Anti-skin-aging Effect of Mori Folium through decreased Advanced glycation end product (AGEs)

최종당화산물 억제를 통한 상엽(桑葉)의 항피부노화 효과

  • Lee, AhReum (College of Korean Medicine, Daegu Haany University) ;
  • Kim, SooHyun (College of Korean Medicine, Daegu Haany University) ;
  • Kim, SuJi (College of Korean Medicine, Daegu Haany University) ;
  • Kim, KyeongJo (College of Korean Medicine, Daegu Haany University) ;
  • Kwon, Ojun (Gyeongbuk Institute for Regional Program Evaluation) ;
  • Choi, JoonYoung (Sae Earl Bio Food co., Ltd) ;
  • Roh, Seong-Soo (College of Korean Medicine, Daegu Haany University)
  • 이아름 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수지 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김경조 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 권오준 (경북지역사업평가단) ;
  • 최준영 (농업회사법인 새얼 바이오푸드 주식회사) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2017.07.26
  • Accepted : 2017.09.15
  • Published : 2017.09.30


Objectives : Recently, numerous studies reported that excessive generation of advanced glycation end products (AGEs) stimulated expression of skin wrinkle related proteins. This study aimed to evaluate inhibits skin wrinkle formation effect of Mori Folium (MF) through decreased AGEs. Methods : To evaluate the skin wrinkle inhibition effect of MF, SD-rats were divided into three groups; normal rats (Nor), AGEs-induced rats (Con), AGEs-induce rats treated with MF at dose of 100mg/kg body weight (MF). To induced AGEs, streptozotocin (50mg/kg) was injected intraperitoneally, and after 3 days, 100mM methyl glyoxal was administered orally for 3 weeks. After the experiment, the animal's dorsal skin tissues and serum were separated and tested. Results : The oral administration of MF suppressed the AGEs level in serum. Also, the AGEs in skin tissues was significantly reduced through treatment of MF compared with control group. Moreover, the expressions of AGEs related proteins such as polyclonal anti-$N^e$-(carboxymethyl) lysine (CML), anti-$N^e$-(carboxyethyl)lysine (CEL), AGE receptors (RAGE) were reduced in MF group compared with the control group in kidney and skin tissues. The matrix metallo proteinase-1 (MMP-1) reduced by MF treatment with the result that collagen type 1 alpha 2 (COL1A2) was improved that reduced by accumulation of AGEs. Conclusion : The evidence of this study indicate that oral administration of MF reduces the levels of AGEs in serum, skin, and kidney tissues. In conclusion, MF inhibit skin wrinkle formation, suggesting the potential of anti-wrinkle material.



