• 대한본초학회지
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• 제32권5호
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• pp.7-12
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• 2017

Objectives : Recently, numerous studies reported that excessive generation of advanced glycation end products (AGEs) stimulated expression of skin wrinkle related proteins. This study aimed to evaluate inhibits skin wrinkle formation effect of Mori Folium (MF) through decreased AGEs. Methods : To evaluate the skin wrinkle inhibition effect of MF, SD-rats were divided into three groups; normal rats (Nor), AGEs-induced rats (Con), AGEs-induce rats treated with MF at dose of 100mg/kg body weight (MF). To induced AGEs, streptozotocin (50mg/kg) was injected intraperitoneally, and after 3 days, 100mM methyl glyoxal was administered orally for 3 weeks. After the experiment, the animal's dorsal skin tissues and serum were separated and tested. Results : The oral administration of MF suppressed the AGEs level in serum. Also, the AGEs in skin tissues was significantly reduced through treatment of MF compared with control group. Moreover, the expressions of AGEs related proteins such as polyclonal anti-$N^e$-(carboxymethyl) lysine (CML), anti-$N^e$-(carboxyethyl)lysine (CEL), AGE receptors (RAGE) were reduced in MF group compared with the control group in kidney and skin tissues. The matrix metallo proteinase-1 (MMP-1) reduced by MF treatment with the result that collagen type 1 alpha 2 (COL1A2) was improved that reduced by accumulation of AGEs. Conclusion : The evidence of this study indicate that oral administration of MF reduces the levels of AGEs in serum, skin, and kidney tissues. In conclusion, MF inhibit skin wrinkle formation, suggesting the potential of anti-wrinkle material.

• Human Ecology Research
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• 제59권1호
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• pp.13-21
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• 2021

In order to examine the changes in the appearance properties and the post-adhesion appearance properties of Hanji yarn blended fabrics : 100% Hanji yarn fabric, two kinds of cotton / Hanji yarn blended fabrics and 100% cotton fabric, were selected and fused with three kinds of interlinings. After fusing, changes of standardized KES values were examined. 1. W/T, B/W of Hanji yarn blended fabrics was higher than that cotton fabric. WC/W, 2HB/W, 2HB/B, and 2HG/G values of Hanji yarn blended fabrics are lower than cotton fabric. This means that the Hanji yarn was mixed, shape retention, wrinkle recovery was improved, and the drape property was lowered. 2. After fusing, W/T, shape retention, wrinkle recovery of Hanji yarn blended fabrics increased, and WC/W values of Hanji yarn blended fabrics decreased. The wrinkle recovery property of Hanji yarn blended fabrics were improved; however, the 2HG/G value of Hanji yarn fabric increased due to fusing, and the wrinkle recovery property of Hanji yarn fabric decreased. 3. In the selection of adhesive core, I1 adhesive core is excellent in terms of shape stability and wrinkle recovery; however, an I3 adhesive core is recommended for drape and silhouette formation. When the fabric of the adhesive core was PET, it was found to penetrate better between the fabrics during adhesion than the case of cotton fabrics.

• Applied Microscopy
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• 제41권2호
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• pp.129-137
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• 2011

홍차함유 누드팩의 피부주름 형성 억제효과를 알아보기 위해 SKH-1 hairless 마우스 등 부위에 UVB를 5주간 조사하여 주름을 유발시키고 이와 병행하여 홍차함유 누드팩을 도포한 실험에서 다음과 같은 결과를 얻었다. (1) 육안적인 피부주름 양상 비교에서, 대조군은 정상군에 비해 주름 능선의 두께가 굵고 간격이 넓으며 주름이 깊게 형성된 반면, retinoic acid를 도포한 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 상대적으로 주름 능선의 두께가 얇고 간격이 좁은 양상을 띠었으며 주름 총넓이 수치가 유의하게(p<0.001) 낮았다. (2) 피부의 조직학적 관찰에서, 대조군은 정상군에 비해 진피층내 교원섬유의 배열이 불규칙하고 양이 많이 줄어들었으며 변성된 탄력섬유가 군데군데 엉겨 있는 탄력섬유증이 관찰된 반면, 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 상대적으로 교원섬유의 배열이 규칙적이고 밀도가 조밀하며 변성된 탄력섬유의 양이 줄어 있었다. (3) 피부조직의 MMP-3 mRNA 발현량 측정 결과, 정상군에 비해 대조군은 유의하게(p<0.001) 높았으며 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 유의하게(p<0.001) 낮았다. MMP-2와 MMP-9 단백질 활성 측정 결과, 대조군은 정상군에 비해 현저하게 높은 반면, 양성대조군과 홍차함유 누드팩 도포군은 대조군보다 현저하게 낮게 나타났다. 본 연구를 통하여, 홍차함유 누드팩 도포군은 hairless 마우스에서 세포외 기질단백질의 분해 억제와 피부주름 형성 억제효과를 보였음을 확인하였다.

