• Journal of Life Science
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• v.14 no.4
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• pp.670-675
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• 2004

We have examined the effects of S-nitroso-N-acetylpenicillamine (SNAP), prostaglandin $E_2$ (PG $E_2$) and dibutric cyclic AMP (dbcAMP) on the methylation of interferon- ${\gamma}$ (IFN- ${\gamma}$ ) gene in human Jurkat T cells. The CpG dinucleotide which is critical for promoter function of IFN- ${\gamma}$ gene was methylated by treatment with SNAP, PG $E_2$ and dbcAMP, respectively. The DNA methylation induced by PG $E_2$ was suppressed by the addition of 2',5'-dideoxyadenosine (DDA), an inhibitor of adenylyl cyclase, but the suppression was not observed in SNAP treated cells. The NO production was not enhanced in PG $E_2$ or dbcAMP treated cells. The methylation induced by PG $E_2$ and dbcAMP was not suppressed by the addition of $N^{G}$-methyl-L-arginine (L-NMMA), NO synthase inhibitor. In conclusion, the inhibition of INF- ${\gamma}$ gene expression by PG $E_2$ was associated with the methylation of INF- ${\gamma}$ gene by elevation of intracellular cAMP in human Jurkat T cells. However, the methylation induced by PG $E_2$ might not be mediated through the NO production.rough the NO production.

• Journal of Food Hygiene and Safety
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• v.25 no.2
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• pp.153-161
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• 2010

The purpose of this study was to examine the appearance of norovirus in the water for food in food service institutions and the influence of physicochemical and microbial factors of norovirus in order to work out basic data to predict the detection of norovirus. Among 82 samples of water for food in food service institutions, norovirus appeared in 7 samples and the rate of appearance was 8.5%. As for the type of norovirus, one samples contained GI type (genotype GI-6) and six samples contained GII type (genotype GII-2, GII-4, GII-12). In the regression model of prediction of norovirus, the rate of appearance was correlated with $NH_3$-N, total solids and the consumption of $KMnO_4$, out of such variables as $NH_3$-N, total solids, the consumption of $KMnO_4$, depth, chloride and total colony counts, and its contribution rate for effectiveness was 78.60%. In order to examine the influential factor of environment upon the detection of norovirus, Pearson's correlation analysis was carried out. The predictable regression formula for appearance rate of norovirus was expressed as -1.818 + 42.677 [$NH_3$-N] + 0.023 [total solids] + 0.762 [consumption of $KMnO_4$] -0.009 [depth] -0.146 [chloride] + 0.007 [total colony counts] (R = 0.904, $R^2$ = 0.818, adjusted $R^2$ = 0.786, p < 0.05). The most influential factors upon the detection of norovirus were $NH_3$-N, total solids and the consumption of $KMnO_4$. In other words, when the measured values of $NH_3$-N, total solids and the consumption of $KMnO_4$ were higher, the possibility of appearance of norovirus increased.

• The Journal of the Petrological Society of Korea
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• v.19 no.1
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• pp.1-18
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• 2010

On the ArcGIS 9.2 program in Gyeongsangbug-Do and Daegu areas, distribution ratios of rock types and geologic ages were obtained from the 1 : 250,000 scaled digital geologic and geomorphic maps. The obtained distribution ratios here will be used the geologic information data for industrialization and development planning of rock resources. The Gyeongsangbug-Do area consists of 86 rock types that can be divided into 10 large groups in geologic age. Their geologic distribution ratios show the decreasing in the order of Cretaceous, Precambrian, Jurassic, Quaternary, Age-unknown and Tertiary, all of which occupy the prevailing ratio of 96.30% in the area. Of which, sixteen rock types are somewhat dominant ones (64.04%). They are of Precambrian Yulri group and granite gneiss of the Yeongnam metamorphic complex and biotite gneiss of the Sobaegsan metamorphic complex, Age-unknown granite, Jurassic granite, Cretaceous Gasongdong and Dogyedong formations of the Yeongyang sub-basin, Nagdong and Chunsan formations and intermediate-basic volcanics of Euiseong sub-basin, Jinju and Jindong formations and andesite-andesitic tuff of Milyang sub-basin, and hornblende granite, and Quaternary alluvium. They show relatively narrow ranges of 2.07-6.53% in geologic distribution in exception of Jurassic granite showing 13.14%. And the rest 70 rock types appear to very narrow range between 0.01 and 1.94 %. On the other hand, twelve rock types are developed in the Daegu area. Their geologic ages appear to be classified into Cretaceous and Quaternary occupying 86.05% and 11.39%, respectively. Seven rock types take possession of 94.04% among the all rocks. The major rock types are Jinju formation of the Sindong group, Chilgog, Haman and Jindong formations of the Hayang group, andesite and andesitic tuff, hornblende granite and Quaternary alluvium. With exception of andesite and andesitic tuff of 37.40%, the types show slightly wide range of 3.25-17.39%, which apparently differ trends from that of Gyeongsangbug-Do area. And the rest of rock types have narrow ranges of 0.22-1.81% in the Daegu area.

