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Effect of Thiol Compounds and Antioxidants on In Vitro Development and Intracellular Glutathione Concentrations of Bovine Embryos Derived from In Vitro Matured and In Vitro Fertilized II. Effect of Antioxidants with Somatic Cells on Development and Intracellular Glutathione Concentrations of Bovine IVM/IVF Embryos (Thiol 화합물과 항산화제 첨가배양이 소 체외수정란의 체외발육과 세포내 Glutathione 농도 변화에 미치는 효과 II. 항산화제 첨가와 체세포 공동배양이 소 체외수정란의 체외발육과 세포내 Glutathione 농도 변화에 미치는 영향)

  • 양부근;박동헌;우문수;정희태;박춘근;김종복;김정익
    • Korean Journal of Animal Reproduction
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    • v.21 no.4
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    • pp.345-353
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    • 1997
  • Antioxidants and antioxidants with somatic cell co-culture, bovine oviduct epithelial cells(BOEC) and buffalo rat liver cells(BRLC), were studied as a mean of increasing the development and intracellular glutathione(GSH) concnetrations of bovine embryos derived from in vitro matured(IVM) and in vitro fertilized(IVF) oocytes. Cell numbers and intracellular GSH concentrations of blastocysts were also counted. The developmental rate beyond morula stages in CRlaa containing taurine(2.5mM), superoxide dismutase(SOD, 600U) and catalase(250U) were 1%, 75.0%, 64.8% and 62.3%, respectively. The developmental rate in antioxidant groups was significantly higher than in control(P<0.05). The intracellular GSH concentrations of blastocysts cultured in 0, 2.5mM taurine, 600U SOD and 250U catalase were 33.8pM, 39.3pM, 42.3pM and 54.8pM, respectively. This result indicated that the developmental rates and intracellular GSH concentrations of catalase group was significantly higher than any other groups(P<0.05). The developmental capacity in CRlaa plus various antioxidants co-cultured with BOEC were 55.3%(control), 74.1%(2.5mM taurine), 66.7%(600U SOD) and 70.7%(250U catalase) and in CRlaa plus various antioxidants co-cultured with BRLC in control, 2.5mM taurine, 600U SOD and 250U catalase were 63.8%, 75.5%, 71.0% and 73.5%, respectveily, the intracellular GSH concentrations of blastocyst embryos co-cultured with BOEC and BRLC in CRlaa with 0.25mM taurine, 600U SOD and 250U catalase were 73.4pM and 64.4pM, 79.9pM and 67.5pM, 82.3pM and 71.7pM, and 83.0pM and 80.0pM, respectively. Cell numbers of blastocysts were not difference in all experimental groups. These studies indicate that andtioxidants and antioxidant with somatic cell co-culture can increase the proportion of embryo that developed into morula and blastocysts, and the intracellular GSH concentrations of blastocyst embryos.

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Uranium and Radon Concentrations in Groundwater near the Icheon Granite (이천 화강암지역 지하수의 우라늄과 라돈 함량 특성)

  • Cho, Byong-Wook;Choo, Chang-Oh;Kim, Moon-Su;Lee, Young-Joon;Yun, Uk;Lee, Byeong-Dae
    • The Journal of Engineering Geology
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    • v.21 no.3
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    • pp.259-269
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    • 2011
  • Concentrations of uranium (U) and radon (Rn) were measured in groundwater from 74 wells in the Icheon area, with the aim of determining the range and distribution of concentrations in an area underlain by granite (in this case, the Icheon granite). U concentrations ranged from 0.02 to 1,640.0 ${\mu}g/L$ (median value, 2.03 ${\mu}g/L$) and Rn concentrations ranged from 40 to 23,400 pCi/L (median value, 4,649 pCi/L). U concentrations in 10.8% of the samples exceeded 30 ${\mu}g/L$, which is the maximum contaminant level (MCL) proposed by the US Environmental Protection agency (EPA), based on the chemical toxicity of U. In addition, U concentrations in 59.5% and 13.5% of the samples exceeded 4,000 pCi/L (the Alternative MCL (AMCL) of the US EPA) and 8,100 pCi/L (Finland’s guideline level), respectively. We found no significant correlations between U (Rn) and other constituents, except for U-$HCO_3$ (correlation coefficient of 0.71), U-Ca (0.69), U-Li (0.45), U-Sr (0.43), and U-F (0.42). U and Rn contents in the groundwater are low relative to those in areas in other countries with similar geological settings, possibly due to the inflow of shallow groundwater to the wells in the Icheon area.

