• Food Science of Animal Resources
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• v.37 no.5
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• pp.646-653
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• 2017

Alcalase hydrolysis of liquid egg white was used to produce 5-hydroxytryptophan (HTP) under various conditions and investigate the sleep-potentiating activity of liquid egg white hydrolysate (LEH) on pentobarbital-induced sleep. Alcalase hydrolysis yielded the highest content of 5-HTP ($13.50{\mu}g/mL$), while neutrase hydrolysis showed the lowest 5-HTP content ($5.23{\mu}g/mL$). The liquid egg white to water ratio (1:1) was optimal for the production of 5-HTP with high amino-nitrogen (A-N) content and degree of hydrolysis. The 5-HTP, amino-nitrogen, and degree of hydrolysis increased until 24 h of hydrolysis and slightly increased thereafter during hydrolysis with 2% and 5% enzyme addition. 5-HTP administration at doses of 6 and 9 mg/kg significantly increased sleep duration and decreased sleep latency time compared to that in the control (p<0.05). LEH (150 mg/mouse), which was equivalent to 5-HTP at 6 mg/kg, significantly decreased sleep latency time and increased sleep duration time compared to that in the control (p<0.05). Oral administration of LEH showed sleep-potentiating effects because of 5-HTP. The sleep-potentiating activity of LEH may have occurred through 5-HTP in our pentobarbital-induced sleep model. LEH may be a valuable alternative to sleep enhancement and may be used as a sleep-potentiating agent.

• Food Science of Animal Resources
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• v.37 no.2
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• pp.242-253
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• 2017

This study was performed to evaluate antioxidant activity of tomato powder extracted by various concentrations of ethanol (0, 25, 50, 75, 100%) and to evaluate the physicochemical properties and antioxidant activities of pork patties with ethanol extracted tomato (EET) powder. No differences in the contents of total of individual phenolic compounds including gallic acid and catechin, were observed among the treatments (p>0.05). Among the various concentrations, 50% and 75% EET powder showed the highest free radical scavenging and iron chelating activities (p<0.05). Lipid peroxidation was retarded in linoleic acid emulsion with the addition of 50% and 75% EET powder (0.1%). Based on the model study, five pork patties were actually manufactured; control patty, reference patty with 0.01% of butylated hydroxytoluene, patty with 1% of water extracted tomato (WET), and patties with 0.5 and 1.0% of EET. Addition of 1% WET and EET decreased pH value, and increased redness values of pork patties, as compared to the control (p<0.05). Pork patties with WET (1.0%) and EET (0.5% and 1.0%) had lower 2-thiobarbituric acid reactive substances values compared with control patties after 7 d of storage (p<0.05). Pork patties containing EET powder showed lower total bacterial and Enterobacteriaceae counts than control patties (p<0.05). In conclusion, WET and EET (50%) could be used as a natural antioxidant and antimicrobial agent in meat products.

• Journal of Adhesion and Interface
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• v.17 no.4
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• pp.149-154
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• 2016

In this study, natural polymer-based virus neutralizing agent was developed in an attempt to replace the conventional sterilization method for mammalian cell culture. A catechol conjugated heparin was synthesized by using EDC chemistry, and it show unique binding ability to virus which has heparin affinity (adenovirus, adeno-associated virus). To evaluate neutralization ability of catechol conjugated heparin, adeno-associated virus was used for test model, instead of using a pathogenic virus. The catechol conjugated heparin exhibited resistance to high concentration of salt and complete inactivation of adeno-associated virus. The result suggests that the catechol conjugated heparin, which is biocompatible and efficiency, may replace conventional sterilization method for mammalian cell culture.

• Journal of Microbiology and Biotechnology
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• v.30 no.3
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• pp.325-332
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• 2020

Methyl linderone (ML), a cyclo-pentenedione, was isolated from the fruit of Lindera erythrocarpa Makino (family Lauraceae). This plant has well-known anti-inflammatory effects; however, the anti-cancer effects of ML have not yet been reported. Thus, in the present study we investigated the effects of ML on the metastasis of human breast cancer cells. We used 12-O-tetradecanoyl phorbol-13-acetate (TPA)-stimulated MCF-7 cells as the cell model to study the effects of ML on invasion and migration. ML was found to reduce the invasion and migration rate of TPA-stimulated MCF-7 cells. Moreover, it inhibited two metastasis-related factors, matrix metalloproteinase-9 (MMP-9) and interleukin-8 (IL-8), at the mRNA and protein expression levels, in TPA-treated MCF-7 cells. The mechanism by which ML exerted these effects was through the inhibition of translocation of activator protein-1 (AP-1) and signal transducer and activator of transcription-3 (STAT3), mediated via phosphorylation of extracellular signal-regulated kinase (ERK). Taken together, our findings indicated that ML attenuated the TPA-stimulated invasion and migration of MCF-7 cells by suppressing the phosphorylation of ERK and its downstream factors, AP-1 and STAT3. Therefore, ML is a potential agent for the treatment of breast cancer metastasis.

• The Journal of the Korea Contents Association
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• v.9 no.12
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• pp.932-939
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• 2009

This paper implements the graphic service template authoring tool based on the service template object model of describing the meta information of the services for early childhood education in the semantic web service environment. Our proposed system provides the robot services by constructing web services automatically and making the appropriate service plans. Moreover, it can create, append, delete, and update the service templates of URC based on STDL, and provide the graphic function on service template resources. In order to provide the user friendly environment in the service template phase, we implement the various editing environment : flow view style, grid view style, and text view style. We also provide the easy editing function by realizing abstract service block based on the robot API. Finally we can offer the intelligent and autonomous service of service agent based on semantic information.

