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Ameliorating Effects of Banhasasim-tang Extrat on the HCl/EtOH-induced Gastric Mucosa Damages in Mice  

Lee, Eun Jung (Department of Internal Medicine, College of Korean Medicine, Daegu Hanny University)
Kwak, Min Ah (Department of Internal Medicine, College of Korean Medicine, Daegu Hanny University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.5, 2014 , pp. 537-547 More about this Journal
Abstract
The object of this study was to observe the effect of Banhasasim-tang (BHSST) on the HCl/EtOH-induced gastric ulcer in mice. Three different dosages of BHSST extract (400, 200 and 100 mg/kg) were once orally administered 1 hr before HCl/EtOH mixture treatment. One hour after HCl/EtOH mixture single oral treatment, the changes on the gross hemorrhagic lesion scores, fundic histopathology, gastric nitrate/nitrite contents, lipid peroxidation and antioxidant defense system (catalase and superoxide dismutase (SOD) activities) were observed, and compared with that of ranitidine 100 mg/kg. As results of all three different dosages of BHSST extract treatment in the HCl/EtOH-induced gastric ulcer mice, significant and dose-dependent decreased gastric damages. BHSST extracts also increased gastric nitrate/nitrite contents and strengthened the antioxidant defense systems, and increased the activities of catalase and SOD, respectively. BHSST extracts 200 mg/kg showed similar anti-ulcerative effect as compared with ranitidine 100 mg/kg, in this experiment. BHSST has favorable protective effects against to the HCl/EtOH-induced gastric damages, through the strengthening of the body antioxidant defense systems. These gastroprotective effects of BHSST against HCl/EtOH-induced gastric ulcer considered as results of complicated synergic effects of their 8 kinds of herbal components, but exact synergic or individual herbal effects are difficult to discuss in this study. Therefore, more detail synergic effects between 8 kinds of individual herbal component of BHSST should be tested with screening of active anti-inflammatory chemical ingredients, in future.
Keywords
Banhasasim-tang Extrat; Gastric Ulcer; Antioxidant effect; Ranitidine;
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1 Yu, L., Jiang, B.P., Luo, D., Shen, X.C., Guo, S., Duan, J.A., Tang, Y.P. Bioactive components in the fruits of Ziziphus jujuba Mill. against the inflammatory irritant action of Euphorbia plants. Phytomedicine. 19(3-4):239-244, 2012.   DOI
2 Mori, J., Hayashi, T., Iwashima, M., Matsunaga, T., Saito, H. Effects of plastoquinones from the brown alga Sargassum micracanthum and a new chromene derivative converted from the plastoquinones on acute gastric lesions in rats. Biol Pharm Bull. 29(6):1197-1201, 2006.   DOI   ScienceOn
3 Kawano, S., Tsuji, S. Role of mucosal blood flow: a conceptional review in gastric mucosal injury and protection. J Gastroenterol Hepatol. 15: D1-6, 2000.
4 Hiramoto, K., Ohkawa, T., Oikawa, N., Kikugawa, K. Is nitric oxide (NO) an antioxidant or a prooxidant for lipid peroxidation? Chem Pharm Bull (Tokyo). 51: 1046-1050, 2003.   DOI
5 Ziang Wei, Gu Wujun, Zhou Chun xiang. Influence of Banxia Xiexin Decoction on SOD and MDA in rats with chronic gastritis combined with Hp infection. Tianjin Journal of Traditional Chinese Medicine. 20(5):27-30, 2003.
6 Yoshio Kase, Mitsutoshi Yuzurihara, Susumu Iizuka, Atsusi Ishige, Yasuhiro Komatsu. The effects of Hange-shashin-to on gastric function in comparison with Sho-saiko-to. Biol. PHARM. BULL. 20(11):1155-1159, 1997.   DOI
7 Han, M.H., Yang, X.W., Zhong, G.Y., Zhang, M. Bioactive constituents inhibiting TNF-alpha production in fresh rhizome of Pinellia ternata. Zhongguo Zhong Yao Za Zhi. 32: 1755-1759, 2007.
