• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.22-30
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• 1999

This experiment was carried out to determine the forage yield and mineral contents of silage corn(Zea mays L.), and N balance, soil chemical characteristics and nitrate-N level in infiltration water by different types and N rates of liquid manure applied. Seven treatments consisting of chemical fertilizer $200kg\;N;ha^{-1}$, liquid cattle manure 200, 320 and $440kg\;N;ha^{-1}$, liquid swine manure 200, 320 and $440kg\;N;ha^{-1}$ were arranged in a randomized complete block design with three replications. The results obtained at National Livestock Research Institute, RDA, in Suweon from May 1997 to Aug. 1997 are summarized as follows : There were no significant differences in percentage of dry matter among the treatments, but significant dry matter yield differences were found (P<0.05), and also the mean dry matter yield of liquid swine manure plot was higher than that of liquid cattle manure plot. Potassium, calcium and magnesium contents of silage corn increased with increasing liquid manure application rates (P<0.05). Nitrate-N content of silage corn in the liquid cattle manure treatments was not influenced by liquid manure application rates, but that in the liquid swine manure treatments increased with increasing the N rates of liquid manure application. pH and contents of exchangeable canons of the soil after experiment were raised by increasing the amount of liquid manure application. Available $P_2O_5$ content in topsoil(0-10cm) was highest as $340mg\;kg^{-1}$ at the plot of liquid swine manure $440kg\;N;ha^{-1}$. Concentration of nitrate-N in infiltration water increased at the plot of liquid swine manure $440kg\;N;ha^{-1}$. Amount of nitrogen balance increased with increasing liquid manure application rates. Based on the results of this experiment, it is suggested that the mean dry matter yield of liquid swine manure plot was higher than that of liquid cattle manure plot, and the amount of nitrogen balance increased with increasing liquid manure application.

• Korean Journal of Poultry Science
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• v.45 no.3
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• pp.229-236
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• 2018

The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (${\Delta}K=7.96$). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.33 no.2
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• pp.103-114
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• 2015

UNESCO and IUCN established the term of 'Sacred Natural Sites' (areas of land or water having special spiritual significance to peoples and communities) for conservation of biological and cultural diversity. Dangsan forest, a traditional village forests of rural Korea is a representative 'Sacred Natural Site' with a history of more than several hundred years of Dangsan ritual. The Chungmak village, Gyeokpo-ri, Buan-gun is a small seashore village. It is an important place that has the largest ancient maritime ritual sites in Korea. Buan-gun have been tried to register the 'Chungmak-dong Ritual Site' for the World Heritage List. However, the fact that this 'Chungmak-dong Ritual Site'(5~6 century, Baekje of the Three-kingdom period) was located in the Dangsan forest, surrounding the shrine, is not much understood. In this study, the landscape characteristics and culture of the sacred Dangsan forest at Gyeokpo-ri, including Gyeokpo-ri, Dae-ri, Naesosa temple Seokpo-ri, Buan-gun and Dongho-ri, Gochang-gun were investigated. And, the potential of registering for World Heritage, 'Protected Area of Sacred Natural Sites' by linking the four site's Dangsan forests has been investigated. The sacred Dangsan forests in the neighborhood of Gyeokpo-ri have kept their landscape characteristics and retained Dangsan ritual. As a result of SWOT analysis for sacred natural sites, WT(weakness-threat) strategy has chosen as priority strategy. The reason is that there is few management scheme. The Dangsan forests at the neighborhood of Gyeokpo-ri need to be recognized by people in Korea, for their valuable landscape characteristics. The places should be managed and protected to remain as a sacred natural sites in order to be prepared for a World Heritage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.2
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• pp.106-111
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• 2018

