Browse > Article

Effects of Fractions of Benincasa hispida on Antioxidative Status in Streptozotocin Induced Diabetic Rats  

Lim, Sook-Ja (Department of Food and Nutrition, Duksung Women's University)
Publication Information
Journal of Nutrition and Health / v.40, no.4, 2007 , pp. 295-302 More about this Journal
Abstract
This study was designed to examine the effects of fractions of ethanol extract of Benincasa hispida (wax gourd) on hepatic antioxidative status in streptozotocin (STZ) induced diabetic rats. Sprague-Dawley rats were induced diabetes mellitus by STZ injection (45 mg/kg) into the tail vein and were divided into 5 groups: normal, STZ-control, three experimental diabetic groups. Fractions of ethanol extract of Benincasa hispida were administered orally into the diabetic rats for 14 days. Hepatic glutathione peroxidase (GSH-px) activity (determined with H$_2$O$_2$ as substrate) was increased in the groups supplemented with chloroform (CHCl$_3$) and butanol (BuOH) fractions. Glutathione peroxidase (GR) activity in the liver cytosol of H$_2$O fraction groups was significantly lower than that of STZ-control group. The H$_{2}$O fraction supplemented group has been shown the notably decrease in the hepatic superoxide dismutase (SOD) activity. The hepatic cytosol catalase (CAT) activity was significant decreased by the supplementation with BuOH fraction. It was found from the results that the supplementation of BuOH and H$_2$O fractions of Benincasa hispida extract could be beneficial for the diabetic complications and damages from the lipid peroxidation.
Keywords
Benincasa hispida (wax gourd); ethanol extract; diabetic rats; antioxidative status;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Zimmet P, Alberti KGMM, Shaw J. Global and societal implications of the diabetes edemic. Nature 2001; 414: 782-787   DOI   ScienceOn
2 Rhee SJ, Choe WK, Cha BK, Yang JA, Kim KY. Effects of vitamin E and selenium on the antioxidative defense system in streptozotocin induced diabetic rats. J Korean Soc Nutr 1996; 29(1): 22-31
3 Huh J. The Handbook of oriental medicine. Namsandang; 1994. p.1170
4 Abei H. Catalase in vitro. Methods Enzymol 1984; 105: 121-126   DOI
5 Kakkar R, Kalra J, Mantha SV, Prasod K. Lipid peroxidation and antioxidant enzymes in diabetic rats. Mol Cell Biochem 1995; 151: 113-119   DOI   ScienceOn
6 Bang MA, Kim HA, Cho YJ. Hypoglycemic and antioxidant effect of dietary hamcho powder in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2002; 31(5): 840-846   DOI
7 Bus JS, Aust SD, Gibson JE. Superoxide and singjet oxygen catalyzed lipid peroxidation as a possible mechanism for paraquat (methyle viologen) toxicity. Biophys Res Commun 1974; 58: 749-753   DOI   ScienceOn
8 Fridovich I. The biology of oxygen radicals, the superoxide radical is an agent of oxygen toxicity: superoxide dismutase provide an important defense. Science 1978; 201: 875-882   DOI
9 Deisseroth A, Dounce AL. Catalase physical and chemical properties, mechanism of catalysis and physiological role. Physiol Rev 1970; 50: 3-24
10 Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases: The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249: 7130-7139
11 Grover JK, Adiga G, Vats V, Athi SS. Extracts of Benincasa hispida prevent development of experimental ulcers. J Ethnopharmacology 2001; 78: 159-164   DOI
12 Hermenegildo C, Raya A, Roma J, Romero FJ. Decrease glutathione peroxidase activity n sciatic nerve of alloxan-induced diabetic mice and its correlation with blood glucose levels. Neurochem Res 1993; 18: 893-896   DOI
13 Chow CK. Nutritional influence on cellular antioxidant defense system. J Biol Chem 1969; 244: 6049-6054
14 Kwang OG, Rihee SJ. Effects of vitamin E on the oxidative damage and glomerular filtration rates of kidney in streptozotocininduced diabetic rats. Korean J Nutr 2000; 33: 625-631
15 Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium deficient rat liver. Biochem Biophys Res Comm 1976; 71: 952-958   DOI   ScienceOn
16 Park GY, Lee SJ, Im JG. Effects of green tea catechin on cytochrome p450 xanthine oxidase activities in liver and liver damage in streptozotocin induced diabetic rats. J Korean Soc Nutr 1997; 26: 901-907
17 Lim SJ, Lee MH. Effects of fracion of Benincasa hispida on plasma levels of glucose and lipid in streptozotocin induced diabetic rats. Korean J Nutr 2005; 38(10): 801-806   과학기술학회마을
18 Rhee SJ, Choe WK, Cha BK, Yang JA, Kim KY. Effects of vitamin E and selenium on the antioxidative defense system in streptozotocin- induced diabetic rats. Korean J Nutr 1996; 29: 22-31
19 Baynes JW. Role of oxidative stress in the development of complication in diabetes. Diabetes 1991; 40: 405-421   DOI   ScienceOn
