Browse > Article
http://dx.doi.org/10.5352/JLS.2018.28.12.1477

Investigation of Microbial Communities in Sulculus diversicolor supertexta Through 16S rRNA Sequencing and Antibacterial Monitoring of Harmful Strains  

Kim, Min-Seon (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Lee, Seung-Jong (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Heo, Moon-Soo (Major of Aquatic Life Medicine, Faculty of Marine Biomedical Sciences, Jeju National University)
Publication Information
Journal of Life Science / v.28, no.12, 2018 , pp. 1477-1488 More about this Journal
Abstract
This study investigated the muscles, intestines, and gonads of Sulculus diversicolor supertexta to examine the diversity of microbial communities within examples collected from the Jeju Coast. Using different media, initial pure isolation in MA, 1% BHIA, and 1% TSA indicated that the muscles, intestines, and gonads supported more communities, respectively. In analysis of relative similarity with 16s rRNA sequencing, 190 pure colonies were isolated, and further analysis with NBLAST identified 71 species, 39 genera, 25 families, and five phyla. Homogeny with the reference strain was 91-100%. Microbial communities in S. supertexta consisted of gamma and alpha Proteobacteria (48%), Actinobacteria (32.5%), Firmicutes (16.9%), Deinococcus-Thermus (1.3%), and Bacteroides (1.3%). In all tissue, Psychrobacter cibarius in Moraxellaceae was dominant. Alteromonadaceae, Enterobacteriaceae, Pasturellaceae, Moraxellaceae, Rhodobacteraceae, Geminicoccaceae, Dietziaceae, Intrasporangiaceae, Microbacteriaceae, Micrococcaceae, Micromonosporaceae, Streptomycetaceae, Aerococcaceae, Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylcoccaceae were commonly isolated across all tissues, and Flavobacteriaceae, Corynebacteriaceae, Yesiniaceae, Vibrionaceae, Hahellaceae, Pseudomonadaceae were also identified from the intestines. In microbial monitoring of four harmful bacteria, Streptomyces albus (96%) showed antibacterial activity against all four strains, and Agrococcus baldri (99%) and Psychrobacter nivimaris (99%) presented against E. Coli and E. aerogens. In addition, some strains with low homogeny were isolated and further experiments are therefore required, for example to refine the antimicrobial substances including new strain investigations. These additional experiments would aim to establish generic resources for the microbial communities in S. Supertexta and provide basic data for applied microbiological research.
Keywords
Marine bacteria; microbial communities; Sulculus diversicolor supertexta;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ian, E., Malko, D. B., Sekurova, O. N., Bredholt, H., Ruckert, C., Borisova, M. E., Albersmeier, A., Kalinowski, J., Gelfand, M. S. and Zotchev, S. B. 2014. Genomics of sponge-associated Streptomyces spp. Closely related to Streptomyces albus J1074: Insights into marine adaptation and secondary metabolte biosynthesis potential. PLos One 9, e96719.   DOI
2 Ivanova, E. P., Sawabe, T. M., Gorshkova, N. M., Svetashev, V. I., Mikhailov, V. V., Nicol, D. V. and Christen, R. 2001. Shewanella japonica sp. nov. Int. J. Syst. Evol. Microbiol. 51, 1027-1033.   DOI
3 Kang, K. P. 2011. Studies on the population changes and basic physiological characters due to climate change on small abalone (Haliotis diversicolor aquatili) in Jeju island. Ph.M. dissertation, Jeju University, Jeju, Korea.
4 Park, M. W., Kim, B. H., Son, M. H., Choi, J. S. and Lee, J. S. 2014. Reproductive cycle of the abalone, Haliotis discus hannai collected from Jindo of Korea. Kor. J. Malacol. 30, 243-248.   DOI
5 Penesyan, A., Tebben, J., Lee, M., Thomas, T. S., Kjelleberg, S. F., Hrder, T. and Egan, S. 2011. Identification of the antibacterial compound produced by the marine epiphytic bacterium Pseudovibrio sp. D323 and related sponge-associated bacteria. Mar. Drugs 9, 1391-1402.   DOI
6 Ringo, E., Sperstad, S., Kraugerud, O. F. and Krogdahl, A. 2008. Use of 16S rRNA gene sequencing analysis to characterize culturable intestinal bacteria in Atlantic salmon (Salmo salar) fed diets with cellulose or non-starch polysaccharides from soy. Aquac. Res. 39, 1087-1100.   DOI
7 Ringo, E., Sperstad, S., Myklebus, R., Refstie, S. and Krogdahl, A. 2006. Characterisation of the microbiota associated with intestine of Atlantic cod (Gadus morhua L.): The effect of fish meal, standard soybean meal and a bioprocessed soybean meal. Aquaculture 261, 829-841.   DOI
8 Romanenko, L. A., Tanaka, N., Frolova, G. M. and Mikhailov, V. V. 2009. Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan. Int. J. Syst. Evol. Microbiol. 59, 1480-1486.   DOI
9 Kim, A. R. and Kim, D. H. 2015. Diversity of cultured and uncultured bacteria in the gut of olive founder Paralichthys olivaceus, Kfas. 48, 447-453.