  1. Park SK, Kim SN, Lee JC, Kim HS, Kim YJ, Lee BG, Chang IS. Anti-aging effet on skin with Jaeum-Dan (JED). Kor. J. Herbology. 2004 : 19(1) ; 67-76.
  2. Gkogkolou P, Bohm M. Advanced glycation end products: Key players in skin aging?. Dermatoendocrinology. 2012 ; 4(3) : 259-70.
  3. Park SJ, Chung H. Outer blood-retinal barrier alteration induced by intraocular advanced glycation endproduct. J. Korean Ophthalmol. Soc. 2001 ; 42(2) : 151-5.
  4. Kwon HO, Lee MH, Kim YJ, Kim E, Kim OK. Beneficial effects of Acanthopanax seticosus extract in type II diabetes animal model via down-regulation of advanced glycated hemoglobin and glycosylation end products. J. Korean Soc Food Sci Nutr. 2016 ; 45(7) : 929-37.
  5. Kim BH, Son SM. Mechanism of Developing Diabetic Vascular Complication by Oxidative Stress. Endocrinology and Metabolism 2006 ; 21(6) : 448-59.
  6. Jeon SH, Yi DH. Effects of Alliin on Cellular Protection, Up-regulation of Collagen and Downregulation of MMP1 in Human Dermal Fibroblasts Kor. J. Aesthet. Cosmetol., 2014 ; 12(2) : 248-58.
  7. Ana PCS, Carissa AG, Eduardo AGR, Renan FT, Ludmila FV, Tuania SC, Maria CTC, Antonio LBP, Jean NS. Infrared LED light therapy influences the expression of fibronectin and tenascin in skin wounds of malnourished rats-A preliminary study. Acta Histochem. 2014 ; 116 : 1185-91.
  8. Kim KB, Jo A, Cha HJ, Shin SH, Lee JJ, Lee HK, An IS. Synergetic effects of sericin and alphamangostin on anti-wrinkle effects. Kor. J. Aesthet. Cosmetol. 2015 ; 13(6) : 729-34.
  9. Kim YC, Park EY, Kim SN, Yoo YG, Park MS, Lee GY, Lee SJ, Chang BS. Inhibitory effects of nude pack containing black tea water extract on skin wrinkle formation in hairless mice. Korean. J. Microsopy. 2011 ; 41(2) : 129-37.
  10. Ju MJ, Kwon JH, Kim HK. Physiological activities of Mulberry leaf and fruit extracts with different extraction conditions. Korean. J. Food Preserv. 2009 ; 16(3) : 442-8.
  11. Kwon OJ. Effect of Mori Follium extract on the melanogenesis and skin fibril matrix. Kor. J. Herbology. 2016 ; 31(5) : 41-6.
  12. Kweon KT. A research on management system of herbal medicine in common use for food and medicine. Kor. J. Herbology. 2012 ; 27(2) : 25-9.
  13. Naowaboot J, Pannangpetch P, Kukonqviriyapan V, Kongyingyoes B, Kukonqviriyapan U. Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract in streptozotocininduced chronic diabetic rats. Plant Foods Hum Nutr. 2009 ; 64 : 116-21.
  14. Hunyadi A, Veres K, Danko B, Kele Z, Weber E, Hetenyi A, Zupko I, Hsieh T. In vitro anti-diabetic activity and chemical characterization of an apolar fraction of Morus alba leaf water extract. Phytother Res. 2013 ; 27(6) : 847-51.
  15. Park E, Song JH, Kim GN, Kim HO. Anti-oxidant and anti-obese effects of Mulberry (Morus alba L.) leaf extract in 3T3-L1 cells. Kor. J. Aesthet. Cosmetol. 2015 ; 13(1) : 19-26.
  16. Lee HS, Yoon JA. Inhibitory activity of advanced glycation end products(AGE) formation of edible plants for development of anti-wrinkle ingredients. J. Korean Soc. Food Sci Nutr. 2010 ; 39(2) : 186-92.
  17. Singh R, Barden A, Mori T, Beilin L. Advanced glycation end products: a review. Diabetologia. 2001 ; 44(2) : 129-46.
  18. Kim SJ, Lee AR, Kim SH, Kim KJ, Kwon OJ, Choi JY, Koo JS, Roh SS. Skin wrinkle improvement effect of Paeoniae radix and processed Paeoniae radix through inhibition of advanced glycation end products (AGEs). Kor. J. Herbol. 2017 ; 32(4) : 53-60.
  19. Kim SJ, Lee YK, Lee HJ, Kim JS. The Effects of LR3 and SP6 Acupuncture on Liver Damage of Streptozotocin-induced Diabetic Mice. The Acupuncture. 2016 ; 33(3) : 29-43
  20. MacNaul KL, Chartrain N, Lark M, Tocci MJ, Hutchinson NI. Discoordinate expression of stromerylsin collagenase, and tissue inhibitor of metalloproteinases-1 in rheumatoid human synovial fibroblasts: synergistic effects of interleukin-1 and tumor necrosis factor-alpha on stromelysin expression. J Biol Chem 1990 ; 265(28) : 17238-145.