• Toxicological Research
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• 제33권2호
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• pp.125-134
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• 2017

The effects that ultraviolet rays elicit on collagen synthesis and degradation are the most common causes of wrinkle formation and photo-aging in skin. The objectives of this study were to evaluate the effects of Angelica acutiloba root ethanol extract (AAEE) to promote collagen synthesis and inhibit collagen degradation in human dermal fibroblasts. By examining total polyphenol and flavonoid contents, electron donating ability, radical scavenging activity, and superoxide dismutase-like activity, we found that AAEE exhibited fairly good antioxidant activity. Treatment with AAEE significantly increased type I procollagen production by cultured fibroblasts, as well as reduced ultraviolet-induced matrix metalloproteinase-1 (MMP-1) expression and MMP-2 activity in a dose-dependent manner (p < 0.05). In addition, AAEE significantly increased TIMP-1 mRNA expression (p < 0.05), although without an associated dose-dependent increase in TIMP-1 protein expression. In summary, we suggest that AAEE may be a potentially effective agent for the prevention or alleviation of skin-wrinkle formation induced by ultraviolet rays.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2007년도 Proceedings of The Convention
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• pp.93-114
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• 2007

Vaccinium uliginosum L. (VU) is a flowering plant in the genus Vaccinium has berry fruit. This study was performed to investigate the effect of water extract of Vaccinium uliginosum L. and fractions on inhibition of melanogenesis and wrinkle formation. One hundred grams of the Vaccinium uliginosum L. was extracted with 2,000 mL of water ($90^{\circ}C$, 16h, 2 times). The water extracts were lyophilized and stored at $4^{\circ}C$ until used. Vaccinium uliginosum L. extracts showed scavenger activities on DPPH radical, superoxide anion radical, hydroxyl radical, hydrogen peroxide and singlet oxygen radical, dose dependently. And VU extract and fractions reduced melanin contents on B16F10 melanoma and inhibited the expression of melanogenesis-related proteins, tyrosinase, tyrosinase-related protein (TRP-1) and dapachrometa utomerase (Dct, TRP-2). Moreover VU extract and fractions stimulated procollagen production and inhibited MMP-1 production in human fibroblast. And it decreased degree of wrinkle formation in hairless mouse skin that induced by DVB irradiation for 9 weeks. From the above results, it is possible that Vaccinium uliginosum L. may be developed to be the health functional food and functional cosmetics that have anti-melanogenesis and anti-wrinkle effect.

• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.245-253
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• 2012

Collagen tripeptide (CTP) is a functional food material with several biological effects such as improving dry skin and wound and bone fracture healing. This study focused on the anti-photoaging effects of CTP on a hairless mouse model. To evaluate the effects of CTP on UVB-induced skin wrinkle formation in vivo, the hairless mice were exposed to UVB radiation with oral administration of CTP for 14 weeks. Compared with the untreated UVB control group, mice treated with CTP showed significantly reduced wrinkle formation, skin thickening, and transepidermal water loss (TEWL). Skin hydration and hydroxyproline were increased in the CTP-treated group. Moreover, oral administration of CTP prevented UVB-induced MMP-3 and -13 activities as well as MMP-2 and -9 expressions. Oral administration of CTP increased skin elasticity and decreased abnormal elastic fiber formation. Erythema was also decreased in the CTP-treated group. Taken together, these results strongly suggest that CTP has potential as an anti-photoaging agent.

