• (The)Study of the Eastern Classic
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• no.41
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• pp.303-331
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• 2010

The theme, 'Good Life', is one of the most classical questions all over the oriental and European societies in the history of politic ideas. Confucius (BC. 551 ~ BC. 479), a philosopher representing oriental ideas, also sought 'Good World' by proposing new world, and such 'Good World' cannot be separated from 'Good Life', which is 'Happy Life', so that his foundation of Confucius School means that he dreamt of 'Confucianism based Happiness' in another words. This study is aimed to look into Confucius' viewpoint of happiness using Analects that best shows his ideas, words and behaviors. In the Chapter 2, I checked good life and happy life dealt in the ancient European societies to get a general cognition of them. Then, I looked into the details written in the Chapter 1 Introduction, Section 1 'HakEe(學而)', Analects, in which Confucius thought that the genuine happiness of human was the process toward internal joy from learning, external joy from communicating with society and humanistic human that was the completion of oneself, which I used as the keys for this study. In the Chapter 3, 4 and 5, therefore, I investigated how such keys shown in the Chapter 1 of the Section 'HakEe' were achieved all through Analects.

• Composites Research
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• v.34 no.4
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• pp.257-263
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• 2021

Composite materials have been widely applied for fabricating pressure vessels used for storing gaseous and liquid fuel because of their high specific stiffness and specific strength. Accordingly, the accurate measurement of their mechanical property, particularly the burst pressure or fracture strain, is essential prior to the commercial release. However, verification of the safety of composite pressure vessels using conventional test methods poses some limitations because it may lead to the deformation of the load transferring media or provoke an additional energy loss that cannot be ignored. Therefore, in this study, the segment-type ring burst test device was designed considering the theoretical load transferring ratio and applicable displacement of the vertical column. Moreover, to verifying the uniform distribution of pressure of the segment type ring burst test device, the hoop stress and strain distribution of ring specimens were compared with that of the hydraulic pressure test method via FEM. To conduct a simulation of the fracture behavior of the composite pressure vessel, a Hashin failure criterion was applied to the ring specimen. Furthermore, the fracture strain was also measured from the experiment and compared with that of the result from the FEM.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.1
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• pp.40-46
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• 2015

Arctic Ocean has rapidly melted due to global warming, by this, commercial ship has been operating through the area. Reason to develop the Northern Sea Route(NSR) even in extreme conditions, the distance than the existing route is shortened, which bring economic benefits. For these reason, the International Maritime Organization(IMO) established safety standards of the Arctic navigation(Polar Code) in order to ensure safe operation in the Northern Sea Route. In this study, it has been described ice types and safety standards of Artic vessel what officer needs to know for safe navigation on the Arctic Ocean. And It was verified by simulation the theoretical knowledge for the safe operation of the Arctic vessel. As a result, it was found that ship needs to reduce speed and analyze ice for safe operation before enter into the ice, it is necessary to enter at right angle to break ice safety and efficiently. Also according to the result of the simulation of navigation entering in ice channel(Lead), it was difficult to change course, it is believed that require emergency training for passing Vessel. In the future, It shall be analyzed precisely under various conditions of scenario.

• Journal of Advanced Marine Engineering and Technology
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• v.39 no.3
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• pp.312-317
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• 2015

Seafarers can confront to evacuate from the ship with many reasons such as collision, grounding and fire accident. It believes that evacuation time from ship is very important element in order to increase survival rate in the contingency circumstance, however narrow and complex structure of ship is one of obstacle element against prompt evacuation. Taking into consideration the unique structure of ship compared to the structure of other facilities, speed of fire propagation on board ship is faster than the same size of other type facilities. Therefore, measures to prompt evacuation are required. But it comes with the behavioral constraints of the crews and passengers of the nature of operating in a complex structure with narrow vessels. Therefore, in this study, we propose a formula to be analyzed by theoretical approach and simulation methods to improve the survival rate for the crew and passenger of the ship through the ship's structural modification. We analyzed the temperature rise and visibility which are the most influential effects on the life safety in the event of fire by using a three-dimensional analysis of sight-only program Fire Dynamic Simulator (FDS) as analytical tools.

• Molecules and Cells
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• v.40 no.12
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• pp.966-975
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• 2017

Excessive salt disrupts intracellular ion homeostasis and inhibits plant growth, which poses a serious threat to global food security. Plants have adapted various strategies to survive in unfavorable saline soil conditions. Here, we show that humic acid (HA) is a good soil amendment that can be used to help overcome salinity stress because it markedly reduces the adverse effects of salinity on Arabidopsis thaliana seedlings. To identify the molecular mechanisms of HA-induced salt stress tolerance in Arabidopsis, we examined possible roles of a sodium influx transporter HIGH-AFFINITY $K^+$ TRANSPORTER 1 (HKT1). Salt-induced root growth inhibition in HKT1 overexpressor transgenic plants (HKT1-OX) was rescued by application of HA, but not in wild-type and other plants. Moreover, salt-induced degradation of HKT1 protein was blocked by HA treatment. In addition, the application of HA to HKT1-OX seedlings led to increased distribution of $Na^+$ in roots up to the elongation zone and caused the reabsorption of $Na^+$ by xylem and parenchyma cells. Both the influx of the secondary messenger calcium and its cytosolic release appear to function in the destabilization of HKT1 protein under salt stress. Taken together, these results suggest that HA could be applied to the field to enhance plant growth and salt stress tolerance via post-transcriptional control of the HKT1 transporter gene under saline conditions.

