• KSBB Journal
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• 제24권5호
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• pp.425-431
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• 2009

본 연구에서는 U-251-MG 세포를 이용하여 증식, 이동, 침윤 및 단백질분해효소의 분비에 미치는 PSA와 HGF의 효과를 확인하였다. 그 결과, PSA siRNA에 의해 U-251-MG 세포의 증식은 약 37% 억제되었으나, HGF 처리 (10 ng/mL)에 의해 증식이 약 1.4배 증가되었다. PSA siRNA에 의한 U-251-MG 세포의 이동은 약 60%가 억제되었으나, HGF 처리에 의해 이동이 약 1.3배 증가되었다. 또한, PSA siRNA에 의해 U-251-MG 세포의 침윤이 약 67% 억제되었으나 HGF 처리에 의해 세포의 침윤이 약 4.3배 증가되었다. PSA siRNA처리에 의해 MMP-2의 분비는 약 25%, MMP-9의 분비는 약 20% 억제되었으며, HGF에 의해 PSA siRNA에 의해 억제된 MMP-2 및 MMP-9의 분비가 약 2.8배 및 3.5배 증가되었다. HGF 처리에 의해 플라스민의 분비량은 약 14배 증가되었고, PSA를 억제한 U-251-MG 세포에 HGF를 처리한 결과, 플라스민의 분비가 약 1.6배 증가하였다. 또한, PSA siRNA에 의해 MMP-2의 발현은 유의할만한 변화가 없었으나, MMP-9의 발현은 약 85% 억제되었다. PSA를 억제시킨 U-251-MG 세포에 HGF를 처리한 결과, MMP-2의 발현은 약 5.7배, MMP-9의 발현은 약 6.3배 증가되었다. 한편, MMPs의 광범위 억제제인 BB-94 처리에 의해 U-251-MG 세포의 증식, 이동 및 침윤이 유의할만하게 억제 된 것은 MMP-2 및 MMP-9이 U-251-MG 세포의 증식, 이동 및 침윤에 관여할 것임을 시사해준다.

• 생약학회지
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• 제30권2호
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• pp.99-104
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• 1999

During the screening for inhibitors of nitric oxide production in LPS-activated macrophage, RAW 264.7 cells. Five coumarins were isolated from chloroform extract of the root of Peucedanum japonicum. They were identified as praeruptorin A (1), xanthotoxin (2), psoralen (3), isopimpinellin (4), bergapten (5) on the basis of spectroscopic methods. The $IC_{50}$ values for nitrite production by activated macrophages were about $1.5\;{\mu}g/ml$ (1), $0.3\;{\mu}g/ml$ (2), $1.0\;{\mu}g/ml$ (3), $25\;{\mu}g/ml$ (4), $25\;{\mu}g/ml$ (5), respectively. However, the inducible nitric oxide synthase (iNOS) was not inhibited by treatment with these compounds. Their inhibitory effect on nitric oxide production was resulted from the supperssion of iNOS expression.

• Animal Systematics, Evolution and Diversity
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• 제27권1호
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• pp.25-33
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• 2011

Three morphospecies of the genus Uronychia, i.e. U. setigera Calkins, 1902, U. binucleata Young, 1922, and U. multicirrus Song, 1997, were collected from the coastal waters of Gumjin-ri on the East Sea and the public waterfront of Incheon on the Yellow Sea in Korea, respectively. These species are described based on live observation, protargol impregnation, silver nitrate impregnation, and their morphometrics. Diagnostic keys for these species are also provided. In addition, their small subunit ribosomal DNA sequences were compared with previously known sequences of Uronychia species. Diagnostics of three Uronychia species are as follows: U. setigera: $50-80\;{\mu}m$ long in vivo, oval-shaped, 2 macronuclear nodules (Ma), 1 spur on the left margin, 11 adoral membranelles (AM) 1, 4 AM2, 1 buccal cirrus (BC), 4 frontal cirri (FC), 3 left marginal cirri (LMC), 2 ventral cirri (VC), 5 transverse cirri (TC), 3 caudal cirri (CC), 6 dorsal kineties (DK), and approximately 23 cilia in the leftmost kinety. U. binucleata: $70-110\;{\mu}m$ long in vivo, oval to slightly rectangular shaped, 2 Ma, 1 micronucleus (Mi), 2 spurs on the posterior region, 11 AM1, 4 AM2, 1 BC, 4 FC, 3 LMC, 2 VC, 5 TC, 3 CC, 6 DK, and approximately 37 cilia in the leftmost kinety. U. multicirrus: $140-200\;{\mu}m$ long in vivo, oval to slightly rectangular shaped, ca. 7 Ma, 1 Mi, 11 AM1, 4 AM2, 1 BC, 4 FC, 3 LMC, approximately 8 VC, 5 TC, 3 CC, and 6 DK. This study presents the first record of this genus in Korea.

