• Title/Summary/Keyword: UAA

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Removal of Fluoride Using Thermally Treated Activated Alumina (고온 처리된 활성알루미나를 이용한 불소 제거)

  • Park, Seong-Jik;Kim, Jae-Hyeon;Lee, Chang-Gu;Park, Jeong-Ann;Choi, Nag-Choul;Kim, Song-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.10
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    • pp.986-993
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    • 2010
  • In this study, sorption characteristics of thermally treated activated alumina (AA) for fluoride were investigated. Sorption experiments have been conducted in equilibrium and kinetic batch conditions. Also, effects of solution pH and anions on fluoride removal have been observed. The properties of thermally treated ( $700^{\circ}C$) activated alumina (AA700) and untreated activated alumina (UAA) were compared using field-emission scanning electron microscope, energy-dispersive spectrometry, X-ray diffractometer (XRD) analysis, and Brunauer-Emmett-Teller (BET) analysis. From the experiments using AA thermally treated at different temperatures (100, 300, 500, $700^{\circ}C$), it was found that at high fluoride concentrations (50, 100, 200 mg/L) the sorption capacity of thermally treated AA increased with increasing thermal treatment temperature. At an initial fluoride concentration of 200 mg/L, the sorption capacity of AA700 was 3.67 times greater than that of UAA. The BET analysis showed that the specific surface area of UAA was about 2 times larger than that of AA700. The XRD analysis indicated that UAA was composed of both boehmite (AlOOH) and bayerite ($Al(OH)_3$) while AA700 was $Al_2O_3$. The reason that fluoride sorption capacity of AA700 increased despite of decrease in specific surface area compared to UAA could be attributed to the change of crystal structure. The kinetic sorption test showed that fluoride sorption to AA700 arrived at equilibrium after 24 h. The equilibrium test demonstrated that the maximum sorption capacity of AA700 was 5.70 mg/g. Additional batch experiments indicated that fluoride sorption to AA700 was the highest at pH 7, decreasing at both acidic and basic solution pHs. Also, fluoride sorption to AA700 decreased in the presence of anions such as phosphate, nitrate, and carbonate. This study demonstrated that thermal treatment of AA at high temperature could increase its sorption capacity for fluoride.

Site-Specific Labeling of Proteins Using Unnatural Amino Acids

  • Lee, Kyung Jin;Kang, Deokhee;Park, Hee-Sung
    • Molecules and Cells
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    • v.42 no.5
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    • pp.386-396
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    • 2019
  • Labeling of a protein with a specific dye or tag at defined positions is a critical step in tracing the subtle behavior of the protein and assessing its cellular function. Over the last decade, many strategies have been developed to achieve selective labeling of proteins in living cells. In particular, the site-specific unnatural amino acid (UAA) incorporation technique has gained increasing attention since it enables attachment of various organic probes to a specific position of a protein in a more precise way. In this review, we describe how the UAA incorporation technique has expanded our ability to achieve site-specific labeling and visualization of target proteins for functional analyses in live cells.

Obstacle Avoidance Method in the Chaotic Unmanned Aerial Vehicle (카오스 무인 비행체에서의 장애물 회피 방법)

  • Bae, Young-Chul;Kim, Yi-Gon;Kim, Chun-Suk
    • Journal of the Korean Institute of Intelligent Systems
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    • v.14 no.7
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    • pp.883-888
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    • 2004
  • In this paper, we propose a method to avoid obstacles that have unstable limit cycles in a chaos trajectory surface. We assume all obstacles in the chaos trajectory surface have a Van der Pol equation with an unstable limit cycle. When a chaos UAVs meet an obstacle in an Arnold equation, Chua's equation and hyper-chaos equation trajectory the obstacle reflects the UAV( Unmanned Aerial Vehicle).

Application of Nano-sized Amphiphilic Polyurethane Particles for The Remediation of PAH-Contaminated Soil:Sorption to Soil and PAH Solubilization

  • Park, Heon-Sik;Ahn, Ik-Sung;Kim, Ju-Young;Park, Sung-Yong;Shin, Young-Seob
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2002.09a
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    • pp.183-186
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    • 2002
  • Experiments in the batch soil/aqueous systems were conducted to compare the sorption onto soil and extraction efficiency of sorbed phenanthrene in the presence of the micelle-forming anionic surfactant(SDS) or the micelle-like amphiphilic nano-sized polyurethane particles. Micelle-like amphiphilic nano-sized polyurethane(APU) particles synthesized from amphiphilic urethane acrylate anionomers(UAA) could solubilize phenanthrene within their hydrophobic interiors as the same way that surfactants micelles did in aqueous phase.

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Three ORF-Containing Group I Introns in Chloroplast SSU of Caulerpa sertularioides (Ulvophyceae) and Their Evolutionary Implications

  • Lee, Jung-Ho;Manhart, James R.
    • ALGAE
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    • v.18 no.3
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    • pp.183-190
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    • 2003
  • Except for a group I intron in trnL-uaa occuring in eubacteria and plastids, group I introns are rarely documented in plastid genomes. Here, we report that a green alga, Caulerpa sertularioides, contains three group IA3 introns in the 16S gene (cpSSU), CS-cpSSU.i1, CS-cpSSU.i2 and CS-cpSSU.i3. Each intron has an open reading frame with LAGLIDADG motifs. CS-cpSSU.i1orf and CS-cpSSU.i3orf occur at Loop 6 in the intron secondary structure and CScpSSU. i2orf at Loop 8. CS-cpSSU.i1orf and CS-cpSSU.i2orf contain both LAGLI-DADG motifs but CS-cpSSU.i3orf has only one. CS-cpSSU.i1 and CS-cpSSU.i2 share the insetion sites and the ORFs at Loop 6 and 8 with CpSSU·1 and CpSSU·2 introns of Chlamydomonas pallidostigmatica (Chlorophyceae). In contrast, CS-cpSSU.i3, containing 28 copies of GAAATAT at Loop 6, is a novel intron found only in Caulerpa sertularioides. Possible scenarios of the evolution of the three introns and their possible use in systematic research are discussed.

