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Galactomyces pseudocandidus (Dipodascaceae): An Unrecorded Yeast-like Fungus Isolated from the Digestive Tract of Procambarus clarkii

  • Eo, Ju-Kyeong (Bureau of Conservation & Assessment Research, National Institute of Ecology) ;
  • Park, Eunsu (Bureau of Conservation & Assessment Research, National Institute of Ecology) ;
  • Kim, Suhwan (Bureau of Survey & Safety Research, National Institute of Ecology) ;
  • Choe, Han-Na (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2021.09.08
  • Accepted : 2021.09.17
  • Published : 2021.09.30

Abstract

The unrecorded fungus Galactomyces pseudocandidus de Hoog & M.T. Sm. was isolated and cultured from an introduced species, Procambarus clarkii, in Korea. Two related species, Galactomyces citri-aurantii and Geotrichum candidum, have been reported, but no other Galactomyces spp. have been found in Korea. In this study, we isolated G. pseudocandidus from the digestive tract of American crayfish using RGY (river water-glucose-yeast) medium and then compared the morphological and molecular characteristics of this species with those of related species. As a result, we confirmed that G. pseudocandidus is an unrecorded fungus in Korea, so we described and reported the cultural characteristics and microstructures, such as conidia.

Keywords

Acknowledgement

This study was supported with funds from the National Institute of Ecology under project No. NIE-C-2021-19, NIE-A-2021-09 and the Ministry of Environment of Korea as a part of basic ecological research.

References

  1. Abu-Mejdad NMJA, Al-Badran AI, Al-Saadoon AH. New record of ascomycetous yeasts strains from soil in Basrah, Iraq. Drug Invent Today 2019;11:3073-80.
  2. Kurtzman C, Fell JW, Boekhout T. Galactomyces Redhead & Malloch (1977). The Yeasts: a taxonomic study. 5th edition ed. In: de Hoog GS, Smith MT, editors. London; New York: Elsevier; 2011. p. 413-20.
  3. Park JS. Fungus diseases of plants in Korea (1). The Bulletin of the College of Agriculture, Chungnam National University 1958;1:1-106.
  4. Lee S, Hong SB, Kim CY. Contribution to the checklist of soil-inhabiting fungi in Korea. Mycobiology 2003;31:9-18. https://doi.org/10.4489/MYCO.2003.31.1.009
  5. Park CW, Kim JW, Cho YJ, Kim JG, Lee MJ, Kim SH. Distribution of invasive alien species red swamp crawfish (Procambarus clarkii) in Korea. Korean J Ecol Environ 2020;53:331-5. https://doi.org/10.11614/KSL.2020.53.4.331
  6. Jones B, Buller N. Crayfish plague. Australian and New Zealand Standard Diagnostic Procedures 2000.
  7. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. San Diego: Academic Press, Inc.; 1990. p. 315-22.
  8. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  9. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547-9. https://doi.org/10.1093/molbev/msy096
  10. Munsell Color. The munsell book of color. Glossy collection. Grand rapids, Michigan: Munsell Color; 2012.
  11. de Hoog GS, Smith MT. Ribosomal gene phylogeny and species delimitation in Geotrichum and its teleomorphs. Stud Mycol 2004;50:489-515.