This study was conducted to investigate the relationship between changes of rumen microflora and bloat in Jersey cow. Jersey cows (control age: 42 months, control weight: 558kg; treatment age: 29 months, treatment weight 507kg) were fed on the basis of dairy feeding management at dairy science division in National Institute of Animal Science. The change of microbial population in rumen was analyzed by using next generation sequencing (NGS) technologies due to metabolic disease. The diversity of Ruminococcus bromii, Bifidobacterium pseudolongum, Bifidobacterium merycicum and Butyrivibrio fibrisolvens known as major starch fermenting bacteria was increased more than 36-fold in bloated Jersey, while cellulolytic bacteria community such as Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens was increased more than 12-fold in non-bloated Jersey. The proportion of bacteroidetes and firmicutes was 33.4% and 39.6% in non-bloated Jersey's rumen, while bacteroidetes and firmicutes were 24.9% and 55.1% in bloated Jersey's. In conclusion, the change of rumen microbial community, in particular the increase in starch fermenting bacteria, might have an effect to occur the bloat in Jersey cow.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.819-826
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• 2009

An in vitro study was conducted to investigate the effect of malate or fumarate on fermentation characteristics, and production of conjugated linoleic acid (CLA) and methane ($CH_4$) by rumen microbes when incubated with linolenic acid (${\alpha}-C_{18:3}$). Sixty milligrams of ${\alpha}-C_{18:3}$ alone (LNA), or ${\alpha}-C_{18:3}$ with 24 mM malic acid (M-LNA) or ${\alpha}-C_{18:3}$ with 24 mM fumaric acid (F-LNA) were added to the 150 ml culture solution consisting of 75 ml strained rumen fluid and 75ml McDougall's artificial saliva. Culture solution for incubation was also made without malate, fumarate and ${\alpha}-C_{18:3}$ (Control). Two grams of feed consisting of 70% concentrate and 30% ground alfalfa (DM basis) were also added to the culture solution of each treatment. In vitro incubation was made anaerobically in a shaking incubator up to 12 h at $39^{\circ}C$. Supplementation of malate (M-LNA) or fumarate (F-LNA) increased pH at 6 h (p<0.01) and 12 h (p<0.001) incubation times compared to control and linolenic acid (LNA) treatments. Both malate and fumarate did not influence the ammonia-N concentration. Concentration of total VFA in culture solution was higher for M-LNA and F-LNA supplementation than for control and LNA treatments from 6 h (p<0.040) to 12 h (p<0.027) incubation times, but was not different between malate and fumarate for all incubation times. Molar proportion of $C_3$ was increased by F-LNA and M-LNA supplementation from 6 h (p<0.0001) to 12 h (p<0.004) incubation times compared to control and LNA treatments. No differences in $C_{3}$ proportion, however, were observed between M-LNA and F-LNA treatments. Accumulated total gas production for 12h incubation was increased (p<0.0002) by M-LNA or F-LNA compared to control or LNA treatment. Accumulated $CH_4$ production for 12 h incubation, however, was greatly reduced (p<0.0002) by supplementing malate or fumarate compared to the control, and its production from M-LNA or F-LNA treatment was smaller than that from LNA treatment. Methane production from LNA, M-LNA or F-LNA treatment was steadily lower (p<0.01 - p<0.001) from 3 h incubation time than that from the control, and was also lower for M-LNA or F-LNA treatment at incubation times of 6 h (p<0.01) and 9 h (p<0.001) than for LNA treatment. Methane production from LNA, however, was reduced (p<0.01 - p<0.001) from 3 h to 9 h incubation times compared to the control. Both malate and fumarate increased concentration of trans11-$C_{18:1}$ from 3 h to 12 h incubation (p<0.01), cis9,trans11-CLA up to 6 h incubation (p<0.01 - p<0.01), trans10,cis12-CLA at 3 h (p<0.05) and 12 h (p<0.01), and total CLA for all incubation times (p<0.05) compared to corresponding values for the ${\alpha}-C_{18:3}$ supplemented treatment (LNA). In conclusion, malate and fumarate rechanneled the metabolic $H_2 pathway to production of propionate and CLA, and depressed the process of biohydrogenation and methane generation. Linolenic acid alone would also be one of the optimistic alternatives to suppress the $CH_4$ generation.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.298-305
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• 2014