• Korean journal of food and cookery science
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• v.32 no.4
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• pp.390-399
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• 2016

Purpose: The objective of this study was to examine the effect of plant scale and cooking conditions on the quality characteristics of sausages during refrigerated storage. Methods: Sausages used in this study were classified into two groups: those submitted to $1^{st}$ cooked treatments and those submitted to $2^{nd}$ cooked treatments. The pH, volatile basic nitrogen (VBN), gas production ratio, and microorganisms were measured in triplicate. Results: The change of quality in the products was assessed every 7 days by measuring pH, VBN levels, total microbes, coliform bacteria, Escherichia coli, and pathogenic bacteria in the products. Pathogenic bacteria such as Staphylococcus aureus, Listeria monocytogenes, Clostridium perfringens, and E. coli were not detected in the sausages with $1^{st}$ cooked treatments. The results showed that the pH of the sausages decline as storage time increased. The pH value of the sausages with $2^{nd}$ cooked treatments changed gradually. VBN levels were generally lower in products with $2^{nd}$ cooked treatments than in those with $1^{st}$ cooked treatments, but they varied with the type of products. On the $35^{th}$ day, the number of total microbes ranged between 6.13-7.12 log CFU/g in products with $1^{st}$ cooked treatments and 3.44-6.92 log CFU/g in products with $2^{nd}$ cooked treatments, showing fewer bacteria in the latter products. Conclusions: $1^{st}$ cooked treatments were effective in microbial control, but $2^{nd}$ cooked treatments could prolong the shelf life of the sausages, indicating a need for differential management of each product.

• Korean Journal of Food Science and Technology
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• v.37 no.6
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• pp.882-888
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• 2005

Procedure for analysis of methylmercury in fish was developed, involving addition of HCl, extraction with toluene, and clean-up using L-cystein solution. Obtained extract is analyzed by gas chromatography with electron capture detector using Ulbon HR-Thermon-Hg column. Detection limit and recovery of the method were 0.005mg/kg (expressed as Hg), 98-107 (103%), respectively. Total mercury and methylmercury concentrations in 175 commercial fish samples ranged from [mean-max (mean), unit: mg/kg]: 0.014-1.200 (0.270) and 0.006-0.901 (0.168) in tuna-fish, 0.020-0.934 (0.323) and 0.012-0.553 (0.149) in martin-fish, 0.082-0.782 (0.391) and 0.040-0.436(0.201) in shark, 0,023-0.031 (0.026) and 0,013-0.018 (0.015) in salmon, 0.098-0.193 (0.133) and 0.031-0.015(0.090) in tilefish, and 0,031-0.214 (0.089) and 0.016-0.093 (0.042) in canned tuna respectively. No sample of analyzed fish exceeded 1.0mg/kg wet wt., limit for methylmercury established by Codex. In all species examined, estimated weekly intake was lower than Provisional Tolerable Weekly Intake recommended by the JECFA (the Joint FAO/WHO Expert Committee on Food Additives).

