• 제목/요약/키워드: Unreported

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Unusual Applications of Kendrick Plots: Recalibration and Tolerance

  • Thierry N. J. Fouquet;Orlando Cabarcos
    • Mass Spectrometry Letters
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    • 제14권4호
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    • pp.173-177
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    • 2023
  • Kendrick plots offer an alternative visualization of mass spectral data which reveals ion series and patterning by turning a mass spectrum into a map, plotting the fractional mass (wrongly called mass defect) as a function of mass-to-charge ratios and ion abundances. Although routinely used for polymer mass spectrometry, two unreported applications of these Kendrick plots are proposed using the program "kendo2": the graphical recalibration of a mass spectrum via the simulation of a theoretical fractional mass and a multi-segment fit; and the rapid evaluation of scan-to-scan variation of accurate mass measurements used as tolerances for the blank subtraction of UPLC-MS data files. Both applications are compatible with any type of high-resolution MS data including LC/GC-MS(/MS).

Report on the Unrecorded Helvella Genus Found in Taebaek City, South Korea

  • Sangyoung Park;Sohee Kim;Eunjin Kim;Ju-Kyung Eo;Hwayong Lee
    • 한국균학회지
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    • 제51권4호
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    • pp.411-417
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    • 2023
  • In July 2023, previously unrecorded fungi belonging to the genus Helvella were collected from the city of Taebaek, South Korea. These fungi are morphologically similar to Helvella crispa, but their differences include a wide, saddle-shaped apothecium and white hair on the receptacle surface. By analyzing DNA sequences combining the internal transcribed spacer (ITS) and nuclear ribosomal large subunit (nrLSU) regions and comparing them with various related species of Helvella, the collected fungi were identified as H. orienticrispa.

A Korean case of CTCF related neurodevelopmental disorders

  • Seong Ryeong Kang;Soo Hyun Seo;Kyunghoon Kim;Hee Bum Yang;Hye Ran Yang;Anna Cho
    • Journal of Genetic Medicine
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    • 제20권2호
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    • pp.70-74
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    • 2023
  • CCCTC-binding factor (CTCF) is a transcriptional regulator that binds to a complex DNA motif in various orientations and plays a crucial role in regulating gene expression, chromatin restructuring, and developmental processes. Mutations in the CTCF are associated with neurodevelopmental disorders. Here we report the first Korean case with a de novo heterozygous variant in the CTCF (c.1025G>A; p.Arg342His). She showed global developmental delay, failure to thrive, and dysmorphic face, which are phenotypes consistent with previous reports in the autosomal dominant intellectual developmental disorder 21 (MIM 615502). She also showed clinical features not previously reported, such as antral web and tracheobronchomalacia. Our case follows suit and expands understanding of this rare disorder by reporting common features and, on the other hand, unreported concomitant congenital anomalies.

Report of two unrecorded yeast species in the class Tremellomycetes

  • Seonjae Kim;Sathiyaraj Srinivasan
    • Journal of Species Research
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    • 제13권2호
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    • pp.136-141
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    • 2024
  • The purpose of this study is to isolate and identify wild yeasts from the soil samples collected in Daegu and Daejeon City, Republic of Korea. Among 15 strains isolated in this study, 13 strains were previously reported and two strains had not been reported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains, PG2-2-10C and DJ2-14-10C, belong to the genus Holtermanniella (family Holtermanniaceae, order Holtermanniales, class Tremellomycetes) and Goffeauzyma (family Filobasidiaceae, order Filobasidiales, class Tremellomycetes), respectively. The two unrecorded yeast strains had oval shape and polar budding cells. This research describers the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

A report of six unrecorded bacterial species isolated from soil samples in Korea

  • Da Som Kim;Mi Jin Jeon;Won-Jae Chi
    • Journal of Species Research
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    • 제13권1호
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    • pp.61-66
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    • 2024
  • During an investigation of unrecorded prokaryotic species in Korea, six unrecorded bacterial strains were isolated from soil samples collected from Uljin-gun. Based on a similarity search using the 16S rRNA gene sequence of the isolated strains and the construction of the neighbor-joining phylogenetic tree, five strains were identified to the genus Pseudomonas of the family Pseudomonadaceae, while one strain was identified as a species belonging to the genus Paenibacillus of the family Paenibacillaceae. The details of these unreported species, including gram staining reaction, colony and cell morphology, basic biochemical characteristics, strain ID, and isolation source, are described in the description of the strains.

