• 제목/요약/키워드: Wax gourd

검색결과 31건 처리시간 0.026초

Characterization of Soluble Dietary Fibers from Wax Gourd (Benincasa hispida) Pulp and Peel

  • Hong, Sun-Pyo;Jun, Hyun-Il;Song, Geun-Seoup;Kwon, Yong-Ju;Kim, Young-Soo
    • Food Science and Biotechnology
    • /
    • 제17권4호
    • /
    • pp.734-738
    • /
    • 2008
  • The physicochemical and in vitro physiological properties of soluble dietary fiber (SDF) from wax gourd (Benincasa hispida) pulp and peel were investigated. The pulp was composed of 11.4% SDF and 24.3% insoluble dietary fiber (IDF), while the peel contained 3.2% SDF and 43.3% IDE The predominant sugar in the SDF of the wax gourd pulp and peel was uronic acid, followed by galactose and rhamnose. The SDFs from the wax gourd pulp and peel gave similar elution patterns, with 4 main neutral sugar and uronic acid peaks eluted by 0.4, 0.5, 1, and 2 M ammonium acetate buffer. The pulp SDF had a much higher glucose retardation index (GRI) than the peel SDF for all measurement times. The pulp SDF showed strong growth-inhibiting activity against Escherichia coli and Clostridium perfringens, whereas the peel SDF produced strong growth-promoting activity against Bifidobacterium longum, Bifidobacterium infantis, and Lactobacillus brevis when compared to glucose.

동아의 체중조절 효과 (Weight Loss Effect of Wax Gourd)

  • 홍석산;이승환;김철용;권석형;황보식
    • 한국식품영양학회지
    • /
    • 제15권4호
    • /
    • pp.289-294
    • /
    • 2002
  • 본 연구에서 선발된 17종의 천연물을 Sprague-Dawleyra rat에 급여한 결과, 체중증가, 에너지 소비, fat pads 중량, 간의 triglyceride함량, atherogenic index 그리고 혈중 글루코오스의 함량 등이 감소하는 경향을 나타내었다. 특히 동아를 구강으로 섭취시킨 결과, 체중증가, 에너지 소비, fat pads 중량, 그리고 혈중 글루코오스의 함량 등이 감소하였으나, 간의 triglyceride 함량은 오디와 송엽의 열수 추출물보다 적게 감소하였다. 동아, 오디, 그리고 송엽을 혼합하여 사용한 결과, 각각의 원효를 사용할 경우보다 비만이 개선되는 것으로 나타났다.

Wax Gourd Reduces Adipose Depots and Fat Cell Sizes without Affecting the Levels of Leptin in Rats Fed Medium Fat

  • Kang Keum-Jee
    • Nutritional Sciences
    • /
    • 제9권3호
    • /
    • pp.173-178
    • /
    • 2006
  • The purpose of this study was to investigate the effects of wax gourd on body weight, body fat, triglyceride, leptin and fat cell size in rats fed medium fat Male Sprague Dawley rats were fed with experimental diet containing total dietary fat at 20% of calorie with wax gourd (WG) for 4 weeks. Forty rats were divided into 4 groups including control, 5, 10, and 15% (w/w) WG supplement The results showed that weight gain was significantly higher in 5% WG than that in control (p<0.05), but was not different among the WG supplement groups. Total fat weight was significantly decreased in both 10 and 15% WG supplement groups than that of control. The levels of triglycerides and free fatty acids in the plasma were significantly decreased, particularly in 15% WG supplement group (p<0.05). The levels of leptin was not significantly different among the experimental groups. Fat cell size was significantly decreased in WG supplement groups compared to that of control group (p<0.05). Correlation among the parameters demonstrated that weight gain correlated positively with total fat weight, the levels of leptin and triglycerides. Taken these results together, 15% WG supplement diet substantially reduces total fat weight, triglycerides and free fatty acids levels and fat cell size in the rat fed midium fat.