10 Khan, S. T., Takagi, M. and Shin-ya, K. 2010. Diversity, salt requirement, and antibiotic production of actinobacteria isolated from marine sponges. Actinomycettologica 24, 18-23.   DOI
11 Kim, D. H. 2010. Global warming effect on marine environments and measure practices against global warming. J. Kor. Soc. Mar. Envrion. Saf. 4, 421-425.
12 Kim, D. H., Brunt, J. and Austin, B. 2007. Microbial diversity of intestinal countents and mucus in rainbow trout (Oncorhynchus mykiss). J. Appl. Microbiol. 102, 1654-1664.   DOI
13 Kim, D. W., Baik, K. S., Kim, M. S., Jung, B. M., Shin, T. S., Chung, G. H., Rhee, M. S. and Seong, C. N. 2007. Shewnella haliotis sp. nov, isolated from the gut microflora of abalone, Haliotis disucus hannai. Int. J. Syst. Evol. Microbiol. 57, 2926-2931.   DOI
14 Weid, I. V., Marques, J. M., Cunha, C. D., Lippi, R. K., Santos, S. C. C. D., Rosado, A. S., Lins, U. and Seldin, L. C. 2007. Identification and biodegradation potential of a novel strain of Dietzia cinnamea isolated from a petroleum-contaminated tropical soil. Syst. Appl. Microbiol. 30, 331-339.   DOI
15 Shashidhar, R. and Bandekar, J. R. 2009. Deinococcus piscis sp. nov., a radiation-resistant bacterium isolated from a marine fish. Int. J. Syst. Evol. Microbiol. 59, 2714-2717.   DOI
16 Sheu, S. Y., Jiang, S. R., Chen, C. A., Wang J. T. and Chen, W. M. 2011. Paracoccus stylophorae sp. nov., isolated from the reef-building coral stylophora pistillata. Int. J. Syst. Evol. Microbiol. 61, 2221-2226.   DOI
17 Shi, L. Y., Liang, S., Luo, X., Ke, C. H. and Zhao, J. 2017. Microbial community of Pacific abalone (Haliotis discus hannai) juveniles during a disease outbreak in South China. Aquac. Res. 48, 1080-1088.   DOI
18 Kim, E. J., Fathoni, A., Jeong, G. T., Jeong, H. D., Nam, T. J., Kong, I. S. and Kim, J. K. 2013. Microbacterium oxydans, a novel alginate-and laminarin-degrading bacterium for the reutilization of brown-seaweed waste. J. Environ. Manage. 130, 153-159.   DOI
19 Kim, M. S., Lee, S. J. and Heo, M. S. 2018. Research on the diversity of intestinal microbial communities of Red tilefish (Branchiostegus japonicus) by 16S rRNA sequence analysis. J. Life Sci. 28, 361-368.
20 Tanaka, R. J., Ootsubo, M. S., Sawabe, T. M., Ezura, Y. and Tajima, K. 2004. Biodiversity and in situ abundance of gut microflora of abalon (Haliotis discus hannai) determined by culture-independent techniques. Aquaculture 241, 453-463.   DOI
21 Bulushi, I. M., Poole, S. E., Barlow, R., Deeth, H. C. and Dykes, G. A. 2010. Speciation of Gram-positive bacteria in fresh and ambient-stored sub-tropical marine fish. Int. J. Food. Microbiol. 138, 32-38.   DOI
22 An, J. Y., Zhu, W. J., Liu, Y., Zhang, X. M., Sun, L. J., Hong, P. Z., Wang, Y. L., Xu, C. H., Xu, D. F. and Liu, H. M. 2015. Pruification and Characterization of a novel bacteriocin CAMT2 produced by Bacillus amyloliquefaciens isolated from marine fish Epinephelus areolatus. Food Control 51, 278-282.   DOI
23 Birkbeck, T. H., Laidler, L. A., Grant, A. N. and Cox, D. I. 2002. Pasteuella skyensis sp. nov., isolated from Atlantic salmon (Salmo Salar L.). Int. J. Syst. Evol. Microbiol. 52, 699-704.
