Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Description of unrecorded wild yeasts from soil in Republic of Korea under cold conditions

  • Soohyun Maeng (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Sathiyaraj Srinivasan (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2023.11.15
  • Accepted : 2023.12.14
  • Published : 2024.05.31
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Abstract

The purpose of this study was to isolate and identify wild yeasts from soil collected in Daegu City and Cheongyang County, Republic of Korea. Among 11 strains isolated in this study, nine strains were previously reported and two strains were unreported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Of the two unrecorded yeast strains, CY-9-10C belongs to the genus Mrakia (family Mrakiaceae, order Cystofilobasidiales, class Tremellomycetes) and PG3-4-10C belongs to the genus Slooffia (family Chrysozymaceae, order Microbotryomycetes incertae sedis, class Microbotryomycetes). Both strains had oval-shaped and polar budding cells. This research described the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (No.2021R1F1A1061389), also supported by National Institute of Biological Resources (NIBR) (2022M3H9A1082984) and a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202304104).

References

  1. Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25(17):3389-3402.
  2. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791.
  3. Kamura, K., G. Stecher and S. Kumar. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol. Biol. Evol. 38:3022-3027.
  4. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  5. Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73(4):331-371.
  6. Li, A.H., F.X. Yuan, M. Groenewald, K. Bensch, A.M. Yurkov, K. Li, P.J. Han, L.D. Guo, M.C. Aime, J.P. Sampaio, S. Jindamorakot, B. Turchetti, J. Inacio, B. Fungsin, Q.M. Wang and F.Y. Bai. 2020. Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: proposal of two new orders, three new families, eight new genera and one hundred and seven new species. Stud. Mycol. 96(1):17-140.
  7. Liu, X.Z. Q.M. Wang, M. Goker, M. Groenewald, A.V. Kachalkin, H.T. Lumbsch, A.M. Millanes, M. Wedin, A.M. Yurkov, T. Boekhout and F.Y. Bai. 2015. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud. Mycol. 81(1):85-147.
  8. Middelhoven, W.J. and F. Spaaij. 1997. Rhodotorula cresolica sp. nov., a cresol-assimilating yeast species isolated from soil. Int. J. Syst. Bacteriol. 47(2):324-327.
  9. Nakse, T., M. Suzuki and M. Itoh. 1987. Sporobolomyces yamatoanus, a new species of ballistosporous yeast equipped with ubiquinone-9 isolated from dead leaves of various plants in Japan. The Journal of General and Applied Microbiology 33(5):445-454.
  10. Park, Y., S. Maeng, J. Oh, G.H. Sung and S. Srinivasan. 2021. Mrakia terrae sp. nov. and Mrakia soli sp. nov., Two Novel Basidiomycetous Yeast Species Isolated from Soil in Korea. Mycobiology 49(5):469-475.
  11. Tsuji, M., Y. Tanabe, W.F. Vincent and M. Uchida. 2019. Mrakia hoshinonis sp. nov., a novel psychrophilic yeast isolated from a retreating glacier on Ellesmere Island in the Canadian High Arctic. Int. J. Syst. Evol. Microbiol. 69(4):944-948.
  12. Turchetti, B., C. Sannino, A. Mezzasoma, L. Zucconi, S. Onofri and P. Buzzini 2020. Mrakia stelviica sp. nov. and Mrakia montana sp. nov., two novel basidiomycetous yeast species isolated from cold environments. Int. J. Syst. Evol. Microbiol. 70(8):4704-4713.
  13. Wang, Q.M., A.M. Yurkov, M. Goker, H.T. Lumbsch, S.D. Leavitt, M. Groenewald, B. Theelen, X.Z. Liu, T. Boekhout and F.Y. Bai. 2015. Phylogenetic classification of yeasts and related taxa within Pucciniomycotina. Stud. Mycol. 81:149-189.
  14. Yurkov, A.M., C. Sannino and B. Turchetti. 2020. Mrakia fibulata sp. nov., a psychrotolerant yeast from temperate and cold habitats. Antonie Van Leeuwenhoek 113(4):499-510.