동아 추출물과 프로바이오틱 유산균의 생리활성 및 과민성대장증후군 개선 효과 (Biological Activity and Improvement Effect on Irritable Bowel Syndrome of Wax Gourd Extract and Probiotic Lactic Acid Bacteria)

  • 안용근;장병철;박세준
    • 한국식품영양학회지
    • /
    • 제26권1호
    • /
    • pp.137-145
    • /
    • 2013
  • 동아(冬瓜, wax gourd, Benincase hispida) 추출물과 프로바이오틱 유산균(Lactobacillus casei와 Bifidobacterium bifidum)(이하 유산균이라 한다)의 생리활성을 분석하였다. 동아 추출물은 안기오텐신전환효소의 활성을 47.9%, 티로시나제의 활성을 13.2% 저해하였고, 항산화 활성을 23.4% 나타냈다. 설사 증세자 120명에게 동아 추출물 제제를 72시간 복용시킨 결과 117명이 개선되었고, 6~12시간째에 개선율이 가장 높았다. 개선율은 80%가 치료되는 상태를 기준하였다. 유산균 제제는 안기오텐신전환효소의 활성을 21.4% 저해하였다. 설사 증세자 120명에게 유산균 제제를 72시간 복용시킨 결과 108명이 개선되었고, 24시간째에 개선율이 가장 높았다. 이들 결과를 바탕으로, 과민성대장증후군 개선을 위한 동아 추출물과 유산균을 함유한 제제(tablet)를 개발하였다. 이 제품은 안기오텐신전환효소의 활성을 27.1% 저해하였고, 항산화 활성을 20.3% 나타냈다. 인체폐암 A549 세포에 100 ${\mu}g/m{\ell}$ 및 250 ${\mu}g/m{\ell}$ 농도로 이 제제를 가하여 24시간 반응시킨 결과, 세포 증식을 67% 억제하였고, 동아 추출물 제제나 프로바이오틱 유산균 제제 단독으로 작용시킨 것보다 억제율이 높았다. 또 이 제제를 인체대장암 HCT116 세포에 100 ${\mu}g/m{\ell}$ 농도로 가한 결과, 24시간 후 세포 증식이 70% 억제되었고, 동아 추출물 제제나 유산균 제제 단독으로 작용시킨 것보다 개선율과 개선 속도가 높았다. 이 제제를 설사형, 변비형, 일반형의 과민성대장증후군 증세자 164명에게 72시간 복용시킨 결과 100% 개선시켰으며, 개선율은 3~6시간째에 가장 높았고, 동아 추출물 제제나 유산균 제제 단독으로 사용한 것보다 개선율과 개선속도가 높았다.

Effects of Wax Gourd Extracts on Adipocyte Differentiation and Uncoupling Protein Genes(Ucps) Expression in 3T3-Ll Preadipocytes

  • Kang, Keun-Jee;Kwon, So-Young
    • Nutritional Sciences
    • /
    • 제6권3호
    • /
    • pp.148-154
    • /
    • 2003
  • Although various raw plant materials have been demonstrated to exert anti-obesity effects to a greater or lesser extent in both humans and animals when they are used to supplement the diet, it has not been shown extensively that they influence adipocyte cell differentiation involving lipid metabolic gene expressions. Using a well-established 3T3-L1 preadipocyte differentiation system, we decided to look into molecular and cellular event occurring during adipocyte differentiation when raw plant materials aye included in the process, in an effort to demonstrate the potential use of a screening system to define the functions of traditionally well-known materials. To these ends, the effects of ethanol (EtOH) or EtOH/distilled water (DW) extracts of Wax Gourd were examined using cytochemical and molecular analyses to determine whether components of the extracts modulate adipocyte differentiation of 3T3-Ll preadipocytes in vitro. The cytochemical results demonstrated that EtOH or EtOH/DW extracts did not affect lipid accumulation and cell proliferation, although the degree of lipid accumulation was influenced slightly depending on the extract. EtOH extract was highly effective in apoptotic induction during differentiation of 3T3-Ll preadipocytes (p<0.05). Reverse transcription-polymerase chain reaction (RT-PCR) analysis of lipoprotein lipase (LPL), Uncoupling protein (Ucp) 2, 3 and 4 also showed that while LPL expression was not influenced, Ucp2, 3 and 4 were up regulated in the EtOH extract-treated group and down regulated in the EtOH/DW extract-treated group. These changes in gene expressions suggest that the components in different fractions of Wax Gourd extracts may modulate lipid metabolism by either direct or indirect action. Taking these results together, it was concluded that molecular and cellular analyses of adipocyte differentiation involving lipid metabolic genes should facilitate understanding of cellular events occurring during adipocyte differentiation. Furthermore, the experimental scheme and analytical methods used in this study should provide a screening system for the functional study of raw plant materials in obesity research.