20 Maklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47(3): 467-474
21 Yoo SK, Rhee SJ. Effects of YK-209 mulberry leaves on antioxidative defense system of liver in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2002; 31: 1065-1070   DOI
22 Kwag OC, Yang JA, Rhee SJ. Effects of vitamin E on the antioxidative defense system of kidney in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 1997; 28: 651-662
23 Videla LA, Fernandez V. Biochemical aspects of cellular oxidative stress. Arch Biol Med Exp 1988; 21: 85-92
24 Kinalski M, Seledziewski A, Telejko B, Zarzycki W, Kinalska I. Lipid peroxidation and scavenging enzyme activity in streptozotocin- induced diabetes. Acta Diabetol 2000; 37: 179-183   DOI
25 Chai TM, Park MR, Rhee SJ. Effects of green tea catechin on the antioxidative defense system and lipofuscin levels of heart in streptozotocin-induced diabetic rats. Korean J Gerontol 1998; 8: 96-103
26 Wobaieb SA, Godin DV. Alterations in tissue antioxidant systems in the spontaneously diabetic (BB Wistar) rat. Can J Physiol Pharmacol 1987; 65(11): 2191-2195   DOI
27 Warier PK. Indian medicinal plants. Orient Longman Limited, India; 1994. p.261
28 Borrpart GJ, Prevot DS, Bascands JL. Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: application to cisplatin-induced toxicity. Clin Biochem 1990; 23: 501-504   DOI   ScienceOn
29 Lee SS, Kim JW. Pharmacological studies on the water extract of fractus of Lycium chinese Mill. Duksung Bull Pharm Sci 1991; 2: 29-41
30 Reeves PG. Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 1997; 127: 838-841   DOI
31 Lee YR, Kang MY, Nam SH. Effect of giant embryonic rice supplementation on the lipid peroxide levels and antioxidative enzyme activities in the plasma and liver of streptozotocin-induced diabetic rats. J Korean Soc Appl Biol Chem 2005; 48(4): 358-363
32 Wolff SP, Jiang ZY, Hung JV. Protein glycation and oxidative stress in diabetes mellitus and aging. Free Radical Biol Med 1991; 10: 339-352   DOI   ScienceOn
33 Halliwell B, Gutteridge MC. Free radicals in biology and medicine. Oxford University Press Oxford; 1985. p.166-170
34 Lowry OH, Rosebrough NJ, Farr AJ, Randall RR. Protein measurement with the foline phenol reagent. J Biol Chem 1951; 193: 265-273
35 Lim SJ, Lee MH. Effects of Benincasa hispida fraction on hepatic lipid levels and lipid peroxidation in streptozotocin induced diabetic rats. Korean J Nutr 2006; 39(6): 513-519   과학기술학회마을
36 Awasthi YC, Beutler E, Srivstava SK. Purification and properties of human erythrocyte glutathione peroxidase. J Biol Chem 1975; 250: 5144-5149
37 Sharma LK. Food medicines. Practical nature cure. Natural Cure Publishing House, Pudukkottai, India; 1984. p.169
38 Bergmeyer HU, Gawehn K, Grassl M. In methods of enzymatic analysis (Bergmeter. HU Ed.). 2nd ed. Vol 1. Academic Press Inc. New York; 1974
39 Duke EJ, Joyce P, Ryan JP. Characteriztion of alternative molecular forms of xanthine oxidase in the mouse. J Biochem 1973; 131: 187
40 Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol Rev 1979; 59: 527-552   DOI
41 Hammers HD. Aminoguanidine treatment inhibits the development of edible and medicinal diabetic retinopaty. Proc Nat Acad Sci USA 1991; 88: 11555-11558
42 Ramanathan M, Jaiswal AK, Bhattacharya SK. Superoxide dismutase, catalase and glutathione peroxidase activities in the brain of streptozotocin induced diabetic rat. Indian J Exp Biol 1999; 37(2): 182-183
43 Jang YS, Ahn HS, Kim HR. Effects of vitamin E supplementation on the lipid peroxides and activities of antioxidative enzymes in the pancrease of diabetic KK mice. Korean J Nutr 1998; 31: 153-158
44 Mavis RD, Stellwagen E. Purification and subunit structure of glutathione reductase from baker's yeast. J Biological Chemistry 1968; 243(3): 809-814
45 Park YR, Rhee SJ, Lin S, Joo GJ. Effects of dietary vitamin E on the microsomal mixed function oxidase system of liver and lung in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 1996; 25(6): 969-975
46 Kim MJ, Cho SY, Lee MK, Shin KH. Effects of Aralia elata water extracts on activities of hepatic oxygen free radical generating and scavenging enzymes in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2004; 33(4): 653-658   DOI
47 Mekinova D, Chorvathova V, Volkovova K, Staruchova M, Grancicova E, Klvanova J, Ondreicka R. Effect of intake of exogenous vitamin C, E, ${\beta}$-carotene on the antioxidative status in kidney of rats streptozotocin-induced diabetic. Nahrung 1995; 39: 257-261   DOI   ScienceOn
48 Jennings PE, Barnett AH. New approaches to the pathogenesis and treatment of diabetic microangiopathy. Diabetes Med 1988; 5: 1111-1117