24 Brinkmann, C. M., Kearns, P. S., Illidge, E. E. and Kurtboke, D. I. 2017. Diversity and Bioactivity of Marine Bacteria Associated with the Sponges Candidaspongia flabellata and Rhopaloeides odorabile from the Great Barrier Reef in Australia. Mol. Divers. 9, 39.   DOI
25 Carmen, S., Marta, P., Alejandro, L., Pilar, R., Cristina, C., Paz, Z., Rogelio, F., Fernando, R. and Fernando, D. L. C. 2011. Antitumor actinopyranones producted by Streptomyces albus POR-04-15-153 isoalted from a marine sediment. J. Nat. Prod. 74, 1590-1596.   DOI
26 Cortes, L. S., Urdiain, M., Mora, R. M., Prieto, L. and Rossello, M. R. 2015. Prokaryotic microbiota in the digestive cavity of the jellyfish cotylorhiza tuberculata. Syst. Appl. Microbiol. 38, 494-500.   DOI
27 Lee, J. Y., Kim, P. S., Hyun, D. W., Kim, H. S., Shin, N. R., Yun, J. H., Kim, M. S., Whon, T. W. and Bae, J. W. 2017. Microbulbifer echini sp. nov, isolated from the gastrointestinal tract of purple sea urcin, Heliocidaris crassispina. Int. J. Syst. Evol. Microbiol. 67, 998-1004.   DOI
28 Kim, S. H., Lee, C. H., Song, Y. B., Kim, B. Y., Hyun, S. Y. and Lee, Y. D. 2012. Reproductive cycle of small abalone, Haliotis diversicolor aquatilis in Jeju coastal waters. J. Dev. Reprod. 16, 145-153.
29 Kim, S. R., Jung, S. J. and Oh, M. J. 2007. Bacterial flora and antibiotics resistance of intestinal bacteria in the wild and cultured black rockfish Sebastes inermis. J. Fish. Pathol. 20, 221-227.
30 Lee, J. N., Shin, N. R., Lee, H. W., Roh, S. W., Kim, M. S., Kim, Y. O. and Bae, J. W. 2012. Kistimonas scapharcae sp. nov., isolated from a deae ark clam (Scapharca broughtonii), and emended descripition of the genus Kistimonas. Int. J. Syst. Evol. Microbiol. 62, 2865-2869.   DOI
31 Lee, S. D. 2008. Agrococcus jejuensis sp. nov., isolated from dried seaweed. Int. J. Syst. Evol. Microbiol. 58, 2297-2300.   DOI
32 Li, J., Long, L. J., Yang, L. L., Xu, Y., Wang, F. Z., Li, Q. X., Zhang, S. and Li, W, J. 2012. Janibacter alkaliphilus sp. nov., isolated from coral Anthogorgia sp. Anton. Leeuw. Int. J. G. 102, 157-162.   DOI
33 Yoon, J. W. and Hirose, M. Y. 2014. Aureisphaera galaxeae gen. nov., sp. nov., a marine member of the family Flavobacteriaceae isolated from the hard coral Galaxea fascicularis. Anton. Leeuw. Int. J. G. 107, 1379-1386.
34 Wul, H. B., Chen, W., Wang, G. H., Dail, S. K., Zhou, D. Y., Zhao, H. Z., Guo, Y. T., Ouyang, Y. C. and Li, X. 2012. Culture-dependent diversity of Actinobacteria associated with seagrass (Thalassia hemprichii). Afr. J. Microbiol. Res. 6, 87-94.
35 Yi, H. N., Schumann, P., Sohn, K. H. and Chun, J. S. 2004. Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with L-ornithine and L-serine in the peptidoglycan. Int. J. Syst. Evol. Microbiol. 54, 1585-1589.   DOI
36 Yoon, J. H., Lee, C. H., Kang, S. J. and Oh, T. K. 2005. Psychrobacter celer sp. nov., isolated from sea water of the South Sea in Korea. Int. J. Syst. Evol. Microbiol. 55, 1885-1890.   DOI
37 Zhiyong, L., Ye, H., Yan, L., Yi, H., Liming, H. and Xiaoling, M. 2007. 16S rRNA clone library-based bacterial phylogenetic diversity associated with three South China sea sponges. World. J. Microbiol. Biotechnol. 23, 1265-1272.   DOI
38 Zeng, Y. X., Yu, Y., Li, H. R. and Luo, W. 2015. Psychrobacter fjordensis sp. nov., a Psychrotolerant bacterium isolated from an Arctic fjord in Svalbard. Anton. Leeuw. Int. J. G. 108, 1283-1292.   DOI
39 Choi, E. J., Kwon, H. C., Sohn, Y. C. and Yang, H. O. 2010. Kistimonas asteriae gen nov., a gammaproteobacterium isolated from Asterias amurensis. Int. J. Syst. Evol. Microbiol. 60, 938-943.   DOI
40 Liu, J. J., Zhang, X. Q., Pan, J., Sun, C., Zhang, Y., Li, C, Q., Zhu, X. F. and Wu, M. 2013. Aequorivita viscosa sp. nov., isolated from an intertidal zone, and emended descriptions of Aequorivita antarctica and Aequorivita Capsosiphonis. Int. J. Syst. Evol. Microbiol. 63, 3192-3196.   DOI
41 Doi, H., Chinen, A., Fukuda, H. R. and Usuda, Y. 2016. Vibrio algivorus sp. nov., an alginate-and agarose-assimilating bacterium isolated from the gut flora of turban shell marine snail. Int. J. Syst. Evol. Microbiol. 66, 3164-3169.   DOI
42 Fernanda, P. C., Agustina, U., Myriam, G., Michael, S. and Beatriz, C. 2015. Culturable diversity and antimicrobial activity of Actinobacteria from marine sediments in Valparaiso bay, Chile. Front. Microbiol. 6, 737.