생녹혈의 건조 및 안정화 (Drying and Stabilization of Deer Blood)

  • 안용근
    • 한국식품영양학회지
    • /
    • 제22권1호
    • /
    • pp.20-28
    • /
    • 2009
  • According to traditional oriental medicine, only non-coagulated native deer blood is said to be effective, and coagulated deer blood is ineffective. Thus, a drying and tablet-producing method for deer blood was developed to maintain its physiological and therapeutic activity, and so that after drying, it can be redissolved and protected from coagulation. Proteases such as trypsin, pepsin, chymotrypsin, and aminopeptidase were added to the deer blood indicating that it coagulated in an hour, as shown by the reference. Wax gourd extract, which is high in protease, was added to the blood resulting in anticoagulation for 31 hours. Also, additions of 1% EDTA, 0.38% sodium citrate, 0.16% calcium oxalate, 1.2% ethanol, and 0.006% heparin to the deer blood resulted in anticoagulation for 1 hour, 4 hours, 2 hours, 1 hour, and 31 hours, respectively. In an experiment using 0.19% sodium citrate plus 1% wax gourd extract, and 0.006% heparin plus 1% wax gourd extract, anticoagulation was maintained for up to 72 hours. However, since heparin can not be used in food, the deer blood tablet was made with the addition of 0.19% sodium citrate and 1% wax gourd extract, followed by freeze drying. The dissolution rate for the tablet manufactured in this manner was 96.7%. And the dissolution rates for spray-dried deer blood, vacuum-dried deer blood, and marketed deer blood tablets were 85%, 81%, and 25.5%, respectively. The composition of the tablet produced from the freeze-dried deer blood was 56.4% protein, 18.7% lactose, 1.2% amino acids, 1.0% glucose, 0.7% lipids, 180 mg/100 g of iron, 13 mg/100 g of potassium, 39.1 mg/100 g of calcium, 480 mg/100 g of sodium, 368 mg/100 g of chloride, each.

동아의 물리적 특성 및 in vitro 포도당, 담즙산, 카드뮴 투과억제 효과에 대한 열처리 영향 (Effect of Heat Treatments on Physical Properties and in vitro Glucose, Bile Acid, and Cadmium Transport Retardation of Wax Gourd (Benincasa hispida))

  • 주인옥;정기태;류정;김영수
    • 한국식품과학회지
    • /
    • 제35권6호
    • /
    • pp.1117-1123
    • /
    • 2003
  • 동아를 autoclaving, boiling, microwaving 처리하고 그에 따른 물리적 특성과 in vitro 생리적 특성의 변화를 조사하였다. 열처리 방법에 따른 보수력은 microwaving이 9.43 g/g으로 가장 높았으며 boiling(5.12 g/g), 무처리(4.63 g/g), autoclaving(2.61 g/g) 순으로 낮아졌다. 팽윤력은 열처리 동아의 경우 $22.4{\sim}25.8\;mL/g$으로 무처리(18.0m L/g)보다 높은 것으로 나타났다. 동아의 calcium 흡착력은 microwaving, boiling, 무처리, autoclaving 순으로 나타났으며 autoclaving의 경우 나머지 처리의 30% 정도의 낮은 흡착력을 나타내었다. 열처리동아의 세포조직은 autoclaving 처리에 의하여 가장 심한 구조적 변형을 보였다. 반투막을 이용하여 in vitro법으로 확인한 glucose 투과 억제효과는 boiling 처리에 한해서 20.6%(투석 1시간)로 나타났다. Bile acid 투과 억제효과는 boiling, autoclaving, microwaving, 무처리 순으로 각각 22.9, 17.1, 14.3, 8.6%(투석 1시간)의 투과 억제효과를 보였다. Cadmium투과 억제효과는 열처리 방법에 관계없이 65% 이상으로 높게 나타났다.

동아와 달개비의 전기영동에 의한 Isozyme의 변이 (Isozyme Patterns on Esterase, Peroxidase and Amylase in Benincasa hipspida (Thunb) Cogn and Commelina communis L.)

  • 채규인;최정식;김영두
    • 한국작물학회지
    • /
    • 제36권2호
    • /
    • pp.174-176
    • /
    • 1991
  • 동아(Benincasa hispida(Thunb) Cogn)와 달개비 (Commelina communis L.)의 종자, 잎 및 줄기등을 공시재료로 하여 몇가지 효소에 대한 전기영동적 특성을 비교한 결과는 다음과 같다 1. Esterase isozyme의 pattern은 band수나 Rf값에서 현저한 차이를 나타냈으며 동아의 육질에서는 단 1개의 band가 출현하였다. 2. Peroxidase isozyme은 동아의 육질에서 4개의 band가 출현하였으나, 달개비의 줄기에서는 band 가 전혀 없었다. 3. Amylase isozyme의 전기영동대는 동아의 종자 및 육질에서만 각각 1개씩 뚜렷한 band를 나타냈다.

