• Parasites, Hosts and Diseases
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• 제34권4호
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• pp.233-238
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• 1996

이 연구에서는 경기도 양평군 및 광주군에 사는 임산부 899명을 대상으로 IgG-ELISA와 간접 latex 응집반응검사를 시행하여 톡소포자충에 대한 항체가를 측정하였다. IgG-ELISA에서는 0.25 이상을 양성기준으로 하였을 때 음성대조군 218명 중 4명이 양성(1.8%)인 반면 임산부에서는 39 명이 양성으로 검출되어 4.3%의 양성율을 보였다. 간접 latex 응집반응검사는 수의과학연구소에서 만든 킷트(LAT)를 사용하였는데 1:64 희석배수 이상을 양성으로 하였을 때 음성대조군은 모두 음성반응을 보였고 임산부에서는 7명(0.8%)이 양성을 보였다. 임산부중에서 1.8 이상의 반응을 보인 80명을 대상으로 일본제품인 Toxotest-MF를 적용시키고 1:32 이상을 양성의 기준으로 하였을 때 임산부 8명에서 양성반응을 보였다. LAT와 Toxotest-MT의 두 반응간의 일치율은 0.94(${\kappa}-index$ = 0.632. p < 0.01)로 높은 일치율(fair to good agreement)을 보였으므로 LAT는 톡소포자충증의 예비진단에 이용될 수 있을 것으로 생각된다.

• 한국콘텐츠학회논문지
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• 제14권3호
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• pp.346-351
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• 2014

안전한 수혈을 위한 정확한 혈액형 검사는 필수적이다. 최근에는 혈액은행 검사 분야에 검사의 오류를 줄이고 검사의 효율을 높이기 위해 자동화 장비가 도입되어 사용되고 있으나 중소병원에서 이용하기에는 매우 고가로 도입하기가 어렵다. 이에 본 연구에서는 혈액형 검사결과를 저장 및 재확인 할 수 있고, 이미지 프로세싱에 의해 결과가 판독되는 원주응집법을 이용한 혈액형 검사 판독기를 개발하였다. ABO 및 RhD 혈액형 검사가 의뢰된 148개의 검체와 비예기항체 검사가 의뢰된 154개의 검체를 대상으로 판독 결과를 비교한 결과, 양성과 음성의 판독 및 반응강도의 판독이 100%일치하였다. 본 연구에서 개발된 판독기는 추가적인 검증을 거친다면 중소형 병원에서도 쉽게 도입하여 효율적이며, 경제적으로 이용할 수 있을 것이다.

• 한국동물위생학회지
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• 제27권3호
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• pp.281-288
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• 2004

As all other intensively farmed domestic species, most mortality in ostriches is closely to rearing conditions. While ostriches is also highly sensitive to stress, species-specific infectious disease play only a minor role. But investigation of ostrich's disease is not peformed almost in Korea. The study was performed to investigate the titers of antibody for Newcastle disease(ND), Infectious bronchitis(IB), Egg drop syndrome '76(EDS), Avian influenza(AI), salmonellosis, Mycoplasma gallisepticum infection(MG), Mycoplasma synoviae infection(MS), Infectious bursal disease(IBD), Brucellosis, Toxoplasmosis, Japanese encephalitis(JE), Porcine parvovirus infection, Encephalomyocarditis and Porcine reproductive respiratory syndrome (PRRS). The results obtained in the 62 ostrich sera slaughtered in Gyeongbuk province were summarized as follows: The average of antibody positive rates to ND, IB, EDS, AI(H9Nl), JE, Porcine parvovirus infection and Encephalomyocarditis by HI test were $75.8\%,\;100\%,\;0\%,\;0\%,\;51.6\%,\;50\%\;and\;56.5\%$ respectively. The antibody positive rates to salmonellosis, MG, MS by plate agglutination test were $12.9\%,\;25.8\%,\;and\;0\%$ respectively. Antibodies to disease agent such as IBD and AI by agar gel precipitation(AGP) test, Brucellosis by tube agglutination, toxoplasmosis by latex agglutination test and PRRS by IFA were all negative.

