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Evaluation of the Potential of Cellobiose as a Material for Whitening Cosmetics based on Autophagy and Melanin Production Efficacy in Melanocytes (셀로비오스의 미백화장품 소재 가능성 평가를 위한 멜라닌 세포에서 자가포식 및 멜라닌 생성 효능 연구)

  • Byungsun, Cha;Seok ju, Lee;Sofia, Brito;So Young, Jung;So Min, Lee;Lei, Lei;Sang Hun, Lee;Zubaidah, Al-Khafaji;Bum-Ho, Bin;Byeong-Mun, Kwak;Hyojin, Heo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.48 no.4
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    • pp.365-372
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    • 2022
  • Cellobiose is a dissacharide constituted by two glucose units joined by a β-('1,4') glycosidic bond that is produced by the decomposition of cellulose. This product exists naturally in plants and has been utilized in different industries as a food sweetener, and as a cosmetic and pharmaceutical material. In this study, the potential of cellobiose as a whitening cosmetic product was evaluated by analyzing autophagy induction and the inhibition of melanin production. A cytotoxicity test conducted in the human melanin-producing cell line MNT-1 with increasing concentrations of cellobiose revealed that this compound did not cause cytotoxicity at 20 mg/mL or less. Based on this, autophagy was firstly evaluated by immunostaining with the autophagy marker microtubule-associated protein 1 light chain 3 (LC3) after treatment with 20 mg/mL of cellobiose. The subsequent confocal microscopy analysis revealed an increase in LC3 puncta, indicating induction of autophagy. In addition, autophagy was further confirmed by western blot analysis, which demonstrated that cellobiose converted LC3-I to LC3- ∏ in a concentration- and time-dependent manners. An analysis of melanin contents after cellobiose treatment at a concentration of 20 mg/mL during 7 days revealed that melanin production was reduced by more than 50%. Additionally, the expression levels of melanogenesis-related proteins TYR and TYRP1 were markedly decreased after cellobiose treatment. Based on these studies, a cosmetic cream formulation containing cellobiose was prepared and the change in formulation was tested for 4 weeks, and it was confirmed that the appearance changed to liquid form at high temperature, but the pH did not change. In conclusion, the present research demonstrated that cellobiose activates autophagy and inhibits melanin production, and showed the potential of this product as a material for whitening cosmetics.

Development and Feasibility Study of the Nature of Science Instrument for Elementary School Students (초등학생용 과학의 본성 검사 도구 개발 및 타당성 검토)

  • Park, Jaehyeon;Park, Jaeyong
    • Journal of Korean Elementary Science Education
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    • v.41 no.4
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    • pp.701-724
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    • 2022
  • In this study, the Nature of Science (NOS) instrument for elementary school students in the form of open questionnaires was developed specifically to reveal elementary school students' perceptions of the NOS, and its validity and effectiveness were investigated. To develop a NOS instrument for elementary school students, problems that may occur when applying the existing NOS instruments to elementary school students were analyzed and based on this, the development direction of the NOS instrument was established. In addition, after selecting seven NOS types suitable for the level of elementary school students, the preliminary instrument was produced by modifying and supplementing the items in the existing instruments for each type or by developing new items. Finally, the NOS instrument consisting of eight questions was developed by adding one question asking for a comprehensive understanding of science to seven questions related to each type of NOS after a content validity test of the science education expert group. To verify the practical effect of the developed instrument, pre- and post-tests were conducted on 50 students in two classes of sixth grade at two elementary schools in Seoul: 'existing instrument → development instrument' in one class, and 'development instrument → existing instrument' in the other class. The collected data were then compared and evaluated through summary content analysis and analyzed by executing the Wilcoxon signed-rank test. As a result of comparing and analyzing students' responses to the existing NOS instrument and the developed NOS instrument, students' perspectives on the NOS were more diverse when using the developed instrument, and the level of error in the response caused by misinterpreting the intention of the question was reduced. In addition, when using the developed instrument, the responses of the majority of students at a statistically significant level changed more specifically. In this study, the implications for the development of NOS instruments suitable for elementary school students were discussed based on these results.