Isolation and characterization of two unrecorded yeast species in the phylum Basidiomycota

  • Jieun Seok;Jaewoo Bai;Sathiyaraj Srinivasan
    • Journal of Species Research
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    • 제13권1호
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    • pp.105-110
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    • 2024
  • The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Seoul and Daejeon, Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and carbon source assimilation test were done using API 20C AUX kit. Among the 13 isolated strains, 11 strains were previously reported, but two strains have never been reported from Republic of Korea. The 13 strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains B2UV-201 and DJ1-5-B-10C belong to the genera Rhodotorula and Rhodosporidiobolus, respectively. The two unrecorded yeast strains are oval shaped and polar budding cells. This research focuses on the morphological and biochemical properties of the two unreported yeast species that have not officially been reported in Korea.

APPLICATION OF FUZZY SET THEORY IN SAFEGUARDS

  • Fattah, A.;Nishiwaki, Y.
    • 한국지능시스템학회:학술대회논문집
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    • 한국퍼지및지능시스템학회 1993년도 Fifth International Fuzzy Systems Association World Congress 93
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    • pp.1051-1054
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    • 1993
  • The International Atomic Energy Agency's Statute in Article III.A.5 allows it“to establish and administer safeguards designed to ensure that special fissionable and other materials, services, equipment, facilities and information made available by the Agency or at its request or under its supervision or control are not used in such a way as to further any military purpose; and to apply safeguards, at the request of the parties, to any bilateral or multilateral arrangement, or at the request of a State, to any of that State's activities in the field of atomic energy”. Safeguards are essentially a technical means of verifying the fulfilment of political obligations undertaken by States and given a legal force in international agreements relating to the peaceful uses of nuclear energy. The main political objectives are: to assure the international community that States are complying with their non-proliferation and other peaceful undertakings; and to deter (a) the diversion of afeguarded nuclear materials to the production of nuclear explosives or for military purposes and (b) the misuse of safeguarded facilities with the aim of producing unsafeguarded nuclear material. It is clear that no international safeguards system can physically prevent diversion. The IAEA safeguards system is basically a verification measure designed to provide assurance in those cases in which diversion has not occurred. Verification is accomplished by two basic means: material accountancy and containment and surveillance measures. Nuclear material accountancy is the fundamental IAEA safeguards mechanism, while containment and surveillance serve as important complementary measures. Material accountancy refers to a collection of measurements and other determinations which enable the State and the Agency to maintain a current picture of the location and movement of nuclear material into and out of material balance areas, i. e. areas where all material entering or leaving is measurab e. A containment measure is one that is designed by taking advantage of structural characteristics, such as containers, tanks or pipes, etc. To establish the physical integrity of an area or item by preventing the undetected movement of nuclear material or equipment. Such measures involve the application of tamper-indicating or surveillance devices. Surveillance refers to both human and instrumental observation aimed at indicating the movement of nuclear material. The verification process consists of three over-lapping elements: (a) Provision by the State of information such as - design information describing nuclear installations; - accounting reports listing nuclear material inventories, receipts and shipments; - documents amplifying and clarifying reports, as applicable; - notification of international transfers of nuclear material. (b) Collection by the IAEA of information through inspection activities such as - verification of design information - examination of records and repo ts - measurement of nuclear material - examination of containment and surveillance measures - follow-up activities in case of unusual findings. (c) Evaluation of the information provided by the State and of that collected by inspectors to determine the completeness, accuracy and validity of the information provided by the State and to resolve any anomalies and discrepancies. To design an effective verification system, one must identify possible ways and means by which nuclear material could be diverted from peaceful uses, including means to conceal such diversions. These theoretical ways and means, which have become known as diversion strategies, are used as one of the basic inputs for the development of safeguards procedures, equipment and instrumentation. For analysis of implementation strategy purposes, it is assumed that non-compliance cannot be excluded a priori and that consequently there is a low but non-zero probability that a diversion could be attempted in all safeguards ituations. An important element of diversion strategies is the identification of various possible diversion paths; the amount, type and location of nuclear material involved, the physical route and conversion of the material that may take place, rate of removal and concealment methods, as appropriate. With regard to the physical route and conversion of nuclear material the following main categories may be considered: - unreported removal of nuclear material from an installation or during transit - unreported introduction of nuclear material into an installation - unreported transfer of nuclear material from one material balance area to another - unreported production of nuclear material, e. g. enrichment of uranium or production of plutonium - undeclared uses of the material within the installation. With respect to the amount of nuclear material that might be diverted in a given time (the diversion rate), the continuum between the following two limiting cases is cons dered: - one significant quantity or more in a short time, often known as abrupt diversion; and - one significant quantity or more per year, for example, by accumulation of smaller amounts each time to add up to a significant quantity over a period of one year, often called protracted diversion. Concealment methods may include: - restriction of access of inspectors - falsification of records, reports and other material balance areas - replacement of nuclear material, e. g. use of dummy objects - falsification of measurements or of their evaluation - interference with IAEA installed equipment.As a result of diversion and its concealment or other actions, anomalies will occur. All reasonable diversion routes, scenarios/strategies and concealment methods have to be taken into account in designing safeguards implementation strategies so as to provide sufficient opportunities for the IAEA to observe such anomalies. The safeguards approach for each facility will make a different use of these procedures, equipment and instrumentation according to the various diversion strategies which could be applicable to that facility and according to the detection and inspection goals which are applied. Postulated pathways sets of scenarios comprise those elements of diversion strategies which might be carried out at a facility or across a State's fuel cycle with declared or undeclared activities. All such factors, however, contain a degree of fuzziness that need a human judgment to make the ultimate conclusion that all material is being used for peaceful purposes. Safeguards has been traditionally based on verification of declared material and facilities using material accountancy as a fundamental measure. The strength of material accountancy is based on the fact that it allows to detect any diversion independent of the diversion route taken. Material accountancy detects a diversion after it actually happened and thus is powerless to physically prevent it and can only deter by the risk of early detection any contemplation by State authorities to carry out a diversion. Recently the IAEA has been faced with new challenges. To deal with these, various measures are being reconsidered to strengthen the safeguards system such as enhanced assessment of the completeness of the State's initial declaration of nuclear material and installations under its jurisdiction enhanced monitoring and analysis of open information and analysis of open information that may indicate inconsistencies with the State's safeguards obligations. Precise information vital for such enhanced assessments and analyses is normally not available or, if available, difficult and expensive collection of information would be necessary. Above all, realistic appraisal of truth needs sound human judgment.