BACKGROUND: The utilization of green manures as alternatives to reduce the use of chemical fertilizers is considered a good agricultural practice. Effect of incorporation of green manure to soil on change of inorganic nitrogen (N) is well literatured. However, there have been few studies on examining entire dynamic of N including inorganic N and N gases in soil incorporated with green manure. The objective of this study was to examine the changes of inorganic N and N gases with single and mixture applications of hairy vetch and barley in the soil. METHODS AND RESULTS: Hairy vetch(H) and barley (B) were applied at the mixture ratio of B:H=0:0, B:H=100:0, B:H=0:100, and B:H=50:50 in soil. The soil-green manure mixtures were incubated in the dark at $25^{\circ}C$ for 17 weeks under aerobic conditions. Cumulative emission of $NH_3$ and $N_2O$ from soils amended with mixture of barley and hairy vetch(B:H=50:50) were less than those from amended with mono hairy vetch(B:H=0:100). Incorporation of single hairy vetch or mixture of barley and hair vetch application could significantly increased concentration of plant available N ($NH_4{^+}$) in early stage of plant growth and plant available N ($NO_3{^-}$) in later stage. However, high concentration of $NO_3{^-}$ in soil could cause adverse environmental impact through $NO_3{^-}$ leaching from soil. CONCLUSION: Conclusively, it might be a good soil management practice to incorporate mixture of barely and hairy vetch in the view point of increase in plant available N concentration and decrease in N losses through volatilization, denitrification, and leaching.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.4
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• pp.295-300
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• 2008

This experiment was conducted to compare forage production and feed value of winter forage crops at the experimental field of Livestock Division, National Institute of Animal Science from 2003 to 2004 in Jeju. The experiment was arranged in slit plot design with three replications. Main plots consisted of two cutting times, one time cutting and two times cutting. Sub plots consisted of 5 different seed combinations, T1 (Italian ryegrass), T2 (Oat), T3 (Italian ryegrass 25+Oat 75%), T4 (Italian ryegrass 50+Oat 50%), T5 (Italian ryegrass 75+Oat 25%). One time cutting showed the highest dry matter (DM) yield by 18,680 kg/ha. Tow times cutting showed the highest crude protein (CP) content by 11.97%. The all treatments have narrow range of total digestible nutrient (TDN) from 60.6% to 70.4%.

• Korean Journal of Ecology and Environment
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• v.45 no.3
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• pp.263-270
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• 2012

The objectives of the study were to determine fish fauna and compositions during 2010~2011 in Walpyung Conservation Park along with analysis of fish community structures and trophic and tolerance guilds. Total number of species and individuals sampled were 31 and 2667, respectively and dominant species was Zacco platypus (46.6%) and subdominant species was Acheilognathus lanceolatus (8.7%), which were composed of >50% of the total. Total number of Korean endemic species including Microphysogobio yaluensis was 10 and the proportion of the individuals was 8.7%. Also, natural monument No. 454 of Iksookimia choii, which is endangered species and legal protected species, was sampled and the total number of individuals was only three. According to the analysis of fish community structures, species richness index in the mid-stream reach ($M_r$) was 3.145, which is higher than any other stream reaches ($U_r$ and $D_r$). In contrast, the richness index was 2.180 in the up-stream reach ($U_r$), which is minimum among the sampling sites. Species diversity index was 1.785 and 1.975, respectively in the headwater ($U_r$) and mid-stream reach ($M_r$) and the low values in the down-stream reach ($D_r$, 1.660) were due to the influences of pointsource (i.e., road construction) and non-point sources (sporadic agricultural spots). According to analysis of tolerance guilds, the proportion of tolerant species (TS), based on the number of individuals, was composed of 64.2% and sensitive species (SS) was only 3.3%, indicating a predominance of tolerant fishes in the compositions. The proportion of omnivore species (OS), however, was composed of 64.0% and insectivore species (IS) was 29.3%, indicating a predominance of omnivore fishes in the stream. The high proportions of tolerant and omnivore species in this stream indicates that the water quality and physical habitat environments were degradated in this system. For these reasons, especially natural monument and endemic fish species in this region should be protected from the massive constructions and required to provide efficient stream management strategies.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.155-163
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• 1999

