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http://dx.doi.org/10.7850/jkso.2012.17.3.160

Molecular Monitoring of Eukaryotic Plankton Diversity at Mulgeum and Eulsukdo in the Lower Reaches of the Nakdong River  

Lee, Jee Eun (Division of Earth Environmental System, Pusan National University)
Lee, Sang-Rae (Marine Research Institute, Pusan National University)
Youn, Seok-Hyun (National Fisheries Research and Development Institute)
Chung, Sang Ok (Tidal Flat Research Institute, National Fisheries Research and Development Institute)
Lee, Jin Ae (School of Environmental Science and Engineering, Inje University)
Chung, Ik Kyo (Division of Earth Environmental System, Pusan National University)
Publication Information
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY / v.17, no.3, 2012 , pp. 160-180 More about this Journal
Abstract
We have studied the eukaryotic plankton species diversity to compare the community structure of fresh and brackish waters in the lower reaches of the Nakdong River using metagenomic methods. We constructed 18S rDNA clone libraries of total DNAs extracted from environmental water samples collected at Mulgeum (MG100929, fresh) and Eulsukdo bridge (ES, brackish). Through the steps of colony PCR, PCR-RFLP, sequencing and similarity analysis, we discovered the diverse species composition of eukaryotic plankton. Total 338 clones (170 at MG100929 and 168 at ES) were analyzed, and then we found 74 phylotypes (49 for MG100929 and 25 for ES). From the phylogenetic analysis, we confirmed various eukaryotic plankton of broad range of taxonomic groups, including Stramenopiles, Cryptophyta, Viridiplantae, Alveolata, Rhizaria, Metazoa, and Fungi. We also found several unreported species in Korea and candidates of new taxonomic entities at levels higher than genus. Especially, the cryptic species diversity including unreported phylotypes of Pirsonia (Stramenopiles) and Perkinsea (Alveolata) suggests that the molecular monitoring method can produce new informative biological data in monitoring the changes in the Nakdong River Mouth ecosystem.
Keywords
18S rDNA; Clone library; Metagenomics; Nakdong River; Eukaryotic plankton; Cryptic species diversity;
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