43 Foster, G., Ross, H. M., Malnick, H., Willems, A., Garcia, P., Reid, R. J. and Collins, M. D. 1996. Actinobacillus delphinicola sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from sea mammals. Int. J. Syst. Bacteriol. 46, 648-652.   DOI
44 Guo, J. C. 2017. Metarcoding Analyses of Gut Microbiome Compositions in Red Abalone (Haliotis Rufescens, Swainson, 1822) Fed Different Macroalgal Diets. Ph.M. dissertation, Monterey bay University, California, USA.
45 Harris, J. M. 1993. The presence, Nature, and role of Gut Microflora in aquatic invertebrates: A synthesis. Microb. Ecol. 25, 195-231.
46 Mien, P. T. 2014. Community of soft coral Alcyonium digitatum associated bacteria and their antimicrobial activities. Ph.M. dissertation, Christian-Albrechts-Kiel University, Kiel, Germany.
47 Liu, Y., Xie, Q. Y., Hong, K., Li, L., Zhoa, Y. M., Tang, Y. L., An, J. Y., Zhu, P. P. and Xu, C. H. 2013. Paracoccus siganidrum sp. nov., isolated from fish gastrointestinal tract. Anton. Leeuw. Int. J. G. 103, 1133-1139.   DOI
48 Manachini, P. L. and Forthina, M. G. 1998. Producion in sea-water of thermostable alkaline proteases by a halotolerant strain of Bacillus licheniformis. Biotechnol. Lett. 6, 565-568.
49 Marta, M. S., Dagmar, F., Ana, B., Cathrin, S., Brain, J. T., Peter, S., Reiner, M. K., Pilar, D. and Pastor, F. I. J. 2005. Paenibacillus barchinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the Ebro River delta. Int. J. Syst. Evol. Microbiol. 55, 935-939.   DOI
50 Mojtaba, M., Hamed, N., Javad, H. and Aboulghasem, R. 2013. Screening of antibacterial producing actinomycetes from sediments of the Caspian Sea. Int. J. Mol. Cell. Med. 2, 64-71.
51 Nishijima, M. K., Takadera, T. H., Imamura, N. T., Kasai, H., An, K. D., Adachi, K. K., Nagao, T. K., Sano, H. R. and Yamasato, K. H. 2009. Microbulbifer variabilis sp. nov. and Microbulbifer epialgicus sp. nov., isolated from Pacific marine algae, possess a rod-coccus cell cycle in association with the growth phase. Int. J. Syst. Evol. Microbiol. 59, 1696-1707.   DOI
52 Park, J. Y., Kim, W. S., Kim, H. Y. and Kim, E. H. 2016. Potential use of Bacillus amyloliquefaciens as a probiotic bacterium in abalone culture. J. Fish. pathol. 29, 35-43.   DOI
53 Huo, Y. Y., Xu, X. W., Li, X., Liu, C., Cui, H. L., Wang, C. S. and Wu, M. 2011. Rugeria marina sp. nov., isolated from Marine Sediment. Int. J. Syst. Evol. Microbiol. 61, 347-350.   DOI
54 Hirak, R. D., Neelam, M., Jaya, C., Supriya, K. and Surajit, D. 2013. Maine bacteria : Potential candidates for enhanced bioremediation. Appl. Microbiol. Biotechnol. 97, 561-571.   DOI
55 Huang, Z. B., Guo, F., Zhao, J., Li, W. D. and Ke, C. H. 2010. Molecular analysis of the intestinal bacterial flora in cage-cultured adult small abalone, Haliotis diversicolor. Aquac. Res. 41, 760-769.   DOI