8 Ramesh. T., Kim, S.W., Hwang, S.Y., Sohn, S.H., Yoo, S.K., Kim, S.K. Panax ginseng reduces oxidative stress and restores antioxidant capacity in aged rats. Nutr Res. 32(9):718-726, 2012.   DOI   ScienceOn
9 Li, Y.J., Chen, J., Li, Y., Li, Q., Zheng, Y.F., Fu, Y., Li, P. Screening and characterization of natural antioxidants in four Glycyrrhiza species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry. J Chromatogr A. 1218(45):8181-8191, 2011.   DOI   ScienceOn
10 Kubra, I.R., Rao, L.J. An impression on current developments in the technology, chemistry, and biological activities of ginger (Zingiber officinale Roscoe). Crit Rev Food Sci Nutr. 52(8):651-688, 2012.   DOI
11 Cho, J.Y., Kim, A.R., Park, M.H. Lignans from the rhizomes of Coptis japonica differentially act as anti-inflammatory principles. Planta Med. 67(4):312-316, 2001.   DOI   ScienceOn
12 Green. L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem. 126(1):131-138, 1982.   DOI   ScienceOn
13 El-Missiry, M.A., El-Sayed, I.H., Othman, A.I. Protection by metal complexes with SOD-mimetic activity against oxidative gastric injury induced by indomethacin and ethanol in rats. Ann Clin Biochem. 38(Pt 6):694-700, 2001.   DOI   ScienceOn
14 Baliga, M.S., Haniadka, R., Pereira, M.M., Thilakchand, K.R., Rao, S., Arora, R. Radioprotective effects of Zingiber officinale Roscoe (ginger): past, present and future. Food Funct. 3(7):714-723, 2012.   DOI
15 Minami, M., Yoshikawa, H. A simplified assay method of superoxide dismutase activity for clinical use. Clinica Chimica Acta, 92(3):337-342, 1979.   DOI   ScienceOn
16 Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248-254, 1976.   DOI   ScienceOn
17 Miederer, S.E. Will anti-ulcer drugs differ only in their side effects ?. Fortschr Med. 104(3):918-920, 1986.
18 Yesilada, E., Gurbuz, I. A compilation of the studies on the antiulcerogenic effects of medicinal plants. In: Recent Progress in Medicinal Plants, vol. II: Phytochemistry and Pharmacology (Singh S, Singh VK, Govil JN, eds). Houston: SCI Tech Publishing LLC,. pp 111-174, 2003.
19 Santos, F.A., Rao, V.S. 1,8-cineol, a food fl avoring agent, prevents ethanol-induced gastric injury in rats. Dig Dis Sci. 46: 331-337, 2001.   DOI   ScienceOn
20 Li, J., Takeda, H., Inazu, M., Hayashi, M., Tsuji, H., Ikoshi, H., etc. Protective effects of Hange-shashin-to on water immersion restraint stress-induced gastric ulcers. Methods and findings in experimental and clinical pharmacology. 20(1):31-37, 1998.   DOI
21 Wang, Q.G., Li, Y.H., Niu Xin., et al. Effects of Banxia Xiexin decoction and its decomposed recipe on epidermic growth factor in rats with chronic gastric ulcer. Chinese Journal of Integrated Traditional and Western Medicine. 8(3):137, 2001.
22 Yuan, Y., Shuai, L., Chen, S., Huang, L., Qin, S., Yang, Z. Flavonoids and antioxidative enzymes in temperature- challenged roots of Scutellaria baicalensis Georgi. Z Naturforsch C. 67(1-2):77-85, 2012.   DOI
23 Naito, T., Itoh, H., Yasunaga, F., Takeyama, M. Hange-shashin-to raises levels of somatostatin, motilin, and gastrin in the plasma of healthy subjects. Biol Pharm Bull. 25(3):327-331, 2002.   DOI   ScienceOn
24 Naito, T., Itoh, H., Takeyama, M. Some gastrointestinal function regulatory Kampo medicines have modulatory effects on human plasma adrenocorticotropic hormone and cortisol levels with continual stress exposure. Biol Pharm Bull. 26(1):101-104, 2003.   DOI   ScienceOn
25 Naito, T., Itoh, H., Takeyama, M. Comparison of the effects of hange-shashinto and rikkunshi-to on human plasma calcitonin gene-related peptide and substance P levels. Biol Pharm Bull. 26(8):1104-1107, 2003.   DOI   ScienceOn
26 Park, K.H., Choi, S.W., Park, S.J., Joo, J.C., Park, H.S. Three case reports of peptic ulcer patients treated with Banhasasim-tang. Korean J. Oriental Physiology & Pathology. 26(6):940-946, 2012.   과학기술학회마을
27 Grover, J.K., Adiga, G., Vats, V., Rathi, S.S. Extracts of Benincasa hispida prevent development of experimental ulcers. J Ethnopharmacol. 78(2-3):159-164, 2001.   DOI   ScienceOn
28 Evans, R.C., Diplock, A.T. Laboratory techniques in biochemistry and molecular biology. In Techniques in Free Radical Research (Burtin RH and Knippenberg PH, Eds). Amsterdam: Elsevier. pp 199-201, 1991.