On Vortex Reduction Characteristics of Pump Sump Circulating Water Intake Basin of Power Plant Using Hydraulic Experiment (수리실험을 이용한 발전소의 순환수 취수부 흡입수조의 와류저감에 관한 연구)

  • Eom, Junghyun;Lee, Du Han;Kim, Hung Soo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.42 no.6
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    • pp.815-824
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    • 2022
  • Among the main facilities of the power plant, the circulating water used for cooling the power generation system is supplied through the Circulation Water Intake Basin (CWIB). The vortexes of various types generated in the Pump Sump (PS) of CWIB adversely affect the Circulation Water Pump (CWP) and pipelines. In particular, the free surface vortex accompanied by air intake brings about vibration, noise, cavitation etc. and these are the causes of degradation of CWP performance, damage to pipelines. Then power generation is interrupted by the causes. Therefore, it is necessary to investigate the hydraulic characteristics of CWIB through the hydraulic model experiment and apply an appropriate Anti Vortex Device (AVD) that can control the vortex to enable smooth operation of the power plant. In general, free surface vortex is controlled by Curtain Wall (CW) and the submerged vortex is by the anti vortex device of the curtain wall. The detailed specifications are described in the American National Standard for Pump Intake Design. In this study, the circulating water intake part of the Tripoli West 4×350 MW power plant in Libya was targeted, the actual operating conditions were applied, and the vortex reduction effect of the anti vortex device generated in the suction tank among the circulating water intake part was analyzed through a hydraulic model experiment. In addition, a floor splitter was basically applied to control the submerged vortex, and a new type of column curtain wall was additionally applied to control the vortex generated on the free surface to confirm the effect. As a result of analyzing the hydraulic characteristics by additionally applying the newly developed Column Curtain Wall (CCW) to the existing curtain wall, we have found that the vortex was controlled by forming a uniform flow. In addition, the vortex angle generated in the circulating water pump pipeline was 5° or less, which is the design standard of ANSI/HI 9.8, confirming the stability of the flow.

Analysis of the effect of long-term water supply improvement by the installation of sand dams in water scarce areas (물부족 지역에서 샌드댐 설치에 의한 장기 물공급 개선 효과 분석)

  • Chung, Il-Moon;Lee, Jeongwoo;Lee, Jeong Eun;Kim, Il-Hwan
    • Journal of Korea Water Resources Association
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    • v.55 no.12
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    • pp.999-1009
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    • 2022
  • The Chuncheon Mullori area is an underprivileged area for water welfare that does not have a local water supply system. Here, water is supplied to the village by using a small-scale water supply facility that uses underground water and underground water as the source. To solve the problem of water shortage during drought and to prepare for the increasing water demand, a sand dam was installed near the valley river, and this facility has been operating since May 2022. In this study, in order to evaluate the reliability of water supply when a sand dam is assumed during a drought in the past, groundwater runoff simulation results using MODFLOW were used to generate inflow data from 2011 to 2020, an unmeasured period. After performing SWAT-K basin hydrologic modeling for the watershed upstream of the existing water intake source and the sand dam, the groundwater runoff was calculated, and the relative ratio of the monthly groundwater runoff for the previous 10 years to the monthly groundwater runoff in 2021 was obtained. By applying this ratio to the 2021 inflow time series data, historical inflow data from 2011 to 2020 were generated. As a result of analyzing the availability of water supply during extreme drought in the past for three cases of demand 20 m3/day, 50 m3/day, and 100 m3/day, it can be confirmed that the reliability of water supply increases with the installation of sand dams. In the case of 100 m3/day, it was analyzed that the reliability exceeded 90% only when the existing water intake source and the sand dam were operated in conjunction. All three operating conditions were evaluated to satisfy 50 m3/day or more of demand based on 95% reliability of water supply and 30 m3/day or more of demand based on 99% of reliability.

