• Title/Summary/Keyword: Two-step Method

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Comparative Analysis of Callus Induction and Plant Regeneration Rates Using One-step and Two-step Cultures for Rice Anther Cultivation (벼 약배양 1단계 및 2단계 배양을 이용한 캘러스 유도 및 식물 재부화율 비교 분석)

  • Park, Young-hie
    • Journal of Life Science
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    • v.31 no.4
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    • pp.385-388
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    • 2021
  • Anther cultivation for crop breeding is a method of rapid production of homozygosities by greatly reducing the time required for at least six generations to develop new varieties using conventional breeding methods. This technique of producing anther culture provides an opportunity to obtain more green plants from a methodological point of view, and the techniques that save time and effort in anther culture are also important because they increase the efficiency of culture. This study compared the callus induction rate and green plant regeneration rate of a one-step and a two-step culture that differ in their culture media and culture methods. One-step culture allows callus induction and plant regeneration in one medium, whereas two-step culture requires induction and plant regeneration in two different media. In this study, we compared the callus induction and plant regeneration rates of rice anthers as one-step and two-step cultures. The callus formation rate was 13.0% for one-step cultures and 8.6% for two-step cultures, so the rate was 4.4% higher for one-step cultures than for two-step cultures. The plant regeneration rate was 1.0% in one-step cultures and 3.0% in two-step cultures, so the regeneration rate was three times higher for the two-step cultures than for one-step cultures. This suggests that the two-step cultures are more efficient than the one-step cultures for haploid production.

Optimization of Multimodal Function Using An Enhanced Genetic Algorithm and Simplex Method (향상된 유전알고리듬과 Simplex method을 이용한 다봉성 함수의 최적화)

  • Kim, Young-Chan;Yang, Bo-Suk
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2000.11a
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    • pp.587-592
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    • 2000
  • The optimization method based on an enhanced genetic algorithms is proposed for multimodal function optimization in this paper. This method is consisted of two main steps. The first step is global search step using the genetic algorithm(GA) and function assurance criterion(FAC). The belonging of an population to initial solution group is decided according to the FAC. The second step is to decide the similarity between individuals, and to research the optimum solutions by simplex method in reconstructive search space. Two numerical examples are also presented in this paper to comparing with conventional methods.

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A Study on Dynamic Characteristics by Gearbox of High-speed Pump (고속 펌프용 증속기의 동특성에 관한 연구)

  • Lee, Dong-Hwan;Lee, Hyeong-U
    • 연구논문집
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    • s.31
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    • pp.65-75
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    • 2001
  • A dynamic model of pump having one step gearbox and two step gearbox is developed by the lumped parameter method. The model accounts for shafts, ball bearings and journal bearings flexibilities, gyroscopic effects and the force couplings among the transverse and torsion motions due to gearing. Excitation forces of pump having one step gearbox and two step gearbox are considered as the mass unbalance of the rotors and gear transmission error which comes from the modified tooth surface. A Campbell diagram, in which the excitation sources caused by the mass unbalance of the rotors and the transmitted errors of the gearing are considered, shows that there are no critical speeds at the operating speeds. One step and two step gearboxes are manufactured and are estimated for vibration/noise, lubrication and performance.

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A two-step approach for variable selection in linear regression with measurement error

  • Song, Jiyeon;Shin, Seung Jun
    • Communications for Statistical Applications and Methods
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    • v.26 no.1
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    • pp.47-55
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    • 2019
  • It is important to identify informative variables in high dimensional data analysis; however, it becomes a challenging task when covariates are contaminated by measurement error due to the bias induced by measurement error. In this article, we present a two-step approach for variable selection in the presence of measurement error. In the first step, we directly select important variables from the contaminated covariates as if there is no measurement error. We then apply, in the following step, orthogonal regression to obtain the unbiased estimates of regression coefficients identified in the previous step. In addition, we propose a modification of the two-step approach to further enhance the variable selection performance. Various simulation studies demonstrate the promising performance of the proposed method.

