• The Journal of the Korea Contents Association
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• v.8 no.7
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• pp.26-34
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• 2008

Due to spread of movies or online games which are applied with computer animation techniques, we can easily see scenes where numerous characters appear. In the case of large-scale crowd animation, if one were to increase reality of the scene, features of system would be lowered, and if one were to increase functioning of system, reality of the scene would be lowered. In realizing large-scale crowd animation with seafloor environment as background, the paper analyzed and applied elements that affect behavioral types of fishes; and by using concept of crowd, the paper enabled each group or object to control their behavioral type; by comparing and contrasting real-time calculation method as calculation method for animation and hybrid calculation method which is mixed calculation method, the paper seeks to find a method that increases functioning of the system while also expresses natural scenes.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.13 no.3
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• pp.185-192
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• 1993

A numerical model has been used for the prediction of wave agitations in a harbor which are induced by the intrusion and transformation of incident waves. Based on linear wave theory a mild-slope equation has been used. A partial absorbing boundary condition has been used on solid boundary. Functional has been derived following Chen and Mei(l974)'s technique based on Hybrid Element Method which uses finite discretisation in the inner region and analytical solution of Helmholtz equation in the outer region. Final simultaneous equation has been solved using the Gaussian Elimination Method. Helmholtz natural period and second peak period of seiche in Donghae Harbor coincide very well with the results from numerical calculation. Computed amplification factors show good agreement, especially when the reflection coefficient on solid boundary is 0.99, with those of measurements.

• Earthquakes and Structures
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• v.20 no.3
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• pp.353-364
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• 2021

Natural hazards like earthquakes, high winds, and tsunami are a threat all the time for multi-story structures. The environmental forces cannot be clogged but the structures can be prevented from these natural hazards by using protective systems. The structural control can be achieved by using protective systems like the passive, active, semi-active, and hybrid protective systems; but the semi-active protective system has gained importance because of its adaptability to the active systems and reliability of the passive systems. Therefore, a semi-active protective system for the earthquake forces has been adopted in this work. Magneto-Rheological (MR) damper is used in the structure as a semi-active protective system; which is connected to the current driver and proposed controller. The Proportional Integral Derivative (PID) controller and reliable PID controller are two proposed controllers, which will actuate the MR damper and the desired force is generated to mitigate the vibration of the structural response subjected to the earthquake. PID controller and reliable PID controller are designed and tuned using Ziegler-Nichols tuning technique along with the MR damper simulated in Simulink toolbox and MATLAB to obtain the reduced vibration in a three-story benchmark structure. The earthquake is considered to be uncertain; where the proposed control algorithm works well during the presence of earthquake; this paper considers robustness to provide satisfactory resilience against this uncertainty. In this work, two different earthquakes are considered like El-Centro and Northridge earthquakes for simulation with different controllers. In this paper performances of the structure with and without two controllers are compared and results are discussed.

• Microbiology and Biotechnology Letters
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• v.40 no.2
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• pp.92-97
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• 2012

Antimicrobial peptides (AMPs) are important components of living organisms acting against Gram-negative and Gram-positive bacterial and fungal pathogens. Cathelicidin human peptides have a variety of biological activities that can be used in clinical applications. AMPs are not produced naturally in large quantities, and chemical synthesis is also economically impractical, especially for long peptides. Therefore, as an alternative, heterologous expression of AMPs by recombinant techniques has been studied as a means to reduce production costs. E. coli is an excellent host for the expression of AMPs, as well as other recombinant proteins, because of the low cost involved and its easy manipulation. However, overexpression of AMPs in E. coli has been shown to cause difficulties resulting from the toxicity of the subsequently produced AMPs. Therefore, fusion expression was theorized to be a solution to this problem. In this study, AMPs were expressed as fused proteins with the glutathione S-transferase (GST) binding protein to protect against the toxicity of AMPs when expressed in E. coli. The LL37, and hybrid gaegurin and LL37 (GGN4(1-16)-LL37(17-32), which we designated as GL32, peptides were expressed as GST-fusion proteins in E. coli and the fusion proteins were then purified by affinity columns. The purified peptides were obtained by removal of GST and were confirmed by western blot analysis. The purified antimicrobial peptides then demonstrated antimicrobial activities against Gram-negative and Gram-positive bacterial strains.

