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Investigation of Microbial Communities in Sulculus diversicolor supertexta Through 16S rRNA Sequencing and Antibacterial Monitoring of Harmful Strains (16S rRNA 염기서열 분석을 통한 오분자기(Sulculus diversicolor supertexta)내 미생물 군집 조사 및 인체유해 질병세균에 대한 항균활성 모니터링)

  • Kim, Min-Seon;Lee, Seung-Jong;Heo, Moon-Soo
    • Journal of Life Science
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    • v.28 no.12
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    • pp.1477-1488
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    • 2018
  • This study investigated the muscles, intestines, and gonads of Sulculus diversicolor supertexta to examine the diversity of microbial communities within examples collected from the Jeju Coast. Using different media, initial pure isolation in MA, 1% BHIA, and 1% TSA indicated that the muscles, intestines, and gonads supported more communities, respectively. In analysis of relative similarity with 16s rRNA sequencing, 190 pure colonies were isolated, and further analysis with NBLAST identified 71 species, 39 genera, 25 families, and five phyla. Homogeny with the reference strain was 91-100%. Microbial communities in S. supertexta consisted of gamma and alpha Proteobacteria (48%), Actinobacteria (32.5%), Firmicutes (16.9%), Deinococcus-Thermus (1.3%), and Bacteroides (1.3%). In all tissue, Psychrobacter cibarius in Moraxellaceae was dominant. Alteromonadaceae, Enterobacteriaceae, Pasturellaceae, Moraxellaceae, Rhodobacteraceae, Geminicoccaceae, Dietziaceae, Intrasporangiaceae, Microbacteriaceae, Micrococcaceae, Micromonosporaceae, Streptomycetaceae, Aerococcaceae, Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylcoccaceae were commonly isolated across all tissues, and Flavobacteriaceae, Corynebacteriaceae, Yesiniaceae, Vibrionaceae, Hahellaceae, Pseudomonadaceae were also identified from the intestines. In microbial monitoring of four harmful bacteria, Streptomyces albus (96%) showed antibacterial activity against all four strains, and Agrococcus baldri (99%) and Psychrobacter nivimaris (99%) presented against E. Coli and E. aerogens. In addition, some strains with low homogeny were isolated and further experiments are therefore required, for example to refine the antimicrobial substances including new strain investigations. These additional experiments would aim to establish generic resources for the microbial communities in S. Supertexta and provide basic data for applied microbiological research.

Acoustic Doppler Current Profiler Bottom Tracking Survey of Flow Structures around Geumo Archipelago in the Southern Waters of Korea (ADCP bottom tracking에 의한 금오열도 주변의 해수유동)

  • Choo, Hyo-Sang
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.5
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    • pp.589-600
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    • 2019
  • In order to investigate the flow structures around Geumo archipelago on Southern Waters of Korea, water movements were measured for 25 hours during spring tide in May and neap tide in September 2002 using ADCP (Acoustic Doppler Current Profiler) attached to a running boat. Dominant directions of ebb and flood current at spring tide are SE-NW, representing the average flow rate of approximately 40cm/s in the surface layer. However because of the topographical reason, the direction and speed of the flow in the narrow waterway sea area around the northwest of Gae Island were different. There was no notable baroclinic component of tidal flow at spring tide. This indicates that the sea area has been actively engaged in vertical mixing due to island wake or eddy due to narrow waterways, shallow water depth and rapid flow rate around archipelago. At neap tide, dominant directions of tidal flows are SSE-NNW and the average flow rate in the surface layer is about 85 percent of the spring tide. The duration and intensity of the flow direction are shorter and less dominant than the spring tide. It is expected that asymmetrical tidal mixing will occur due to vertical velocity shear and horizontal eddies. From daily mean tidal flows obtained from the ADCP observation, it was found that the northwest of Gae Island have flows in NW~NE, the west of Geumo Island have the average currents of up to 21 cm/s WSW~SSW and counterclockwise circulation or eddy currents are formed in the west of Sori Island.

