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http://dx.doi.org/10.17702/jai.2016.17.4.149

Significant Attenuation of Aden-associate Virus Gene Expression by Catechol-conjugated Heparin Surface Coating  

Do, Minjae (Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST))
Lee, Slgirim (Department of Chemical and Biomolecular Engineering, Yonsei University)
Jang, Jae-Hyung (Department of Chemical and Biomolecular Engineering, Yonsei University)
Lee, Haeshin (Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST))
Publication Information
Journal of Adhesion and Interface / v.17, no.4, 2016 , pp. 149-154 More about this Journal
Abstract
In this study, natural polymer-based virus neutralizing agent was developed in an attempt to replace the conventional sterilization method for mammalian cell culture. A catechol conjugated heparin was synthesized by using EDC chemistry, and it show unique binding ability to virus which has heparin affinity (adenovirus, adeno-associated virus). To evaluate neutralization ability of catechol conjugated heparin, adeno-associated virus was used for test model, instead of using a pathogenic virus. The catechol conjugated heparin exhibited resistance to high concentration of salt and complete inactivation of adeno-associated virus. The result suggests that the catechol conjugated heparin, which is biocompatible and efficiency, may replace conventional sterilization method for mammalian cell culture.
Keywords
neutralization; adeno-associated virus; heparin; catechol;
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