Browse > Article
http://dx.doi.org/10.4490/ALGAE.2003.18.3.183

Three ORF-Containing Group I Introns in Chloroplast SSU of Caulerpa sertularioides (Ulvophyceae) and Their Evolutionary Implications  

Lee, Jung-Ho
Manhart, James R.
Publication Information
ALGAE / v.18, no.3, 2003 , pp. 183-190 More about this Journal
Abstract
Except for a group I intron in trnL-uaa occuring in eubacteria and plastids, group I introns are rarely documented in plastid genomes. Here, we report that a green alga, Caulerpa sertularioides, contains three group IA3 introns in the 16S gene (cpSSU), CS-cpSSU.i1, CS-cpSSU.i2 and CS-cpSSU.i3. Each intron has an open reading frame with LAGLIDADG motifs. CS-cpSSU.i1orf and CS-cpSSU.i3orf occur at Loop 6 in the intron secondary structure and CScpSSU. i2orf at Loop 8. CS-cpSSU.i1orf and CS-cpSSU.i2orf contain both LAGLI-DADG motifs but CS-cpSSU.i3orf has only one. CS-cpSSU.i1 and CS-cpSSU.i2 share the insetion sites and the ORFs at Loop 6 and 8 with CpSSU·1 and CpSSU·2 introns of Chlamydomonas pallidostigmatica (Chlorophyceae). In contrast, CS-cpSSU.i3, containing 28 copies of GAAATAT at Loop 6, is a novel intron found only in Caulerpa sertularioides. Possible scenarios of the evolution of the three introns and their possible use in systematic research are discussed.
Keywords
16S; Caulerpa; cpSSU; group IA3 intron; LAGLI-DADG motif containing ORF; Ulvophyceae;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Bao Y. and Herrin D.L. 1993. Nucleotide sequence and secondary structure of the chloroplast group I intron Cr.psbA2: novel features of this self-splicing ribozyrne. Nucleic Acids Res. 21: 1667   DOI   ScienceOn
2 Besendahl A, Qiu Y.-L., Lee J., Palmer J.D. and Bhattacharya D. 2000. The cyanobacterial origin and vertical transmission of the plastid tRNA(Leu) group-I intron. Curro Genet. 37: 12-23   DOI   ScienceOn
3 Burke J.M., Belfort M., Cech T.R, Davies RW., Schweyen RJ., Shub D.A, Szostak J.W. and Tabak H.F. 1987. Structural conventions for group I introns. NucleicAcids Res. 15: 7217-7221   DOI   ScienceOn
4 Manhart J.R. Kelly, K. Dudock a.s. and Palmer J.D. 1989. Unusual characteristics of Codium fragile chloroplast DNA revealed by physical and gene mapping. Mol. Gen. Genet. 216: 417-21
5 Manhart J.R and Palmer J.D. 1990. The gain of two chloroplast tRNA introns marks the green algal ancestors of land plants. Nature 345: 500-505
6 Manhart J.R 1995. Chloroplast 16S tRNA sequences and phylogenetic relationships of fern allies and ferns. Am. Fern. J. 85: 182-192   DOI   ScienceOn
7 Mattox K.R and Stewart K.D. 1983. Classification of the green algae: A concept based on comparative cytology. In: Irvine D.E.G. and John D.M. (eds). Systematics of the Green Algae. Academic Press. London. pp. 29-72
8 Meusnier I., Valero M., Destombe C., Gode C., Desmarais E., Bonhomme F., Starn W. T. and Olsen, J. L. 2002. Polymerase chain reaction-single strand conformation polymorphism analyses of nuclear and chloroplast DNA provide evidence for recombination, multiple introduc tions and nascent speciation in the Caulerpa taxifolia complex. Mol. Ecol. 11: 2317-2325   DOI   ScienceOn
9 Nozaki H., Iakahara M., Nakazawa A., Kita Y., Yamada I., Iakano H., Kawano S. and Kato M. 2002. Evolution of rbcL group IA introns and intron open reading frames within the colonial Volvocales (Chlorophyceae). Mol. Phylogenet. Evol. 23: 326-338   DOI   ScienceOn
10 Ohta N., Matsuzaki M., Misumi O., Miyagishima S., Nozaki H., Ianaka K., Shin-i T., Kohara Y. and Kuroiwa I. 2003. Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae. DNA Res. 10: 67-77   DOI   ScienceOn
11 Ihompson A.J. and Herrin D.L. 1991. In vitro self-splicing reactions of the chloroplast group I intron Cr.LSU from Chlamydomonas reinhardtii and in vivo manipulation via gene-replacement. Nucleic Acids Res.19: 6611-6618   DOI   ScienceOn
12 Qiu Y.-L., Cho Y., COX J.e. and Palmer J.D. 1998. The gain of three mitochondrial introns identifies liverworts as the earliest land plants. Nature 394: 671-674   DOI   ScienceOn