A fast damage detecting technique for indeterminate trusses

  • Naderi, Arash;Sohrabi, Mohammad Reza;Ghasemi, Mohammad Reza;Dizangian, Babak
    • Structural Engineering and Mechanics
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    • v.75 no.5
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    • pp.585-594
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    • 2020
  • Detecting the damage of indeterminate trusses is of major importance in the literature. This paper proposes a quick approach in this regard, utilizing a precise mathematical approach based on Finite Element Method. Different to a general two-step method defined in the literature essentially based on optimization approach, this method consists of three steps including Damage-Suspected Element Identification step, Imminent Damaged Element Identification step, and finally, Damage Severity Detection step and does not need any optimizing algorithm. The first step focuses on the identification of damage-suspected elements using an index based on modal residual force vector. In the second step, imminent damage elements are identified among the damage-suspected elements detected in the previous step using a specific technique. Ultimately, in the third step, a novel relation is derived to calculate the damage severity of each imminent damaged element. To show the efficiency and quick function of the proposed method, three examples including a 25-bar planar truss, a 31-bar planar truss, and a 52-bar space truss are studied; results of which indicate that the method is innovatively capable of suitably detecting, for indeterminate trusses, not only damaged elements but also their individual damage severity by carrying out solely one analysis.

Enhancement of Density and Piezoelectric Properties of 0.96(K0.456Na0.536)Nb0.95Sb0.05-0.04Bi0.5(Na0.82K0.18)0.5ZrO3 Lead-Free Piezoelectric Ceramics through Two-Step Sintering Method (Two-Step 소결법을 통한 0.96(K0.456Na0.536)Nb0.95Sb0.05-0.04Bi0.5(Na0.82K0.18)0.5ZrO3 무연 압전 세라믹의 밀도 및 압전 특성 향상)

  • Il-Ryeol Yoo;Sang-Hyun Park;Seong-Hui Choi;Kyung-Hoon Cho
    • Korean Journal of Materials Research
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    • v.34 no.2
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    • pp.116-124
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    • 2024
  • In this study, we investigated the microstructure and piezoelectric properties of 0.96(K0.456Na0.536)Nb0.95Sb0.05-0.04Bi0.5(Na0.82K0.18)0.5ZrO3 (KNNS-BNKZ) ceramics based on one-step and two-step sintering processes. One-step sintering led to significant abnormal grain (AG) growth at temperatures above 1,085 ℃. With increasing sintering temperature, piezoelectric and dielectric properties were enhanced, resulting in a high d33 = 506 pC/N for one-step specimen sintered at 1,100 ℃ (one-step 1,100 ℃ specimen). However, for one-step 1,115 ℃ specimen, a slight decrease in d33 was observed, emphasizing the importance of a high tetragonal (T) phase fraction for superior piezoelectric properties. Achieving a relative density above 84 % for samples sintered by the one-step sintering process was challenging. Conversely, two-step sintering significantly improved the relative density of KNNS-BNKZ ceramics up to 96 %, attributed to the control of AG nucleation in the first step and grain growth rate control in the second step. The quantity of AG nucleation was affected by the duration of the first step, determining the final microstructure. Despite having a lower T phase fraction than that of the one-step 1,100 ℃ specimen, the two-step specimen exhibited higher piezoelectric coefficients (d33 = 574 pC/N and kp = 0.5) than those of the one-step 1,100 ℃ specimen due to its higher relative density. Performance evaluation of magnetoelectric composite devices composed of one-step and two-step specimens showed that despite having a higher g33, the magnetoelectric composite with the one-step 1,100 ℃ specimen exhibited the lowest magnetoelectric voltage coefficient, due to its lowest kp. This study highlights the essential role of phase fraction and relative density in enhancing the performance of piezoelectric materials and devices, showcasing the effectiveness of the two-step sintering process for controlling the microstructure of ceramic materials containing volatile elements.