Signed degree sequences in signed 3-partite graphs

  • Pirzada, S.;Dar, F.A.
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • v.11 no.2
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    • pp.9-14
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    • 2007
  • A signed 3-partite graph is a 3-partite graph in which each edge is assigned a positive or a negative sign. Let G(U, V, W) be a signed 3-partite graph with $U\;=\;\{u_1,\;u_2,\;{\cdots},\;u_p\},\;V\;=\;\{v_1,\;v_2,\;{\cdots},\;v_q\}\;and\;W\;=\;\{w_1,\;w_2,\;{\cdots},\;w_r\}$. Then, signed degree of $u_i(v_j\;and\;w_k)$ is $sdeg(u_i)\;=\;d_i\;=\;d^+_i\;-\;d^-_i,\;1\;{\leq}\;i\;{\leq}\;p\;(sdeg(v_j)\;=\;e_j\;=\;e^+_j\;-\;e^-_j,\;1\;{\leq}\;j\;{\leq}q$ and $sdeg(w_k)\;=\;f_k\;=\;f^+_k\;-\;f^-_k,\;1\;{\leq}\;k\;{\leq}\;r)$ where $d^+_i(e^+_j\;and\;f^+_k)$ is the number of positive edges incident with $u_i(v_j\;and\;w_k)$ and $d^-_i(e^-_j\;and\;f^-_k)$ is the number of negative edges incident with $u_i(v_j\;and\;w_k)$. The sequences ${\alpha}\;=\;[d_1,\;d_2,\;{\cdots},\;d_p],\;{\beta}\;=\;[e_1,\;e_2,\;{\cdots},\;e_q]$ and ${\gamma}\;=\;[f_1,\;f_2,\;{\cdots},\;f_r]$ are called the signed degree sequences of G(U, V, W). In this paper, we characterize the signed degree sequences of signed 3-partite graphs.

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Holdup and Flow Behavior of Fluidized Solid Particles in a Liquid-Solid Circulating Fluidized Bed

  • Lim, Dae Ho;Lim, Ho;Jin, Hae Ryong;Kang, Yong
    • Korean Chemical Engineering Research
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    • v.52 no.3
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    • pp.371-377
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    • 2014
  • Characteristics of holdup and flow behavior of fluidized solid particles were investigated in a liquid-solid circulating fluidized bed ($0.102m{\times}3.5m$). Effects of liquid velocity ($U_L$), particle size ($d_P$) and solid circulation rate ($G_S$) on the solid holdup, overall particle rising velocity, slip velocity between liquid and particles and hydrodynamic energy dissipation rate in the riser were examined. The particle holdup increased with increasing $d_P$ or $G_S$ but decreased with increasing $U_L$. The overall particle rising velocity increased with increasing $U_L$ or $G_S$ but decreased with increasing $d_P$. The slip velocity increased with increasing $U_L$ or $d_P$ but did not change considerably with $G_S$. The energy dissipation rate, which was found to be closely related to the contacting frequency of micro eddies, increased with increasing $d_P$, $G_S$ or $U_L$. The solid particle holdup was well correlated with operating variables such as $U_L$, $d_P$ and $G_S$.

A Study on Sexual Attitude, Autonomy and Harassment Experience of College Students (대학생의 성 태도, 성적 자율성 및 성희롱 경험에 관한 연구)

  • Kim, Soon-Gu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.3
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    • pp.223-231
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    • 2017
  • The purpose of this study was to determine the relationships among sexual attitudes, autonomy, and harassment experience and to find out how each factor affects the sexual attitudes of college students. The subjects were 236 college students in D city. The data were collected from September 3 - 8, in 2015 using a structured questionnaire. The data were analyzed using the SPSS WIN 18.0 program. For data analysis, a Mann-Whitney U-test, Kruskal-Wallis test, Pearson's correlation coefficients, and multiple regression analysis were carried out. The results are as follows. First, the average scores of sexual attitude, sexual autonomy, and sexual harassment experience were 4.01, 1.95, and 0.95 points, respectively. Second, the individual characteristics in sexual attitude were significantly different in gender (U=2124.50, p=0.000) and department (U=5741.00, p=0.026), sexual autonomy were considerably different in gender (U=2529.50, p=0.001) and satisfaction with personal relationships ($X^2=9.46$, p=0.009), and sexual harassment experience were significantly different in the department (U=5604.00, p=0.007). Third, a positive relationship was observed between the sexual attitudes and sexual autonomy (r=0.517, p<0.01). Sexual autonomy was found to influence the sexual attitudes (${\beta}=0.46$, p<.001). These results highlight the need to develop sex education programs in various situations for college students and that sex education should be implemented constantly.

