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Molecular and Functional Characterization of Monocot-specific Pex5p Splicing Variants, Using OsPex5pL and OsPex5pS from Rice (Oryza sativa)  

Lee, Jung Ro (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Jung, Ji Hyun (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Kang, Jae Sook (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Kim, Jong Cheol (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Jung, In Jung (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Seok, Min Sook (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Kim, Ji Hye (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Kim, Woe Yeon (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Kim, Min Gab (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Kim, Jae-Yean (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Lim, Chae Oh (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Lee, Kyun Oh (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Lee, Sang Yeol (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Publication Information
Molecules and Cells / v.23, no.2, 2007 , pp. 161-169 More about this Journal
Abstract
We identified two alternatively spliced variants of the peroxisomal targeting signal 1 (PTS1) receptor protein Pex5ps in monocot (rice, wheat, and barley) but not in dicot (Arabidopsis and tobacco) plants. We characterized the molecular and functional differences between the rice (Oryza sativa) Pex5 splicing variants OsPex5pL and OsPex5pS. There is only a single-copy of OsPEX5 in the rice genome and RT-PCR analysis points to alternative splicing of the transcripts. Putative light-responsive cis-elements were identified in the 5' region flanking OsPEX5L and Northern blot analysis demonstrated that this region affected light-dependent expression of OsPEX5 transcription. Using the pex5-deficient yeast mutant Scpex5, we showed that OsPex5pL and OsPex5pS are able to restore translocation of a model PTS1 protein (GFP-SKL) into peroxisomes. OsPex5pL and OsPex5pS formed homo-complexes via specific interaction domains, and interacted with each other and OsPex14p to form hetero-complexes. Although overexpression of OsPex5pL in the Arabidopsis pex5 mutant (Atpex5) rescued the mutant phenotype, overexpression of OsPex5pS only resulted in partial recovery.
Keywords
${\beta}$-Oxidation; Alternatively Spliced Variants; Dicot; Light-dependent Expression; Monocot; OsPex5pL; OsPex5pS; Peroxisomal Targeting Signal 1 (PTS1);
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Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
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