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Isolation and purification of chicken egg yolk immunoglobulin against Edwardsiella tarda  

Kim, Yeong-Dae (여수대학교)
O, Myeong-Ju (여수대학교)
Jeong, Tae-Seong (경상대학교)
Jeong, Seong-Ju (여수대학교)
Publication Information
Journal of fish pathology / v.17, no.1, 2004 , pp. 11-20 More about this Journal
Abstract
The present study compared purification methods of hen egg yolk immunoglobulin (IgY) from the hen immunized with Edwardsiella tarda. The purification of anti-E. tarda IgY was performed by four different methods, polyethylene glycol (PEG), chloroform polyethylene glycol (Chloroform-PEG), ammonium sulfate and purification kit. Purified IgY had heavy chain of 64 kDa and light chain of 27 kDa size. IgY purified from the hen immunized with E. tarda showed higher ELISA values and agglutination titers than those with IgY purified from the non-immunized hen as a negative control. In addition, purified IgY recognized similar E. tarda proteins to those with anti-E. tarda rabbit serum by western blotting. Purified IgY had an agglutination titer of 1:512 by PEG method and ammonium sulfate method, and 1:128 by chloroform-PEG method and purification kit. Moreover, PEG method was the most rapid method among the four different IgY purification methods. These results indicate that PEG method is effective purification method maintaining biological activity of the IgY.
Keywords
Edwardsiella tarda, IgY, purification method, recognized antigen, agglutination
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1 Jensenius, J.C., Andersen, I., Hau, J., Crone, M. and Koch, C. : Egg: conveniently packaged antibodies, methods for purification of yolk IgG. J. Immunol. Methods, 46 : 63-68, 1981   DOI   ScienceOn
2 Laemmli, U.K. : Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227 : 680-685, 1970   DOI   ScienceOn
3 O'Farrelly, C., Branton, D. and Wanke, C.A. : Oral ingestion of egg yolk immunoglobulin from hens immunized with an enterotoxigenic Escherichia coli strain prevents diarrhea in rabbits challenged with the same strain. Infect. Immun., 60 : 2593-2597, 1992
4 Wakabayashi, H. and Egusa, S. : Seasonal changes of bacterial infections among pond-cultured eels (Anguilla japonica). Fish Pathol., 8 : 91-97, 1973a   DOI
5 Wakabayashi, H. and Egusa, S. : Edwardsiella tarda (Paracolobactrum Anguillimortiferum) associated with pond-cultured eel disease. Nippon Suisan Gakkaishi, 39 : 931-936, 1973b   DOI
6 Yasunaga, N., Ogawa, S. and Hatai, K. : Characteristics of the fish pathogen Edwardsiella isolated from several species of cultured marine fishes. Bull. Nagasaki Prefect. Ins. Fish., 8 : 57-65, 1982. (In Japanese)
7 Yokoyama, H., Peralta, R.C., Diaz, R., Sendo, S., Ikemori Y. and Kodama, Y. : Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets. Infect. Immun., 60 : 998-1007, 1992
8 Hatta, H., Tsuda, K., Akachi, S., Kim, M., Yamamoto, T. and Ebina, T. : Oral passive immunization effect of anti-human rotavirus IgY and its behavior against proteolytic enzyme. Biosci., Biotechnol., Biochem., 57 : 1077-1081, 1993   DOI   ScienceOn
9 Hoshina, T. : Studies on red disease of eels. J. Tokyo Univ. Fish., Spec. Rep., 6 : 1-104, 1962. (In Japanese)
10 Imberechts, H., Deprez, P., Van Driessche, E. and Pohl, P. : Chicken egg yolk antibodies against F18ab fimbriae of Escherichia coli inhibit shedding of F18 positive E.coli by experimentally infected pigs. Vet. microbiol., 54 : 329-41, 1997   DOI
11 Polson, A. : Isolation of IgY from the yolks of eggs by a chloroform polyethylene glycol procedure. Immunol. Invest., 19 : 253-258, 1990   DOI