Formulation of Fully Coupled THM Behavior in Unsaturated Soil (불포화지반에 대한 열-수리-역학 거동의 수식화)

  • Shin, Ho-Sung
    • Journal of the Korean Geotechnical Society
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    • v.27 no.3
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    • pp.75-83
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    • 2011
  • Emerging issues related with fully coupled Thermo-Hydro-Mechanical (THM) behavior of unsaturated soil demand the development of a numerical tool in diverse geo-mechanical and geo-environmental areas. This paper presents general governing equations for coupled THM processes in unsaturated porous media. Coupled partial differential equations are derived from three mass balances equations (solid, water, and air), energy balance equation, and force equilibrium equation. With Galerkin formulation and time integration of these governing equations, finite element code is developed to find nonlinear solution of four main variables (displacement-u, gas pressure-$P_g$), liquid pressure-$P_1$), and temperature-T) using Newton's iterative scheme. Three cases of numerical simulations are conducted and discussed: one-dimensional drainage experiments (u-$P_g-P_1$), thermal consolidation (u-$P_1$-T), and effect of pile on surrounding soil due to surface temperature variation (u-$P_1$-T).

INFINITELY MANY SMALL SOLUTIONS FOR THE p(x)-LAPLACIAN OPERATOR WITH CRITICAL GROWTH

  • Zhou, Chenxing;Liang, Sihua
    • Journal of applied mathematics & informatics
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    • v.32 no.1_2
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    • pp.137-152
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    • 2014
  • In this paper, we prove, in the spirit of [3, 12, 20, 22, 23], the existence of infinitely many small solutions to the following quasilinear elliptic equation $-{\Delta}_{p(x)}u+{\mid}u{\mid}^{p(x)-2}u={\mid}u{\mid}^{q(x)-2}u+{\lambda}f(x,u)$ in a smooth bounded domain ${\Omega}$ of ${\mathbb{R}}^N$. We also assume that $\{q(x)=p^*(x)\}{\neq}{\emptyset}$, where $p^*(x)$ = Np(x)/(N - p(x)) is the critical Sobolev exponent for variable exponents. The proof is based on a new version of the symmetric mountainpass lemma due to Kajikiya [22], and property of these solutions are also obtained.

Implementation of Agricultural Multi-UAV System with Distributed Swarm Control Algorithm into a Simulator (분산군집제어 알고리즘 기반 농업용 멀티 UAV 시스템의 시뮬레이터 구현)