• The Journal of Korean Society for Radiation Therapy
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• v.26 no.1
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• pp.59-67
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• 2014

Purpose : This study aims to evaluate 3D dosimetric impact for MIP image and each phase image in stereotactic body radiotherapy (SBRT) for lung cancer using volumetric modulated arc therapy (VMAT). Materials and Methods : For each of 5 patients with non-small-cell pulmonary tumors, a respiration-correlated four-dimensional computed tomography (4DCT) study was performed. We obtain ten 3D CT images corresponding to phases of a breathing cycle. Treatment plans were generated using MIP CT image and each phases 3D CT. We performed the dose verification of the TPS with use of the Ion chamber and COMPASS. The dose distribution that were 3D reconstructed using MIP CT image compared with dose distribution on the corresponding phase of the 4D CT data. Results : Gamma evaluation was performed to evaluate the accuracy of dose delivery for MIP CT data and 4D CT data of 5 patients. The average percentage of points passing the gamma criteria of 2 mm/2% about 99%. The average Homogeneity Index difference between MIP and each 3D data of patient dose was 0.03~0.04. The average difference between PTV maximum dose was 3.30 cGy, The average different Spinal Coad dose was 3.30 cGy, The average of difference with $V_{20}$, $V_{10}$, $V_5$ of Lung was -0.04%~2.32%. The average Homogeneity Index difference between MIP and each phase 3d data of all patient was -0.03~0.03. The average PTV maximum dose difference was minimum for 10% phase and maximum for 70% phase. The average Spain cord maximum dose difference was minimum for 0% phase and maximum for 50% phase. The average difference of $V_{20}$, $V_{10}$, $V_5$ of Lung show bo certain trend. Conclusion : There is no tendency of dose difference between MIP with 3D CT data of each phase. But there are appreciable difference for specific phase. It is need to study about patient group which has similar tumor location and breathing motion. Then we compare with dose distribution for each phase 3D image data or MIP image data. we will determine appropriate image data for treatment plan.

• Korean Journal of Food Science and Technology
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• v.34 no.5
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• pp.762-769
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• 2002

As a preliminary test for defining intact yellow croaker pigment, the pigment was analyzed by column chromatography and UV-vis spectrophotometry. All maximum absorbance wavelengths commonly showed three maximum absorbance ranges, similar to those of carotenoid, suggesting that the tested pigment may be carotenoid. We detected total six peak RT values in the chromatogram through PDA-HPLC under gradient mode (behavior A at 10% for initial 2 min and changed to behavior B for 60 min). Most pigments were detected at the peak with 3.27 RT value. Because seven peaks were detected under gradient mode and three under isocratic mode [methanol : methylene chloride (90 : 10, v/v)], gradient mode was determined to be more appropriate for quantitative analysis. By the comparison test of RT values among yellow pigment in croakers and reference pigments, such as zeaxanthine, ${\beta}-cryptoxanthine$, ${\beta}-carotene$, and astaxanthin, only ${\beta}-cryptoxanthine$ was detected in the white croaker, whereas such pigment of yellow croaker having RT value of 31.02 was not detected. Therefore, RT value was found to be applicable for detecting adulterated croaker.

• The Journal of the Petrological Society of Korea
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• v.20 no.1
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• pp.39-59
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• 2011

To get the geologic information data such as rock resources, industrial ground, development planning and so on, distribution ratios of constituent rocks with geologic age and rock type were obtained in Gyeongnam, Ulsan and Busan areas by ArcGIS 9.3 program, digital geologic and geomorphic maps of 1 : 250,000 scale. Geologic ages and rock types in the Gyeongnam area can be divided into 6 and 40, respectively. Their distribution ratios of the geologic ages are decreasing in the order of Cretaceous, Precambrian, Quaternary, Jurassic, Triassic and Tertiary. They show the wide ranges of 1.35-57.36%, and the former makes the most dominant ratio. Major rock types are 24 ones, all of which occupy the ratio of 94.58% and relatively narrow ranges of 1.15-13.64% in the area. Among them, andesite and andesitic tuff shows the more or less dominant ratio, and separately develops in the northeast, mid east and south parts of the area. In the Ulsan area, geologic ages and rock types can be divided into 3 and II, respectively. Their distribution ratios of the geologic ages are decreasing in the order of Cretaceous, Quaternary and Triassic. They show the very wide range of 6.90-79.21%, and the former makes the most prevailing ratio. Major rock types are 9 ones, which totally occupy the ratio of 98.63% and more or less wide ranges of 1.50-39.01% in the area. Among them, Jindong formation shows the most dominant ratio, and widely develops in the inner and eastern part of the area. In the Busan area, geologic ages and rock types can be divided into 3 and 10, respectively. Their distribution ratios of the geologic ages are decreasing in the order of Cretaceous, Quaternary and Tertiary. They show the wide ranges of 6.73-47.02%, and the two former makes the most dominant ratio of 88.03%. Major rock types are 6 ones, all of which occupy the ratio of 93.02% and relatively wide ranges of 4.07-47.02% in the area. Among them, alluvium forms the most dominant ratio, which mostly develops in the lower Nagdong River, West Nagdong River and Suyeong River.