• 대한수의학회지
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• 제43권4호
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• pp.677-681
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• 2003

Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.

• Parasites, Hosts and Diseases
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• 제27권3호
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• pp.171-176
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• 1989

강남성모병원의 일반 내원 환자 1,019명, 내원 환자 중 천식으로 진단된 1,030명, 그리고 제주의료원에 내원 한 환자 780면 등 총 2,829명에 대하여 Toxoplasma 항체가를 측정하기 위해 간접 latex 응집반응을 실시하였다. 간접 latex 응집반응의 양성 항체가를 1 : 32로 볼 때, 강남성모병원 일반 내원 환자에서는 19명이 양성으로 1.86%(남자 550명 중 12면으로 2.18%, 여자 469명 중 7명으로 1.49%), 천식 혹은 알레르기 환자에서는 11명이 양성으로 1.07%(남자 470명 중 4명으로 0.85%, 여자 560명 중 7명으로 1.25%)이었으며, 제주의료원 내원환자 에서는 45명으로 5.77%(남자 530명 중 33명으로 6.23%, 여자 250명 중 12명으로 4.80%)이었다. 연령적 분포로는 강남성모병원 일반 환자와 제주의료원 내원 환자의 남자에서 50대에 높은 빈도를 보였고, 전체 여자에서는 일반적으로 30대, 40대 및 60대에서 높은 빈도를 보였다. 양성 항체가를 보인 75명의 항체가별 분포로는 1 32가 32명으로 42.7%, 1 : 64가 21명으로 28.0%, 1 : 128이 8명으로 10.7%, 1 256이 6명으로 8.0%, 1 : 512가 3명으로 4.0%, 그리고 1 : 2,048 혹은 그 이상의 항체가를 보유한 사람은 5명으로 6.7%였다.

• 한국동물위생학회지
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• 제34권2호
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• pp.159-166
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• 2011

This study was carried out to develope enzyme-linked immunosorbent assay (ELISA) for detection of canine brucellosis in dogs experimentally inoculated with Brucella abortus 1119-3 and B. canis RM666. Groups A, B and C of dogs (each group consisting of three dogs) were orally inoculated with approximately $5{\times}10^9$ colony-forming units of B. abortus and B. canis, and with sterile pyrogen-free PBS, respectively. The animals were monitored at regular intervals upto the 12th week post inoculation (PI) by standard tube agglutination test (STAT), plate agglutination test (PAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and ELISA. The induced antibody titers in group A dogs were detected from the first week PI to the eighth week PI in STAT, PAT and RBT using the inactivated whole cells of B. abortus 1119-3 as antigens, while no sera in groups B and C dogs reacted with the antigens. In 2ME-RSAT using whole cells of B. canis M-strain as antigens, the induced antibody titers in group B dogs were observed at the second week PI and persisted for the 12th week PI, while sera of groups A and C dogs did not react with the whole cells. In ELISA using cytoplasmic fractions antigen of B. abortus 1119-3, the mean optical density of antibodies in groups A and B was detected from the first and second weeks PI, respectively, and persisted for 12th week PI, while sera of group C did not cross-react with the fractions antigen. However, in ELISA using the hot saline extracts of B. canis M- as an antigen, the induced antibody titers in only group B dogs were detected from second week PI and persisted for until the end of this study. These results indicate that the ELISA using B. abortus 1119-3 cytoplasmic fractions as antigens can be a good candidate for detection of brucellosis by B. abortus as well as B. canis in dogs.

• Reproductive and Developmental Biology
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• 제37권4호
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• pp.213-217
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• 2013