• The KIPS Transactions:PartB
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• v.17B no.1
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• pp.85-92
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• 2010

A shallow semantic parsing system analyzes the relationship that a syntactic constituent of the sentence has with a predicate. It identifies semantic arguments representing agent, patient, instrument, etc. of the predicate. In this study, we propose a two-phase shallow semantic parsing model which consists of the identification phase and the classification phase. We first find the boundary of semantic arguments from partial syntactic parsing results, and then assign appropriate semantic roles to the identified semantic arguments. By taking the sequential two-phase approach, we can alleviate the unbalanced class distribution problem, and select the features appropriate for each task. Experiments show the relative contribution of each phase on the test data.

• YAKHAK HOEJI
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• v.36 no.6
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• pp.518-528
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• 1992

To determine the optimal conformation of sulfonylureas, the correlation between conformation and hypoglycemic activity of the two sulfonylureas of tolbutamide and chlorpropamide as hypoglycemic agent was studied using an empirical potential function (ECEPP/2) and the hydration shell model in the unhydrated and hydrated states. The conformational energy was minimized from several starting conformations with possible torsion angles in each molecule. The conformational entropy change of each conformation was computed using a harmonic approximation. To understand the hydration effect on the conformation of the molecules in aqueous solution, the contribution of water-accessible volume of each group or atom in the lowest-free-energy conformation was calculated and compared each other. From comparison of the computed lowest-free-energy conformations of two sulfonylureas, it could be suggested that the hydration of sulfonylurea moiety is related to increase the hypoglycemic activity. From the calculation results, it was known that the conformational entropy is the major contribution to stabilize the low-free-energy conformations of two sulfonylureas in unhydrated state. Whereas, in hydrated state, the hydration free energy largely contributes to the total free energies of low-free-energy conformations of tolbutamide and conformational entropy contributes to stabilize the low-free-energy conformations of chlorpropamide. The torsion angles from phenyl ring to urea moiety of the low-free-energy conformations of the two sulfonylureas were shown the nearly regular trend. On the basis of these results, the conformation exhibiting the optimal hypoglycemic activity of sulfonylureas and the binding direction to pancreatic receptor site A could be predicted. Also, according to the side chain lengthening of urea moiety, tolbutamide showed various conformational change. Therefore, steric effect may be important factor in the interaction between sulfonylureas and the putative pancreatic receptor.

• Preventive Nutrition and Food Science
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• v.17 no.4
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• pp.245-253
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• 2012

Collagen tripeptide (CTP) is a functional food material with several biological effects such as improving dry skin and wound and bone fracture healing. This study focused on the anti-photoaging effects of CTP on a hairless mouse model. To evaluate the effects of CTP on UVB-induced skin wrinkle formation in vivo, the hairless mice were exposed to UVB radiation with oral administration of CTP for 14 weeks. Compared with the untreated UVB control group, mice treated with CTP showed significantly reduced wrinkle formation, skin thickening, and transepidermal water loss (TEWL). Skin hydration and hydroxyproline were increased in the CTP-treated group. Moreover, oral administration of CTP prevented UVB-induced MMP-3 and -13 activities as well as MMP-2 and -9 expressions. Oral administration of CTP increased skin elasticity and decreased abnormal elastic fiber formation. Erythema was also decreased in the CTP-treated group. Taken together, these results strongly suggest that CTP has potential as an anti-photoaging agent.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.5 no.7
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• pp.1329-1345
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• 2011

After the 9.11 terror attack, lawful Interception (LI) has emerged as an important tool for anti-terrorist activity. Law enforcement agents and administrative government bodies effectively monitor suspicious target users of permanent IP-based network devices by LI in Packet Data Networks (PDNs). However, it is difficult to perform LI in monitoring migrating users from a location to another, who change their IPs due to the proliferation of portable Internet devices enabling 3G IP Multimedia Subsystems (IMS). The existing, manual handover technique in 3G IMS makes it even more difficult to continue the LI activities due to time-lag reissuance of LI authority warrants when the target users move to a new LI jurisdiction via a roaming service. Our proposed model is a seamless LI handover mechanism in 3G IMS to support mobility detection of the target users. The LI warrants are transferred to the new LI agent automatically with the target users when they move to a new LI jurisdiction. Thus, time-lag human intervention of reissuance of the LI warrants is removed and enables the LI authorities to continue monitoring. In the simulation of our proposed mechanism, the quality of lawful interception achieves a mean score of over 97.5% out of the possible 100% maximum score, whereas the quality of the existing mechanism has a mean score of 22.725%.

• Fire Science and Engineering
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• v.29 no.4
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• pp.26-32
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• 2015

Carbon dioxide principally extinguishes fires by smothering, but an acceptable amount of extinguishing agent is needed. To assure the performance of carbon dioxide systems in Korea, computer programs certified by NEMA are being applied in system design. But the design errors can occur because the geometry of a model test facility is not the same as that of the actual fire area. Since the discharge rate tends to vary considerably with the flow pattern in a pipe, an on-site discharge test is necessary to ensure the performance of the system, especially with low pressure carbon dioxide. Technical standards for carbon dioxide systems do not give detailed guidelines for discharge tests at present. Based on comparative analysis of standards and practical tests, this paper suggests a methodology for on-site discharge tests.