A study on the rainfall management target considering inter-event time definition (IETD) (무강우 지속시간(IETD)을 고려한 빗물관리 목표량 설정 방안 연구)

  • Baek, Jongseok;Kim, Jaemoon;Park, Jaerock;Lim, Kyoungmo;Shin, Hyunsuk
    • Journal of Korea Water Resources Association
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    • v.55 no.8
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    • pp.603-611
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    • 2022
  • In urban areas, the impermeable area continues to increase due to urbanization, which interferes with the surface penetrating and infiltrating of rainwater, causing most rainwater runoff to the surface, deepening the distortion of water circulation. Distortion of water circulation affects not only flood disasters caused by rainfall and runoff, but also various aspects such as dry stream phenomenon, deterioration of water quality, and destruction of ecosystem balance, and the Ministry of Environment strongly recommends the use of Low Impact development (LID) techniques. In order to apply the LID technique, it is necessary to set a rainwater management target to handle the increase in outflow after the development of the target site, and the current standard sets the rainwater management target using the 10-year daily rainfall. In this study, the difference from the current standards was analyzed through statistical analysis and classification of independent rainfall ideas using inter-event time definition (IETD) in setting the target amount of rainwater management to improve water circulation. Using 30-year rainfall data from 1991 to 2020, methods such as autocorrelation coefficient (AC) analysis, variation coefficient (VC) analysis, and annual average number of rainfall event (NRE) analysis were applied, and IETD was selected according to the target rainfall period. The more samples the population had, the more IETD tended to increase. In addition, by analyzing the duration and time distribution of independent rainfall according to the IETD, a plan was proposed to calculate the standard design rainfall according to the rainwater management target amount. Therefore, it is expected that it will be possible to set an improved rainwater management target amount if sufficient samples of independent rainfall ideas are used through the selection of IETD as in this study.

A Study on the Korea Future Internet Promotion Plan for Cyber Security Enhancement (사이버 보안 강화를 위한 한국형 미래 인터넷 추진 방안에 관한 연구)

  • Lim, Gyoo-Gun;Jin, Hai-Yan;Ahn, Jae-Ik
    • Informatization Policy
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    • v.29 no.1
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    • pp.24-37
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    • 2022
  • Amid rapid changes in the ICT environment attributed to the 4th Industrial Revolution, the development of information & communication technology, and COVID-19, the existing internet developed without considering security, mobility, manageability, QoS, etc. As a result, the structure of the internet has become complicated, and problems such as security, stability, and reliability vulnerabilities continue to occur. In addition, there is a demand for a new concept of the internet that can provide stability and reliability resulting from digital transformation-geared advanced technologies such as artificial intelligence and IoT. Therefore, in order to suggest a way of implementing the Korean future internet that can strengthen cybersecurity, this study suggests the direction and strategy for promoting the future internet that is suitable for the Korean cyber environment through analyzing important key factors in the implementation of the future internet and evaluating the trend and suitability of domestic & foreign research related to future internet. The importance of key factors in the implementation of the future internet proceeds in the order of security, integrity, availability, stability, and confidentiality. Currently, future internet projects are being studied in various ways around the world. Among numerous projects, Bright Internet most adequately satisfies the key elements of future internet implementation and was evaluated as the most suitable technology for Korea's cyber environment. Technical issues as well as strategic and legal issues must be considered in order to promote the Bright Internet as the frontrunner Korean future internet. As for technical issues, it is necessary to adopt SAVA IPv6-NID in selecting the Bright Internet as the standard of Korean future internet and integrated data management at the data center level, and then establish a cooperative system between different countries. As for strategic issues, a secure management system and establishment of institution are needed. Lastly, in the case of legal issues, the requirement of GDPR, which includes compliance with domestic laws such as Korea's revised Data 3 Act, must be fulfilled.

A Study on Evaluation Method for Structural Suitability of Constructed Wetlands in Dam Reservoirs as an Ecological Water Purification System (생태적 수질정화시설로서 댐 저수지 인공습지의 구조 적정성 평가방안)