  • Ju, Chanyoung;Park, Sungjun;Son, Hyoung Il
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2017.04a
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    • pp.37-38
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    • 2017
  • 최근 방제 및 예찰과 같은 농작업에 단일 UAV(Unmanned Aerial Vehicle)시스템이 적용되고 있지만, 가반하중과 체공시간 등 기존시스템의 문제가 점차 대두되면서 작업 시간을 보다 단축시키고 작업 효율을 극대화 할 수 있는 농업용 멀티 UAV시스템의 필요성이 증대되고 있다. 본 논문에서는 작업자가 다수의 농업용 UAV를 효과적으로 제어할 수 있는 분산군집제어 알고리즘을 제안하며 알고리즘 검증 및 평가를 위한 시뮬레이터를 소개한다. 분산군집제어는 UAV 제어 계층, VP(Virtual Point) 제어 계층, 원격제어 계층으로 이루어진 3계층 제어구조를 가진다. UAV 제어 계층에서 각 UAV는 point mass로 모델링 되는 VP의 이상적인 경로를 추종하도록 제어한다. VP 제어 계층에서 각 VP는 입력 $p_i(t)=u^c_i+u^o_i+u^{co}_i+u^h_i$-(1)을 받아 제어되는데 여기서, $u^c_i{\in}{\mathbb{R}}^3$는 VP 사이의 충돌방지제어, $u^o_i{\in}{\mathbb{R}}^3$는 장애물과의 충돌방지제어, $u^{co}_i{\in}{\mathbb{R}}^3$는 UAV 상호간의 협조제어, $u^h_i{\in}{\mathbb{R}}^3$는 작업자로부터의 원격제어명령이다. (1)의 제어입력에서 충돌방지제어는 각 $u^i_c:=-{\sum\limits_{j{\in}{\eta}_i}}{\frac {{\partial}{\phi}_{ij}^c({\parallel}p_i-p_j{\parallel})^T}{{\partial}p_i}}$-(2), $u^o_c:=-{\sum\limits_{r{\in}O_i}}{\frac {{\partial}{\phi}_{ir}^o({\parallel}p_i-p^o_r{\parallel})^T}{{\partial}p_i}}$-(3)로 정의되면 ${\phi}^c_{ij}$${\phi}^o_{ir}$는 포텐셜 함수를 나타낸다. 원격제어 계층에서 작업자는 햅틱 인터페이스를 통해 VP의 속도를 제어하게 된다. 이때 스케일변수 ${\lambda}$에 대하여 VP의 원격제어명령은 $u^t_i(t)={\lambda}q(t)$로 정의한다. UAV 시뮬레이터는 리눅스 환경에서 ROS(Robot Operating Systems)를 기반한 3차원 시뮬레이터인 Gazebo상에 구축하였으며, 마스터와 슬레이브 간의 제어 명령은 TCPROS를 통해 서로 주고받는다. UAV는 PX4 기반의 3DR Solo 모델을 사용하였으며 MAVROS를 통해 MAVLink 통신 프로토콜에 접속하여 UAV의 고도, 속도 및 가속도 등의 상태정보를 받을 수 있다. 현재 멀티 드론 시스템을 Gazebo 환경에 구축하였으며, 추후 시뮬레이터 상에 분산군집제어 알고리즘을 구현하여 검증 및 평가를 진행하고자 한다.

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EFFECT OF EXPERIMENTAL INFECll0N WITH STOMACH WORM (Haemonchus contortus) ON THE PRODUCTIVE PERFORMANCE OF DOES

  • Howlader, M.M.R.;Capitan, S.S.;Eduardo, S.L.;Sevilla, C.C.;Roxas, N.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.4
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    • pp.421-426
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    • 1996
  • Comparison of body weight changes of does infected with Haemonchus contortus (treatment groups 2 &3) and uninfected does (control group) was made using weekly body weight measurements over 35 weeks. The animals in treatment group 2 weighted significantly (p < 0.05) less than animals in the control group from the 5th week to the end of the experiment. Does in treatment group 3 had significantly (p < 0.05) lighter body weight than control does from the 13th week to the end of the study. The animals in control group gained an average of 0.75 kg. On the other hand, animals in infected groups 2 and 3 lost an average of 3.90 kg and 4.13 kg body weight, respectively. The animals in groups 2 and 3 also had significantly (p < 0.05) lower preslaughter and hot carcass weights than the controls.

Effect of Rumen Degradable Protein (RDP) in Straw Based Ration on Purine Derivatives Excretion and Microbial Nitrogen Supply in Cattle

  • Khandaker, Z.H.;Tareque, A.M.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.4
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    • pp.364-370
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    • 1997
  • Four local cattle were ($145{\pm}9.7kg$) used in a $4{\times}4$ Latin square design to study the effect of different levels of rumen degradable protein (RDP) in straw based ration on purine derivatives excretion and microbial N supply in cattle. The four rations were formulated at the same amount of energy but varying RDP approximately 50 (U0), 75 (U1), 100 (U2) and 150 (U3) percent levels of RDP requirement for maintenance. They were fed ranged from 101 to 304 g RDP/d. Apparent digestibility of all nutrients increased significantly (p < 0.01) in cattle fed ration U2 than other rations. Rumen $NH_3-N$ concentration increased from 43 to 130 mg/l in response of RDP intake. Purine derivatives excretion increased significantly (p < 0.01) with incremental level of 203 g RDP/d (U2) intake and positively correlated (r=0.69, p < 0.01, n=16) with amount of RDP intake. The rates of rumen microbial N supply were 16.8, 27.2, 39.1 and 32.9 g/d for rations U0, U1, U2 and U3 respectively. Efficiency of microbial N supply (EMNS) per kg of DOMR were 19.0, 25.3, 33.0, and 28.6 g and per MJ of ME. Intake were 0.62, 1.00, 1.44 and 1.21 g for U0, U1, U2 and U3 respectively and highest results were obtained in cattle fed U2 ration. Results of this study suggest that PD excretion and EMNS were increased as incremental level of RDP intake (U2) in local cattle.