13 Qiu Y.-L. and Lee J. 2000. Iransition to a land flora: a molecular phylogenetic perspective. J. Phycol. 36: 799-802   DOI   ScienceOn
14 Reith M.E. and Munholland J. 1995. Complete nucleotide sequence of the Porphyra purpurea chloroplast genome. PlantMol. Biol. Rep. 13: 333-335   DOI
15 Douglas S.E. and Penny S.L. 1999. The plastid genome of the cryptophyte alga, Guillardia theta: Complete sequence and conserved synteny groups confirm its common ancestry Lee and Manhart: Caulerpa 16S Introns 189 with red algae. J. Mol. Evol. 48: 236-244   DOI   ScienceOn
16 Swofford D.L. 2000. Phylogenetic analysis using parsimony, version 4.0 beta.: User's manual. Illinois Natural History Survey, Champaign, IL
17 Turrnel M., Boulanger J. and Lemieux e. 1989. Two group I introns with long internal open reading frames in the chloroplast psbA gene of Chlamydomonas moetousii. Nucleic Acids Res. 17: 3875-3887   DOI   ScienceOn
18 Turmel M., Gutell RR, Mercier J.P., Otis C. and Lemieuxe. 1993. Analysis of the chloroplast large subunit ribosomal RNA gene from 17 Chlamydomonas taxa. Ihree internal transcribed spacers and 12 group I intron insertion sites. /. Mol. BioI. 232: 446-467   DOI   ScienceOn
19 Turrnel M., Mercier J.P., Cote V., Otis C. and Lemieux C. 1995. The site-specific DNA endonuclease encoded by a group I intron in the Chlamydomonas pallidostigmatica chloroplast small subunit rRNA gene introduces a single-strand break at low concentrations of Mg2+. Nucleic Acids Res. 23: 2519-2525   DOI   ScienceOn
20 Ustinova I., Krienitz L. and Huss V.A.R 2001. Closteriopsis acicularis (G.M. Smith) Belcher et Swale is a fusiform alga closely related to Chlorella kessleri Fott et Novakova (Chlorophyta, Irebouxiophyceae). Eur. J. Phycol. 36: 341-351   DOI   ScienceOn
21 Herrin D.L., Bao Y., Thompson AJ. and Chen Y.F. 1991. Selfsplicing of the Chlamydomonas chloroplast psbA introns. Plant Cell3: 1095-1107
22 XU M.-Q., Kathe S.D., Goodrich-Blair H., Nierzwicki-Bauer S.A. and Shub D.A. 1990. Bacterial origin of a chloroplast intron: conserved self-splicing group I introns in Cyanobacteria. Science 250: 1566-1570   DOI
23 Durocher V., Gauthier A, Bellemare G. and Lemieux C. 1989. An optional group I intron between the chloroplast small subunit rRNA genes of Chlamydomonas moewusii and C. eugametos. Curro Genet. 15: 277-282   DOI   ScienceOn
24 Evrard J.-L., Kuntz M., Straus N.A and Wei! J.-H. 1988. A classI intron in a cyanelle tRNA gene from Cyanophora paradoxa: phylogenetic relationship between cyanelles and plant chloroplasts. Gene71: 115-122   DOI   ScienceOn
25 Genetic Computer Group 1991. Program Manual for the GCG Package, Version 7. Madison, Wisconsin
26 Gloeckner G., Rosenthal A and Valentin K. 2000. The structure and gene repertoire of an ancient red algal plastid genome. J. Mol. Evol, 51: 382-390
27 Kapoor M., Wakasugi T., Yoshinaga K. and Sugiura M. 1996. The chloroplast chlL gene of the green alga Chlorella vulgaris C-27 contains a self-splicing group I intron. Mol. Gen. Genet. 250: 655-664
28 Kuhsel M.G., Strickland R and Palmer J.D. 1990. An ancient group I intron shared by eubacteria and chloroplasts. Nature 250: 1570-1573
29 Lee J. and Manhart J.R. 2002a. Four embryophyte introns and psbB operon indicate Chlorokybus as a basal Streptophyte lineage. Algae17: 53-58   DOI   ScienceOn
30 Lee J. and Manhart J.R 2002b. The chloroplast rp/23 gene cluster of Spirogyra maxima (Charophyceae) shares many similarities with the angiosperm rp/23 Operon. Algae17: 59-68   DOI   ScienceOn
31 Lucas P., Otis C., Mercier J.P., Turmel M. and Lemieux C. 2001. Rapid evolution of the DNA-binding site in LAGLIDADG homing endonucleases. NucleicAcids Res. 29: 960-969   DOI   ScienceOn
32 Lehman R and Manhart J.R 1997. A preliminary comparison of restriction fragment patterns in the genus Caulerpa (Chlorophyta) and the unique structure of the chloroplast genome of Caulerpa sertularioides. J. Phycol. 33: 1055-1062   DOI   ScienceOn