  • Bahn, Gwon-Soo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.50 no.2
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    • pp.23-40
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    • 2022
  • Many constructed wetlands have been installed in dam reservoirs nationwide for ecological purification of watershed pollutants, but aging and reduced efficiency are becoming issues. To improve the management of constructed wetlands, an objective evaluation of structural suitability is required. This study evaluated 39 constructed wetlands of 15 dams. First, through fogus group interview(FGI), survey analysis, and analytic hierarchy process(AHP), eight evaluation items in the physical and vegetative aspects were selected and the evaluation criteria applied with weights were prepared. Second, as a result of the structural suitability evaluation, the average score of the overall constructed wetlands was 80.8, with 10 sites rated as 'good grade(91~100)', 22 sites rated as 'normal grade(71~90)' and 7 sites rated as 'poor grade(70 or less)'. The average score of physical structure evaluation was 52.6, with 14 sites rated as 'good', 21 sites as 'normal' and 4 sites as 'poor'. The suitability of location was good level in most constructed wetlands, but the water supply system, depth of water, ratio of length-to-width, and slope of flow channel were evaluated as 'normal' or less in constructed wetlands of 50% or more. Therefore, it was found that overall improvement was necessary for stable flow supply and flow improvement in the constructed wetland. The average score of vegetative structure evaluation was 28.2, and about 84% of them were identified as 'normal' or lower. As a result of analyzing the Spearman's correlation coefficient between the physical structure evaluation score and the vegetation structure evaluation score, there was a significant correlation(r = 0.728, p < 0.001), and it was found that each evaluation factor also influences each other. As a result of the case review of 6 constructed wetlands, the appropriateness of the evaluation results was confirmed, and it was found that the location, flow rate supply, and type of wetland had a great influence on the efficiency and operation of the wetland. Through this study, it will be possible to derive structural weaknesses of constructed wetlands in dam reservoirs as a nature-based solution, to prepare types and practical alternatives for improved management of each constructed wetland in the future, and to contribute to enhancing various environmental functions.

Evaluating the Capping Effects of Dredged Materials on the Contaminated Sediment for Remediation and Restoration of the West Sea-Byeong Dumping Site (서해병 폐기물 배출해역 오염퇴적물의 정화·복원을 위한 준설토 피복 효과 평가)

  • Kang, Dong Won;Lee, Kwang Sup;Kim, Young Ryun;Choi, Ki-young;Kim, Chang-joon
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.2
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    • pp.212-223
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    • 2022
  • For the remediation and restoration of contaminated sediment at the West Sea-Byeong dumping site, dredged materials was dumped in 2013, 2014, 2016, and 2017. The physicochemical properties and benthic fauna in surface sediments of the capping area (5 stations) and natural recovery area (2 stations) were analyzed annually from 2014 to 2020 to evaluate the capping effect of the dredged materials. The natural recovery area had a finer sediment with a mean particle size of 5.91-7.64 Φ, while the sediment in the capping area consisted of coarse-grained particles with a mean particle size of 1.47-3.01 Φ owing to the capping effect of dredged materials. Considering that the contents of organic matters (COD, TOC, and TN) and heavy metals in the capping area are approximately 50 % lower (p<0.05) than that in the natural recovery area, it is judged that there is a capping effect of dredged materials. As a result of analyzing macrobenthic assemblages, the number of species and ecological indices of the capping area were significantly lower than that of the natural recovery area (p<0.05). The number of species and ecological indices at the capping area were increased for the first four years after the capping in 2013 and 2014 and then tended to decrease thereafter. It is presumed that opportunistic species, which have rapid growth and short lifetime, appeared dominantly during the initial phase of capping, and the additory capping in 2016 and 2017 caused re-disturbance in the habitat environment. In the natural recovery and capping areas, Azti's Marine Biotic Index (AMBI) was evaluated as a fine healthy status because it maintained the level of 2nd grades (Good), whereas Benthic Pollution Index (BPI) remained at the 1st and 2nd grade. Therefore, capping of dredged materials for remediation of contaminated sediment in the dumping site has the effect of reducing the pollution level. However, in terms of the benthic ecosystem, it is recommended that the recovery trend should be monitored long-term. Additionally, it is necessary to introduce an adaptive management strategy when expanding the project to remediate the contaminated sediment at the dumping area in the future.