• Structural Engineering and Mechanics
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• v.29 no.1
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• pp.91-111
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• 2008

This paper outlines the development of a computational model in order to analyze the dynamic behaviour of coupled fluid-structure systems such as a) liquid containers, b) a set of parallel or radial plates. In this work a hybrid fluid-solid element is developed, capable of simulating both membrane and bending effects of the plate. The structural mass and stiffness matrices are determined using exact integration of governing equations which are derived using a combination of classical plate theory and a finite element approach. The Bernoulli equation and velocity potential function are used to describe the liquid pressure applied on the solid-fluid element. An impermeability condition assures a permanent contact at the fluid-structure interface. Applications of this model are presented for both parallel and radial plates as well as fluid-filled rectangular reservoir. The effect of physical parameters on the dynamic behaviour of a coupled fluid-structure system is investigated. The results obtained using the presented approach for dynamic characteristics such as natural frequency are in agreement to those calculated using other theories and experiments.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.04a
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• pp.266-271
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• 2009

A modeling method for the modal analysis of a multi-packet blade system having a crack undergoing rotational motion is presented in this paper. Each blade is assumed as a slender cantilever beam. The stiffness coupling effects between blades due to the flexibilities of the disc and the shroud are modeled with discrete springs. Hybrid deformation variables are employed to derive the equations of motion. The flexibility due to crack, which is assumed to be open during the vibration, is calculated basing on a fracture mechanics theory. To obtain more general information, the equations of motion are transformed into dimensionless forms in which dimensionless parameters are identified. The effects of the dimensionless parameters related to the angular speed, the depth and location of a crack on the modal characteristics of the system are investigated with some numerical examples.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.16 no.4
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• pp.97-103
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• 2017

The objective of this study is to develop and commercialize an on-board fuel storage system for CNG vehicles. A type 4 vessel is made of resin-impregnated continuous filament windings on a polyamide (PA6) liner. In particular, this study localized the PA6 liner's fabrication and development. To analyze the filament winding, a specimen test was performed, and the results were verified values obtained using finite element analysis. In this study, the filament winding and fibers were optimized for a 207 bar composite cylinder in a compressed natural gas vehicle.

• Journal of Microbiology
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• v.34 no.4
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• pp.327-333
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• 1996

Yeast, like many other microbes, encounters large variations in ambient pH in their natural environments. Microorganisms capable of growing over a wide pH range require a versatile, efficient pH homeostatic mechanism protecting intracellular processes against extremes of pH. In several organisms, fusions to the bacterial lacZ gene have been extremely useful for the identification of genes expressed at different time during the life cycle or under different growth conditions. In this study, using the lacZ gene screening system, we surveyed a large number of yeast strains with lacZ insertion to identify genes regulated by pH. A yeast genomic library was constructed and inserted with lacZ by a shuttle mutagenesis procedure. The yeast transformants were individually picked up with a toothpick, replica-plated, and grown in alkaline pH medium. Among the 35,000 colonies screened, 10 candidate strains were identified initially by the $\beta$-gal assay. We finally confirmed two yeast strains carrying the genes whose expression are strictly dependent on pH of growth medium. One of the fusions showing a 10-fold induction in expression level in response to alkali pH was selected and further characterized. The pH-regulated gene was cloned by inverse PCR and a partial sequence of the gene was determined. Identification and characterization of the gene is currently under investigation.

• Korean Chemical Engineering Research
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• v.48 no.3
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• pp.350-354
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• 2010

In natural world various inorganic crystals are grown with controlled shape and size in hybrid forms with organics. Such natural processes have been attempted much to mimick artificially. One of the example is calcium carbonate which has been examined a lot in the field of biomineralization. In this study, we utilize well-organized surface of polydiacetylene(PDA) films as the crystal-growing template. We devised a novel mini-arrayer device that transfers PDA films at air/water interfaces of each array well and deposit them to hydrophobized glass substrates. This technical improvement will contribute to facilitate better understandings of biomineralization mechanism.

• Transactions of the Korean hydrogen and new energy society
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• v.31 no.5
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• pp.405-410
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• 2020

In this literature, we are introduce a basic design of multi energy hub based on natural gas governor station. Multi energy hub consists of turbo expender generator, phosphoric acid fuel cell, pressure swing adsorption, H₂ charging station, utilities and etc. We design a hybrid energy hub system that provides energy using these complex energies, and calculates the amount of electricity that can be produced and the amount of hydrogen charged through the process analysis. TEG and phosphoric acid fuel cell produce 2,290 to 2,380 kW and can supply electricity to 500 houses. In addition, By-product H₂ gas is refined to H₂ vehicle fuel. This will help maximize the balance of energy demand and supply and improve national energy efficiency by integrating unused decompression energy power generation technology and various power generation/heat source technologies.