29 Morais, T.C., Pinto, N.B., Carvalho, K.M., Rios, J.B., Ricardo, N.M., Trevisan, M.T., Rao, V.S., Santos, F.A. Protective effect of anacardic acids from cashew (Anacardium occidentale) on ethanol-induced gastric damage in mice. Chem Biol Interact. 183(1):264-269, 2010.   DOI   ScienceOn
30 Ohkawa, H., Ohishi, N., Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95(2):351-358, 1979.   DOI   ScienceOn
31 Ku, S.K., Seo, B.I., Park, J.H., Park, G.Y., Seo, Y.B., Kim, J.S., Lee, H.S., Roh, S.S. Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity. World J Gastroenterol. 15(38):4799-4805, 2009.   DOI   ScienceOn
32 Kwiecień, S., Brzozowski, T., Konturek, P.C.h, Konturek, S.J. The role of reactive oxygen species in action of nitric oxide-donors on stress-induced gastric mucosal lesions. J Physiol Pharmacol. 53(4 Pt 2):761-773, 2002.
33 Oyagi, A., Ogawa, K., Kakino, M., Hara, H. Protective effects of a gastrointestinal agent containing Korean red ginseng on gastric ulcer models in mice. BMC Complement Altern Med. 10: 45, 2010.   DOI   ScienceOn
34 Mizui, T., Sato, H., Hirose, F., Doteuchi, M. Effect of antiperoxidative drugs on gastric damage induced by ethanol in rats. Life Sci. 41(6):755-763, 1987.   DOI   ScienceOn
35 Ahn, B.O., Ko, K.H., Oh, T.Y., Cho, H., Kim, W.B., Lee, K.J., Cho, S.W., Hahm, K.B. Efficacy of use of colonoscopy in dextran sulfate sodium induced ulcerative colitis in rats: the evaluation of the effects of antioxidant by colonoscopy. Int J Colorectal Dis. 16(3):174-181, 2001.   DOI   ScienceOn
36 Jang, M.S., Lim, S.W. Experimental Study for Effect of Banhasasim-tang on Mice with Reflux Esophagitis. Korean J. Orient. Int. Med. 34(4):362-374, 2013.   과학기술학회마을
37 Szabo, S., Brown, A. Prevention of ethanol-induced vascular injury and gastric mucosal lesions by sucralfate and its components: possible role of endogenous sulfhydryls. Proc Soc Exp Biol Med. 185(4):493-497, 1987.   DOI
38 Park, J.W., Ryu, B., Yeo, I., Jerng, U.M., Han, G., Oh, S., Lee, J., Kim, J. Banha-sasim-tang as an herbal formula for the treatment of functional dyspepsia: a randomized, double-blind, placebo-controlled, two-center trial. East-West medical research institute journal. 11: 83, 2010.
39 Lee, J.S., Yoon, S.H.m., Kim, J.S., Ryu, B.H. Effect of Banhasasimtang granule on gastric emptying in rats. Korean J Orient Int Med. 27(2):471-479, 2006.   과학기술학회마을
40 Naito, Y., Yoshikawa, T., Matsuyama, K., Yagi, N., Arai, M., Nakamura, Y., Nishimura, S., Yoshida, N., Kondo, M. Effects of oxygen radical scavengers on the quality of gastric ulcer healing in rats. J Clin Gastroenterol. 21: S82-86, 1995.   DOI