• Spatial Information Research
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• 제17권4호
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• pp.405-416
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• 2009

도시의 패러다임이 변화하고 이에 따라 u-City의 수요가 급증하고 있다. 우리나라에서만 2009년 5월을 기준으로 54개 지역에서 u-City가 구축되고 있다. 이렇게 급증하는 u-City의 성패를 좌우하는 중요 요소 중 하나가 바로 u-City에서 제공하고자 하는 u-서비스라고 할 수 있겠다. 현재의 u-서비스는 센서네트워크를 기반으로 하는 도시 관리 위주의 모니터링 서비스가 대부분이다. 유비쿼터스의 본질적 의미인 '언제 어디서나'의 u-서비스를 구현하기 위해서는 공간정보의 활용이 필수적이다. 공간정보와 센서정보의 결합은 공간인지(spatial awareness) 를 가능케 하여, 모니터링서비스에서 보다 나아가 공간분석이 가능한 서비스로의 발전을 도모할 수 있다. 따라서 본 연구에서는 1) u-서비스에서 spatial awareness는 어떤 의미 인지를 명확히 하고, 2) spatial awareness를 가능케 하기 위해 공간정보, 센서정보, 기타 정보들이 어떻게 융합 (Spatial Embedding) 되어야 하는지 논하고, 3) U-GIS를 기반으로 한 u-서비스 시나리오 4가지를 제안하고, 4) 현재의 기술개발 현황에 대해 분석해 보고자 한다.

• The Korean Journal of Physiology and Pharmacology
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• 제18권5호
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• pp.377-381
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• 2014

Propofol is a widely used anesthetic. Many studies have shown that propofol has direct effects on blood vessels, but the precise mechanism is not fully understood. Secondary intrapulmonary artery rings from male rats were prepared and mounted in a Multi Myograph System. The following constrictors were used to induce contractions in isolated artery rings: high $K^+$ solution (60 mmol/L); U46619 solution (100 nmol/L); 5-hydroxytryptamine (5-HT; $3{\mu}mol/L$); or phenylephrine (Phe; $1{\mu}mol/L$). The relaxation effects of propofol were tested on high $K^+$ or U46619 precontracted rings. Propofol also was added to induce relaxation of rings preconstricted by U46619 after pretreatment with the nitric oxide synthase inhibitor $N^G$-nitro-L-arginine methyl ester (L-NAME). The effects of propofol on $Ca^{2+}$ influx via the L-type $Ca^{2+}$ channels were evaluated by examining contraction-dependent responses to $CaCl_2$ in the absence or presence of propofol (10 to $300{\mu}mol/L$). High $K^+$ solution and U46619 induced remarkable contractions of the rings, whereas contractions induced by 5-HT and Phe were weak. Propofol induced dose-dependent relaxation of artery rings precontracted by the high $K^+$ solution. Propofol also induced relaxation of rings precontracted by U46619 in an endothelium-independent way. Propofol at different concentrations significantly inhibited the $Ca^{2+}$-induced contractions of pulmonary rings exposed to high $K^+$-containing and $Ca^{2+}$-free solution in a dose-dependent manner. Propofol relaxed vessels precontracted by the high $K^+$ solution and U46619 in an endothelium-independent way. The mechanism for this effect may involve inhibition of calcium influx through voltage-operated calcium channels (VOCCs) and receptor-operated calcium channels (ROCCs).

