• Journal of Species Research
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• 제13권2호
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• pp.136-141
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• 2024

The purpose of this study is to isolate and identify wild yeasts from the soil samples collected in Daegu and Daejeon City, Republic of Korea. Among 15 strains isolated in this study, 13 strains were previously reported and two strains had not been reported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains, PG2-2-10C and DJ2-14-10C, belong to the genus Holtermanniella (family Holtermanniaceae, order Holtermanniales, class Tremellomycetes) and Goffeauzyma (family Filobasidiaceae, order Filobasidiales, class Tremellomycetes), respectively. The two unrecorded yeast strains had oval shape and polar budding cells. This research describers the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

• Journal of Species Research
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• 제13권1호
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• pp.105-110
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• 2024

The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Seoul and Daejeon, Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and carbon source assimilation test were done using API 20C AUX kit. Among the 13 isolated strains, 11 strains were previously reported, but two strains have never been reported from Republic of Korea. The 13 strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains B2UV-201 and DJ1-5-B-10C belong to the genera Rhodotorula and Rhodosporidiobolus, respectively. The two unrecorded yeast strains are oval shaped and polar budding cells. This research focuses on the morphological and biochemical properties of the two unreported yeast species that have not officially been reported in Korea.

• Journal of Species Research
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• 제13권1호
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• pp.100-104
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• 2024

The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Daegu and Cheongju city, Republic of Korea. To identify the wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Among 13 strains, 11 strains were previously reported, but two strains were unreported from the Republic of Korea. The two unrecorded yeast strains, GW1-3 and PG1-1-10C, belong to the genus Solicoccozyma (family Piskurozymaceae, order Filobasidiales, class Tremellomycetes). The two strains had oval-shaped and polar budding cells. This research showed the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

• Journal of Species Research
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• 제9권3호
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• pp.198-203
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• 2020

The goal of this study was to isolate and identify wild yeasts from soil samples. The 15 wild yeast strains were isolated from the soil samples collected in Pocheon city, Gyeonggi Province, Korea. Among them, four yeast stains were unrecorded, and 11 yeast stains were previously recorded in Korea. To identify wild yeasts, microbiological characteristics were observed by API 20C AUX kit. Pairwise sequence comparisons of the D1/D2 domain of the 26S rRNA were performed using Basic Local Alignment Search Tool(BLAST). Cell morphology of yeast strains was examined by phase contrast microscope. All strains were oval-shaped and polar budding and positive for assimilation of glucose, 2-keto-ᴅ-gluconate, N-acetyl-ᴅ-glucosamine, ᴅ-maltose and ᴅ-saccharose (sucrose). There is no official report that describes these four yeast species: one strain of the genus Kodamaea in the family Metschnikowiaceae and three strains of the Hannaella in the family Bulleribasidiaceae. Kodamaea ohmeri YI7, Hannaella kunmingensis YP355, Hannaella luteola YP230 and Hannaella oryzae YP366 were recorded in Korea, for the first time.

• 한국균학회지
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• 제49권3호
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• pp.393-397
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• 2021

The unrecorded fungus Galactomyces pseudocandidus de Hoog & M.T. Sm. was isolated and cultured from an introduced species, Procambarus clarkii, in Korea. Two related species, Galactomyces citri-aurantii and Geotrichum candidum, have been reported, but no other Galactomyces spp. have been found in Korea. In this study, we isolated G. pseudocandidus from the digestive tract of American crayfish using RGY (river water-glucose-yeast) medium and then compared the morphological and molecular characteristics of this species with those of related species. As a result, we confirmed that G. pseudocandidus is an unrecorded fungus in Korea, so we described and reported the cultural characteristics and microstructures, such as conidia.

• Journal of Species Research
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• 제10권4호
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• pp.344-349
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• 2021

The purpose of this study was to isolate and identify wild yeasts from soil of Gyeongju city, and Haemadipsa rjukjuana of Gageodo Island, characterizing unrecorded yeast strains from Korea. The molecular analysis of the D1/D2 domain of 26S rDNA of yeast was performed using the Basic Local Alignment Search Tool (BLAST). No official report exists describing these three species: one species in the genus Candida, one species in the genus Debaryomyces, and one species in the genus Solicoccozyma. Candida saitoana YL9, Debaryomyces fabryi YL1, and Solicoccozyma terrea 20g9-1 are recorded for the first time from Korea. All three strains were oval shaped and polar binding, while positive for glucose, ᴅ-xylose, and ᴅ-cellobiose. Morphological, physiological, and biochemical properties are described in the species descriptions.

