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http://dx.doi.org/10.5536/KJPS.2018.45.3.229

Genetic Diversity and Relationship of Ogye Population in Korea Using 25 Microsatellite Markers  

Roh, Hee-Jong (Animal Genetic Resources Center, National Institute of Animal Science, RDA)
Kim, Kwan-Woo (Animal Genetic Resources Center, National Institute of Animal Science, RDA)
Lee, Jin-Wook (Animal Genetic Resources Center, National Institute of Animal Science, RDA)
Jeon, Da-Yeon (Animal Genetic Resources Center, National Institute of Animal Science, RDA)
Kim, Seung-Chang (Animal Genetic Resources Center, National Institute of Animal Science, RDA)
Jeon, Ik-Soo (Animal Genetic Resources Center, National Institute of Animal Science, RDA)
Ko, Yeoung-Gyu (Animal Genetic Resources Center, National Institute of Animal Science, RDA)
Lee, Jun-Heon (Division of Animal and Dairy Science, Chungnam National University)
Kim, Sung-Hee (Chungbuk Institute of Livestock and Veterinary Research)
Baek, Jun-Jong (Chungnam Institute of Livestock Experiment Research)
Oh, Dong-Yep (Gyeongbuk Livestock Research Institute)
Han, Jae-Yong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Lee, Seung-Sook (Jisan Farm)
Cho, Chang-Yeon (Animal Genetic Resources Center, National Institute of Animal Science, RDA)
Publication Information
Korean Journal of Poultry Science / v.45, no.3, 2018 , pp. 229-236 More about this Journal
Abstract
The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (${\Delta}K=7.96$). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea.
Keywords
chicken; Ogye; genetic diversity; polymorpism; microsatellite;
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