• Title/Summary/Keyword: active sites

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Developmental characterization of embryo size mutant in rice (Oryza sativa L.)

  • Hong, Soon-Kwan
    • Plant Resources
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    • v.5 no.2
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    • pp.141-154
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    • 2002
  • In this experiment, three kinds of mutations(ge, re, and eml )relating to the size of embryos were used to study their generation, genetic mechanism and developmental characteristics, and the interactions between embryo and endosperm were also examined. Giant embryo mutation comprises 7 kinds including the already isolated ge, and ge-2, which share an identical gene site. The SAM and the size of radicule for the ge showed little difference compared to a normal type. The number of embryo cells did not increased as much as it would affect the size of embryo. Therefore, the enlargement of embryo was due to the enlargement of scutellum that originated from the corpulence of each cell. Both F$_1$' s of re ]and odm 49 formed reduce embryos, and other combinations of hybridization showed all wild type of embryo sizes. Accordingly, the odm 49 must have an identical gene site of re 1, while odm 48 and odm 62 have different gene sites. Their shoots and radicules also shrank by the same ratio, however no sign of physical change was noticed. The size of embryo cell showed no change, while the number of cells was the half of that of wild types. The three gene sites of re represent all of them control the size of the entire embryo forming organs. The eml 1 was defined to have temperature sensibilities that the generation of endosperms was active at a high temperature while that was hampered at a low temperature.

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Understanding Information Sharing Among Scientists Through a Professional Online Community: Analyses on Interaction Patterns and Contents

  • Shin, Eun-Ja;Lee, Guiohk;Choi, Heeyoon
    • Journal of Information Science Theory and Practice
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    • v.5 no.4
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    • pp.26-38
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    • 2017
  • Even through many professional organizations increasingly use Q&A sites in their online communities for information sharing, there are few studies which examine what is really going on in the Q&A activities in professional online communities (POC). This study aims to examine the interaction patterns and contents posted in the Q&A site of a POC, KOSEN, a science and technology online community in South Korea, focusing on how actively scientific information and knowledge are shared. The interaction patterns among the participants were identified through social network analysis (SNA) and the contents in the Q&As were examined by content analysis. The results show that the overall network indicated a moderate level of participation and connection and answerers especially tended to be active. Also, there are different interaction patterns depending on academic fields. Relatively few participants were posting leaders who seemed to steer the overall interactions. Furthermore, some content related to manipulation and explanation for experiments, which are in urgent need, seem to be posted in the sites more frequently with more amounts. Combining both SNA and content analysis, this study demonstrated how actively information and knowledge is shared and what types of contents are exchanged. The findings have practical implications for POC managers and practitioners.

Role of Disulfide Bond of Arylsulfate Sulfotransferase in the Catalytic Activity

  • Kwon, Ae-Ran;Choi, Eung-Chil
    • Archives of Pharmacal Research
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    • v.28 no.5
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    • pp.561-565
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    • 2005
  • Bacterial arylsulfate sulfotransferase (ASST) catalyzes the transfer of sulfate group from a phenyl sulfate ester to a phenolic acceptor. The promoter region and the transcripti on start sites of Enterobacter amnigenus astA have been determined by primer extension analysis. Northern blot analysis resolved two mRNA species with lengths of 3.3 and 2.0 kb, which correspond to the distances between the transcriptional initiation sites and the two inverted repeat sequences (IRSs). By length, the 3.3 kb RNA could comprise the three-gene (astA with dsbA and dsbB) operon. ASST has three highly conserved cysteine residues. Reducing and non-reducing SDS-PAGE and activity staining showed that disulfide bond is needed for the activity of the enzyme. To identify the cysteine residues responsible for the disulfide bond formation, a series of Cys to Ser mutants has been constructed and the enzymatic activity was measured. Based on the results, we assumed that the first cysteine (Cys349) might be involved in disulfide bond mainly with the second cysteine (Cys445) and result in active conformation.

An Analysis on the Visual Structure from the Building Area around An-ap Pond (안압지 호안 건물지의 조망 경관구조 분석)

  • 박경자;이관규;양병이
    • Journal of the Korean Institute of Landscape Architecture
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    • v.29 no.2
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    • pp.14-21
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    • 2001
  • This study aims to analyze visual structure by evaluating the view from five building sites around An-ap pond, and attempt to determine which site commands the best view and will provide the most active use. The results of this study can be summarized as follows: According to the questionnaire survey of experts on the relations of dominancy-subordination(´chu-jong´), vacancy-solidness(´heo-sil´), sparsity-density(´so-mil´) based on ancient oriental Yin-Yang theory and analysis of visual structure on angle of elevation, depression, and the landscape-component ratio to be seen through five building sites around the west of An-ap pond, building site three was selected as the building site which has the best landscape. Therefore, it is estimated that building site three played the role of core-building site. According to the result of correlation analysis, the greater the increased in the component ratio of sky, mountain ,the greater the degree of harmony within the landscape. As well, the degree of harmony increased when the landscape component ratio of a distant view was greater than that of a near view. Moreover, it was proved that the relationships of ´chu-jong´, ´heo-sil´, ´so-mil´ are correlative, not independent.