12 Mamnur Rashid, M., Mekuchi, T., Nakai, T. and Muroga, K. : A serological study on Edwardsiella tarda strains isolated from diseased Japanese flounder (Paralichthys olivaceus). Fish Pathol., 29 : 227, 1994
13 Austin, B. and Austin D.A. : Enterobacteriaceae representatives. In "Bacterial fish pathogens: Disease in farmed and wild fish", 2nded., Ellis Horwood Ltd., Chichester, pp. 188-226, 1993
14 Hatta, H., Kim, M. and Yamamoto, T. : A novel isolation method for hen egg yolk antibody, 'IgY'. Agri. Biol. Chem., 54 : 2531-2535, 1990   DOI   ScienceOn
15 Kusuda, R. and Salati, F. : Major bacterial diseases affecting mariculture in Japan. Ann. Rev. Fish Dis., 3 : 69-85, 1993   DOI   ScienceOn
16 Lee, S.B., Yoshinori M. and Roselynn, M.W. : Effects of hen egg yolk immunoglobulin in passive protection of rainbow trout against Yersinia ruckeri. J. Agri. Food Chem., 48 : 110-115, 2000   DOI   ScienceOn
17 Akita, E.M. and Nakai, S. : Immunoglobulins from egg yolk: isolation and purification. J. Food Sci., 57 : 629-633, 1992   DOI
18 Gutierrez, M.A., Miyazaki, T., Hatta, H. and Kim, M. : Protective properties of egg yolk IgY containing anti-Edwardsiella tarda antibody against paracolo disease in the Japanese eel, Anguilla japonica Temminck & Schlegel. J. Fish Dis., 16 : 113-122, 1993   DOI   ScienceOn
19 Larsson, A., Barlöw, R.M., Lindahl, T.L. and Forsberg, P.O. : Chicken IgG: utilizing the evolutionary advantage. Poultry Science, 72 : 1807-1812, 1993   DOI
20 Nakatsugawa, T. : Edwardsiella tarda isolated from cultured young flounder. Fish Pathol., 18 : 99-101, 1983   DOI
21 Kanai, K., Tawaki, S. and Uchida, Y. : An ecological study of Edwardsiella tarda in flounder farm. Fish Pathol., 23 : 41-47. 1988   DOI
22 Minagawa, T., Nakai, T. and Muroga, K. : Edwardsiella tarda in eel culture environment. Fish Pathol., 17 : 243-250, 1983   DOI
23 Deignan, T., Kelly, J., Alwan, A. and O'farrelly, C. : Comparative Analysis of methods of purification of egg yolk immunoglobulin. Food Agri. Immunol., 12 : 77-85, 2000   DOI   ScienceOn
24 Hoblet, K.H., Kohler, E.M., Saif, L.J., Theil, K.W. and Ingalls, W.L. : Study of porcine postweaning diarrhea involving K88(-) hemolytic Escherichia coli. Am. J. Vet. Res., 47 : 1910-1912, 1986
25 Rose, M.E., Orlans, E. and Buttress, N. : Immunoglobulin classes in hen's egg: their segregation in yolk and white. Eur. J. Immunol., 4 : 521-523, 1974   DOI   ScienceOn
26 Shin, J.H., Yang M.R., Nam, S.W., Kim, J.T., Myung, N.H., Bang, W.G. and Roe, I.H. : Use of egg yolk-derived immunoglobulin as an alternative to antibiotic treatment for control of Helicobacter pylori infection. Clin. Diagn. Lab. Immunol., 9 : 1061-1066, 2002
27 Aoki, T., Arai, T. and Egusa, S. : Detection of R plasmids in naturally occurring fish pathogenic bacteria, Edwardsiella tarda. Microbiol. Immunol., 21 : 77-83, 1977   DOI
28 Polson, A., Von Wechmar, M.B. and Van Regenmortel, M.V.H. : Isolation of viral IgY antibodies from yolks of immunized hens. Immunol. Invest., 9 : 475-493, 1980   DOI
29 Kodama, H., Murai, T., Nakanishi, Y., Yamamoto, F., Mikami, T. and Izawa, H. : Bacterial infection which produces high mortality in cultured Japanese flounder (Paralichthys olivaceus) in Hokkaido. Jpn. J. Vet. Res., 35 : 227-234, 1987
30 Park, S.I., Wakabayashi, H. and Watanabe, Y. : Serotype and virulence of Edwardsiella tarda isolated from eel and their environment. Fish Pathol., 18 : 85-89, 1983   DOI
31 Yolken, R.H., Leister, Wee S.B., Miskuff, R. and Vonderiecht, S. : Antibodies to rotaviruses in chickens eggs: a potential source of antiviral immunoglobulins suitable for human consumption. Pediatrics, 81 : 291-295, 1988