Study on the Numerical Analysis of Nuclear Reactor Kinetics Equations (원자로 동특성 방정식의 수치해석에 관한 연구)

  • Jae Choon Yang
    • Nuclear Engineering and Technology
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    • v.15 no.2
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    • pp.98-109
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    • 1983
  • A two-step alternating direction explicit method is developed to solve the space-dependent reactor kinetics equations in two space dimensions. As a special case in the general class of alternating direction implicit methods, this method is analysed for accuracy and stability. To test the validity of this method it is compared with the implicit-difference method used in the TWIGL program. It is shown that the two methods are closely related. The time dependent neutron fluxes of the pressurized water reactor (PWR), during control rod insertion, and, of the CANDU-PHW reactor, in case of postulated loss of coolant accident, are obtained from the numerical calculation results.

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A Study on Internship Program Development for Fashion Industry - Focused on Internship Activation Method of Fashion Industry and Government- (패션산업 인턴십 프로그램 개발에 관한 연구 - 패션기업과 정부의 인턴십 활성화 방안을 중심으로 -)

  • Yu, Ji-Hun;Chung, Sang-Gil
    • The Research Journal of the Costume Culture
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    • v.13 no.5 s.58
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    • pp.699-711
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    • 2005
  • This study was following one of 'A study on the consciousness of fashion industries internship'. The purposes of this study were to develop the internship program which focused on practical use to introduce and carry out for fashion industries, and secondly to propose some regime for government to activate fashion internship. Reference searching method and depth interviewing method were used for this study. The results were as follows : Fashion industry internship was grouped into two classes, 'on-the-job training'; educating students fields and 'talent hunting'; selecting good persons. Internship of industry-academic world was classified into two types; the one is 'credit type' which has curriculums between universities and industries and the other is 'non-credit type' which has not any credit and is operated by industry own system. This study provided the development courses of pragmatic program to perform internship systematically and it also provided the program models for guide line in fashion industries. Six grades such as ready step, introduction step, selection step, management step, evaluation step and feed-back step were proposed for the internship program development steps of fashion industries. A virtual organization, 'The Fashion Industry and Academy Association' was proposed as a policy for activating internship between universities, industries and government.

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Design of Prototype Rotary-Lineat Step Motor by the Finite Element Method (유한 요소법에 의한 2자유도 스텝모터의 설계)

  • 정태경;한송엽;원종수
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.35 no.12
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    • pp.567-572
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    • 1986
  • In this paper, a new type of step motor with two degree of mechanical freedom, which is named rotary-Linear Step Motor(RLSM), is presented. Its rotor axis can perform linear and rotary motions either separately or simultaneously. This paper discribes the design of RLSM using finite element method in which the magnetic saturation effect of the iron core is taken into account. The design parameters such as torques, forces and inductances are obtained from the computed magnetic vector potentials. A new type of Rotary-Linear Step Motor was constructed. The calculated parameters agree well with measurements.

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Fault Detection Using Propagator for Kalman Filter and Its Application to SDINS

  • Yu, Jae-Jong;Lee, Jang-Gyu;Park, Chan-Gook
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.978-983
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    • 2003
  • In this paper, we propose a fault detection method for extended Kalman filter in decentralized filter structure. To detect a fault, a consistency between filter output and a monitoring signal is tested. State propagators are used to obtain the monitoring signal. However, the output of state propagator increases in magnitude and finally diverges as time runs. To solve such problem, two-propagator method was proposed for linear system. Two propagators are reset by Kalman filter output, alternatively, to avoid divergence. But a test statistics change abruptly at the reset instant in that method. Hence a N-step propagator method is proposed to fix up the problem. In the N-step propagator, only time propagations are performed from k-N+1 step to k step without measurement updates. A test statistics are defined by errors and its covariance between extended Kalman filter and N-step propagator. These fault detection methods are applied to integrated strapdown inertial navigation system (SDINS). By computer simulation, it is shown that the proposed methods detect a fault effectively.

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