• Journal of Species Research
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• 제13권2호
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• pp.142-146
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• 2024

The purpose of this study was to isolate and identify wild yeasts from soil collected in Daegu City and Cheongyang County, Republic of Korea. Among 11 strains isolated in this study, nine strains were previously reported and two strains were unreported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Of the two unrecorded yeast strains, CY-9-10C belongs to the genus Mrakia (family Mrakiaceae, order Cystofilobasidiales, class Tremellomycetes) and PG3-4-10C belongs to the genus Slooffia (family Chrysozymaceae, order Microbotryomycetes incertae sedis, class Microbotryomycetes). Both strains had oval-shaped and polar budding cells. This research described the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

• Journal of Species Research
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• 제12권3호
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• pp.197-202
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• 2023

The purpose of this study was to isolate and identify wild yeasts from the soil collected in Gwangju and Pocheon City, Gyeonggi Province, Republic of Korea. Among 10 strains, six strains were already reported, but four strains were unrecorded in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were performed using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were carried out using API 20C AUX kit. The 10 strains were assigned to the phyla Basidiomycota (8 strains) and Ascomycota (2strains). The unrecorded four yeast strains, NH33, NH19, NH20, and YP416, belong to the phylum Basidiomycota and the genera Buckleyzyma, Leucosporidium, Holtermanniales, and Mrakia, respectively. All strains had oval-shaped and polar budding cells. In this research, the morphological and biochemical properties of four unreported yeast species were characterized intensively, which were not officially reported in Korea.

• Mycobiology
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• 제43권3호
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• pp.266-271
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• 2015

Several types of yeasts were isolated from wild flowers around Jangseong Lake in Jeollanam-do, Republic of Korea and identified by comparing the nucleotide sequences of the PCR amplicons for the D1/D2 variable domain of the 26S ribosomal DNA using Basic Local Alignment Search Tool (BLAST) analysis. In total, 60 strains from 18 species were isolated, and Pseudozyma spp. (27 strains), which included Pseudozyma rugulosa (7 strains) and Pseudozyma aphidis (6 strains), was dominant species. Among the 60 strains, Bullera coprosmaensis JS00600 represented a newly recorded yeast strain in Korea, and its microbiological characteristics were investigated. The yeast cell has an oval-shaped morphology measuring $1.4{\times}1.7{\mu}m$ in size. Bullera coprosmaensis JS00600 is an asporous yeast that exhibits no pseudomycelium formation. It grew well in vitamin-free medium as well as in yeast extract-malt extract broth and yeast extract-peptone-dextrose (YPD) broth, and it is halotolerant growing in 10% NaCl-containing YPD broth.

• 한국균학회지
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• 제45권1호
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• pp.23-30
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• 2017

각종 나뭇잎들의 효모 분포특성을 알아보기 위하여 대전광역시 서부지역에 위치한 옥녀봉과 연자산의 나뭇잎들로부터 야생효모들을 분리, 동정하였고 이들로부터 국내 미기록 효모들을 선별하여 균학적 특성을 조사하였다. 옥녀봉의 나뭇잎 20점으로부터 15종 22균주의 야생효모를 분리, 동정하였고 Cryptococcus bestiolae가 5주로 가장 많이 분리되었다. 또한 연자산 나뭇잎 20점에서는 모두 12종 24균주의 야생효모를 분리하였고 Aureobasidium pullulans가 8주로 가장 많았다. 옥녀봉과 연자산에서 분리한 야생효모 46균주 중에서 6종(Cryptococcus kuetzingii JSL508, Cryptococcus vishniacii JSL509, Dioszegia takashimae JSL510, Plowrightia periclymeni JSL514, Erythrobasidium hasegawianum JSL0193, Rhodotorula nothofagi JSL0196)을 국내에서 보고되지 않은 미기록 효모들로 최종 선별하였다. 이들의 균학적 특성을 조사한 결과 E. hasegawianum JSL0196은 자낭포자와 의균사를 형성하지 않았다. D. JSL510 외 모든 미기록 효모들은 내염성 또는 호염성이었고, C. JSL508과 D. JSL510은 $37^{\circ}C$에서 생육하는 고온성 효모들이었다.