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Germination and Seedling Induction of Viscum album var. coloratum (Kom.) Ohwi after Artificial Inoculation on the Branch of Host Plants

겨우살이의 종자의 기주목 접종 및 유묘 활착기술

  • Kim, Chul-Woo (Department of Forestry, Graduate School, Kangwon National University) ;
  • Yi, Jae-Seon (Department of Forest Resources, College of Forest & Environmental Sciences, Kangwon National University)
  • 김철우 (강원대학교 대학원 임학과) ;
  • 이재선 (강원대학교 산림환경과학대학 산림자원학과)
  • Received : 2013.04.26
  • Accepted : 2013.05.15
  • Published : 2013.05.31

Abstract

Berries of Korean mistletoe (Viscum album var. coloratum [Kom.] Ohwi) contained one seed, which have, in general, one or two embryos but very rarely three embryos. Mucilaginous substances in berries may help them adhere to the branches of host trees. It was observed that seeds need more than one and half years to develop into normal and healthy seedlings from the time of inoculation. Many factors such as adhesion of berry, thickness of host branch, orientation of haustorial root, etc. influenced the successful development of mistletoe plants. Through the application of six-year observation results on the germination of seeds and growth of seedlings, about 80% of germination rate for mistletoe seeds and 61% of survival ratio for germinated seeds, which is more than 23 times higher in natural conditions, were obtained after inoculation of seeds on the one-year-old branches of Malus pumila var. dulcissima and Quercus mongolica trees. The technological aspects of the success can be applied to other host plants and provide a critical clue to an artificial propagation system, for this medicinally valuable genus. This is the first successful report on artificial inoculation and plant development of Korean mistletoe.

Keywords

References

  1. Bloksma N, Van Dijk H, Korst P, Willers JM. 1979. Cellular and humoral adjuvant activity of mistletoe extract. Immunobiol 156: 309-318.
  2. Bocci V. 1993. Mistletoe (Viscum album) lectins as cytokine inducers and immunoadjuvant in tumor therapy. A review. J Biol Regul Homeost Agents 7: 1-6.
  3. Choi SY, Chung SK, Kim SK, Yoo YC, Lee KB, Kim JB, Kim JY, Song KS. 2004. An antioxidant homo-flavoyadorinin-b from Korean Mistletoe (Viscum album var. coloratum). J Korean Soc Appl Biol Chem 47: 279-282.
  4. Choung BY. 1999. Study of Biological Activity components of korean and european mistletoe. MS thesis. Seoul Womens University, Korea.
  5. Doser C, Doser M, Hülsen H, Mechelke F. 1989. Influence of carbohydrates on the cytotoxicity of an aqueous mistletoe drug and of purified mistletoe lectins tested on human T-leukemia cells. Drug Res 39: 647-651.
  6. Franz H, Ziska P, Kindt A. 1981. Isolation and properties of three lectins from mistletoe (Viscum album L.). Biochem J 195: 481-484. https://doi.org/10.1042/bj1950481
  7. Hajto T, Hostanska K, Frei K, Rordorf C, Gabius HJ. 1990. Increased secretion of tumor necrosis factors alpha, interleukin 1, and interleukin 6 by human mononuclear cells exposed to beta- galactoside-specific lectin from clinically applied mistletoe extract. Cancer Res 50: 3322-3326.
  8. Hartmann HT, Kester DE, Davies FT Jr. 1990. Plant propagation: principles and practices (5th ed). Prentice-Hall, Inc, New Jersey, USA, pp 70-72.
  9. Jang CS, No KS. 2000. Anti-Diabetic Effect of Viscum album Lectin. Korean J Biomed Lab Sci 6: 151-157.
  10. Jeon YH. 2004. Study on the immunostimulant activity of the extract of korean mistletoe fruits to activate macrophages in mice. MS thesis. Konyang University, Korea.
  11. Jung JH. 2004. Adjuvant effect of korean mistletoe lectins on the induction of systemic and mucosal immunity by intranasal immunization. MS thesis. Handong Global University, Korea.
  12. Khwaja TA, Dias CB, Pentecost S. 1986. Recent studies on the anticancer activities of mistletoe (Viscum album) and its alkaloids. Oncol 43 Suppl 1:42-50. https://doi.org/10.1159/000226419
  13. Kim SW, Ko SM, Liu JR. 2008. In vitro Seed Germination and Callus Formation on Flower Bud of Korean Mistletoe (Viscum album L. var. coloratum [Kom.] Ohwi). Korean J Plant Biotech 35: 47-53. https://doi.org/10.5010/JPB.2008.35.1.047
  14. Korean National Arboretum. 2007. Korean Plant Names Index, p 47. ((in Korean)
  15. Kuttan G, Kuttan R. 1992. Immunomodulatory activity of a peptide isolated from Viscum album extract (NSC 635 089). Immunol Invest 21: 285-296. https://doi.org/10.3109/08820139209069368
  16. Lee BD. 2010. Host Specificity of Mistletoe (Viscum album var. coloratum [Kom.] Ohwi) and Its Artificial Cultivation. Ph.D thesis. University of Seoul, Korea.
  17. Lee GP. 2002. Identification and Purification of Lectin from Induced Callus in Korean Mistletoe (Viscum album var. coloratum). MS thesis. Dongguk University, Korea.
  18. Lee SJ. 2003. The screening of biological activities for korean viscum sp. classified by host trees. MS thesis. Kangwon Nationnal University, Korea.
  19. Park SM. 1999. Isolation and Characterization of Biological Activity High Molecular Substances from Viscum album var. coloratum. MS thesis. Seoul Womens University, Korea.
  20. Park CH, Park CG, Bang KH, Park HW, Seong NS. 2003. Plant Growth and Anatomical Characteristics of Korean Mistel (Viscum album var. coloratum [Kom.] Ohwi). Korean J Medicinal Crop Sci 11: 122-126.
  21. Peter J, Donald M, Robert O, Richard D. 1986. Characteristics of viscin from the seed of dwarf mistletoe. Bot Gaz 147: 156-158. https://doi.org/10.1086/337582
  22. Ribereau-Gayon G, Jung ML, Baudino S, Salle G, Beck JP. 1986. Effects of mistletoe (Viscum album L.) extracts on cultured tumor cells. Experientia 42: 594-599. https://doi.org/10.1007/BF01955552
  23. Schink M. 1997. Mistletoe therapy for human cancer: the role of the natural killer cells. Anticancer Drugs 8 Suppl 1:S47-S51. https://doi.org/10.1097/00001813-199704001-00011
  24. Seong K. 2004. Antiangiogenic mechanisms of Korean mistletoe lectin. MS thesis. Sungkyunkwan University, Korea.
  25. Seong NS. 2000. Technical developmentl for the utilization and cultivation of Korean mistletoe. Ministry of Agriculture and Forestry, Korea.
  26. Singh H, Johri BM. 1972. Development of gymnosperm seeds. In: Seed biology (Kozlowski TT, ed). Academic Press, New York, USA, pp 22-77.
  27. Visser J. 1981. South african parasitic flowering plants. Juta and Co, Cape Town, South Africa, pp 1-177.
  28. Yi JS, Kim CW. 2011. Apparatus for position fixing of mistletoe seeds. Korean Intellectual Property Office. Patent Number 10-1087173.
  29. Yoon TJ. 1997. Studies on the biological activities of lectin purified from Korean mistletoe (Viscum album var. coloratum) extracts and its characterization. Ph.D thesis. Konkuk University, Korea.