• 수산경영론집
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• 제18권1호
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• pp.99-114
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• 1987

연어의 미국 양육은 과거 5년동안 평균 300,000톤으로 비교적 안정적이었다. 반면에 동 기간동안 미국의 연어 수입은 1,800,000파운드에서 19,000,000파운드로 10배나 증가하여 왔다. 이러한 연어의 공급은 70%가 노르웨이에서 이루어졌다. 미국은 1981년까지는 노르웨이로부터 미미한 정도의 연어를 수입하였지만, 1983년에는 1,768톤, 1984년에는 3,869톤 그리고 1985년에는 6,272톤을 수입하였다. 카나다산 연어의 수입가격은 과거 5년전에 $2.58/1b에서 1985년에는 $1.25/1b로 지속적으로 하락하여 왔으나, 반면 노르웨이산 연어의 수입가격은 동일기간동안 $3.28/1b에서 $3.45/1b까지 큰 변동없이 유지되어 왔다. 이와같은 노르웨이산 연어는 1985년에 약 $3.35/1b로 카나다산 연어수입 가격의 약 3배에 달하는 것이다. 미국의 연어의 외형상 소비양은 1981년에 50,000톤이었으나 1985년에는 86%나 증가한 92,000톤으로 급격한 증가현상을 보이고 있다. 특히 매년의 1인당 소비는 1981년에 0.47파운드로부터 1985년에는 0.85파운드로 지속적으로 증가하고 있다. 이상과 같은 상황을 기초로 미래의 연어시장가격을 예측하기 위하여 수요모델을 추정하였다. 추정된 수요모델에 의하면 미국시장에서의 매년의 연어 도매가격은 공급의 증가에 의해서는 하락하지만 미국의 GNP증가에 의해서는 상승할 것이라는 점을 나타내고 있다. 본 연구에 의한 결과는 미국의 총공급양이 25% 증가할지라도 미국의 GNP가 매년 $200,000,000,000씩 증가할 때는 1990년의 연어 도매가격은 1985년 수준으로 유지될 것이라는 점이다.

• Journal of the Korean Wood Science and Technology
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• 제35권5호
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• pp.76-86
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• 2007

This study was conducted to investigate the effects of formaldehyde to urea (F/U) mole ratio on thermomechanical curing of UF resin adhesives with different F/U mole ratios. Thermomechanical curing of these UF resin adhesives was characterized using parameters of dynamic mechanical analysis (DMA) such as the gel temperature, maximum storage modulus, and peak temperatures of storage and loss modulus. As the F/U mole ratio decreased, the gel temperature of UF resin adhesives increased. The maximum storage modulus as an indicator of the rigidity of UF resin adhesives decreased with decreasing F/U mole ratio. The peak temperature of tan $\delta$ increased with decreasing F/U mole ratio, indicating that the vitrification occurred faster for high F/U mole ratio of UF resin adhesives than for the one of lower F/U mole ratio. These results partially explained the reason why UF resin adhesives with lower F/U mole ratio resulted in relatively poor adhesion performance when they were applied.

• Nuclear Engineering and Technology
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• 제34권5호
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• pp.445-454
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• 2002

The sorption of U(Vl) on a domestic granite is studied as a function of experimental conditions such as contact time, solution-solid ratio, ionic strength, and pH using a batch procedure. The distribution coefficients, $K_{d}$＇s, of U(VI) are about 1-100mL/g depending on the experimental conditions. The sorption of U(VI) onto granite particles is greatly dependent upon the contact time, solution-solid ratio, and pH, but very little is dependent on the ionic strength. It is noticed that an U(VI)-carbonate ternary surface complex can be formed in the neutral range of pH. In the alkaline range of pH above 7, U(VI) sorption onto granite particles is greatly decreased due to the formation of anionic U(VI)-carbonate aqueous complexes.s.

• East Asian mathematical journal
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• 제17권2호
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• pp.225-232
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• 2001

Let R be a prime ring with characteristic not two. U a (${\sigma},{\tau}$)-left Lie ideal of R and d : R$\rightarrow$R a non-zero derivation. The purpose of this paper is to invesitigate identities satisfied on prime rings. We prove the following results: (1) [d(R),a]=0$\Leftrightarrow$d([R,a])=0. (2) if $(R,a)_{{\sigma},{\tau}}$=0 then $a{\in}Z$. (3) if $(R,a)_{{\sigma},{\tau}}{\subset}C_{{\sigma},{\tau}}$ then $a{\in}Z$. (4) if $(U,a){\subset}Z$ then $a^2{\in}Z\;or\;{\sigma}(u)+{\tau}(u){\in}Z$, for all $u{\in}U$. (5) if $(U,R)_{{\sigma},{\tau}}{\subset}C_{{\sigma},{\tau}}$ then $U{\subset}Z$.

