex can act as hepatoprotectives against acetaminophen toxicity and is a good candidate for further evaluation as an effective chemotherapeutic agent.

• 생명과학회지
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• 제15권5호
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• pp.819-825
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• 2005

항산화물질인 glutathione (${\gamma}$-L-glutamyl-cysteinyl-glycine) 고함유 효모 Saccharomyces cerevisiae FF-8에서 생산된 glutathione을 함유한 세포 추출액의 항산화 활성을 in vitro 과산화지질 실험계인 DPPH($a,{\;}\acute{a}-diphenyl-\beta-picrylhydrazyl$)법, linoleic acid를 이용한 ferric thiocyanate법과 TBA법 및 microsome 생체막 지질 과산화물 생성정도의 TBARS법으로 측정하였다. YM 최적 배지에서 생산된 glutathione 농도는 $204\mug/ml$로 YM 기본배지에서 생산된 glutathione 농도 $74\mug/ml$보다 2.76배 증가하였다. 본 실험에서는 YM 기본배지와 최적배지에서 S. cerevisiae FF-8이 생산하는 glutathione 함량에 따른 항산화 활성을 비교하였다. DPPH 측정법에서는 짙은 자색의 탈색되는 정도로 나타내는 전자 공여능이 glutathione 함량이 높은 최적 생산배지에서 S. cerevisiae FF-8가 생산하는 glutathione고함유 세포 추출액에서 대조구인 $ 0.05\%BHT $와 비슷한 수준으로 항산화 활성이 높게 나타났다. 각 조직 microsome을 이용한 생체막 지질 과산화 억제정도는 최적 생산배지에서 전체적으로 높게 나타났으며, 간장 $60.98\%$, 신장 $56.43\%$, 심장 $52.91\%$, 뇌 $52.13\%$, 고환$45.57\%$ 및 비장 $42.95\%$순으로 나타났다 Linoleic acid 산화 실험계를 이용한 ferric thiocyanate법에서는 최적 생산배지가 반응 7일째까지 대조구에 비해 강한 항산화 활성을 보였으며, TBA법에서는 반응 5일째까지 최적 생산배지가 YM 배지보다는 높은 항산화 활성을 나타내었다 이상의 결과에서 Saccharomyces cerevisiae FF-8 균주가 glutathione을 생산하는 최적배지 조건에서 생산된 glutathione 고함유 세포 추출액은 in vitro 항산화 실험계인 DPPH radical scavenging activity, ferric thiocyanate and TBARS 측정에서 항산화 활성을 나타내는 생리활성 성분을 지닌 것으로 나타나 천연 항산화제로서의 사용 가능성을 시사하였다.

• Food Science and Biotechnology
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• 제18권2호
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• pp.449-455
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• 2009

The antioxidant properties of water extracted polysaccharides (PW), 1%(w/v) NaOH extracted polysaccharides (PN1), and 5%(w/v) NaOH extracted polysaccharides (PN5) were assessed in this paper. PW showed good capability of scavenging $H_2O_2$, anti-lipid peroxidation, reduction power, and scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH). The maximum values were all observed at the dose of 1 mg/mL sample. As far as the antioxidant activities of PN1 were concerned, the capability of scavenging superoxide radical, chelating metal, and total antioxidation showed higher than those of PN5. When polysaccharide concentration was 1 mg/mL, the 3 index described above were attached 71.8%, 51.6%, and $446.3{\mu}M$ Trolox equivalent, respectively. With respect to PN5, higher capabilities of scavenging hydroxyl free radicals were obtained while the peak of 84.8% was observed at the concentration of 1.0 mg/mL. Moreover, the concentration-dependent influences were characterized in all cases.

• 대한중풍순환신경학회지
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• 제10권1호
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• pp.8-19
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• 2009

Scutellaria Radix, originated from Scutellaria baicalensis Georgi, is one of the most important medicine in traditional Oriental medicine, and possesses anti-bacterial activity and sedative effects, can be applied in the treatment of a range of conditions including diarrhea and hepatitis. It is reported that chronic global ischemia induces neuronal damage in selective, vulnerable regions of the brain, especially the hippocampus and cerebral cortex. In the present study, to investigate the effect of Scutellaria Radix extract on cerebral disease, the changes of regional cerebral blood flow and pial arterial diameter on ischemia/reperfusion state was determinated by Laser-Doppler Flowmetry and some parameters concerned with oxidative stress also measured. When SRe were administered for five days with the concentration of 100 mg/kg, GSH activity significantly increased. But SRe administeration showed no significant change in lipid peroxidation. When the activities of CAT, Cu, Zn-SOD and GSH were measured, CAT and GSH were activated by SRe administration. When 1 and 3 ㎍/㎖ SRe was applied to the neuronal cell cultures, the quantities of LDH was significantly reduced when compared with cultures treated only with NMDA. Through this study, it can be concluded that the ischemia/reperfusion induced brain stress may have contributed to cerebral damage in rats, and the present study provides clear evidence for the beneficial effect of SRe on ischemia induced brain injury.

• Toxicological Research
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• 제28권4호
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• pp.241-248
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• 2012

Exposure of cells to ultraviolet B (UVB) radiation can induce production of free radicals and reactive oxygen species (ROS), which damage cellular components. In addition, these agents can stimulate the expression of matrix metalloproteinase (MMP) and decrease collagen synthesis in human skin cells. In this study, we examined the anti-photoaging effects of extracts of Tetraselmis suecica (W-TS). W-TS showed the strongest scavenging activity against 2,2-difenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals, followed by superoxide anions from the xanthine/xanthine oxidase system. We observed that the levels of both intracellular ROS and lipid peroxidation significantly increased in UVB-irradiated human skin fibroblast cells. Furthermore, the activities of enzymatic antioxidants (e.g., superoxide dismutase) and the levels of non-enzymatic antioxidants (e.g., glutathione) significantly decreased in cells. However, W-TS pretreatment, at the maximum tested concentration, significantly decreased intracellular ROS and malondialdehyde (MDA) levels, and increased superoxide dismutase and glutathione levels in the cells. At this same concentration, W-TS did not show cytotoxicity. Type 1 procollagen and MMP-1 released were quantified using RT-PCR techniques. The results showed that W-TS protected type 1 procollagen against UVB-induced depletion in fibroblast cells in a dose-dependent manner via inhibition of UVB-induced MMP-1. Taken together, the results of the study suggest that W-TS effectively inhibits UVB-induced photoaging in skin fibroblasts by its strong anti-oxidant ability.