• The Korean Journal of Pesticide Science
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• v.19 no.3
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• pp.323-328
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• 2015

Control efficacy was investigated with fungicides as 3 copper compound, 3 antibiotic fungicides and one fungicide containing to quinolone against the growth of Ralstonia solanacearum on NA medium and the disease occurrence on pepper seedlings. Among 7 fungicides, oxytetracycline was shown the highest activity against a growth of the pathogen in the agar diffusion method, but validamycin showed no activity against the pathogen. With $1000{\mu}g\;mL^{-1}$ of each copper fungicide as copper hydroxide, copper oxychloride+ dithianone and copper sulfate, 2.2, 1.3 and 1.5 mm in size of clear zone only could be found, respectively. In pepper seedling test, oxytetracycline showed a perfect activity in all treatments 7 days after inoculation. However, its activity was decreased from $500{\mu}g\;mL^{-1}$ of treatment over the time. Copper fungicides showed the control efficacy lower than antibiotic fungicides except for validamycin. Based on the results, it was suggested that it would be better to use antibiotic fungicides than copper fungicides to control pepper bacterial wilt in the fields.

• The Korean Journal of Pesticide Science
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• v.17 no.3
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• pp.155-161
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• 2013

The residue patterns of clothianidin and thiacloprid, insecticides registered in the ginseng, were investigated to predict pre-harvest residues limits (PHRL). Pesticides were treated under Korea GAP (Good Agricultural Practices) with the recommended dose (single dose) and twice of recommended dose (double dose). Samples were collected 11 times over 42 days (each 0, 2, 5, 8, 12, 16, 20, 24, 28, 33, 42 days after treatment). Residues of clothinidin and thiacloprid were analyzed by UPLC/TQD. Biological half-life of clothinidin in single dose and double dose were 14.6 days and 10.2 days and that of thiacloprid were also 9.7 days and 11.2 days, respectively. The PHRL of ginseng on 10 days before harvest was 0.3 mg/kg in clothianidin and 0.18 mg/kg in thiacloprid.

• The Korean Journal of Pesticide Science
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• v.17 no.3
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• pp.193-199
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• 2013

Burkholderia pyrrocinia CAB08106-4 has an anti-bacterial effect on Garlic White Rot caused by Sclereotium cepivorum and Sclereotium sp.. It is an environmentally friendly microbial product that prevents and controls a variety of phytopathogens involving Garlic White Rot caused by Sclereotium cepivorum and Sclereotium sp.. The aim of this study was to assess and to compare the pathogenicity of Burkholderia pyrrocinia CAB08106-4 by single exposure of rats through several routes such as oral, intranasal and intravenous. For the acute toxicity / pathogenicity study, the animals were sacrificed on days 3, 7, 14 and 21, and macroscopically observed their organs to examine the numbers of internally-retained pesticidal microbes. Clinical examinations were performed daily during administration period, and body weight gain was evaluated. In the study, no clinical sign, weight gain and mortality were observed in relation to the administration of test article. The significant changes of internal/external microbes by test article were not detected. The pathological findings in relation to the administration of the test article in the necropsy were not observed. It could be concluded that the microorganism was not toxic or pathogenic in rats via oral, intranasal and intravenous route.

• Journal of agricultural medicine and community health
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• v.22 no.1
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• pp.35-41
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• 1997

The purpose of this study is to analyse both actual conditions and health problems of farmers in using peshcrde and to develop protective methods. This study research was carried out by means of questionnaires with members of 100 families randomly selected from 279 families residing within the managing territory of primary health post. The results are as follows: Based on 10 kinds of safety instructions, it was shown that 76% complied with more than 6 isntractrons, and less than 4 instructions were 24%, of which 25% carefully read their handling instructions, 58% did sometimes, and 17% never read such instructions. Not complying with those safety instructions, the most frequent experience was physical symptoms, of which headache accounted for 80.9%, dizziness for 73.5%, dermatitis for 64.7%, and vomiting for 41.2%. Their experiences of pesticide poisoning indicated 68.0%, of which the aged level accounted for 88.9% which was relatively high. A spraying time of peshcide was less than 2 hours accounted for 64.0%, and more than 2 hours for 36%. It was also shown that the number of farmers, who experienced poisoning after spraying such chemicals, accounted for 77.8%. The hate of poisoning experiences were 92.0% in group not wearing a protective equipment, were 62.5% in group wearing a complete set of protective equipment.

• The Korean Journal of Pesticide Science
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• v.14 no.4
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• pp.446-455
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• 2010

The characteristics of parasporal inclusion body from Bacillus thuringiensis subsp. kurstaki KB099 isolate which is high bioactive to the tobacco cutworm, Spodoptera litura, were examined. Parasporal inclusion of B. thuringiensis subsp. kurstaki KB099 isolate showed only 1 band at 130 kDa compared with B. thuringiensis subsp. kurstaki HD-l isolate producing 2 protein bands at 130 kDa and 60 kDa from by SDS-PAGE analysis without any enzyme treatment. Also, we confirmed that gut extract of sensitive S. litura KB099 isolate had digested only 60 kDa ${\delta}$-endotoxin protein. When the digestive enzyme of sensitive insect responsible for parasporal inclusion from KB099 and HD-l isolate was treated to each of them, protein band 60 KDa of KB099 was maintained up to 12 hours but all bands of HD-l were disappeared within 6 hours. In KB099 isolate, 6 genes (Cry1Aa, Cry1Ab, Cry1Ac, Cry1C, Cry1D and Cry1I) were identified by PCR analysis. Also, $Cry^-$ mutant of KB099 isolate was investigated by phase- contrast microscope, SDS-PAGE and PCR.