Phylogenetic Analysis of Pines Based on Chloroplast trnT-trnL Intergenic Spacer DNA Sequences

  • Um, Yurry;Park, Won-Kyu;Jo, Nam-Su;Han, Sim-Hee;Lee, Yi
    • Journal of Forest and Environmental Science
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    • v.30 no.3
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    • pp.307-313
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    • 2014
  • This study was conducted to distinguish the pines that are too similar to differentiate using conventional methods. Pinus densiflora and Pinus sylvestris have similar anatomical structure. They both have window-like pits and dentate ray tracheids, so it is not easy to distinguish the plants. We tried to find molecular markers by comparing chloroplast DNA sequences to differentiate the pines growing in Korea. We used P. densiflora, P. densiflora for. multicaulis, P. sylvestris, P. rigida, P. rigitaeda, P. koraiensis, and P. bungeana for this study. We found that the non-coding intergenic region of trnT(UGU) and trnL(UAA) genes have differences among the species. We designed a primer set to amplify the region efficiently and compared the PCR product sequences using CLC Workbench programs to find the polymorphism. We could distinguish the species using the sequences of the amplified region and the sequences were reproducible from the pines collected in Korea.

Molecular Data Concerning Alloploid Character and the Origin of Chloroplast and Mitochondrial Genomes in the Liverwort Species Pellia borealis

  • Pacak, Andrezej
    • Journal of Plant Biotechnology
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    • v.2 no.2
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    • pp.101-108
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    • 2000
  • The liverwort Pellia borealis is a diploid, monoecious, allopolypliod species (n=18) that as it was postulated, originated after hybridization and duplication of chromosome sets of two cryptic species: Pellia epiphylta-species N (n=9) and Pellia epiphylla-species 5 (n=9). Our recent results have supported the allopolyploid origin of P.borealis. We have shown that the nuclear genome of P.borealis consists of two nuclear genomes: one derived from P.epiphylla-species N and the other from P.epiphylla-species 5. In this paper we show the origin of chloroplast and mitochondrial genomes in an allopolyploid species P.borealis. To our knowledge there is no information concerning the way of mitochondria and chloroplast inheritance in Brophyta. Using an allopolyploid species of p. borealis as a model species we have decided to look into chloroplast and mitochondrial genomes of P.borealis, P.epiphylla-species N and P.epiphylla-species S for nucleotide sequences that would allow us to differentiate between both cryptic species and to identify the origin of organelle genomes in the alloploid species. We have amplified and sequenced a chloroplast $tRNA^{Leu}$ gene (anticodon UAA) containing an intron that has shown to be highly variable in a nucleotide sequence and used for plant population genetics. Unfortunately these sequences were identical in all three liverwort species tested. The analysis of the nucleotide sequence of chloroplast, an intron containing $tRNA^{Gly}$ (anticodon UCC) genes, gave expected results: the intron nucleotide sequence was identical in the case of both P.borealis and P.epiphyllaspecies N, while the sequence obtained from P.epiphyllasperies S was different in several nucleotide positions. These results were confirmed by the nucleotide sequence of another chloroplast molecular marker the chloroplast, an intron-contaning $tRNA^{Lys}$ gene (anticodon UUU). We have also sequenced mitochondrial, an intron-containing $tRNA^{Ser}$ gene (anticodon GCU) in all three liverwort species. In this case we found that, as in the case of the chloroplast genome, P.borealis mitochondrial genome was inherited from P.epiphylla-species N. On the basis of our results we claim that both organelle genomes of P.borealis derived from P.epiphylla-species N.

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A Study of the Vacating of Arbitral Awards by Finding Harmony of Case Law with Statutory Law of the United States (미국의 중재판정 취소에 관한 연구: 판례법과 제정법의 조화를 중심으로)

  • Kim, Chin-Hyon;Chung, Yong-Kyun
    • Journal of Arbitration Studies
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    • v.22 no.2
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    • pp.125-157
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    • 2012
  • This study is to vindicate the vacation of arbitral awards in the United States. It focuses on the harmony of case law with statutory law of the United States. Until the early twentieth century, the American legal system, having adopted the English common law view, harbored a hostile attitude toward arbitration. The purpose of the Federal Arbitration Act (FAA) of the United States, enacted in 1925, was to eliminate the hostile attitude of courts toward arbitration. Congress is to enforce arbitration agreements into which parties have entered and to place arbitration agreements upon the same footing as other contracts. The structure of grounds for vacating arbitration awards has two layers. One is of vacating grounds with statutory origins, such as the FAA and the Uniform Arbitration Act, and the other, of vacating grounds originating from a nonstatutory, case law background. For a while, vacatur based on case law has coexisted with vacatur on statutory grounds for arbitration awards. After the Supreme Court decision in Hall Street Associates, L.L.C. v. Mattel, Inc., however, the justification of vacating based on case law has weakened. Post-Hall Street decisions of circuit courts show ways to deal with manifest disregard of the law. One of them is the harmonization of the case law grounds for vacating with the statutory grounds. It seems that the manifest-disregard-of-law and public-policy exceptions show a possibility of survival after Hall Street. However, other nonstatutory grounds for vacation of arbitration awards have no firm basis after Hall Street.

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