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http://dx.doi.org/10.9721/KJFST.2020.52.4.385

3,4-Dihydroxytoluene suppresses UVB-induced wrinkle formation by inhibiting Raf-1  

Park, Sang-Hee (School of Food Science and Biotechnology, Kyungpook National University)
Kang, Nam Joo (School of Food Science and Biotechnology, Kyungpook National University)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.4, 2020 , pp. 385-395 More about this Journal
Abstract
This study examined the effect of 3,4-dihydroxytoluene (DHT) on UVB-induced photoaging and determined its molecular mechanisms, using HaCaT human keratinocytes and SKH-1 hairless mice. DHT suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression in HaCaT cells. In vivo data from mouse skin supported that DHT decreased UVB-induced wrinkle formation, epidermal thickness, and matrix metalloproteinase-13 (MMP-13) expression. DHT appeared to exert its anti-aging effects by suppressing UVB-induced Raf-1 kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK, ERK, and p90RSK in HaCaT cells. In vitro and in vivo pull-down assays revealed that DHT bound with Raf-1 in ATP-noncompetitive manner. Overall, DHT appears to anti-photoaging effects in vitro and in vivo through the suppression of Raf-1 kinase activity and may have potential as a treatment for the prevention of skin aging.
Keywords
3,4-dihydroxytoluene; Raf-1; UVB; photoaging;
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