The objective of this study was to investigate the effect of bacterial contamination on elapsed time after preservation on boar semen. Known numbers of Escherichia coli (E. coli) were inoculated to freshly ejaculated semen and sperm parameters such as viability, motility, agglutination, acrosome integrity and hypo-osmotic swelling test were performed during 7 days of liquid preservation. Semen samples were prepared using antibiotic free BTS extender and 4 different levels of E. coli were treated to semen with following concentrations; 3,000, 5,000, 7,000, 10,000 CFU/ml of sperms. Semen samples were preserved at $17^{\circ}C$ for 7 days in semen storage until analyzed. Aliquots were subjected to measure the sperm viability, motility and agglutination using computer assisted sperm analysis (CASA) system, acrosome integrity was performed using chlortetracycline (CTC) staining method and hypo-osmotic swelling test was performed using hypotonic solution from day 1 (day of semen collection) to 7. Detrimental effects on sperm motility and viability were observed 3 days after preservation at the level of 5,000 CFU/ml (p<0.05). Percentage of sperm abnormality was higher (p<0.05) in over 5,000 CFU/ml groups. Sperm agglutination rate was also significantly higher (p<0.05) in groups of 5,000 and 7,000 CFU/ml. The rate of acrosome reacted sperm was higher as preservation time goes in all the samples but the pattern was clearly higher among E. coli contaminated groups (p<0.05). The sperm membrane integrity in terms of hypo-osmotic test, E. coli affects little compared to other sperm parameters. The deleterious effects observed due to the bacterial contamination in semen suggest that importance of hygiene protocol to minimize the bacterial contamination during semen collection and processing.

• 한국어병학회지
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• 제17권1호
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• pp.11-20
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• 2004

Edwardsiella tarda로 면역한 닭의 난황항체 (IgY) 정제 방법을 비교하였다. Anti-E. tarda IgY의 정제는 PEG법, chloroform-PEG법, ammonium sulfate법과 정제 kit를 사용한 4가지 다른 방법으로 실시하였다. 정제된 IgY는 64 kDa의 heavy chain과 27 kDa의 light chain을 나타내었다. E. tarda로 면역된 IgY는 면역되지 않은 대조 IgY 보다 높은 ELISA가와 응집항체가를 나타내었으며, 정제된 IgY는 western blotting에서 anti-E. tarda 토끼혈청과 유사한 E.tarda 단백질을 인식하였다. PEG법과 ammonium sulfate법에 의해 정제된 IgY는 응집항체가가 1:512, chloroform-PEG법과 정제 kit에 의해 정제된 IgY는 1:128을 나타내었으며, PEG법이 IgY를 정제하기 위한 가장 빠른 방법이었다. 이 연구의 결과로 PEG법이 IgY의 생물학적 활성을 유지함과 더불어 신속하고 효과적인 정제방법임을 알 수 있었다.

• 한국동물위생학회지
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• 제30권2호
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• pp.243-249
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• 2007

The tube agglutination test has been used for bovine brucellosis diagnosis in Korea since middle 1950s. The reported high specificity was its value in eradication program. However, the reading of reaction mostly depends on personal experience, thus here we report a way to improve accuracy and uniformity of reading. The tube agglutination was conducted according to the protocol provided by Korean Ministry of Agriculture and Forestry. The intensity of reaction was measured by spectrophotometer. The relationship between turbidity and percentage clearing was generally in direct proportion and linear. The correspondent percent transmittance at 75, 50, and 25% clearing were 91, 82, and 73, respectively. Then, the degree of percentage of clearing at given international unit was measured. With about 1.5 unit of serum, the maximum percentage clearing was observed. The international unit showing 25, 50, and 75 percentage clearing were 0.61, 0.83 and 1.35, respectively. Based on the information obtained using international standard serum, the calculation of international unit of test serum was available. According to the protocol for bovine brucellosis diagnosis which provided by Korean Ministry of Agriculture and Forestry, the available range of detectable international unit was between 15 and 538. And the corresponding international units for suspicious case ranged between 42 and 127. Of the 35 sera from B abortus infected cattle, about half of them had more than 538 international units. Collectively, the reading of turbidity using spectrophotometer and application to international unit improved accuracy and uniformity of reading.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1975년도 추계학술발표회
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• pp.183.2-183
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• 1975

Ehrlich crcinoma ascites cell을 강하게 응집하는 물질을 Streptomyces sp.에서 얻었으며 이 물질의 성질을 조사하였던바 본 물질은 고분자의 단백총성 물질로 추측되며 용역하게 변성되어 불변성으로 되고 또 이 불변성 물질이 적시 agglutination activity를 가진다는 것이 특징이라고 할 수 있다.(중략)