• The Korean Journal of Pesticide Science
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• v.11 no.4
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• pp.320-330
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• 2007

Mitochondrial 16S DNA sequences of Bemisia tabaci which were collected on rose greenhouse of Iwol and Jinchen in Chungbuk and red pepper field of Miryang, Gyeongnam, were analyzed. The mtCOI PCR product of B. tabaci collected on red pepper field of Miryang were digested with EcoT14I (Sty I) into two fragments 555bp and 311bp, while the PCR product of B. tabaci collected on rose greenhouse of Iwol were digested with Sty I into two fragments of 560bp and 306bp. As a result, B. tabaci collected on red pepper reveal biotype Q and those on rose greenhouse was biotype B. These was difference between two biotypes in insecticide susceptibility, and the biotype B was more susceptible than biotype Q. As a result of foliar systemic test, root-uptake systemic test and residual effect, the biotype B was more susceptible. In case of inhibition effect on enzyme activities of fenitrothion (organophosphorous) and fenothiocarb (carbamate), those of biotype Q was higher than those of biotype B. These results indicate that biotype Q was more resistant than biotype B against 12 insecticides.

• The Korean Journal of Pesticide Science
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• v.12 no.4
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• pp.363-367
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• 2008

Abamectin 1.8% EC and emamectin benzoate 2.15% were evaluated the simultaneous control effect of both insect pests on black pine (Pinus thunbergii) against Thecodiplosis japonensis and Matsucoccus thunbergianae. Abamectin 1.8% EC was highly effective against T. japonensis and M thunbergianae, however, emamectin benzoate was highly effective against only M. thunbergianae when abamectin and emamectin benzoate were applied through trunk injection against T. japonensis and M thunbergianae at the rate of 1 ml per cm in diameter of breast height. The rate of gall formation of T. japonensis was 0% at the applied year, but 85% at the following year. However, the rate of gall formation was over 90% at the treatment of emamectin benzoate showing no difference compared with control. Abamectin 1.8% EC and emamectin benzoate 2.15% showed high control efficacy representing 89.1% and 91.4% against M. thunbergiana, respectively.

• The Korean Journal of Pesticide Science
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• v.18 no.1
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• pp.21-25
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• 2014

Bukholderia pyrrocinia CAB08106-4 has an anti-fungal effect on Garlic White Rot caused by Sclereotium cepivorum and Sclereotium sp. It is environmentally friendly microbial product that prevents and controls a variety of phytopathogens including Garlic White Rot caused by Sclereotium cepivorum and Sclereotium sp. The aim of this study was to assess the environmental toxicity using Cyprinus carpio and Daphnia magna. Bukholderia pyrrocinia CAB08106-4 ($1.0{\times}10^9cfu/mL$) was adminatrated to Cyprinus carpio and Daphnia magna according to the toxicity test guideline for peciticide. $LC_{50}$ of Bukholderia pyrrocinia CAB08106-4 is over $6.67{\times}10^4cfu/mL$ in Cyprinus carpio and Daphnia magna and no adverse effect was observed. Based on these results, we concluded that Bukholderia pyrrocinia CAB08106-4 has no toxiciy for Cyprinus carpio and Daphnia magna.

• The Korean Journal of Pesticide Science
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• v.4 no.4
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• pp.87-92
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• 2000

Recently, a new developed petroleiun spray oil(PSO; D-C Tron $Plus^{(R)}$) has been used to control key pests in integrated pest management (IPM) system of citrus orchards in Australia. The efficacy and phytotoxicity of the PSO against the citrus red mite (Panonychus citri) were compared with conventional pesticides (e.g.Tebufenpyrad, Bifenazate and Sun spray oil) in field condition in Jeju. And under PSO mixing spray with some fungicides, the occurrence of phytotoxic symptoms on citrus leaf was investigated. All concentrations of PSO spraying (0.25%, 0.33%, 0.5% and 1.0%) were significantly suppressed the citrus red mite to similar levels of other conventional pesticides. And the spraying of PSO levels ${\geq}0.5%$ was induced not only occurrence of some oil-sucked symptoms on leaf, but also increase of the dropping leaf and fruit rates. As results from mixing PSO spraying test with other fungicides, little burning on new flush shoot was founded only in PSO 1% plus Fluazinam treatment. From the results of this study, with the consideration of pesticidal efficacy and phytotoxicity, 0.25% and 0.33% PSO spraying level will be recommended for the control of citrus red mite during summer season in Jeju.

• The Korean Journal of Pesticide Science
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• v.12 no.3
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• pp.236-242
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• 2008

Laboratory and greenhouse experiments were conducted to evaluate the effect of root zone application of some systemic insecticides for control of the brown planthopper (BPH), Nilaparvata lugens, as one of the management option to minimize the adverse effects and maximize the efficacy of insecticide application. Five systemic insecticides, namely, carbofuran, carbosulfan, diazinon, ethoprophos and imidacloprid, as granular formulation were placed in the root zone and measured the mortality, fecundity, and nymphal survivorship of the planthopper. Diazinon and ethoprophos did not show the significant mortality of the BPH. When the BPH were inoculated at the day of carbofuran treatment, adult mortality was shown almost 100% at seven days after treatment and BPH nymphs were remained very few on rice at 25 days after treatment. When carbofuran were placed 10 days before the BPH inoculation, it showed almost 100% adult mortality after one day, and no nymphs were found until 25 day after inoculation. Efficacy of carbofuran on BPH when applied in 10 day-old rice was higher than in 30 day-old rice. These results indicated that the root zone application of carbofuran can control N. lugens effectively with less adverse effect to the natural enemies inhabited on rice plants.