Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
권호 표지
DOI QR코드

DOI QR Code

Isolation and characterization of two unrecorded yeast species in the phylum Basidiomycota

  • Jieun Seok (College of Natural Science, Seoul Women's University) ;
  • Jaewoo Bai (College of Natural Science, Seoul Women's University) ;
  • Sathiyaraj Srinivasan (College of Natural Science, Seoul Women's University)
  • Received : 2023.11.15
  • Accepted : 2023.12.14
  • Published : 2024.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Seoul and Daejeon, Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and carbon source assimilation test were done using API 20C AUX kit. Among the 13 isolated strains, 11 strains were previously reported, but two strains have never been reported from Republic of Korea. The 13 strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains B2UV-201 and DJ1-5-B-10C belong to the genera Rhodotorula and Rhodosporidiobolus, respectively. The two unrecorded yeast strains are oval shaped and polar budding cells. This research focuses on the morphological and biochemical properties of the two unreported yeast species that have not officially been reported in Korea.

Keywords

Acknowledgement

This wotk was supported by a research grant from Seoul Women's University (2023-0159) and was also supported in part by National Research Foundation of Korea (NRF) funded by the MSIT(2022M3H9A1082984).

References

  1. Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25(17):3389-3402. https://doi.org/10.1093/nar/25.17.3389
  2. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791. https://doi.org/10.2307/2408678
  3. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  4. Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek, 73(4):331-371. https://doi.org/10.1023/A:1001761008817
  5. Masiulionis, V.E. and F.C. Pagnocca. 2017. Rhodosporid iobolus geoffroeae sp. nov., a basidiomycetous yeast isolated from the waste deposit of the attine ant Acromyrmex lundii. Int. J. Syst. Evol. Microbiol. 67:1028-1032. https://doi.org/10.1099/ijsem.0.001738
  6. Tamura, K., G. Stecher and S. Kumar. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol. Biol. Evol. 38:3022-3027. https://doi.org/10.1093/molbev/msab120
  7. Tiwari, S., A. Baghela and D. Libkind. 2021. Rhodotorula sampaioana fa, sp. nov., a novel red yeast of the order Sporidiobolales isolated from Argentina and India. Antonie van Leeuwenhoek, 114(8):1237-1244. https://doi.org/10.1007/s10482-021-01597-5
  8. Wang, Q.M., A.M. Yurkov, M. Goker, H.T. Lumbsch, S.D. Leavitt, M. Groenewald, ... and F.Y. Bai. 2015. Phylogenetic classification of yeasts and related taxa within Pucciniomycotina. Studies in Mycology 81(1):149-189. https://doi.org/10.1016/j.simyco.2015.12.002