• 한국식품영양과학회지
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• 제39권2호
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• pp.186-192
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• 2010

피부의 주름은 노화과정의 결과로 유도되어지는데, 비효소적 glycation 반응은 주름생성의 원인 중에 하나로 단백질의 비가역적 가교결합에 의해 생성된 최종당화산물(AGEs)이라고 불리는 갈색화합물을 생성한다. 비교적 반감기가 긴 collagen은 쉽게 glycation이 일어나고 collagen의 가교결합(cross-linking)이 일어나면서 여러 단계의 반응을 거쳐 최종적으로 생성된 AGEs가 피부에 축적되어 피부의 탄력감소 및 주름을 일으킨다고 알려져 있다. 피부 내에 glycation된 collagen의 축척은 피부의 탄력성을 유지하는 것을 방해한다. 수종의 식용작물로부터 항주름소재를 개발하기 위하여 메탄올 및 열수추출물을 조제한 후 AGEs 형성을 억제하는 활성을 검색하였다. 먼저 서양향신료 중에서는 월계수잎, 계피, 정향, 오레가노, 로즈마리, 사보리, 팔각과 한약재 중에서는 복분자, 달개비, 삼지구엽초, 가자와 해당화의 메탄올 및 열수추출물에서 최종당화산물 생성 억제활성이 높았다. 그러나 국내 식물 중에서는 단지 참취 및 녹차 추출물의 메탄올과 열수추출물만이 높은 활성을 보였으며 해조류 중에서는 활성이 높은 시료는 없었다.

• 대한화장품학회지
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• 제32권4호
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• pp.227-231
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• 2006

주름생성의 주요 원인은 자외선, 공기 오염, 담배 연기, 스트레스 등을 들 수 있다. 특히, 자외선은 피부에 다양한 형태로 영향을 주어 깊은 주름, 잔주름, 피부거침, 피부건조와 같은 현상을 발생시켜 피부노화를 유발시킨다. 이러한 주름 생성 해결 및 피부 노화 문제의 해결은 화장품에서 가장 활발하게 연구되는 분야 중 하나이다. 최근에 열대 식물인 노니로부터 추출한 유효성분이 주름 개선에서 가장 중요한 콜라겐 생합성을 촉진하는 효과를 확인하였으며, 특히 노니 추출물로부터 분리한 스코폴레틴(scopoletin)이 이러한 효과를 주는 활성 성분임을 확인하였다. 스코폴레틴은 인체 섬유아 세포를 이용한 콜라겐 합성 촉진 효과 시험에서 농도 의존적으로 콜라겐 합성을 촉진하는 활성을 보였다($0.2{\mu}g/mL-89.5%$ 콜라겐 생합성 촉진. 또한, 주름 개선 효과를 확인하기 위해 3% (v/v) 노니 추출물을 함유한 크림을 이용하여 12주 동안 임상연구를 수행하여, 안면의 주름 감소 정도를 측정하였으며 이 제품이 주름을 감소시켜주는 뛰어난 효과가 있다는 것을 확인하였다. 이러한 결과들은 노니 추출물이 주름 개선효과가 매우 높으며, 노화 방지 원료로서 이용 가능성이 매우 높다는 것을 보여주는 것이라 할 수 있다.

• 한국식품과학회지
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• 제52권4호
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• pp.385-395
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• 2020

This study examined the effect of 3,4-dihydroxytoluene (DHT) on UVB-induced photoaging and determined its molecular mechanisms, using HaCaT human keratinocytes and SKH-1 hairless mice. DHT suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression in HaCaT cells. In vivo data from mouse skin supported that DHT decreased UVB-induced wrinkle formation, epidermal thickness, and matrix metalloproteinase-13 (MMP-13) expression. DHT appeared to exert its anti-aging effects by suppressing UVB-induced Raf-1 kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK, ERK, and p90RSK in HaCaT cells. In vitro and in vivo pull-down assays revealed that DHT bound with Raf-1 in ATP-noncompetitive manner. Overall, DHT appears to anti-photoaging effects in vitro and in vivo through the suppression of Raf-1 kinase activity and may have potential as a treatment for the prevention of skin aging.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.219-238
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• 2003

Indole-3-acetic acid (IAA) molecule has been successfully encapsulated in biocompatible inorganic matrix in order for the cosmetic application such as superfacial fine line reduction. The encapsulation was realized through chemical reaction involving simultaneous formation of inorganic lattice and 1M giving rise to an 1M-inorganic nanohybrid (IAA-brid) which shows excellent storage stability and sustained releasing property of indole-3-acetic acid. The clinical efficacy of essence cream containing IAA-brid as anti-wrinkle formulation was also carefully evaluated by measuring the roughness of the skin replica before and after treatment. Upon administration of the cream on the eye-area, the fine-line is drastically reduced.