• 동아시아식생활학회지
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• 제15권2호
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• pp.182-187
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• 2005

This study was performed to investigate the antimicrobial effect of the Viscum album var. coloratum extracts against food-borne pathogens. First, the Viscum album var. coloratum was extracted with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol, respectively. The antimicrobial activity of the Viscum album var. coloratum extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The petroleum ether extracts of Viscum album var. coloratum showed the highest antimicrobial activity against Bacillus cereus and Shigella dysenteriae. Synergistic effect in inhibition was observed when Viscum album var. coloratum extract was mixed with Perillae folium extract as compared with each extract alone. Finally, the growth inhibition curves were determined by using petroleum ether extracts of Viscum album var. coloratum against Bacillus cereus and Shigella dysenteriae. The petroleum ether extract of Viscum album var. coloratum had strong antimicrobial activity against Bacillus cereus at the concentration of 5,000 ppm. At this concentration, the growth of Bacillus cereus was retarded more than 24 hours and up to 12 hours for Shigella dysenteriae. In conclusion, the petroleum ether extracts of Viscum album var. coloratum inhibit efficiently Bacillus cereus and Shigella dysenteriae.

• Journal of Applied Biological Chemistry
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• 제65권1호
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• pp.75-79
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• 2022

겨우살이(Viscum album var. coloratum)의 80% 에탄올 추출물의 항산화, α-glucosidase 저해 및 항응고 효과를 확인하였다. 겨우살이 추출물의 수율은 30.9±0.4%이었고, 폴리페놀 함량은 57.6±1.5 mg GAE/g으로 나타났다. 겨우살이 추출물의 농도에 비례하여 유리라디칼 및 양이온라디칼 소거능, 아질산염 소거능, 환원력의 항산화 효과가 증가하였다. 또한 겨우살이 추출물은 α-glucosidase의 활성을 저해하였고, 혈액응고 체계의 외인성 경로와 공통 경로를 주로 저해함으로써 혈장 응고를 억제하였다.

• Natural Product Sciences
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• 제8권4호
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• pp.155-161
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• 2002

The mistletoe lectins are major active components in the extract of European mistletoe (Viscum album L) that have been widely used in adjuvant chemotherapy of cancer. This study was performed to investigate the antitumor activity of extract of Korean mistletoe (Viscum album var. coloratum) modified with Korean mistletoe lectin (Viscum album var. coloratum agglutinin, VCA). Compared with the results of VCA, survival rate was increased and experimental lung metastasis was reduced by treatment of modified extract (VCM). In addition, the treatment of VCM reduced angiogenesis and VCA-induced toxicity measured by a CAM assay. And VCM inhibited proliferation and induced apoptosis in vitro in tumor cells originated from tissues which are possible to apply topically without surgery. Taken together, the antitumor activities of VCM-treated group outperformed the activities of the VCA-treated group.

• Natural Product Sciences
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• 제8권4호
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• pp.152-154
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• 2002

The methanol extract of Viscum album var. coloratum, Korean mistletoe, showed potent prolongation effects on the bleeding time in rats in vivo, and whole blood clotting time and plasma recalcification time in rats ex vivo. The prolongation effect on the bleeding time of Korean mistletoe is comparable to that of Viscum album L., European mistletoe, 185.6% and 176.5%, respectively. However, the water extracts of the both plants did not show any prolongation effects. Platelet activating factor (PAF) receptor binding assay was carried out to elucidate the action mechanisms of the extracts, and both of the methanol extracts did not show any inhibitory activity. The $LD_{50}$ of the methanol extracts of both mistletoes are more than 2 g/kg. These results suggest that the mehtanol extract of Korean mistletoe might be a potential candidate to develop new drug to improve microcirculation.

• Applied Biological Chemistry
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• 제47권2호
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• pp.279-282
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• 2004

An antioxidant was isolated from Korean mistletoe (Viscum album var. coloratum) by consecutive purification using silica gel, Sephadex LH-20, and RP-HPLC. The active principle was identified as homo-flavoyadorinin-B (3',7-dimethoxyluteolin-4'-O-[apiosyl $(1{\rightarrow}2)$ glucoside]) by spectral analyses. It inhibited 74.6% of hydroxyl radical and 30.6% of superoxide anion radical at 0.01 mM; however, th~compound did not show any scavenging activity against hydrogen peroxide radical. At 0.1 mM, above compound scavenged superoxide anion radical about twice as effective as positive controls, BHT and ${\alpha}-tocopherol$. Radical scavenging activities of homo-flavoyadorinin-B on DPPH, hydroxyl, and hydrogen peroxide radicals were almost same with those of positive controls.

• 식물분류학회지
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• 제48권4호
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• pp.357-362
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• 2018

"향약채취월령"과 "동의보감"을 비롯한 우리나라 옛 문헌에 나오는 식물명 상기생(桑寄生)은 지금까지 뽕나무겨우살이(Loranthus parasiticus), 꼬리겨우살이(Loranthus tanakae), 참나무겨우살이(Taxillus yadoriki), 뽕나무겨우살이(Taxillus chinensis), 동백나무겨우살이(Korthalsella japonica), 그리고 겨우살이(Viscum album var. coloratum)로 알려져 왔다. 최근에는 상기생(桑寄生)이 국내에는 분포하지 않는다는 주장도 제기되었으나, 옛 문헌에 기록된 상기생은 중국에서 부르는 상기생, 즉 Taxillus sutchuenensis과는 다른 겨우살이(V. album var. coloratum)임이 확인되었다. 상기생을 뽕나무겨우살이라고 불렀으나, 최근에는 겨우살이로 부르고 있음이 확인되었다.