Initial Evaluation using Geochemical Data to infer Tectonic Setting of Mt. Baekdu/Changbaishan Volcano (백두산 화산의 지체구조 추론을 위한 지구화학적 데이터를 이용한 기초 평가)

  • Yun, Sung-Hyo;Chang, Cheolwoo;Pan, Bo
    • Journal of the Korean earth science society
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    • v.43 no.1
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    • pp.128-139
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    • 2022
  • This study aimed to investigate the tectonic setting of the volcanic edifice at Mt. Baekdu by analyzing petrochemical characteristics of Holocene felsic volcanic rocks distributed in the Baekdusan stratovolcano edifice and summit of the Cheonji caldera rim, as well as Pleistocene mafic rocks of the Gaema lava plateau and Changbaishan shield volcano edifice. During the early eruption phases, mafic eruption materials, with composition ranging from alkali basalt to trachybasalt, or from subalkaline (tholeiitic) basalt to basaltic andesite formed the Gaema lava plateau and Changbaishan shield volcanic edifice, whereas the Baekdusan stratovolcano edifice and Holocene tephra deposits near the summit of the Cheonji caldera comprises trachytic and rhyolitic compositions. Analysis results revealed bimodal compositions with a lack of 54-62 SiO2, between the felsic and mafic volcanic rocks. This suggested that magmatic processes occurred at the locations of extensional tectonic settings in the crust. Mafic volcanic rocks were plotted in the field of within-plate volcanic zones or between within-plate alkaline and tholeiite zones on the tectonic discrimination diagram, and it was in good agreement with the results of the TAS diagram. Felsic volcanic rocks were plotted in the field of within-plate granite tectonic settings on discrimination diagrams of granitic rocks. None of the results were plotted in the field of arc islands or continental margin arcs. The primitive mantle-normalized spider diagram did not show negative (-) anomalies of Nb and Ti, which are distinctive characteristics of subduction-related volcanic rocks, but exhibited similar patterns of ocean island basalt. Trace element compositions showed no evidence of, magmatic processes related to subduction zones, indicating that the magmatic processes forming the Baekdusan volcanic field occurred in an intraplate environment. The distribution of shallow earthquakes in this region supports the results. The volcanic rocks of the Baekdusan volcanic field are interpreted as the result of intraplate volcanism originating from the upwelling of mantle material during the Cenozoic era.

A Study on Cultural Planning Based on the Characteristics of Domestic Cultural Archetypes: Focusing on the Jeju Folktale 'Seolmundae Halmang and Obaek General' (국내 문화원형 특징을 기반으로 한 문화 기획 연구: 제주 설화 '설문대 할망과 오백장군'을 중심으로)

  • Lee, Ji-Hun
    • Journal of Korea Entertainment Industry Association
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    • v.15 no.7
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    • pp.259-269
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    • 2021
  • This study examines the legend of 'Grandmother Seolmundae and Five Hundred Generals', the cultural archetypes of Jeju, and analyzes the characteristics of its contents. After analyzing the feasibility of using the analyzed cultural prototype as cultural contents such as games and animations, based on this analysis, we tried to suggest a cultural planning approach based on the cultural prototype to the cultural agency. Therefore, the implications of this study are as follows. First, among the cultural prototypes in Korea, 'Grandmother Seolmundae and Five Hundred Generals', which represent the legends of Jeju Island, are being organized centered on many historical researchers and Chaerokga, but there is no precise establishment of the exact timing of the legend and how it arose. Therefore, when planning and developing content based on the cultural prototype, it is most important for cultural agencies to develop a story after researching historical evidence and opinions of local residents to identify a consistent point of view. Second, although the contents of the cultural archetype are arranged slightly differently by historians and recorders, the main contents and characteristics of the story are found to have a similar form. Therefore, cultural agencies should focus on finding the point of view and characteristics of a story, even if a story is written differently by different people when doing a cultural prototype. Third, when planning a game based on the cultural prototype, the main elements such as the elements to be expressed in the game and the fun elements should be found and presented. In particular, because fun and rules are the most important parts of games, if this part cannot be derived from the story of the cultural archetype or cannot be made, it is difficult to transform the cultural archetype into a game. Therefore, it can be seen that it is important for cultural agencies to set their game plan intentions in consideration of story expression and fun, even if it is the core or non-core of the entire story of the cultural archetype. Lastly, although the cultural prototype 'Grandmother Seolmundae and Five Hundred Generals' was presented as animation content, it is important to develop it considering the story, characters, media, and audience. Therefore, cultural agencies should be able to derive the elements such as stories, representative and auxiliary characters, and viewers that can be adapted from the cultural prototype as much as possible. It will be an important part of raising.