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Surface Tentiometric Studies on the Interaction of Anionic Polyelectrolytes with Cationic Surfactants

  • Park, Joon-Woo;Lee, Jin-Gyu;Lee, Hoo-Sung
    • Bulletin of the Korean Chemical Society
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    • v.10 no.4
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    • pp.339-343
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    • 1989
  • The interaction of cationic surfactants, n-alkyltrimethylammonium bromide ($C_nTAB$; n = 12, 14, 16) with anionic polyelectrolyte, poly(styrenesulfonate) (PSS) has been studied by surface tension measurement. In the absence of added salt, the cationic surfactants bind to PSS quantitatively up to ca. 60% coverage of anionic sites of the polyanion and the complexes were surface inactive. Further binding of the surfactant cations on PSS caused a sharp conformational transition of the surfactant/ PSS complexes to surface active complexes and accompanied precipitation. The binding showed a biphasic behavior in the presence of NaCl and cooperativity of the binding became less as the concentration of NaCl increased. Binding of the cationic surfactants on poly(vinylsulfonate) also showed the biphasic behavior and the cooperativity of the binding was much less even in the absence of NaCl. The binding of surfactant to PSS provided hydrophobic environment to solubilized pyrene and reduced the viscosity of the solution greatly even at surfactant concentrations well below cmc. This study indicated that the surfactant bound to PSS up to $60{\%}$ coverage of PSS sites are present as surfactant aggregates which are wrapped up with PSS chains, and hydrophobic interaction is an important factor in the binding of the surfactants to PSS.

Lattice Oxygen Activation in NiFe (Oxy)hydroxide using Se (셀레늄을 활용한 니켈철 (옥시)수산화물의 격자 산소 활성화)

  • Jo, Seunghwan;Sohn, Jung Inn
    • Korean Journal of Materials Research
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    • v.32 no.8
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    • pp.339-344
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    • 2022
  • The lattice oxygen mechanism (LOM) is considered one of the promising approaches to overcome the sluggish oxygen evolution reaction (OER), bypassing -OOH* coordination with a high energetic barrier. Activated lattice oxygen can participate in the OER as a reactant and enables O*-O* coupling for direct O2 formation. However, such reaction kinetics inevitably include the generation of oxygen vacancies, which leads to structural degradation, and eventually shortens the lifetime of catalysts. Here, we demonstrate that Se incorporation significantly enhances OER performance and the stability of NiFe (oxy)hydroxide (NiFe) which follows the LOM pathway. In Se introduced NiFe (NiFeSe), Se forms not only metal-Se bonding but also Se-oxygen bonding by replacing oxygen sites and metal sites, respectively. As a result, transition metals show reduced valence states while oxygen shows less reduced valence states (O-/O22-) which is a clear evidence of lattice oxygen activation. By virtue of its electronic structure modulation, NiFeSe shows enhanced OER activity and long-term stability with robust active lattice oxygen compared to NiFe.

A Study on the Interpretation of Cultural Heritage Sites for Public Education (대중교육을 위한 문화유적의 해석(Interpretation)에 관한 기본연구)

  • Han, Sang-U
    • KOMUNHWA
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    • no.68
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    • pp.41-57
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    • 2006
  • The purpose of this study is to review educational value of cultural heritage sites first and then, to examine the importance of interpretation and interpretation methods and process briefly to utilize cultural heritage sites as important resources for public education and cultural tourism. The educational value of cultural heritage sites is not limited to provide visitors with various information on historical facts and events related to the cultural heritage site, but to provide the public with a new recognition of the importance of the preservation of cultural heritage sites by understanding the site well. In order to utilize the educational value of the cultural heritage site, interpretation must be emphasized as an important element of cultural heritage sites management and much attention should be given on the development and practice of interpretation programs in each cultural heritage site. In order to develop an interpretation program of the site, a specific target audience must be defined first to find most resonable scope and direction of an interpretation program and then, site specific interpretation themes (or a theme) should be developed from the interpretive topic. According to these defined elements, an interpretation program(s) is established by using various interpretation methods and techniques in order to provide visitors with useful information and knowledge on the site efficiently. Interpretation methods and techniques are various as defined by various ways and most effective methods or techniques are employed as a specific interpretation program is presented most effectively. The evaluation processes in planing stage, during developing stage, and after completion of interpretation program development are important processes to find out any problems of planed interpretation program and to revise or update the interpretation program. Conclusively, the direction of cultural heritage sites management in Korea has focused on the conservation and restoration of cultural heritage sites to preserve the original conditions of cultural heritage sites so far. This direction on current practice on cultural heritage sites management should he changed to more active utilization of the sites and interpretation of the sites is a way to accomplish the this goal.