• Journal of Advanced Marine Engineering and Technology
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• v.39 no.9
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• pp.923-928
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• 2015

The FDS is one of the most used programs for fire analysis and needs an optimal grid selection for an accurate analysis. This study selected various grid aspect ratios (ARs) for selection of optimal grid and analyzed them with FDS v 6.1.2. A calculation time of 10 min. was used, which is enough to obtain the time average value of temperature changes. Temperature, visibility, and the time average value of mass balance are obtained from 200-600 s, which is a period of maintaining quasi-steady state. Two polyurethane fires of 1 [MW] and 2 [MW] in two enclosures of $10{\times}10{\times}3[m^3]$ and $20{\times}20{\times}3[m^3]$ were considered. Time variations of heat release rates, temperature, visibility, and mass balance were compared for ARs from 1-6. The heat release rates were accurate for all aspect ratios regardless of fire and enclosure sizes. The quasi-steady state temperature and visibility were well predicted for $AR{\leq}5$. Temperature drop and skewness of mass conservation, however, increased with increasing aspect ratio. Therefore, careful investigation of the grid size is recommended in performance-based design when $AR{\geq}3$, where temperature and visibility in early stage of a fire are important parameters. For accurate simulations of enclosure fires, grid sizes of 0.1~0.2 [m] and smaller in the vertical direction and $AR{\leq}2$ are recommended.

• Molecules and Cells
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• v.23 no.2
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• pp.161-169
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• 2007

We identified two alternatively spliced variants of the peroxisomal targeting signal 1 (PTS1) receptor protein Pex5ps in monocot (rice, wheat, and barley) but not in dicot (Arabidopsis and tobacco) plants. We characterized the molecular and functional differences between the rice (Oryza sativa) Pex5 splicing variants OsPex5pL and OsPex5pS. There is only a single-copy of OsPEX5 in the rice genome and RT-PCR analysis points to alternative splicing of the transcripts. Putative light-responsive cis-elements were identified in the 5' region flanking OsPEX5L and Northern blot analysis demonstrated that this region affected light-dependent expression of OsPEX5 transcription. Using the pex5-deficient yeast mutant Scpex5, we showed that OsPex5pL and OsPex5pS are able to restore translocation of a model PTS1 protein (GFP-SKL) into peroxisomes. OsPex5pL and OsPex5pS formed homo-complexes via specific interaction domains, and interacted with each other and OsPex14p to form hetero-complexes. Although overexpression of OsPex5pL in the Arabidopsis pex5 mutant (Atpex5) rescued the mutant phenotype, overexpression of OsPex5pS only resulted in partial recovery.

• Journal of Life Science
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• v.20 no.1
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• pp.49-54
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• 2010

The purpose of this study is to examine the effect of Prunus mume supplementation on changes of energy substrate (glucose, FFA) and fatigue factors (lactate, ammonia, phosphorous) in the performance of exercise. The subjects of this study were 15 male university students. The exercise test was performed for 30 minutes at 75% $VO_2max$ on the treadmill and conducted both before and after administering Prunus mume for 6 weeks. Through Prunus mume supplementation, the accumulation of such fatigue factors as lactate, ammonia, and phosphorous along with concentration of glucuse decreased, but the concentration of FFA increased. From the study, it can be seen that Prunus mume plays a positive role for the use of energy substrates and accumulated fatigue factors.

• Journal of Life Science
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• v.23 no.4
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• pp.479-486
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• 2013

The DNA-binding protein from starved cells (DPS), originally identified as a DNA binding protein in Escherichia coli, is known to play an important role in DNA protection. The aim of this study was to evaluate the functional roles of DPS in E. coli against various kinds of external stresses by comparing the properties of wild-type E. coli cells and dps knockout mutant E. coli (${\Delta}dps$) cells. Under various stress conditions, we measured the cell growth of the wild-type E. coli and the dps knockout mutant E. coli (${\Delta}dps$) cells using a UV spectrophotometer. The growth rate of the cells was compared to investigate the functional roles of the DPS protein in E. coli. In comparison to the properties of the wild-type E. coli cells, the dps knockout mutant E. coli (${\Delta}dps$) cells showed highly sensitive phenotypes under various stress conditions, such as heat shock, acidic pH, nutrient deficiency, and different concentrations of reactive oxygen species (ROS), suggesting that DPS plays key roles in E. coli in combating diverse external stresses. The DPS DNA-binding protein in E. coli plays crucial roles in bacterial cell growth and in the protection of the cells from environmental stresses by tightly binding and preserving their DNA molecules.

• Korean Journal of Microbiology
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• v.32 no.2
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• pp.147-154
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• 1994

Nuclease was secreted to the environmental media from the Escherichia coli JM107 tranformant harboring the extracellular nuclease gene of Serratia marcescens in the plasmid of pNUC4. Under the growth conditions, the amount of secreted enzyme was increased in parallel with bacterial growth conditions, the amount of secreted enzyme was increased in parallel with bacterial growth. The enzyme was purified using chromatofraphic procedures of Matrex green gel and heparin agarose affinity gel, resulted in 50-fold purification with 15% recovery of the enzyme. The apparent molecular weight of the enzyme was estimated to be 29Kda by sodium dodecylsulfate denaturing gel electrophoresis. Using the purified enzyme, polyclonal antibody was obtained from the rabbit. The specificity of the antibody was confirmed by immunoblotting and immunoprecipitaion. For the investigation of cellular distribution of the enzyme, cells were fractionated into three fractions; cytoplasm, periplasm and extracellular fluid. While more than 80% of the enzymatic activity was detected in the extracellular fluid and periplasm, a little was found in the cytoplasm, indicating that the enzyme was likely to be immediately exported to the membrane for excretion after biosynthesis. These results were confirmed again by immunocytochemistry technique using the antibody.