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확률화응답모형(RRM)을 활용한 성매매조사 분석 (Analysis of Prostitution Survey Using Randomized Response Model(RRM))

  • 손창균;주재진
    • 한국콘텐츠학회논문지
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    • 제17권10호
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    • pp.65-71
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    • 2017
  • 통계조사 또는 실태조사는 '어떤 조사자가?', '어떤 목적으로?', 그리고 '어떻게?' 작성되었는가에 따라 왜곡의 가능성이 있는 것이 사실이다. 심지어는 통계결과는 '거짓말' 더 나아가서 범죄 또는 비행 등에 관한 통계는 '새빨간 거짓말'로 불리기도 한다. 범죄나 비행에 관한 통계를 신뢰하지 못하는 이유는 여러 가지를 들 수 있겠지만 그 중 대표적인 원인중의 하나가 숨은 범죄(Hidden Crime) 또는 형사사법기관에 보고되지 않는 범죄(Unreported Crime)가 존재한다는 것이다. 이러한 숨은 범죄문제를 보완하기 위해 피해자조사 또는 자기보고식 조사 등의 방법이 사용되고 있으나, 이 또한 범죄의 유형에 따라 과소보고 또는 과대보고의 문제가 있다. 범죄와 비행 그리고 일탈행동에 대한 조사는 매우 민감한 사항들이기 때문에, 조사대상자들은 심리적 부담을 가지게 된다. 이처럼 조사대상자들의 경험을 밝히는 것이 부담스러운 민감한 내용에 대해 진실한 답변을 유도할 수 있는 방법으로 통계학 분야에서 확률화응답모형(randomized response model)이 개발되어 사용되어왔다. 이 기법은 피해자조사 또는 자기보고식 조사의 문제점을 해결할 수 있는 매우 유용한 방법임에도 불구하고, 우리나라에서 범죄학 분야의 조사에서 사용된 경우는 매우 적다. 따라서 이 연구에서는 범죄학 분야연구에 있어 확률화응답모형의 적용가능성을 타진하기 위하여, 확률화응답모형을 활용하여 대학생들을 대상으로 성매매에 대한 내용을 실제로 측정해 보고, 확률화응답모형의 유용성을 확인해 보았다.