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Depolymerization of Kraft Lignin over a Ru-Mg-Al-oxide Catalyst (Ru-Mg-Al-oxide 촉매 상에서 크라프트 리그닌의 저분자화 연구)

  • Kim, Han Ung;Limarta, Susan Olivia;Jae, Jungho
    • Clean Technology
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    • v.27 no.2
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    • pp.190-197
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    • 2021
  • Kraft lignin is a by-product of the pulp and paper industry, obtained as a black liquor after the extraction of cellulose from wood through the Kraft pulping process. Right now, kraft lignin is utilized as a low-grade boiler fuel to provide heat and power but can be converted into high-calorific biofuels or high-value chemicals once the efficient catalytic depolymerization process is developed. In this work, the multi-functional catalyst of Ru-Mg-Al-oxide, which contains hydrogenation metals, acid, and base sites for the effective depolymerization of kraft lignin are prepared, and its lignin depolymerization efficiency is evaluated. In order to understand the role of different active sites in the lignin depolymerization, the three different catalysts of MgO, Mg-Al-oxide, and Ru-Mg-Al-oxide were synthesized, and their lignin depolymerization activity was compared in terms of the yield and the average molecular weight of bio-oil, as well as the yield of phenolic monomers contained in the bio-oil. Among the catalysts tested, the Ru-Mg-Al-oxide catalyst exhibited the highest yield of bio-oil and phenolic monomers due to the synergy between active sites. Furthermore, in order to maximize the extent of lignin depolymerization over the Ru-Mg-Al-oxide, the effects of reaction conditions (i.e., temperature, time, and catalyst loading amount) on the lignin depolymerization were investigated. Overall, the highest bio-oil yield of 72% and the 3.5 times higher yield of phenolic monomers than that without a catalyst were successfully achieved at 350 ℃ and 10% catalyst loading after 4 h reaction time.

Constructing a Conceptual Framework for the Development of Cultural Tourism based on 'place image' and 'local systems for cultural activities' ('장소의 상징적 이미지와 문화적 활동의 영역적 체계'에 입각한 문화관광개발의 개념적 모형 정립)

  • Lee, Jeong-Hoon
    • Journal of the Korean association of regional geographers
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    • v.11 no.5
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    • pp.405-425
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    • 2005
  • Transition from mass tourism to post tourism is closely related with developing various types of cultural tourism. This study attempts to construct a conceptual framework of the development of cultural tourism through identifying the existing development stages of cultural tourism sites from birth to full development. This study clarifies that there exists an organic system among tourism, cultural activities and the production of cultural artifacts. It also found that development of cultural tourism sites goes through the following three stages: positioning and building place image, hard branding, and constructing local system. This study analyzes several key elements for respective of stages, which proved helpful in understanding the development mechanism of cultural tourism sites. It also tries to analyze tourism from an integrated, geographical perspective. The analysis gives us an understanding to the relations between tourism and various aspects of regional society and economy, thus contributing to the development of a system that will lead to active interchanges among those factors. Most of the theoretical background of this study: place identity, place image, activity space, local system and network are based on the concepts and ideas of human geography.

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A Study on Nano/micro Pattern Fabrication of Metals by Using Mechanical Machining and Selective Deposition Technique (기계적 가공과 무전해 선택적 증착기술을 이용한 나노/마이크로 금속패턴 제작에 관한 연구)

  • Cho S.H.;Youn S.W.;Kang C.G.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.1507-1510
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    • 2005
  • This study was carried out as a part of the research on the development of a maskless and electroless process for fabricating metal micro/nanostructures by using a nanoindenter and an electroless deposition technique. $2-\mu{m}-deep$ indentation tests on Ni and Cu samples were performed. The elastic recovery of the Ni and Cu was 9.30% and 9.53% of the maximum penetration depth, respectively. The hardness and the elastic modulus were 1.56 GPa and 120 GPa for Ni and 1.49 GPa and 100 GPa for Cu. The effect of single-point diamond machining conditions such as the Berkovich tip orientation (0, 45, and $90^{\circ}$) and the normal load (0.1, 0.3, 0.5, 1, 3, and 5 mN), on both the deformation behavior and the morphology of cutting traces (such as width and depth) was investigated by constant-load scratch tests. The tip orientation had a significant influence on the coefficient of friction, which varied from 0.52-0.66 for Ni and from 0.46-0.61 for Cu. The crisscross-pattern sample showed that the tip orientation strongly affects the surface quality of the machined area during scratching. A selective deposition of Cu at the pit-like defect on a p-type Si(111) surface was also investigated. Preferential deposition of the Cu occurred at the surface defect sites of silicon wafers, indicating that those defect sites act as active sites for the deposition reaction. The shape of the Cu-deposited area was almost the same as that of the residual stress field.

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