Multiple Genotypes of Avian Infectious Bronchitis Virus Circulating in Vietnam

  • Le, Tran Bac;Lee, Hyun-Jeong;Le, Van Phan;Choi, Kang-Seuk
    • Korean Journal of Poultry Science
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    • v.46 no.2
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    • pp.127-136
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    • 2019
  • Three strains of infectious bronchitis viruses (IBVs), designated VNUA3, VNUA8 and VNUA11, were isolated from diseased/infected chickens in Hanoi, Thainguyen, and Haiphong provinces of Vietnam. These birds had received a live IBV vaccination but still suffered from infectious bronchitis. VNUA3, VNUA8 and VNUA11 harbor cleavage sites (RRTGR, HRRRR, and HRRKR, respectively) within the S protein. A BLASTN search revealed that the S gene of VNUA3, VNUA8, and VNUA11 showed the highest nucleotide identity with those of IBV strains CK/Italy/I2022/13, CK/CH/LHLJ/08-6, and GX-NN120084, respectively. Phylogenetic analyses based on the S gene nucleotide sequences revealed that VNUA3, VNUA8 and VNUA11 clustered with Q1-like, QX-like and TC07-2-like genotypes, respectively, and were closely related to reference IBV strains from China. However, the Vietnam IBVs showed high divergence from vaccine strains 4/91 and Ma5, which are used in the Vietnamese farms from which the isolates were obtained. Taken together, these results indicate that at least three genotypes of IBV are circulating among chickens in North Vietnam. This is the first report of the molecular epidemiology of IBV in Vietnam.

Disaster Documentation through Oral History : Focus on Sinking of the MV Sewol (구술을 통한 재난 사고의 기록화 세월호 참사 관련 구술을 중심으로)

  • Song, Zoo Hyung
    • The Korean Journal of Archival Studies
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    • no.44
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    • pp.155-197
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    • 2015
  • Disaster of Sewol took place in one year. Meanwhile, the victim's family and the call time record academics have tried to record it. Ansan activists have moved to objectives such as civil records committee also pivotal. The citizens committee under the dictation recording oral history team has a diverse group of people associated with the time issue. bereaved families were collected from the oral as well as volunteers, religious personnel, activists, Ansan citizens and various people. Disasters around the world is also an important event to remember and honor the people together, and one of the most effective means to record There it is establishing an oral history archive. Does not leave a lot of nature history of sudden disasters that occur, as well as a tribute record dictation telling people of diverse perspectives on events helps a lot closer to the reality of the event. Erected in the National September 11 Memorial Museum to honor the Sept. 11 attacks and provide a variety of programs to chaerok dictation of the people involved with 9/11. To remember the 2013 Boston Marathon bombing 'Our Marathon' of crowdsourcing digital archive was built. In the archives of the local universities and institutions were created to collaborate actively and gathering oral history. Pan Am Flight 103 pieces terror has established an archive from Syracuse University. Here, neither graduates, faculty, and to the victim's family and friends gather and oral hitory. Disaster-related Sewol neither should be able to be used as in the case of foreign well, and it should continue to honor the victims of the collection. It also ought to occasion again to avoid this disaster on earth.

The Morpho-Climatic Characteristics of Stratified Slope Deposits in the Southwest Region of Haenam (해남 남서부지역의 Stratified Slope Deposit의 기후지형학적 특성)

  • PARK, Chul-Woong
    • Journal of The Geomorphological Association of Korea
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    • v.15 no.2
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    • pp.11-24
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    • 2008
  • Stratified slope was formed on the SSE-facing slope in the southwest region of Haenam, South Korea. Field and laboratory investigations into the geomorphology and sedimentology of stratified slope deposit that is inactive. Outcrops of this deposit show an alteration of coarse debris-supported matrix and tiny debris-supported matrix layers. Sedimentological analysis(particle-size analysis) indicates that this deposit is not fluvial process or only gravitation like rock-fall. Many clasts and fine materials on the slope is supposed to be product by congelifraction under Pleistocene periglacial climatic environment. Also The processes responsible for the genesis of this deposit probably are to move downward by gelifluction and to remove fine materials by slope wash in thawing cycle and in situ debris congelifraction on gelifluction slope. Now It is impossible to account for the time range of genesis(diurnal, seasonal). In conclusion, this stratified slope formed in cold and humid periglacial environmental in pleistocene, therefore, this slope is a periglacial relic landform, indicates that in south korea there was a cold and humid paleo-climate such as periglacial environmen.