낙동강 하류 물금과 을숙도 수환경의 진핵 플랑크톤 종조성에 대한 분자모니터링 (Molecular Monitoring of Eukaryotic Plankton Diversity at Mulgeum and Eulsukdo in the Lower Reaches of the Nakdong River)

  • 이지은;이상래;윤석현;정상옥;이진애;정익교
    • 한국해양학회지:바다
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    • 제17권3호
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    • pp.160-180
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    • 2012
  • 본 연구는 메타게놈 분석법을 기초로 낙동강 하류 담수 환경의 물금과 기수 환경의 을숙도대교 정점에서 채수된 환경 시료내의 진핵 플랑크톤 종다양성 및 군집 구조를 비교 분석하고자 하였다. 수환경 시료에서 추출된 DNA에 대한 environmental Polymerase Chain Reaction(PCR)을 수행하여 18S rDNA 클론라이브러리를 구축하였고, colony PCR, PCR-Restriction Fragment Length Polymorphism(RFLP), 염기서열 결정 및 유사도 분석을 통하여 종다양성을 분석하였다. 물금 및 을숙도대교 정점에서 338개의 클론들을 분석하였고(170 clones, 물금; 168 clones, 을숙도대교), 그 결과 총 74개의 phylotype을 발굴하였다(49개, 물금; 25개, 을숙도대교). 발굴된 phylotype에 대한 계통 분석 결과, Stramenopiles, Cryptophyta, Viridiplantae, Alveolata, Rhizaria, Metazoa 및 Fungi 등의 분류군에 속하는 다양한 생물종이 발굴되었으며, 국내 미기록종 및 신종 후보 가능 생물종과 속(genus)이상의 새로운 분류학적 처리가 필요한 생물종의 존재를 확인하였다. 특히 Stramenopiles의 Pirsonia 및 Alveolata의 Perkinsea에 속하는 phylotypes 등 국내 미기록 생물종을 포함한 숨은 종다양성(cryptic species diversity)의 발굴은 분자모니터링 기법이 낙동강 하구역 수생태계 변화 모니터링을 위한 새로운 유용한 생물학적 정보를 제공할 수 있음을 제시하고 있다.

복숭아나무에서 검출된 Apricot pseudo-chlorotic leaf spot virus의 국내 첫 보고 (First Report of Apricot pseudo-chlorotic leaf spot virus Infecting Peach Trees in South Korea)

  • 박상민;서은철;김산영;박원흠;이수헌
    • 식물병연구
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    • 제23권1호
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    • pp.75-81
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    • 2017
  • 2016년 5월, 우리나라 복숭아의 주요산지인 경북 영천 지역에서 퇴록, 괴저반점, 엽맥퇴록, 황화와 같은 바이러스 병징과 이상증상을 보이는 복숭아 잎 24점을 채집하였다. 이 시료들의 바이러스 감염 여부를 확인하기 위해 RT-PCR 진단법을 이용하여 진단하였다. 진단 대상은 검역 병원체로 지정된 바이러스 또는 바이로이드를 중심으로, 국내외 연구를 참고하여 국내 발생가능성이 높거나 발생 시 위험도가 높은 종 17종이다. 진단결과, 국내에서 보고된 적이 없는 바이러스 1종(Apricot pseudo-chlorotic leaf spot virus, APCLSV)과 검역 바이로이드 1종(Peach latent mosaic viroid, PLMVd)을 포함하여, 총 7종의 바이러스와 바이로이드가 검출되었다. 바이러스를 동정하기 위해, RT-PCR 산물을 sequencing 하여 확인하였다. 또, 국내 미보고 종인 APCLSV가 검출된 시료를 이용하여 APCLSV의 외피단백질을 암호화하고 있는 염기서열을 증폭하여 결정하였다. 결정된 염기서열은 기 보고된 APCLSV 분리주와 97%의 상동성을 보였다. 이 외피단백질 염기서열을 Trichovirus속 바이러스들과 비교 분석하여, 최종적으로 APCLSV임을 확인하였다. 동정된 APCLSV를 Yeongcheon 분리주로 명명하고 결정된 외피단백질 염기서열은 NCBI GenBank에 등록하였다. APCLSV의 발생 양상을 보면, APCLSV가 검출된 시료들은 모두 Apple chlorotic leaf spot virus (ACLSV)가 복합감염되어 있었으며, APCLSV는 ACLSV와 유전학적 유연관계가 가까운 것으로 알려져 있다. ACLSV의 특성에 비추어 볼 때, APCLSV는 국내 과수 농가에 널리 퍼질 가능성이 높을 것으로 생각된다. 따라서, 추후 국내 농가에 미칠 영향 등에 관련된 연구가 필요하다.