• The Journal of the Petrological Society of Korea
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• v.22 no.2
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• pp.153-177
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• 2013

To get the various data on geological information, distributional ratios and characteristics of constituent rocks with geologic ages and rock types were obtained by ArcGIS 10.1 program, digital geologic and geomorphic maps of 1:250,000 scale in Jeonnam and Gwangju areas. In the Jeonnam area, geologic ages can be largely divided into 7, in which their distribution ratios show decreasing trends in the order of Cretaceous, Precambrian, Jurassic, Quaternary, Age-unknown, Carbonifeorus-Triassic and Triassic, and the former fours make the most prevailing ratios of 94.80%. Rock types in the area can be assorted into 57 ones, in which major 7 ones occupy the dominant ratio of 71.68%. Among them, Kav (acidic volcanics+rhyolite and rhyolitic tuff) show much more distribution ratios than the others. It shows more aspects distributed in north, west, middle, east and south parts, especially in Sinan-Mogpo-Yeongam of west and Haenam of south parts in the area, respectively. On the other hand, geological ages in Gwangju area can be largely divided into 5, in which their distribution ratios show decreasing trends in the order of Jurassic, Quaternary, Cretaceous, Precambrian and Age-unknown, and the former fours occupy almost the whole ratio of 98.95%. Rock types in the area are 12 ones, in which major four ones make up the dominant value of 91.30%. Among them, Jurassic granites of the most dominant value are mostly occupied in the southwest-northeast part of the area. Next dominative Quaternary alluvium is mostly developed along the Yeongsan river, the Hwangryong river and their channel junction. And Yongdu and Donggye plains are well developed around the Yeongsan riverline, and channel junction of the Yeongsan and Hwangryong rivers in the area, respectively.

• The Journal of Korean Society for Radiation Therapy
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• v.16 no.2
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• pp.33-41
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• 2004

Purpose : Film has been the primary tool in coincidence testing between the light field and the radiation field, which constitutes the quality assurance list of a linear accelerator. But there is a great chance of errors being different among the observer when using film. Thus this study set out to use the BIS(Beam Image System) in addition to film in comparing and evaluating coincidence results between the two fields and in searching for the improvement measures. Materials & Methods : Photon beam of 6 and 15MV was exposed to film and the BIS using a linear accelerator. The light and radiation fields were each $50{\times}50,\;100{\times}100,\;and\;200{\times}200mm^2$. The gantry angle was $0^{\circ}$ when using film and $0^{\circ}\;and\;270^{\circ}$ when using the BIS. The devices adopted to test coincidence between the two fields were a ruler and film scanner when using film. With the BIS, the width of the scanned light and radiation fields was measured for errors with setting the X and Y axis. Results : The visual measurements of the observer with film resulted that the radiation field was bigger than the light field and that their maximum error was 1.9mm. The results were the same with the measurements using the film scanner except for the average error, which was less than 1.9mm. On the contrary, the measurements using the BIS showed that the light field was bigger than the radiation field at the gantry angle of $0^{\circ}\;and\;270^{\circ}$. The maximum error was 0.96mm, and the error range was $<{\pm}2mm$ both in the X and Y axis. The average error of ${\Delta}X$, Y was the smallest in the order of the visual film measurements, film scanner measurements, and BIS measurements Conclusion . This requires a careful measurement for accurate quality assurance since errors are much different according to each observer that tests coincidence between visual fields with film. And an observer needs to use another image device or develop a measuring device of his own if it seems necessary for accurate measurements.