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Investigation of Norovirus Occurrence and Influence of Environmental Factors in Food Service Institutions of ChungCheong Area  

Jung, Woo-Young (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Eom, Joon-Ho (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Kim, Byeong-Jo (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Yun, Min-Ho (Bio Environmental Chemistry, Chungnam National University)
Ju, In-Sun (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Kim, Chang-Soo (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Kim, Mi-Ra (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Byun, Jung-A (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Park, You-Gyoung (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Son, Sang-Hyuck (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Lee, Eun-Mi (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Jung, Rae-Seok (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Na, Mi-Ae (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Yuk, Dong-Yeon (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Gang, Ji-Yeon (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Heo, Ok-Sun (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Publication Information
Journal of Food Hygiene and Safety / v.25, no.2, 2010 , pp. 153-161 More about this Journal
Abstract
The purpose of this study was to examine the appearance of norovirus in the water for food in food service institutions and the influence of physicochemical and microbial factors of norovirus in order to work out basic data to predict the detection of norovirus. Among 82 samples of water for food in food service institutions, norovirus appeared in 7 samples and the rate of appearance was 8.5%. As for the type of norovirus, one samples contained GI type (genotype GI-6) and six samples contained GII type (genotype GII-2, GII-4, GII-12). In the regression model of prediction of norovirus, the rate of appearance was correlated with $NH_3$-N, total solids and the consumption of $KMnO_4$, out of such variables as $NH_3$-N, total solids, the consumption of $KMnO_4$, depth, chloride and total colony counts, and its contribution rate for effectiveness was 78.60%. In order to examine the influential factor of environment upon the detection of norovirus, Pearson's correlation analysis was carried out. The predictable regression formula for appearance rate of norovirus was expressed as -1.818 + 42.677 [$NH_3$-N] + 0.023 [total solids] + 0.762 [consumption of $KMnO_4$] -0.009 [depth] -0.146 [chloride] + 0.007 [total colony counts] (R = 0.904, $R^2$ = 0.818, adjusted $R^2$ = 0.786, p < 0.05). The most influential factors upon the detection of norovirus were $NH_3$-N, total solids and the consumption of $KMnO_4$. In other words, when the measured values of $NH_3$-N, total solids and the consumption of $KMnO_4$ were higher, the possibility of appearance of norovirus increased.
Keywords
Norovirus; physicochemical factor; microbial factor; one-step RT-PCR; semi-nested PCR;
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Times Cited By KSCI : 5  (Citation Analysis)
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