• Journal of Chest Surgery
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• 제19권1호
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• pp.35-42
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• 1986

Prevention of thrombombolism after rosthetic cardiac valve replacement is essential for the patients. About 90% of patients are free of major and minor thromboembolic complications 5 year after replacement of cardiac valves with prosthetic devices when they are under control of anticoagulant therapy. Ticlopidine is a drug that alter platelet function to have an antithrombotic effect. It is an antiaggregating agent which inhibits primary platelet function to have an antithrombotic effect. It is an antiaggregating agent which inhibits primary platelet aggregation induced by ADP and increases the production of prostaglandin $D_{2}$. Aspirin in small doses inhibits platelet synthesis of prostaglandins by irreversibly blocking the enzyme cyclo-oxygenase. Platelet secretion and aggregation are impaired with Ticlopidine and Aspirin. the thromboembolic event sof 54 patient s who were treated with Ticlopidine and Aspirin after cardiac valve replacement were evaluated and compared with that of 79 patients who were treated with Wafarin and Aspirin after the same type of operation. The follow-up period ranged from 4 to 110 months (mean of 48 months). there were 11 major thromboembolic episodes including three deaths in the warfarin goup during mean follow-up period of 56 months. two cases of CVA and one hemoarthrosis were noted due to overdose of Warfarin. Inticlopidine group, there was only one fatal thromboembolic epdisode three month after mitral valve replacement during mean follow-up period of 18 months. Two episodes of hypermenorrhea resulting anemia ere noted in the ticlopidine group. We measured the parameters of platelet function in aggreagation curve of platelet with platelet aggregometer (chrono-log Aggregometer, Model No. 430) Aggregation test was performed with three final concentrations of epinephrine in 10 uM/L, ADP in 5uM/L. 28 patients with prosthetic cardiac valves and 35 healthy volunteers were subgrouped as follows to analyze the effect of antithrombotic drugs used. Group I ; 11 patients treated with 250-500 mg of ticlopidine and 0.5gm of Aspirin as a daily single dose after cardiac valve replacement (14 St. Jude Medical and 1 Carpentier-Edwards, 9 patients with atrial fibrillation among them) Group II ; 10 patients treated with 3-5 mg of Warfarin and 0.75 gm of Aspirin daily to prolong prothrombin time around 20 seconds for more than 6 months and single Aspirin dose was maintained afterward as a life-long regimes(3 St. Jude Medical, 1 Hall-Kaster and 7 Carpentier-Edwards valve, 9 patients in atrial fibrilation). Group III ; 7 patients who quit anticoagulant treatment (Warfarin + Aspirin) 6-12 months after the regime as group II (3 St. Jude Medical. 1 bjork-Shiley, 1 Hall-Kaster, 3 Carpentier-Edwards valve, 2 of them are with atrial fibrillation). Group IV ; 35 healthy vounteers (28 males and 7 females). The following results were obtained. 1. The mean maximal platelet aggregability in Group I induced by 10uM/L epinephrine was 15.6%, and 17.5 and 18.7% in BM in proportion to the induction by 5 and 10 uM/L ADP. 2. The mean maximal platelet aggregability in Group II induced by 10uM/L epinephrine was 16.5%, and 27.4 and 44.7% in BM in proportion to the induction by 5 and 10uM/L ADP. 3. The mean maximal platelet aggregability in group III induced by 10uM/L epinephrine was 65%, and 56.5 and 51.8% in BM in proportion to the induction by 5 and 10 uM/L ADP. 4. The mean maximal platelet aggregability in the normal subjects induced by 10 uM/L epinephrine was 64%, and 65 and 69% in Bm inproportion to the induction by 5 and 10 uM/L ADP. 5. Reversible change of platelet aggregation curve induced by 5 and 10uM/L was noted all of the patients in Group I. conclusion : Ticlopidine is an antiaggregating agent which inhibits primary platelet aggregation induced by ADP, and increases the production of prostaglandin $D_{2}$. Ticlopidine and Aspirin produced a significant inhibition of platelet in the presence of ADP and epinephrine in our study. Acccording to our brief experience, 250 mg of ticlopidine and low dose of Aspirin resulted synergistic superior effect to each drug alone in prevention of thromboembolism after prosthetic cardiac valve replacement.