Analysis of Chlorogenic Acid Content and Biological Activities of Aralia elata Ethanol Extract (두릅 에탄올 추출물의 Chlorogenic acid 함량 분석 및 생리활성)

  • Lee, Jeong Ho;Jeong, Kyoung Ok;Im, So Yeon;Jin, Da Mon;Lee, Wang Ro
    • Korean Journal of Plant Resources
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    • v.35 no.5
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    • pp.574-585
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    • 2022
  • This study was conducted to quantify chlorogenic acid content and evaluate biological activity, such as antioxidant, antibacterial, anti-inflammatory, and digestive enzyme activity of Aralia elata ethanol extract (AEE). The SC50 of DPPH and ABTS radical scavenging activities of AEE were 4.79±0.05 mg/mL, 5.79±0.05 mg/mL; total polyphenol and total flavonoid contents were 170.0±1.8 mgGAE/g, 105.5±4.1 mgQE/g, respectively. Nitric oxide (NO) was increased in RAW 264.7 cells and Caco-2 cells with treatment of LPS, and production of NO was inhibited by AEE in a concentration-dependent manner. Production of NO was reduced by 60.0±1.1% in RAW 264.7 cells and 50.7±2.8% in Caco-2 cells at of AEE. Similarly, the production of inflammatory cytokines (TNF-α, IL-1β and IL-6) was inhibited in a concentration dependent manner. Antibacterial activity increased as the dose concentration of AEE increased, and the MIC was 75 mg/mL for L. monocytogenes, and 100 mg/mL for S. typhimurium and H. pylori. In addition, amylase and protease enzyme activity was observed in AEE and increased enzyme activity was observed according to the concentration of the extract. AEE contained 7.06±0.01 mg/g of chlorogenic acid. As a result of the experiment, it is judged that it can be used as basic data for the development of health food using Aralia elata.

Changes in the work arrangements and new lifestyles after the COVID-19 pandemic: Evidence based on survey data from the Japanese Cabinet Office (코로나19 팬데믹 이후 일하는 방식의 변화와 새로운 라이프 스타일의 탐색 -코로나19 팬데믹 이후에 실시된 일본 내각부 조사자료를 중심으로-)

  • Lee, Sujin
    • Journal of Family Resource Management and Policy Review
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    • v.26 no.3
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    • pp.87-106
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    • 2022
  • This study compared working arrangements, interest in rural migration, and life satisfaction in Japan in two periods: immediately after the COVID-19 (2019 coronavirus disease) pandemic and two years after the global outbreak. The comparison was based on data from the "Survey on Changes in Attitudes and Behaviors in Daily Life under the Influence of Novel Coronavirus Infection, 2020, 2021", which was conducted four times by the Japanese Cabinet Office directly after the COVID crisis (May 2020 and September 2021). The respondents who participated in both the first and fourth surveys were employed individuals aged 20 years or older. The results are as follows. First, the proportion of Tokyo residents engaging in telework immediately after the COVID-19 pandemic was 36.1%, which is higher than the levels observed nationwide. Second, individuals involved in telework and those working under flexible arrangements were more highly interested in moving to rural areas than those who commute to work. Third, among people engaged in telework, life satisfaction diminished immediately after the COVID-19 pandemic compared with the period before this crisis. After two years of the pandemic, however, life satisfaction among this group improved. Changes in working arrangements due to the pandemic can be expected to promote migration, as well as help revitalize regions and encourage the discovery of new lifestyles.

