Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Isolation and characterization of two unrecorded yeast species in the order Filobasidiales

  • Inyoung Choi (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Sathiyaraj Srinivasan (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2023.11.14
  • Accepted : 2023.12.14
  • Published : 2024.02.28
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Abstract

The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Daegu and Cheongju city, Republic of Korea. To identify the wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Among 13 strains, 11 strains were previously reported, but two strains were unreported from the Republic of Korea. The two unrecorded yeast strains, GW1-3 and PG1-1-10C, belong to the genus Solicoccozyma (family Piskurozymaceae, order Filobasidiales, class Tremellomycetes). The two strains had oval-shaped and polar budding cells. This research showed the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2021R1F1A1061389) and was also supported in part by National Research Foundation of Korea (NRF) funded by the MSIT(2022M3H9A1082984).

References

  1. Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25(17):3389-402. https://doi.org/10.1093/nar/25.17.3389
  2. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791. https://doi.org/10.2307/2408678
  3. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  4. Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4):331-371. https://doi.org/10.1023/A:1001761008817
  5. Li, A.H., Y. Zhou, B.S. Jia, Z.X. Liu, J.P. Sampaio and Y.G. Zhou. 2019. Heterocephalacria sinensis sp. nov., Phaeotremella lacus sp. nov. and Solicoccozyma aquatica sp. nov., three novel basidiomycetous yeast species isolated from crater lakes. International Journal of Systematic and Evolutionary Microbiology 69(12):3728-3739. https://doi.org/10.1099/ijsem.0.003637
  6. Liu, X.Z., Q.M. Wang, M. Goker, M. Groenewald, A.V. Kachalkin, H.T. Lumbsch, A.M. Millanes, M. Wedin, A.M. Yurkov, T. Boekhout and F.Y. Bai. 2015. Towards an integrated phylogenetic classification of the Tremellomycetes. Studies in Mycology 81(1):85-147. https://doi.org/10.1016/j.simyco.2015.12.001
  7. Tamura, K., G. Stecher and S. Kumar. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol. 38:3022-3027. https://doi.org/10.1093/molbev/msab120