  • PDF

구절초진주식초 (Vinegar Produced from Chrysanthemum zawadskii and Pearl Shell)

  • 안용근;오문헌;이병엽
    • 한국식품영양학회지
    • /
    • 제25권1호
    • /
    • pp.90-98
    • /
    • 2012
  • 동과착즙액에 에탄올을 가해 초산발효시켜서 산도 5.0%의 식초를 만들고, 여기에 구절초 추출액(농도 10%)을 10%를 가한 다음, 조개껍질을 녹여서 식초를 제조하였다. 동과 식초에 대하여 꼬막껍질, 굴껍질, 전복껍질을 1% 가하면 97.2~98.4% 녹고, 2% 가하면 95.8~95.6% 녹았다. 조개껍질을 1% 녹인 식초의 산도는 3.00~3.17, 2% 녹인 것은 1.11~1.20였고, 조개껍질을 1% 녹인 식초의 pH는 4.54~4.55, 2% 녹인 식초는 4.86~4.95로 조개껍질 1%짜리의 경우 시판 식초보다 산도가 높으면서도 pH 수치가 높아서 유리산이 적었다. 초산발효시 구절초를 가하면 1%일 경우 저해율 44.4%, 0.12%일 경우 저해율 22.2%을 나타내므로 구절초는 초산발효 후 첨가해야 한다. 조개껍질 1% 식초의 칼슘함량은 0.4%, 2% 식초는 0.78%였다. 열처리하지 않은 동과는 angiotensin 전환효소 저해활성 21.7%, 항산화효소 활성 5.23%, 티로시나제 저해활성 5.5%를 나타냈고, 열처리 추출 동과는 angiotensin 전환효소 저해활성 16.1%, superoxide dismutase 활성 20.5%, 항산화 활성 23.2%, 티로시나제 저해활성 7.1%를 나타냈다. 구절초는 angiotensin 전환효소 저해활성 28.8%, xanthine oxidase 저해활성 28.2%, speroxide dismutase 활성 14.5%, 항산화 활성 3.2%, 티로시나제 저해활성 9.2%로 나타났다. 10% 시료를 인간폐암상피세포주 A549 세포에 가한 결과, 세포는 동과가열추출액의 경우, 72시간에 80%, 생동과는 48시간 뒤에 74%, 구절초 가열 추출액은 72시간 뒤에 100% 암세포를 감소시켜 항암작용을 나타냈다.

동과(Benincasa hispida) 분획물의 투여가 Streptozotocin 유발 당뇨 흰쥐의 항산화 효과에 미치는 영향 (Effects of Fractions of Benincasa hispida on Antioxidative Status in Streptozotocin Induced Diabetic Rats)

  • 임숙자
    • Journal of Nutrition and Health
    • /
    • 제40권4호
    • /
    • pp.295-302
    • /
    • 2007
  • This study was designed to examine the effects of fractions of ethanol extract of Benincasa hispida (wax gourd) on hepatic antioxidative status in streptozotocin (STZ) induced diabetic rats. Sprague-Dawley rats were induced diabetes mellitus by STZ injection (45 mg/kg) into the tail vein and were divided into 5 groups: normal, STZ-control, three experimental diabetic groups. Fractions of ethanol extract of Benincasa hispida were administered orally into the diabetic rats for 14 days. Hepatic glutathione peroxidase (GSH-px) activity (determined with H$_2$O$_2$ as substrate) was increased in the groups supplemented with chloroform (CHCl$_3$) and butanol (BuOH) fractions. Glutathione peroxidase (GR) activity in the liver cytosol of H$_2$O fraction groups was significantly lower than that of STZ-control group. The H$_{2}$O fraction supplemented group has been shown the notably decrease in the hepatic superoxide dismutase (SOD) activity. The hepatic cytosol catalase (CAT) activity was significant decreased by the supplementation with BuOH fraction. It was found from the results that the supplementation of BuOH and H$_2$O fractions of Benincasa hispida extract could be beneficial for the diabetic complications and damages from the lipid peroxidation.