Wax Gourd Reduces Adipose Depots and Fat Cell Sizes without Affecting the Levels of Leptin in Rats Fed Medium Fat

  • Kang Keum-Jee (Department of Food and Nutrition, DukSung Women's University)
  • Published : 2006.08.01

Abstract

The purpose of this study was to investigate the effects of wax gourd on body weight, body fat, triglyceride, leptin and fat cell size in rats fed medium fat Male Sprague Dawley rats were fed with experimental diet containing total dietary fat at 20% of calorie with wax gourd (WG) for 4 weeks. Forty rats were divided into 4 groups including control, 5, 10, and 15% (w/w) WG supplement The results showed that weight gain was significantly higher in 5% WG than that in control (p<0.05), but was not different among the WG supplement groups. Total fat weight was significantly decreased in both 10 and 15% WG supplement groups than that of control. The levels of triglycerides and free fatty acids in the plasma were significantly decreased, particularly in 15% WG supplement group (p<0.05). The levels of leptin was not significantly different among the experimental groups. Fat cell size was significantly decreased in WG supplement groups compared to that of control group (p<0.05). Correlation among the parameters demonstrated that weight gain correlated positively with total fat weight, the levels of leptin and triglycerides. Taken these results together, 15% WG supplement diet substantially reduces total fat weight, triglycerides and free fatty acids levels and fat cell size in the rat fed midium fat.