• 생약학회지
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• 제44권2호
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• pp.138-148
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• 2013

The three biochemical constituents pointed out as the bioactive principles of anticancer activity of Korean mistletoes, Viscum album var. colorantum, epiphyting to different host trees, different region, and different growth environments were compared. The characterizing compounds were lectin, which was glycoprotein reported has anticancer activity, homoflavoyadorinin-B, which was flavonoid glycoside reported as an antioxidant and oleanolic acid, as an anticancerous triterpenoid. We established the analysis methods for three characterizing compounds and determined by HPLC spectrophotometer, respectively. The average content of the lectin in V. album was 1.75 mg/g of dried plants and those of homoflavoyadorinin-B and oleanolic acid were 3.51 and 5.37 mg/g, respectively. The variations of contents of the three characterizing compounds according to epiphytic trees and growth region were observed but not found statistical significance clearly. We came to the conclusion that the contents of bioactive constituents of V. album except homoflavoyadorinin-B are not affected by epiphyting trees or growth environments.

• 대한화장품학회지
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• 제48권3호
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• pp.265-273
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• 2022

본 연구에서는 한국산 겨우살이 (Viscum album L. var. coloratum)이 아토피 피부염과 관련된 염증성 사이토카인에 영향을 미치는지 여부를 알아보았다. 실험에는 헥산, 부탄올, 에틸아세테이트, 메틸렌클로라이드, 총 4 가지 분획물을 사용하였으며, RAW264.7 마우스 대식세포와 RBL-2H3 렛트 호중구를 이용하여 염증성 마커를 연구하였다. 실험 결과 에틸아세테이트 분획이 tumor necrosis factor alpha (TNF-α), interleukin(IL)-6, IL-4의 mRNA 발현 및 단백질 분비량을 감소시켰으나, 헥산 분획은 뚜렷한 효능이 없었다. 또한 부탄올 분획은 IL-4, IL-6의 mRNA 발현을 감소시켰고, 메틸렌클로라이드 분획은 IL-4와 TNF-α의 mRNA 발현을 감소시켰다. 결과적으로 한국산 겨우살이(V. album var. coloratum)가 아토피 피부염과 관련된 사이토카인 분비를 억제시켜 항염증 효과를 나타낼 수 있으므로, 이와 관련된 기능성 화장품 개발이 가능할 것으로 사료된다.

• Journal of Forest and Environmental Science
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• 제29권2호
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• pp.173-180
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• 2013

Berries of Korean mistletoe (Viscum album var. coloratum [Kom.] Ohwi) contained one seed, which have, in general, one or two embryos but very rarely three embryos. Mucilaginous substances in berries may help them adhere to the branches of host trees. It was observed that seeds need more than one and half years to develop into normal and healthy seedlings from the time of inoculation. Many factors such as adhesion of berry, thickness of host branch, orientation of haustorial root, etc. influenced the successful development of mistletoe plants. Through the application of six-year observation results on the germination of seeds and growth of seedlings, about 80% of germination rate for mistletoe seeds and 61% of survival ratio for germinated seeds, which is more than 23 times higher in natural conditions, were obtained after inoculation of seeds on the one-year-old branches of Malus pumila var. dulcissima and Quercus mongolica trees. The technological aspects of the success can be applied to other host plants and provide a critical clue to an artificial propagation system, for this medicinally valuable genus. This is the first successful report on artificial inoculation and plant development of Korean mistletoe.

• 대한화장품학회지
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• 제48권4호
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• pp.295-302
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• 2022

본 연구에서는 한국산 겨우살이(Viscum album var. coloratum)의 항산화, 항노화, 미백 효능을 알아보았다. 겨우살이 분획물은 hexane (HX), ethyl acetate (EA), butanol (BU), methylene chloride (MC) 총 4 가지를 사용하였다. 실험 결과 HX 분획물이 4 가지 분획물 중 가장 높은 페놀 함량을 보였다. 또한 EA 분획물이 가장 높은 1,1-diphenyl-2-picrylhydrazyl (DPPH)자유 라디칼 소거능 및 catalase-유사 활성을 나타내었으며, MC 분획물이 가장 높은 superoxide dismutase (SOD)-유사 활성을 나타냈다. MC 분획물은 또한 가장 우수한 hyaluronidase와 elastase 억제능을 보였다. 미백 효능을 알아보기 위하여 tyrosinase 억제능을 알아보았는데, HX 분획물이 가장 우수한 억제능을 보여주었다. 본 연구 결과를 통하여 한국산 겨우살이는 항산화, 항노화 및 미백 활성을 가지는 기능성 화장품 소재로 사용이 가능할 것으로 보인다.