Nutrient balance during rice cultivation in the paddy of a local area under the environmental protection for drinking water supply was investigated. To compare nutrient balance in the paddy soil applied with different types of fertilization, 7 treatments were selected as followings : Recommended level of chemical fertilizers(R), Conventional fertilization(CF), Fresh cow manure(FCM), Cow manure compost(CMC), Straw compost+reduced chemical fertilizer(SCF), Fresh straw+recommended level of fertilizers(FSC), and no fertilization as control(C). Here, FCM, CMC and SCF were applied at the same level of total nitrogen as recommended in R. Rice yield was the highest in the recommendation(R) and fresh cow manure (FCM) treatments with $6,730kg\;ha^{-1}$(index 100), and followed by SCF (index 98), FSC (index 98), CMC(index 94), and CF(index 94). But statistically significant difference was not recognized among treatments except the control. Nitrogen infiltration loss was high in the simple chemical fertilizer treatments with $63kg\;ha^{-1}$ in CF and $58kg\;ha^{-1}$ in R during rice cultivation, respectively. Nitrogen infiltration loss was decreased below half level of chemical fertilizer treatments with cow manure treatments ($23kg\;ha^{-1}$ in FCM and $27kg\;ha^{-1}$ in CMC) and with reducing chemical fertilizer treatment by adding straw compost ($25kg\;ha^{-1}$). Phosphate was not leached during rice cultivation in paddy soil of a fluvial deposit type, in which oxidation horizon was developed broadly under around 15 cm depth of surface soil. Phosphate balance (A-B) was closed to 0 in all treatments except cow manure treatment (CMC), in which it was $+30kg\;ha^{-1}$ and show the possibility of over accumulation of phosphate by continuously replicated application of cow manure compost. Potassium balance was negative value in all but straw recycling treatment (FSC). It means that potassium was continuously supplied from soil minerals, uptaken by plants or eluted out of soil. In conclusion, by substituting inorganic fertilizer for organic fertilizer or reducing application rate of chemical fertilizer through mixing organic fertilizer, it would be possible to achieve the same rice yield as in the recommendation treatment and to decrease nutrient leaching below half level in rice paddy soil.

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.3
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• pp.135-152
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• 1975

The following informations are known from physical and chemical characteristics of orchard soils and nutritional diagnosis of orange leaves in Jeju. 1. Most orange orchards are located on terrace and cindercone thus soil moisture and microclimate of an orchard will greatly be affected by its topography. 2. Excessive well drainage, shallow soil depth, high content of gravels, low solid phase ratio and strong wind will give severe problem of soil moisture and wind errosion, thus the exte- nsion of soil depth is necessary for maintain nutrients, water and sufficient root volume. 3. Available soil water was significantly and positively correlated with organic matter content and clay content also contributes to available soil water. Vinyl mulching was greatly helpful for soil water conservation, wind errosion prevention, soil temperature increases during winter. 4. Abundant amphoteric amorphous allophane take a key role to fix phosphorus and also rations and thus it is the major factor to determine fertilizer efficiency. Lime and phosphorus must be applied in deeper soil layer. Release of filed phosphorus must be reevluated for availability. 5. Organic matter such as see weeds will greatly increase fertilizer efficiency and low fertilizer efficiency during spring may be related to available soil water. 6. Nitrogen was in superoptimum and Mg was enough but P and Ca were somewhat deficient according to leaf analysies while K was deficient according to fruit analysis. Phosphorus application increased sugar/acid ratio and potassium decreases rind percentage. 7. Manganese deficiency and toxicity appeared in a few places. Iron and boron were enough. Most places showed tendency of copper excess but some places showed copper deficiency. 8. Soiling after elimination of rock base, application of slow release fertilizer and abundant organic matter, vinyl mulching and drip irrigation will increase fertilizer efficiency greatly and fruit yield drastically.