Transparency Study of Descriptive Refueling and Signifying Chain Function - For the Efficiency of Media Language Education - (서술적 환유와 의미 연쇄 기능의 투명성 연구 -매체언어교육의 효율성을 위해-)

  • Lim, Ji-Won
    • Journal of Korea Entertainment Industry Association
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    • v.14 no.4
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    • pp.67-75
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    • 2020
  • Metonymy can be said to be the only language's meaning shifting technique that exists in the domain of a single human thought in order to obtain a transparent cognitive effect. The purpose of this study was to analyze the 'descriptive metonymy' of the advertising content language constructed by the cognitive principle and to find a way to use it in media language education for social and cultural interests and reflection of college students. The metonymy used in advertising media contrasts with the difficulty of the metaphorical interpretation of "opaque and distant" reasoning. Storyboards, mostly focused on human emotions and behaviors, used metonymy's 'transparent and easy meaning shifting technique'. I have found that I can expect the efficiency of media language education that contains the interest and sociocultural interest, self-reflection, and future imagination of college students. Now, there is less need to perform cognitive reasoning for advertisements with ambiguous metaphor techniques. Lastly, in order to produce successful advertising content, we expect to use the language technique of 'narrative metonymy' with warm feelings of humans, and acknowledge the lack of quantitative research and leave it as a task for the next research.

Celeactor as Cultural Contents : Focused on the Multi-Persona in a South Korean Reality Show Program (셀러엑터를 활용한 문화콘텐츠 : <놀면 뭐하니?>의 멀티 페르소나를 중심으로)

  • Han, Ae-Jin
    • Journal of Korea Entertainment Industry Association
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    • v.15 no.8
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    • pp.45-62
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    • 2021
  • This article examines celeactor as cultural contents focused on the multi-persona in a South Korean reality show program . The celeactor is a form of celebrity and part of a celetoid. Celebrity can be categorized by three forms: ascribed form, achieved form and attributed form. In attributed form, the celetoid suddenly becomes popular through the media. The celeactor is a subcategory of the celetoid. The celeactor is defined by a virtual character that exist in temporary or institutionalized traits of popular culture. The Korean celebrity culture presents Korean intellectual factors, spiritual aspects, tastes, moral virtue, power relationships and traditional hierarchy. In order to examine the features of the Korean celeactor in cultural contents, this article focuses on the multi-persona of celebrity in South Korean popular culture through fantasy, challenge and nostalgia. This article examines the multi-persona of celebrity represented in South Korean popular culture as a negotiated response to cultural identity deconstructed and reconstructed in performance. The research methodology is to analyze a South Korean television reality show program on MBC Hangout with Yoo that represents various sub-characters performed by Jae-suk Yoo, a South Korean comedian and host. As for theoretical underpinning, this exploration joins work on Erving Goffman's (1959) notion of self-presentation and Chris Rojek's (2001) celebrity studies.

Older Couples' Housework before and after Retirement (노년기 부부의 노동시장 진입과 탈퇴 이후 부인과 남편의 가사노동 변화)

  • Lee, Sujin
    • Journal of Family Resource Management and Policy Review
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    • v.26 no.1
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    • pp.43-58
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    • 2022
  • In this study, I analyzed how the hours and frequency of housework change after older couples' retire, using data from the 1st(2007) to 7th(2018) years of the Women's Family Panel (KLoWF) conducted at the Korea Women's Policy Research Institute. In this study, couples where both the wife and the husband were 65 years of age or older were selected for the survey. A total of 2,482 people participated. The results as follows. First, as comparing between two time points, when the weekday housework hours of the wife and husband at t1 time point increased, the weekday housework hours of the wife and husband at t2 time point also increased. Second, on weekdays, when the wife's housework increased, the husband's housework also increased. On the other hand, on weekend, when the wife's housework increased, then the husband's housework decreased on weekdays.