Keywords

References

  1. Grundy SM. Multi-factorial of obesity: implications for prevention. Am J Clin Nutr 67(suppl):563s-572s, 1998
  2. Bray GA. Complications of obesity. Annals of Internal Medicine 103: 1052-1062, 1985 https://doi.org/10.7326/0003-4819-103-6-1052
  3. Huh KB. Cause of obesity. Korean J Nutr 23(5):333-336, 1990
  4. National Health and nutrition survey report. Ministry of Health and Welfare, 2001
  5. Albu J, Allison D, Boozer CN, Heymsfield S, Kissileff H, Kretser A, Krumhar K, Leibel R, Nonas C, Pi-Sunyer X, Vanltalline T, Wedral E. Obesity Solutions: report of a Meeting. Nutr Rev 55(5): 150-156, 1977
  6. Sisk M, Azin MJ, Hausman DB, Iewell DE. Effect of conjugated linoleic acid on fat pad weights and celluarity in Sprague Dawley and Zucker rats. FASEB 3116:A536, 1999
  7. Flier JS. The adipocyte:Storage depot or node on the energy information superhighway. Cell 80:15-18, 1995 https://doi.org/10.1016/0092-8674(95)90445-X
  8. Considine RV, Sinha MK, Heiman ML. Serum immunoreactiveleptin concentrations in normal weight and obese humans. N Engl J Med 334:292-295, 1996 https://doi.org/10.1056/NEJM199602013340503
  9. Behme MT. Leptin: Products of the obese gene. Nutrition Today 31(4):138-141, 1996 https://doi.org/10.1097/00017285-199607000-00002
  10. Mistry AM, Swick AG, Romos DR. Leptin rapidly lowers food intake and elevates metabolic rates in lean ob/ob mice. J Nutr 127:2065-2072, 1997
  11. Havel PJ. Role of adipose tissue in body-weight regulation: mechanism regulating leptin production and energy balance. Proc Nutr Soc 59(3):359-371, 2000
  12. Chan DCJ Watts GF, Barrett PH, Mamo JC, Redgrave TG. Markers of triglyceride rich lipoprotein remnant metabolism in visceral obesity. Clinical Chemistry Feb 48(2):278-283, 2002
  13. Elam MB, Wilcox Hg, Cagen LM, Deng X, Raghow R, Kumar P, Heimberg M, Russell JC. Increased hepatic VLDL secretion, lipoproteins and SREBP-1 expression in the corpulent JCRLACP rat. J Lipid Res 42(12):2039-2048, 2001
  14. Coppack SW, Jenson MD, Miles JM. In vivo regulation of lipolysis in humans. J Lipid Res 35:177-193, 1994
  15. Jenson MD, Haymond MW, Rizza RA, Cryer Pe, Miles JM. Influence of body fat distribution on free fatty acid metabolism in obesity. J Clin Invest 83(4):1168-1173, 1989 https://doi.org/10.1172/JCI113997
  16. Bruce R, Godsland I, Walton C, Crock D, Wynn V. Associations between insulin sensitivity and free fatty acid and triglyceride and triglyceride metabolism independent of uncomplicated obesity. Metabolism 43(10): 1275-1281, 1994 https://doi.org/10.1016/0026-0495(94)90222-4
  17. Song HS. The Encyclopedia of Crops in Korea. Pulkotnamoo, 1998
  18. Ahn DG. The Herb Illustrated-book of Korea. Kyohaksa, 1998
  19. Yoshizumi S, Murakami T, Kadoya M, Matsuda H, Yamahara J, Yoshikawa M. Medicinal food stiffs. XI. Histamine release inhibitors from wax gourd, the fruits of Benincasa hispida cogn. Cogn Yakugaku Zasshi 118:188-192, 2001
  20. Huh J. The Handbook of Oriental Medicine NamSanDang, 1994
  21. Lim SJ, Jeong JG, Kim MW, Choi SS, Han HK, Park JE. Effects of Benincasa hispida intake on blood glucose and lipid level on streptozotocin induced diabetic rats. Korean J Nutr 36(4):335-343, 2003
  22. Kang KJ, Lim SJ, Jeong JG, Han HK, Choi SS, Kim MH, K won SY. Effects of wax gourd on weight, triglyceride, leptin and fat cell size in rats fed on a high fat diet. Korean J Nutr 36(5):446-451, 2003
  23. Hong SS, Choi SY, Cha SK, Kim HJ, Park HJ, Lee YS. A study on the weight loss effect of wax gourd and the development of processed food from wax gourd. Korea Food Development Institute Report 10, 1999
  24. Korean Nutrition Society, Recommended Dietary Allowances for Koreans: 7th ed., 2000
  25. Kang KJ, Kim KH, park HS. Dietary conjugated linoleic acid did not affect on body fatness; fat cell sizes and leptin levels in male Sprague Dawley rats. Nutritional Sciences 5(3):117-122, 2002
  26. Park YH, Albright KJ, Liu W, Storkson JM, Cook ME, Pariza MW. Effect of conjugated linoleic acid on body composition in mice. Lipids 32(8):853-858, 1997 https://doi.org/10.1007/s11745-997-0109-x
  27. Despres JP. Abdominal obesity as important component of insulin resistant syndrom. Nutrition 19:452-459, 1993
  28. Jang JY, Choi HJ. Effects of Artenisia Iwayomogi oligosaccharide on the blood lipids, abdominal adipose tissues and leptin levels in the obese rats. J Korean Nutr 36(5):437-445, 2003
  29. Van Harmelen V, Reynisdottir S, Eriksson P, Thome A, Hoffstedt J, Lonnqvist F, Arner P. Leptin secretion from subcutaneous and visceral tissue in women. Diabetes 47 (6):913-917, 1998 https://doi.org/10.2337/diabetes.47.6.913
  30. Sjostrom L, William-olsson T. Prospective studies on adipose tissue development in man. Int J Obes 5:597-604, 1981
  31. Williams-roberts S, Worthtngton BS. Nutrition throught the life cycle. Mosby-year book: 7th ed., 2000
  32. Guo KY, Halo P, Leibel RL, Zhang Y. Effects of obesity on the relationship of leptir, mRNA expression and adipocyte size in anatomically distinct fat depots in mice. Am J Physiol Regul Integr Camp Physiol 287:R112-R119, 2004 https://doi.org/10.1152/ajpregu.00028.2004